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Genetic approaches to study liver development in zebrafish   core component sec13 in COPII complex is essential for liver development in zebrafish

Genetic approaches to study liver development in zebrafish core component sec13 in COPII complex is essential for liver development in zebrafish

Cao đẳng - Đại học

... 11 4 6.3 Results 11 4 6.3 .1 Sec13sq198 is a carboxyl-terminal truncated protein 11 4 6.3.2 Expression pattern of sec13 and sec31A in zebrafish 11 6 6.3.3 Sec13 and Sec31A co-localized ... zebrafish120 6.3.4 Sec13sq198 fails to form a complex with Sec31A 12 4 6.3.4 .1 Sec13sq198 fails to co-localize with Sec31A in vivo 12 6 6.3.4.2 Sec13sq198 fails to be immunoprecipitated with Sec31A ... mutation 11 0 5.4 Discussion 11 1 Chapter Study of the Sec13sq198 mutant protein at the cellular and biochemical levels 11 3 6 .1 Introduction 11 3 6.2 Materials...
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Expression analysis of GFAP like gene in zebrafish embryogenesis

Expression analysis of GFAP like gene in zebrafish embryogenesis

Tổng hợp

... 2.5 .1 Microinjection 2.5.2 Making of sub-slides 2.5.3 Cryostat sectioning 2.5.4 Mounting and Photography 35 36 36 36 37 37 38 39 39 3 .1 3 .1. 1 3 .1. 2 3.2 3.3 3.3 .1 3.3.2 41 41 41 43 49 51 51 RESULTS ... P1 413 6; mouse GFAP # P03995; human peripherin #P 412 19; mouse peripherin #P153 31; goldfish plasticin #P 313 393; zebrafish plasticin # AAC34932; zebrafish desmin # AAB03 217 ; human desmin #P176 61; ... Zebrafish 1. 3.2 Development of the zebrafish CNS 1. 3.2 .1 Specification of the neuroectoderm 1. 3.2.2 Regionalization of the neural tube 1. 3.2.3 Neurogenesis 1. 4 Objectives of the present Study 12 12 14 ...
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Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

Báo cáo khoa học

... 68.0 0 1 2 0 ⁄ 8.0 ⁄ 4.0 0 0 0 0 ⁄ 4.0 1 2 0 31 14 25 – – 14 38 24 11 15 – – 329 – 18 – – – 45 0 0 – – – – 11 – – – 2 .11 – – – – – 0.76 3.40 2.33 – 1. 33 – – 64.34 – 71. 57 – – 4.96 – – 1. 50 – 11 .29 ... Acad Sci USA 10 1, 11 707 11 712 11 Corcoran EE & Means AR (20 01) Defining Ca2+ ⁄ calmodulin-dependent protein kinase cascades in transcriptional regulation J Biol Chem 276, 2975–2978 12 Ikura M, ... – + – + + + – – n.a – – – n.a n.a – 17 ⁄ 16 .0 22 ⁄ 20.7 0 0 ⁄ 1. 8 0 ⁄ 2.8 ⁄ 1. 8 ⁄ 0.9 ⁄ 4.7 ⁄ 0.9 ⁄ 1. 8 ⁄ 6.6 ⁄ 0.9 ⁄ 1. 8 ⁄ 1. 8 ⁄ 1. 8 ⁄ 0.9 12 4 76 – – – – 10 .89 6.97 – – – – – – +++ ++ – – +...
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báo cáo khoa học:

báo cáo khoa học: "Analysis of the expression pattern of the BCL11B gene and its relatives in patients with T-cell acute lymphoblastic leukemia" doc

Báo cáo khoa học

... Oncology 2 010 , 3:44 http://www.jhoonline.org/content/3 /1/ 44 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Oh YK, Park TG: siRNA delivery systems for cancer treatment Adv Drug Deliv Rev 2009, 61: 850-862 ... electrophoresis Line 1- 2: BCL11B (19 3bp), line 3: DNA ladder, line 4-5: BCL2L1 (202 bp), line 6-7: CREBBP (206 bp), line 8-9: SPP1 (2 41 bp), line 10 -11 : TNFSF10 (19 0 bp) F: Relative expression ... BCL11B gene through inv 14 q 11. 2q32. 31 results in the expression of BCL11B-TRDC fusion transcripts and is associated with the absence of wild-type BCL11B transcripts in TALL Leukemia 2005, 19 :2 01- 208...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot

Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot

Báo cáo khoa học

... data library Nucleic Acids Res 14 , 5 10 20 Bilofsky, H.S., Burks, C & The GenBank data bank (19 88) Nucl Acids Res 16 , 18 61 18 64 21 Pearson, W.R & Lipmann, D.J (19 88) Improved tools for biological ... Carcinogenesis 18 , 15 69– 17 75 18 Liang, P & Pardee, A.B (19 92) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction Science 257, 967–9 71 19 Hamon, G.H & Cameron, G.N (19 86) ... The second clone of about 0.9 kb had 10 0% homology to part c c Hypervitaminosis 1. 61 ± 0 .12 (5) 1. 60 ± 0.22 (5) b 78.08 ± 15 .40 (3) a 4276 ± 13 34 (5) a 44 .14 ± 6.05 (5) a 262.74 ± 35.55 (5) a...
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Báo cáo khoa học: High and complementary expression patterns of alcohol and aldehyde dehydrogenases in the gastrointestinal tract Implications for Parkinson’s disease docx

Báo cáo khoa học: High and complementary expression patterns of alcohol and aldehyde dehydrogenases in the gastrointestinal tract Implications for Parkinson’s disease docx

Báo cáo khoa học

... and rat Adh1, Adh3, Adh4 and Aldh1 Probe Gene Species Sequence 5’- to 3’ Adh1 Adh1 -1 Adh1-2 Adh3 Adh3 Adh4 Adh4 -1 Adh4-2 Adh4-3 Aldh1 Random Adh1 (class I Adh) Adh1 (class I Adh) Adh1 (class I ... B6.Adh1– ⁄ – congenic strain was provided by NIH grant AA 118 28 10 11 12 References 13 Farrer MJ (2006) Genetics of Parkinson disease: paradigm shifts and future prospects Nat Rev Genet 7, 306– 318 ... kinetic FEBS Journal 274 (2007) 12 12 12 23 ª 2007 The Authors Journal compilation ª 2007 FEBS 12 21 Expression of ADH and ALDH in the rodent GI tract 17 18 19 20 21 22 23 24 25 26 27 28 29 30 M...
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Báo cáo khoa học: Expression of the pyrG gene determines the pool sizes of CTP and dCTP in Lactococcus lactis doc

Báo cáo khoa học: Expression of the pyrG gene determines the pool sizes of CTP and dCTP in Lactococcus lactis doc

Báo cáo khoa học

... CJ407 CJ 410 CJ 411 CJ 413 CJ 418 CJ420 Plasmids pCJ31B pLB65 pLB86 pCR2 .1- TOPO pAK80 Relevant description Reference or source L lactis ssp cremoris strain MG1363 cdd MB109/pAK80 MB109 pyrG::ISS1 CJ295/pLB65 ... Microbiol 55, 311 9 312 3 13 Johansen, E & Kibenich, A (19 92) Characterization of Leuconostoc isolates from commercial mixed strain mesophilic starter cultures J Dairy Sci 75, 11 8 611 91 14 Israelsen, ... at 15 C or two days at 30 C Growth at 15 C Strain Genotype CJ406 CJ 418 CJ 410 CJ405 CJ 413 CJ3 81 CJ388 CJ340 CJ233 cdd cdd cdd cdd cdd cdd cdd cdd cdd pyrG::ISS1 pyrG::ISS1 pyrG::ISS1 pyrG::ISS1...
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Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines ppt

Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines ppt

Sức khỏe phụ nữ

... receptors Nippon Sanka Fujinka Gakkai Zasshi 19 85, 37(7) :11 03 -11 11 21 Nishida M: The Ishikawa cells from birth to the present Hum Cell 2002, 15 (3) :10 4 -11 7 22 Johnson SM, Maleki-Dizaji M, Styles ... cancer cell line Science 19 77, 19 6 :14 56 -14 58 33 Pater MM, Pater A: Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix Virology 19 85, 14 5: 313 - 318 34 Yee C, Krishnan-Hewlett ... MMP -11 Schröpfer et al BMC Cancer 2 010 , 10 :553 http://www.biomedcentral.com /14 71- 2407 /10 /553 Page of 12 Table Expression levels of MMP mRNA in gynecological cancer cell lines Ishikawa HEC -1- A...
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Báo cáo khoa học: Molecular identification and expression study of differentially regulated genes in the Pacific oyster Crassostrea gigas in response to pesticide exposure doc

Báo cáo khoa học: Molecular identification and expression study of differentially regulated genes in the Pacific oyster Crassostrea gigas in response to pesticide exposure doc

Báo cáo khoa học

... CF36 912 8 CF36 913 6 CF36 913 7 CF36 914 0 CF36 912 5 CF36 912 7 CF36 914 3 AF177226 AF177226 CF36 913 2 CF36 914 2 CF36 912 6 CF36 913 4 CF36 914 7 CF36 913 1 CF36 913 5 CF36 914 1 CF36 913 3 CF36 912 9 CF36 914 5 CF36 914 6 CF36 914 4 ... 6e -18 3e-9 2e-9 3e -10 CF36 917 8 CF36 917 9 CF36 918 1 CF36 918 5 CF36 918 7 CF36 918 2 2e-4 3e -15 7e -10 4e-40 CF36 917 5 CF36 918 4 CF36 918 3 CF36 917 6 1e-4 9e-6 2e-52 2e-29 CF36 917 7 CF36 918 0 CF36 918 6 CF36 918 8 ... response to pesticide exposure A 14 12 10 12 10 11 12 13 14 15 16 B 10 T0 T7 T14 T 21 T30 14 C 12 10 2 FEBS Journal 272 (2005) 390–403 ª 2005 FEBS 10 11 12 395 Oyster response to pesticide exposure...
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Báo cáo sinh học:

Báo cáo sinh học: "High ERCC1 expression predicts cisplatin-based chemotherapy resistance and poor outcome in unresectable squamous cell carcinoma of head and neck in a betel-chewing area" pdf

Hóa học - Dầu khí

... Chiu et al Journal of Translational Medicine 2 011 , 9: 31 http://www.translational-medicine.com/content/9 /1/ 31 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Vokes EE, Weichselbaum RR, Lippman ... 11 (45.8%) 13 (54.2%) oropharynx 21 (36.8%) 10 (47.6%) 11 (52.4%) 1. 58 (0.35, 7.07) 0.549 hypopharynx/Larynx 12 ( 21. 1%) 10 (83.3%) (16 .7%) 0.096 (0.007, 1. 33) 0.0 81 Stage IVa 10 (17 .5%) (70.0%) ... 47(82.5%) 24 ( 51. 1%) 23 (48.9%) 1/ 2 (10 .5%) (10 0%) (0%) 3/4 51 (89.5%) 25 (49.0%) 0. 319 3.33 (0.39, 27.89) 26 ( 51. 0%) 0.027* 0.276 Indeterminate 0.999 N stage negative 12 ( 21. 1%) ( 41. 7%) (58.3%)...
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báo cáo hóa học:

báo cáo hóa học: " Maximal COX-2 and ppRb expression in neurons occurs during early Braak stages prior to the maximal activation of astrocytes and microglia in Alzheimer''''s disease" pdf

Hóa học - Dầu khí

... European Community (ADIT programme, LSHBCT- 511 977 to AJMR) http://www.jneuroinflammation.com/content/2 /1/ 27 20 21 References 10 11 12 13 14 15 16 17 18 19 Nagy Z, Esiri MM, Cato AM, Smith AD: Cell ... (years) PMI ± SD (hrs:min) A B C total 4/3 69 ± 12 7:00 ± 4:00 3/3 79 ± 8:30 ± 3:00 11 3/8 85 ± 10 7:00 ± 2:30 16 2 /14 82 ± 10 6:00 ± 2:00 40 12 /28 80 ± 11 6:30 ± 2:30 Page of (page number not for ... Neuroreport 19 97, 8 :11 61- 116 4 22 23 24 25 26 Pasinetti GM, Aisen PS: Cyclooxygenase-2 expression is increased in frontal cortex of Alzheimer's disease brain Neuroscience 19 98, 87: 319 -324 Combrinck...
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báo cáo hóa học:

báo cáo hóa học:" High ERCC1 expression predicts cisplatin-based chemotherapy resistance and poor outcome in unresectable squamous cell carcinoma of head and neck in a betel-chewing area" pdf

Hóa học - Dầu khí

... Chiu et al Journal of Translational Medicine 2 011 , 9: 31 http://www.translational-medicine.com/content/9 /1/ 31 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Vokes EE, Weichselbaum RR, Lippman ... 11 (45.8%) 13 (54.2%) oropharynx 21 (36.8%) 10 (47.6%) 11 (52.4%) 1. 58 (0.35, 7.07) 0.549 hypopharynx/Larynx 12 ( 21. 1%) 10 (83.3%) (16 .7%) 0.096 (0.007, 1. 33) 0.0 81 Stage IVa 10 (17 .5%) (70.0%) ... 47(82.5%) 24 ( 51. 1%) 23 (48.9%) 1/ 2 (10 .5%) (10 0%) (0%) 3/4 51 (89.5%) 25 (49.0%) 0. 319 3.33 (0.39, 27.89) 26 ( 51. 0%) 0.027* 0.276 Indeterminate 0.999 N stage negative 12 ( 21. 1%) ( 41. 7%) (58.3%)...
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Báo cáo khoa học:

Báo cáo khoa học: " Linkage disequilibrium pattern of the ATM gene in breast cancer patients and controls; association of SNPs and haplotypes to radio-sensitivity and post-lumpectomy local recurrence" doc

Báo cáo khoa học

... http://www.ro-journal.com/content/2 /1/ 25 25 26 31 27 28 29 30 32 33 38 67 11 12 10 16 39 34 20 15 22 48 42 24 51 52 53 47 43 44 55 46 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 46 ... GG GA TT TC 69 69 61 61 19 19 0 1 > 0.0 01 CC CT 11 11 1 11 13 0.009 GG GA AA 14 21 28 47 15 0.027 CC CT 75 12 1 0 0.043 CC CT 64 20 0 0.0 01 CC CT 51 35 2 0 0.002 CC CT 32 25 16 0.022 a) Pleural ... 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 36 ATG 19 14 17 21 45 23 18 3536 13 40 49 50 56 41 54 37 ATM gene Ser-367 Ser -18 93 Ser -19 81 P P P 45 10 11 12 13 14 15 ...
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Báo cáo y học:

Báo cáo y học: " Differential binding and co-binding pattern of FOXA1 and FOXA3 and their relation to H3K4me3 in HepG2 cells revealed by ChIP-seq" docx

Báo cáo khoa học

... 0 0 1 1 1 1 3 3 3 3 2 2 2 1 1 1 1 3 3 3 3 G T T T A C T T 717 5432 C G C T G 1 1 1 1 1 1 1 1 1 1 1 3 3 3 3 3 3 3 3 0 0 0 2 2 2 2 2 2 2 2 2 2 1 1 1 1 1 1 1 0 0 0 0 0 0 0 1 1 1 1 1 1 C A C G 717 5424 ... A A 1 0 0 0 1 0 0 0 0 2 1 2 2 2 2 2 2 3 0 2 2 2 0 0 1 1 1 0 0 0 3 1 2 0 0 0 1 3 2 1 3 1 1 3 1 2 0 0 1 3 2 1 0 0 0 0 2 0 0 0 0 1 1 1 1 3 3 3 3 1 1 3 3 1 1 1 1 1 1 0 0 2 0 3 2 0 0 0 0 3 1 1 0 0 ... FOXA1 ChIP DNA: 3 CGTGTTTACTT[T/C] CGTGTTTACTTT 1 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 2 2 1 1 2 2 2 2 2 1 2 2 3 3 2 2 0 0 2 2 2 0 0 0 0 0 0 3 3 3 3 1 1 1 1 1 1 1 1 1 0 0 0 0 0 0 0 0 0 3 3 3 3 1 1 1 1...
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báo cáo khoa học:

báo cáo khoa học: "Low-level expression of HER2 and CK19 in normal peripheral blood mononuclear cells: relevance for detection of circulating tumor cells" doc

Báo cáo khoa học

... 96.5% 10 10 10 10 10 10 10 10 PE Log 10 10 PMT1 Log 12 Cell number by B2M 10 Cytokeratin 19 2 CD14 CD16 CD3 /19 total PBMC B2M Cell Equivalents (X10 ) 14 CK19 Cell Equivalents/Cell (X 10 –8) F CK19 ... 14 PE 1. 5% 10 0 10 NK cells T and B cells 10 10 23 PE Log 10 10 10 PE Log C D Monocytes after sort T and B cells after sort 10 10 10 PECY5 Log FITC Log CD16 10 FITC 0 .1% CD14 PE 96.4% 10 10 CD3 ... 10 (page number not for citation purposes) Journal of Hematology & Oncology 2008, 1: 2 10 11 12 13 14 15 16 17 18 19 detection of occult bone marrow micrometastases J Clin Oncol 19 91, 9 :17 49 -17 56...
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báo cáo khoa học:

báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc

Báo cáo khoa học

... purposes) BMC Plant Biology 2009, 9:69 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 aquatic macrophytes differing in salt tolerance Biol Plant 2007, 51: 110 -11 5 Vitart V, Baxter I, Doerner P, ... 2000, 12 4 :15 3 -16 2 Page 24 of 25 (page number not for citation purposes) BMC Plant Biology 2009, 9:69 94 95 96 97 98 99 10 0 10 1 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9 http://www.biomedcentral.com /14 71- 2229/9/69 ... 3.00E -14 606 574 686 5 81 152 295 1. 89 1. 00E -16 305 0.47 0.93 3.00E-23 2.00E-74 727 407 0.70 8.00E -12 4 814 0.70 1. 00E -14 0 803 0.23 2.00E-66 804 0.47 7.6 225 0.23 2.00E-74 486 0.70 1. 00E- 31 4 41 0.70...
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Báo cáo y học:

Báo cáo y học: "Effects of ischemic pre- and postconditioning on HIF-1a, VEGF and TGF-b expression after warm ischemia and reperfusion in the rat liver" ppsx

Báo cáo khoa học

... 14 2 cases Ann Surg 19 89, 209: 211 - 218 Hannoun L, Borie D, Delva E, Jones D, Vaillant JC, Nordlinger B, Parc R: Liver resection with normothermic ischaemia exceeding h Br J Surg 19 93, 80 :11 61- 116 5 ... The authors declare that they have no competing interests Received: 10 January 2 011 Accepted: 19 July 2 011 Published: 19 July 2 011 References Fong Y, Fortner J, Sun RL, Brennan MF, Blumgart LH: ... and a primer of the target, i.e., HIF-1a (Rn00577560_m1), TGF-b (Rn00572 010 _m1) and VEGF-A (Rn43 313 48), and a primer of the housekeeping gene, 18 S (4 319 413 ), all purchased from Applied Biosystems...
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Báo cáo sinh học:

Báo cáo sinh học: "Cloning, chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes" pdf

Báo cáo khoa học

... (a.a.) ADRP 459 M6PRBP1 439 PLIN 516 Identity PAT -1 with domain ADRP (a.a.) 9 10 0 37.3% 23 11 4 19 .2% 17 10 8 Identity 33-mer with motif ADRP (a.a.) 12 5 15 7 60.9% 14 3 17 5 40.2% 12 6 15 8 Identity PAT-C ... 78– 81 274–277 428–4 31 433–436 488–4 91 513 – 516 Pig — RRLS RRQS RRET RRGS RRVS RKKS 78– 81 274–277 433–436 488–4 91 513 – 516 Cattle — — RRLS RRRS RRGS RRVS RKKS 78– 81 274–277 433–436 494–497 519 –522 ... with domain ADRP (a.a.) 17 5–4 01 51. 5% 19 3–422 18 .2% 17 6–407 Identity with ADRP 36.9% 17 .2% the following cytogenetic localization i.e SSC7q 11- q14 for PLIN The gene M6PRBP1was mapped to SSC2 at...
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Báo cáo sinh học:

Báo cáo sinh học: " The pattern of genetic and environmental variation in relation to ageing in laying hens" ppt

Báo cáo khoa học

... environments (Clayton and Robertson, 19 66; Flock, 19 77; Liljedahl et al, 19 84 in laying hens; Rose and Charlesworth, 19 81a; Burla and Taylor, 19 82; Engstr6m et al, 19 89 in Drosophila medanogaster) ... 97, 17 3 -18 6 Rose MR, Charlesworth B (19 81b) Genetics of life history in Drosophila melanogaster II Exploratory selection experiments Genetics 97, 18 7 -19 6 Service PM, Hutchinson EW, Rose MR (19 88) ... Taylor CE (19 82) Increase of phenotypic variance in stressful environ73, 14 2 Robertson A (19 66) Genetics of changes in economic traits during the Clayton GA, laying year Br Poult Sci 7, 14 3 -15 1 Engstr6m...
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Application of bayesian model selection in fluorescence correlation spectroscopy (FCS) to WNT3EGFP secretion and diffusion in zebrafish embryos

Application of bayesian model selection in fluorescence correlation spectroscopy (FCS) to WNT3EGFP secretion and diffusion in zebrafish embryos

Thạc sĩ - Cao học

... (Thompson 19 91; Kinjo and Rigler 19 95): G3D,2p (τ) = N ( G2D,2p (τ) = 𝜏 1/ 2 ( 𝜏 )] 𝐾 1 τ 1 𝜏 1/ 2 +F2 (1 + τ ) [1 + ( 𝜏 )] 𝐾 D2 𝐷2 (1 − F2 ) (1 + 𝜏 1 𝜏 1 ) [1 + + G∞ (2 .15 ) ) τ 1 τ 1 [ (1 − F2 ... (𝜏) + G∞ (2. 21) ) τ 1 τ 1 [ (1 − F2 ) (1 + ) + F2 (1 + ) ] 𝑓𝑡𝑟𝑖𝑝 (𝜏) + G∞ (2.22) N τD1 τD2 (1 − F2 − F3 ) (1 + G3D,3p1t (τ) = N 1 𝜏 1 ) 𝜏 1/ 2 [1 + ( 𝜏 )] 𝐾 1 1 𝜏 −2 +F2 (1 + τ ) [1 + ( 𝜏 )] ... modified: 𝐺2𝐷 ,1 1 (𝜏) = G3D,2p1t (τ) = 𝜏 1 (1 + ) 𝑓𝑡𝑟𝑖𝑝 (𝜏) + 𝐺∞ (2.20) 𝑁 𝜏𝐷 𝜏 1/ 2 ( 𝜏 )] 𝐾 1 τ 1 𝜏 1/ 2 +F2 (1 + τ ) [1 + ( 𝜏 )] 𝐾 D2 𝐷2 (1 − F2 ) (1 + N ( G2D,2p1t (τ) = 1 𝜏 𝜏 1 ) [1 + 𝑓𝑡𝑟𝑖𝑝...
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