validation of a sensitive and speci ﬁ c real time pcr for detection and

Báo cáo khoa học: "Development of real time PCR for detection and quantitation of Dengue Viruses" potx

... [11,16], core [9] and Table 2: Nucleotide sequence of primers and probe used in the qRT -PCR assay Sequence Forward Primer Reverse Primer TaqMan MGB Probe 5'-GARAGACCAGAGATCCTGCTGTCT-3' 5'-ACCATTCCATTTTCTGGCGTT-3' ... malaria, Chikungunya, rickettsia and leptospira co-exist and are clinically indistinguishable In areas where other flaviviruses are circulating, IgM detection is not conclusive because of cross reactivity ... establishing a group specific RT -PCR assay This could have been because of lack of a suitable stretch of conserved nucleotides for designing the conventional TaqMan probes, earlier observed by...

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Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps

Danh mục: Báo cáo khoa học

... tgcagaaaattgatgctgct ttgcccaggttggtaatagc JOE-acctgggtgcggtacagaaccgt-BHQ 1a ggtaaaggggcttcggtatc tattggctccctgaatacgc Cy5-tggtggtgtagccactgtcccgt-BHQ 1a GenBank Accession number (Nucleotide position) ... sefA Name Sequence (5′ to 3′) S16R-F S16R -R Scom-FAM SfC-F SfC -R ST-JOE SsA-F SsA-R SE-Cy5 aggccttcgggttgtaaagt gttagccggtgcttcttctg FAM-aaccgcagcaattgacgttaccc-BHQ 1a tgcagaaaattgatgctgct ttgcccaggttggtaatagc ... real- time PCR assays have been successfully applied in the detection of bacterial pathogens in food products [11,12,24,25] A single real- time PCR assay was applied for specific detection of major...

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Báo cáo y học: " Prediction of conformational changes by single mutation in the hepatitis B virus surface antigen (HBsAg) identified in HBsAg-negative blood donors" ppsx

Danh mục: Báo cáo khoa học

... overcome the issues regarding detection failure by diagnostic assays and the global use of vaccines, particularly in endemic areas, as one possible mechanism of selecting escape mutants Acknowledgements ... mutation-induced conformational changes at the antigenic a determinant of its surface antigen [2,3,5] Selection of variants is usually indicated by certain serological markers, such as isolated anti-HBc, co-occurrence ... co-occurrence of both HBsAg and anti-HBs, and inconsistent HBsAg assay results [34] The presence of these variants poses potential threat to the success of vaccination and supply of safe blood...

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

Danh mục: Báo cáo khoa học

... inhibition and hemagglutinatin study The lectin is unique from that of other sialic acidspeci c lectins O-Acetyl sialic acid -speci c lectin was Ó FEBS 2003 A sialic acid speci c lectin from P jacquemontii ... in deriving the binding afﬁnity of the humoral agglutinin BSM contains the sialic acids, N-acetylneuraminic acid, N-glycolylneuraminic acid, N-acetyl 9-O-acetylneuraminic acid and, 8,9-di-O-acetylneuraminic ... Paulson, J .C (1985) Puriﬁcation and characterization of an O-acetyl sialic acid speci c lectin from a marine crab Cancer antennarius J Biol Chem 260, 8850–8856 13 Kawai, T Kato, A Higashi, H Kato,...

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Báo cáo khoa học: Hierarchical subfunctionalization of fabp1a, fabp1b and fabp10 tissue-speciﬁc expression may account for retention of these duplicated genes in the zebraﬁsh (Danio rerio) genome docx

Danh mục: Báo cáo khoa học

... involved in the binding at site of the human FABP1, and possibly in zebrafish FABP 1a and zebrafish FABP 1b, may reflect differences in the binding affinities of the zebrafish FABP 1a and zebrafish FABP 1b to ... zebrafish FABP 1a and FABP 1b (A) The 827 bp fabp 1a cDNA sequence, excluding the poly (A) tail, was determined by cloning and sequencing of 3¢-RACE and 5¢-RLM-RACE products The cDNA sequence contained ... mRNAs in adult zebrafish RT -PCR generated a fabp 1a and fabp 1b mRNA -speci c product from total RNA extracted from adult zebrafish intestine (I) No fabp 1a or fabp 1b mRNAspeci c product was generated...

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Báo cáo Y học: A complex fruit-speciﬁc type-2 ribosome-inactivating protein from elderberry (Sambucus nigra) is correctly processed and assembled in transgenic tobacco plants doc

Danh mục: Báo cáo khoa học

... between Cys259 of the A- chain ˚ and Cys4 of the B- chain of ricin (4.81 A in ricin), which form the disulﬁde bridge connecting both chains One can reasonably assume therefore that the A- and B- chain ... B- chain, the agglutination activity and carbohydrate-binding speci city, and RNA N-glycosylase activity of SNA-If and rSNA-If were compared As shown in Table 1, rSNA-If exhibits the same speci c ... in the catalytic activity of the A- chain and the carbohydratebinding activity of the B- chain are identical in SNA-I and SNA-If This explains why no difference could be observed between the catalytic...

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Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx

Danh mục: Điện - Điện tử

... 5'-TGTAAAACGACGGCCAGTAGCCTGTAC- http://www.virologyj.com/content/2/1/61 CCCAGCAT-3'; HSV-M13 reverse 5'-CAGGAAACAGCTATGACCTGGGCCTTCACGAAGA-3'] Cycling temperatures were the same as for the HSV realtime ... 'spiked' into a PCR reaction containing approximately 100 copies of bacteriophage λ DNA and a primer pair directed against λ DNA The performance of the PCR was monitored by quantitative real- time PCR ... most common aetiological agent of GUD [5] Studies of HSV-2 seroprevalence have found high rates in African-Americans [6] and in African populations in Uganda, Zimbabwe, Tanzania, Central African...

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báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf

Danh mục: Hóa học - Dầu khí

... 5'-TGTAAAACGACGGCCAGTAGCCTGTAC- http://www.virologyj.com/content/2/1/61 CCCAGCAT-3'; HSV-M13 reverse 5'-CAGGAAACAGCTATGACCTGGGCCTTCACGAAGA-3'] Cycling temperatures were the same as for the HSV realtime ... 'spiked' into a PCR reaction containing approximately 100 copies of bacteriophage λ DNA and a primer pair directed against λ DNA The performance of the PCR was monitored by quantitative real- time PCR ... most common aetiological agent of GUD [5] Studies of HSV-2 seroprevalence have found high rates in African-Americans [6] and in African populations in Uganda, Zimbabwe, Tanzania, Central African...

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Báo cáo y học: " Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene" doc

Danh mục: Báo cáo khoa học

... SYBR Green I-based real- time PCR TaqMan-based real- time PCR may be more specific than SYBR Green I-based real- time PCR because the former requires specific probes that bind with PPV DNA template, ... means of four replicates from each of four times) were relatively small (Table 1) Detection of PPV in clinical samples by real- time PCR and conventional PCR Real- time PCR and conventional PCR were ... primers and probe were: NS1-FP (forward primer): 5’-GAAGACTGGATGATGACAGATCCA-3’, NS1-RP (reverse primer): 5’-TGCTGTTTTTGTTCT TGCTAGAGTAA-3’ NS1-P (probe): FAM-AATGATGGCTCAAACCGGAGGAGA-BHQ1 The probe...

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báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot

Danh mục: Báo cáo khoa học

... CAGGCAGGTTAAGGCAAAGC/ CTAGCAAGGTACAGAAACGGC AAGCTCCCACCTGTCTGGAAA/ AACAGATTGCCGGAAGCCA CTTACGACGAGTTCAGATGCC/ TAAGTCCTCAACACGCATGC TGGAAACTCAACCTCCATCCA/ TTTCGTCCATTCCTTCACCTG 135 1.83 ± 0.09 114 1.70 ... EF 1a Elongation factor 1-alpha SGN-U207468 (At5 g60390.1) CCTGGTCAAATTGGAAACGG/ CAGATCGCCTGTCAATCTTGG 103 1.62 ± 0.08 AACAACTCACTCCTACACCGG/ GGTAGCACTAGAGACACAGCCTT CAGGCAGGTTAAGGCAAAGC/ CTAGCAAGGTACAGAAACGGC ... SGN-U208507 (At3 g12110.1) 2e-110 TGCACTCCCACATGCTATCCT/ TCAGCCGAAGTGGTGAAAGAG 114 1.75 ± 0.07 CYP Cyclophilin SGN-U207595 (At2 g21130.1) 1.9e-75 AGGCTCATCATTCCACCGTGT/ TCATCTGCGAACTTAGCACCG 111 1.64...

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Báo cáo y học: " Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA" pot

Danh mục: Báo cáo khoa học

... 5’-GCCAAAATTCGCAGTCCC-3’, 0.5 μM primer hbv460 (antisense) 5’-GATAGTCCAGAAGAACCAACAAGAAG-3’, 0.4 μM molecular beacon 5’-CG CGCGATGAGGCATAGCAGCAGGATGAAGAACG CGCG-3’ labelled with FAM and Dabcyl at ... assay for hepatitis B virus DNA and comparison with two commercial assays J Clin Microbiol 2000, 38:2897-2901 Lole KS, Arankalle VA: Quantitation of hepatitis B virus DNA by real- time PCR using ... of chronic hepatitis B virus infection J Clin Microbiol 1992, 30:1111-1119 Abe A, Inoue K, Tanaka T, Kato J, Kajiyama N, Kawaguchi R, et al: Quantitation of hepatitis B virus genomic DNA by real- time...

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Báo cáo y học: " The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus" potx

Danh mục: Báo cáo khoa học

... (bp) Product (bp) Nf CCCGGGTTGAAAAGCCTCGTGT 22 22 8a Nr GGCTTCTCCGGGTTTTTCTTCCTA 24 371f CCCGGGTTGAAAAGCCTCGTGT 22 371r TGTAACTTATCCTCCCTGAATCTG 24 a Length of product amplified by one step real- time ... specific and rapid method for detection and quantitation of PRRSV in field samples Materials and methods Virus strain and field samples CH- 1a (GenBank access number: AY032626) virus strain, CSFV, ... transcription for 30 at 50 C, followed by a PCR activation for at 94 C, 30 cycles of amplification (50 s at 94 C, 50 s at 56 C, and at 72 C) , and a final extension step at 72 C for The resulting PCR...

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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx

Danh mục: Báo cáo khoa học

... TCID50/cell .A significant difference in binding to HeLa in Figure Binding studies for DAF and CAR Binding studies for DAF and CAR A) MOI 0.5 TCID50/cell ofCVB2O, EV7W and CVB5 was incubated with ... limited amount of cells was also investigated A decreasing number of cells were incubated with CVB5 at a concentration of MOI 0.05 TCID50/cell and significant differences in binding to HeLa in comparison ... clinical isolate and performed the radioactive measured binding assay SI participated in handling and analysing recombinant cell lines AML was involved in the study design, draft and revision of...

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Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps

Danh mục: Báo cáo khoa học

... detection of H5N1 influenza A virus of both clade and directly in clinical specimens, and evaluated it with a large number clinical samples Using this assay, reliable diagnostic results can be obtained ... study Name Sequencea Nucleotideb Sense 5’-TTGGTTACCATGCAAACAAYT-3’ 91-111 Antisense 5’-TRTCTTGGGCRTGTGTAACA-3 152-171 Probe 119-143 5’-FAM-CAGGTTGACACAATAATGGAAAAGBHQ3-3’ a Y = T or C, R = A or ... rRT -PCR for detection of clade and H5N1 viruses in a large number of clinical specimens (n = 58) The assay described here has been established within the laboratories of the South East Asia Infectious...

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Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR

Danh mục: Môi trường

... ctcctcccacaaatcaggac Saito et al., 200 3b QMF QMR QMT (Probe) agacgcacgctcacctcaa gagcagttcacgaaatcc atacgctcttactgtttccggccgcc in this study BACT1369F PROK1492R TM1389BACT2 (Probe) cggtgaatacgttcycgg ggwtaccttgttacgactt ... ggwtaccttgttacgactt cttgtacacaccgcccgtc Suzuki et al., 2000 Real- time PCR The microcystin-degrading bacteria were quantified by real- time PCR using QMF and QMR oligonucleotide primers, and a QMT TaqMan ... microcystin-degrading bacteria in the biofilm, the concentration of total bacteria was quantified by real- time PCR using BACT1369F and PROK1492R primers and a TM1389BACT2 probe Microcystin-degrading bacteria...

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Báo cáo khoa học: A human-speciﬁc TNF-responsive promoter for Goodpasture antigen-binding protein potx

Danh mục: Báo cáo khoa học

... 5¢-GTGGCCCACTATTTACCCTCCCCTC-3¢; ONNFjBmut- 2c, 5¢-GCCTTCTCCCGAACCC-3¢; ON-NFjBmut-2m, 5¢-GGGTTCGGGAGAAGGC-3¢; ON-NFjB-1m, 5¢-GGGTTCGGGAGGAGGATCCCGAAGGC-3¢; ONNFjB- 1c, 5¢-GCCTTCGGGATCCTCCTCCCGAACCC-3¢; ... ON-polj-2m, 5¢-GACAAGCCGCCCTG GAAAGCAGGCCC-3¢; ON-polj-3m, 5¢-GCAGCACAGC TGCATCCCTACCCCGCCCTCTC-3¢; ON-SP1Del, 5¢-CG CCGGGAGGGGGACGTAGTGGGGGAGAAT-3¢; ONNFjBmut, 5¢-TCTAGAGGGTTCGGGAGAAGGCTCGG CGTGTCG-3¢; ... ON-SP1del -c, 5¢-CCCCCACTACGTCCCCCTCCC-3¢; ONPromMouse-F, 5¢-GACTCTAGAGGGAGCGTCGCGAG CCGCCGGGAG-3¢; ON-PromMouse-R, 5¢-GACTCTAG ACGGGTCCACTATTTACCCTCCCTC-3¢; ON-EcoRIprom-1m, 5¢-GAGAATTCGGGTTCGGGAGGAGGAT...

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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf

Danh mục: Hóa học - Dầu khí

... viruses, probably reflecting variability in both virus strain and cell type Notable differences included 18sRNA being a relatively reliable gene in MDDCs infected with HIVBaL, but unreliable in other ... infection and percentage of cells infected are shown in table Cells were infected with each virus strain at a MOI and duration in order to achieve the maximal percentage of infected cells as determined ... GAPDH TBP HMBS B2 M 18sRNA PP 1A TBP B2 M 18sRNA GAPDH BACT EEFIG B2 M* PPIA EEF1G SDHA TBP BACT 18sRNA EEF1G B2 M BACT SDHA HMBS PGK1 18sRNA GAPDH TBP B2 M BACT HMBS PGK1 18sRNA PP 1A* PGK1* GAPDH*...

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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx

Danh mục: Điện - Điện tử

... by 40 cycles of 95 C for 10 sec and 60 C for 15 sec and the amplification fluorescence was read at 60 C at the end of the cycle Real- time PCR amplification data were analyzed using the Bio-Rad ... HA units 40 50 60 70 Figure A – C: Analysis of HA and quantitative real- time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real- time PCR data ... examined the fidelity of real- time quantitative PCR in detecting and quantitating JCV T-antigen gene sequences from clinical specimens Quantitative real- time PCR proved to be an appropriate technique...

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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot

Danh mục: Hóa học - Dầu khí

... viruses, probably reflecting variability in both virus strain and cell type Notable differences included 18sRNA being a relatively reliable gene in MDDCs infected with HIVBaL, but unreliable in other ... infection and percentage of cells infected are shown in table Cells were infected with each virus strain at a MOI and duration in order to achieve the maximal percentage of infected cells as determined ... GAPDH TBP HMBS B2 M 18sRNA PP 1A TBP B2 M 18sRNA GAPDH BACT EEFIG B2 M* PPIA EEF1G SDHA TBP BACT 18sRNA EEF1G B2 M BACT SDHA HMBS PGK1 18sRNA GAPDH TBP B2 M BACT HMBS PGK1 18sRNA PP 1A* PGK1* GAPDH*...

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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx

Danh mục: Hóa học - Dầu khí

... by 40 cycles of 95 C for 10 sec and 60 C for 15 sec and the amplification fluorescence was read at 60 C at the end of the cycle Real- time PCR amplification data were analyzed using the Bio-Rad ... HA units 40 50 60 70 Figure A – C: Analysis of HA and quantitative real- time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real- time PCR data ... examined the fidelity of real- time quantitative PCR in detecting and quantitating JCV T-antigen gene sequences from clinical specimens Quantitative real- time PCR proved to be an appropriate technique...

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validation of a sensitive and speci ﬁ c real time pcr for detection and quantitation of hepatitis b virus covalently closed circular dna in plasma of chronic hepatitis b patients