Quantification of epidermal growth factor receptor dynamics and interactions in living cells by fluorescent correlation and cross correlation spectroscopy

... for studying rafts is interrupting rafts by changing the amount of cholesterol in cells, which includes increasing its amount by adding in extra cholesterol and extracting it by drug treatments ... thereby enabling the exposure of the ligand-binding pocket of domain L1 and L2 The dimerization arm in domain CR1 is known to facilitate the formation of dimers, which leads to the kinase domain of ... functions of EGFR such as binding with ligands and subsequent activation Interestingly, again, opposing effects were observed in different settings of experiments In artificial 20 membranes, the binding...

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Rapid detection of epidermal growth factor receptor mutations with multiplex PCR and primer extension in lung cancer pdf

... Coulter Inc., Fullerton, CA, USA) according to manufacturer instructions Multiplex PCR and primer extension analysis of mutations in EGFR-216 promoter region and exons 18, 19, 20, and 21 Multiplex PCR ... amount of work and time required With this method, -216 promoter region and exons 18-21 of the EGFR gene were amplified with multiplex PCR in a single tube and the detection of mutations in the EGFR ... Feng Z, Roth JA, Herz J, Minna JD, Gazdar AF: Clinical and biological features associated with epidermal growth factor receptor gene mutations in lung cancers J Natl Cancer Inst 2005, 97(5):339-346...

Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf

... system may be one of the mechanisms responsible for drug -induced apoptosis in a variety of JNK activation is critical for AG1478 -induced apoptosis cancer cells of different histotype [51] Chang ... RA & Davis RJ (2000) Requirement of JNK for stress -induced activation of the cytochrome c-mediated death pathway Science 288, 870–874 JNK activation is critical for AG1478 -induced apoptosis 49 ... Authors Journal compilation ª 2009 FEBS K Takeuchi et al JNK activation is critical for AG1478 -induced apoptosis A B a Fig Induction of apoptosis by AG1478 (A) PC-9 cells were seeded into a 96-well...

Báo cáo khoa học: Mutation of epidermal growth factor receptor is associated with MIG6 expression docx

... regulatory mechanism of MIG6 in relation to the EGFR mutation The present study is the ﬁrst to show the association of MIG6 expression with the EGFR mutation in cancer MIG6 ⁄ RALT is known to be ... EGFR mutation and MIG6 expression T Nagashima et al Introduction Epidermal growth factor receptor (EGFR) is a membrane tyrosine kinase that is involved in the regulation of a wide variety of biological ... compilation ª 2009 FEBS 5247 EGFR mutation and MIG6 expression A T Nagashima et al B Fig Comparison of MIG6 expression and phosphorylation of signaling molecules (A) MIG6 expression levels in eight...

Báo cáo khoa học: " Assessment of Epidermal Growth Factor Receptor (EGFR) expression in human meningioma" docx

... Immunohistochemical staining intensity scores A) Meningiomas stained with anti-EGFR antisera showing negative staining B) 1+ staining C) 2+ staining D) 3+ staining Original magnification for ... Beguinot L, Macchia V: Epidermal growth factor receptor in human brain tumors J Endocrinol Invest 1992, 15(1):31-37 Johnson MD, Horiba M, Winnier AR, Artiaga CL: The epidermal growth factor receptor ... percentages of SP were estimated by inspection and scored from to 5, (< 20% of the sample exhibiting staining); (21-40% of the sample exhibiting staining); (41-60% of the sample exhibiting staining);...

Báo cáo y học: "Role of epidermal growth factor receptor activation in regulating mucin synthesis" pdf

... expression of the MUC5AC mucin (a predominant airway mucin) gene Interaction of EGFR with its ligands led to EGFR tyrosine kinase phosphorylation, and a selective EGFR tyrosine kinase inhibitor ... virus-induced goblet-cell metaplasia in pathogen-free rats, developing mucin- containing cells did not incorporate [3H]thymidine, suggesting that cell mitosis was not involved in synthesis of mucin ... expressed TNF-α An interleukin-8-blocking antibody inhibits interleukin-13-induced neutrophil recruitment and mucin production Thus, interleukin-13 causes goblet-cell metaplasia indirectly by neutrophil...

Báo cáo hóa học: " Epidermal growth factor receptor gene copy number in 101 advanced colorectal cancer patients treated with chemotherapy plus cetuximab" doc

... difference in PFS was also found in patients treated with Cetuximab plus chemotherapy as second or more line with or without an increased EGFR-GCN (p = 0.03) In our population 89 /101 patients were treated ... EGFR -gene copy number (GCN) in 31 selected patients with metastatic CRC treated with Cetuximab or Panitumumab Eight out of nine patients who obtained a partial response had an increased EGFR gene ... EGFR-GCN Table 1: FISH Data Patterns FISH Patients % Increased EGFR gene copy number in >50% of cells 56 /101 56 EGFR gene copy number ...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer ppt

... Mutations of the epidermal growth factor receptor gene and related genes as determinants of epidermal growth factor receptor tyrosine kinase inhibitors sensitivity in lung cancer Cancer Sci 98, ... Kinetic analysis of epidermal growth factor receptor somatic mutant proteins shows increased sensitivity to the epidermal growth factor receptor tyrosine kinase inhibitor, erlotinib Cancer Res 66, ... homology in the C-terminal regulatory domain are relatively low, being 33, 24 and 28%, respectively regulin and epigen); (b) those that bind to EGFR and ERBB4 (including betacellulin, heparin-binding...

Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... there could be a link between EGF-induced reduction in Bid and tBid levels and the ubiquitin ligase activity of Itch In this study, we ﬁrst examined the ability of Itch to interact with Bid and ... proteins Bim and Bak and the C-terminal fragment of Bid The ubiquitin ligases responsible for the ubiquitylation are in most cases not known [44] Here, we have identiﬁed Itch as the ubiquitin ligase ... Azakir et al Itch promotes tBid degradation Fig tBid, the active, apoptotic form of Bid, interacts with the ubiquitin ligase Itch, which leads to its degradation and proteasomedependent degradation...

Báo cáo khoa học: Calmodulin-mediated regulation of the epidermal growth factor receptor doc

... Nck [72] The binding of these proteins could prevent the interaction of the Ca2+ ⁄ CaM complex to the receptor if the CaMBD were occluded at least in part Nevertheless, the docking of these adaptor ... internalization and the subsequent degradation of the receptor in transfected human embryonic kidney cells [50] Direct regulation of the EGFR by CaM The direct regulation of the EGFR upon binding of the Ca2+ ... activation of the EGFR, where the C-terminal lobe of the kinase domain of one of the monomers forming the dimeric receptor interacts with the N-terminal lobe of the apposed monomer [19,47,84] Of relevance...

Báo cáo khoa học: Epidermal growth factor receptor-regulated miR-125a-5p – a metastatic inhibitor of lung cancer potx

... the epidermal growth factor (EGF) receptor ⁄ extracellular signalregulated kinase ⁄ and EGF receptor ⁄ Akt pathway for proliferation Mol Cancer Ther 3, 46 5–4 72 Amann J, Kalyankrishna S, Massion ... motility, and invasion It is also of great interest and importance to note that about one-third of the human lung cancer samples that we examined revealed signiﬁcant downregulation of miR-12 5a- 5p ... Cells and cultures The human lung cancer cell line A5 49 was obtained from the American Type Culture Collection (Manassas, VA, USA) and maintained in Ham’s F12K medium (Invitrogen, Carlsbad, CA,USA)...

Báo cáo khoa học: Phosphorylation of Hrs downstream of the epidermal growth factor receptor docx

... before the cells were washed and Hrs was immunoprecipitated from the lysate We chose to stimulate the cells with the growth factors for min, as the growth factor- stimulated phosphorylation of Hrs ... hypertonic media prevents the EGF-induced phosphorylation of Hrs This suggests that the phosphorylation of Hrs takes place on endosomes [4] As the activation of the EGF receptor occurs efﬁciently ... 3–4), the phosphorylation was much weaker than that detected at 37 °C, supporting the idea that endocytosis of the receptor/ ligand complex is important for the phosphorylation of Hrs Nevertheless,...

Báo cáo khoa học: "Topical application of epidermal growth factor accelerates wound healing by myofibroblast proliferation and collagen synthesis in rat" pot

... 21-01 rof deniatniam eb ot sah rotpecer sti dna FGEhr neewteb noitcaretni eht taht detroper saw tI noisicni eht fo yad eht no gninnigeb syad 41 rof h 21 yreve slamina eht ot yllacipot deretsinimda ... eussit racs evissecxe fo noitamrof eht gnisaerced ni elor a syalp ti saerehw ,ssecorp riaper dnuow eht secnahne FGE deilppa yllacipot ,elpmaxe roF ]91[ noitamrof racs no ecneulfni yna gnivah ... dna ,enelyx ni deziniffaraped ,h rof C 34 ta detabucni erew snoitces ehT sedils detaoc nisyl-ylop no detnuom dna tuc erew snoitces kciht mm eviF niffarap ni deddebme dna ,nilamrof lartuen %01 ni...

Báo cáo khoa học: " The epidermal growth factor receptor (EGFR) in head and neck cancer: its role and treatment implications" pptx

... signal-regulated kinases: association with epidermal growth factor receptor/ transforming growth factor alpha expression in head and neck squamous carcinoma and inhibition by anti -epidermal growth factor receptor ... transforming growth factor α and epidermal growth factor receptor in patients with squamous cell carcinoma of the head and neck Cancer 1996, 78:1284-1292 Baumann M, Krause M: Targeting the epidermal growth ... Radiation-induced release of transforming growth factor α activates the epidermal growth factor receptor and mitogen-activated protein kinase pathway in carcinoma cells, leading to increased...

báo cáo khoa học: "Sustained complete remission of human epidermal growth factor receptor 2-positive metastatic breast cancer in the liver during long-term trastuzumab (Herceptin) maintenance therapy in a woman: a case report" docx

... Sustained complete remission of human epidermal growth factor receptor 2-positive metastatic breast cancer in the liver during long-term trastuzumab (Herceptin) maintenance therapy in a woman: a case ... will influence whether a patient achieves long-lasting remission on maintenance trastuzumab therapy We also speculate that the specific localization of breast cancer metastases may be a factor ... to eight years, and in all cases, maintenance therapy was based on trastuzumab One of these cases also illustrates the risk of withdrawing trastuzumab treatment when the patient had experienced...

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