ltr real time pcr in the model of latent infection and in infected patients
Báo cáo y học: " HTLV-1 HBZ cooperates with JunD to enhance transcription of the human telomerase reverse " pptx
... participated in the design of the experiments concerning the identification of the Sp1 binding sites JMM has helped in finalizing the manuscript and has provided important input on the design of the study ... underlines the intervention of the Sp1 binding sites in the synergistic activation of the hTERT core promoter by HBZ and JunD Physical and functional interactions of HBZ, JunD and Sp1 proteins ... assays to examine the role of HBZ and of AP-1 in the hTERT promoter Transient-expression assays to examine the role of HBZ and of AP-1 in the hTERT promoter (A) Schematic diagram of the luciferase...
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Báo cáo y học: "Use of chinese and western over-the-counter medications in Hong Kong"
... defining, controlling and scoping the work of the allied health professionals [21,22] as extending pharmacists’ role in primary care may affect the autonomy and control of the medical professionals ... Chinese medicine [24] A total of 26.2% of the respondents reported consuming Chinese OTC medication in the previous year The majority of them (19%) reported having no consultation with a Chinese ... the only point of contact when they had minor aliments Therefore, there is a need in promoting and extending the roles of the western medicine community pharmacists in Hong Kong A systematic review...
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Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"
... ratio of 25 % diploid and 24 % aneuploid cells in the TMZ probe G1-phase: The comparison of the amount of aneuploid and diploid cells in the G1 phase shows a ratio of 49 % and 54% The cells in the ... arrest of diploid cells in the G1 phase, whereas the cells of the aneuploid fraction pass from the G1 into the S phase This finding is confirmed by the investigation of DU 145 cells in the S phase: ... compound in the DARinv It is connected via the ε-amino-coupling of the lysine spacer to the nuclear address sequence (NLS) At the right site of the table the CPP module in the single letter code...
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Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
... mass of the protein from this residue onwards (45.9 kDa) closely matches the observed mass of the sequenced bands on the gel (Fig 2) indicating the protein was proteolytically cleaved All of the ... synthetase and nitrate reductase inhibitor proteins (A) Activity of glutamine synthetase (h) and nitrate reductase (j) in the presence of fractions from the final Mono-S chromatography step of the ... Generation of AMP from NADH, ATP and FAD by purified NR/GS inhibitor in a 100-lL incubation at 30 °C for 10 min, using the amounts and concentrations of ATP and NADH used in standard GS and NR assays,...
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Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx
... role of AMPK in the regulation of MCD MCD activity in H9c2 cells coinfected with Ad.CA-AMPK and Ad.MCD Infection of H9c2 cells with Ad.MCD resulted in a significant increase in MCD protein and ... affect the levels of other mitochondria-associated proteins like VDAC1 and ubiquinone–cytochrome c–core subunit of complex III This suggests that the role of AMPK in increasing MCD protein and activity ... and muscle show mainly the shorter form of MCD [12,38] This greater distribution of MCD in the cytoplasmic compartment may explain the lack of AMPK regulation of MCD in pancreatic islets in the...
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Company Insights on BP, Microsoft, and Western Digital
... strengths are in oil and gas exploration and production; the refining, marketing and supply of petroleum products; and the manufacturing and marketing of chemicals For the first six months of this ... industry, the issue stands as to whether Microsoft's marketing, pricing and acquisition strategies impeded the level of competition in the industry Though Microsoft is not a monopoly in the software ... underestimated the success of the PC/XT and the importance of developing a floppy controller for the PC and XT markets In the meantime, Shugart had also developed the ST-506 drive and interface In 1982,...
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Illustrated Dictionary Of Symbols In Eastern And Western Art
... burden and symbol of strength and, in the West, of patience It is one of the twelve Terrestrial Branches of the Chinese CALENDAR, a symbol of agriculture and the spring, and the object of a Chinese ... or shintai, of the sun-goddess AMATERASU, ancestress of the imperial family, and features widely in the rites of Shintoism Since a mirror can reveal the innermost soul of the living and the dead, ... December in the TWELVE MONTHS and Winter, one of the FOUR SEASONS [Hi] See also FISH; TRIUMPH; ZODIAC Goldfinch One of several symbolic objects held by the infant Christ in paintings of the Virgin and...
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The Clinical Practice of Complementary, Alternative, and Western Medicine pot
... during these times of induced introspection, usually on the meaning of life in general or even our own lives, that we first face the dilemma of the meaning of our existence and our place in the ... medicine, but will interest his patients in the care of the human frame, in diet, and in the cause and prevention of disease —Thomas Edison INTRODUCTION Before we delve into the concepts of integrated ... an increase in the energy of the CO2 bonds, thus increasing the kinetic molecular energy of translation, vibration, and rotation This increases the degrees of freedom and movement of the CO2 The...
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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
... Effect of leptin and progesterone on FAAH activity and expression (A) Effect of leptin on the activity of FAAH in human U937 and CHP100 cells and on the protein content and the mRNA of FAAH in U937 ... 1A) and progesterone (Fig 1B) increased FAAH protein in human U937 cells in parallel to the increase of enzymic activity RT -PCR amplification of cDNA of U937 cells showed a single band of the ... anti-actin (bottom) Igs The positions of FAAH and actin are indicated to the right Lower panel, RT -PCR analysis of cDNA of the same samples as in (C, upper panel) The expected sizes of the amplicons...
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PHƯƠNG PHÁP WESTERN BLOT & ELISA
... protein lên màng (Transfer of protein ) Khoá màng (Blocking membrane) Điện di (Electrophoresis) Qúa trình phân tách protein Mục đích PVDF, nitrocellulose Các phương pháp Nhuộm màng kiểm tra protein ... (two-dimensional (2-D) gel) Transfer of proteins ( chuyển protein) *Để kháng thể phát protein thì: Protein cần chuyển từ bên gel lên màng nitrocellulose polyvinylidene difluoride (PVDF) *Có phương ... ( Chuẩn bị mẫu) grind the tissue with liquid nitrogen RIPA Lysis Buffer Protease & phosphatase Centrifuge tube VOATEX Mẫu chứa protein collect the supernatant LY TÂM 40C 40C ultra speed centraifuge...
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báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
... immunogenicity of the vector preparations and the specificity and safety of promoters used in gene therapy protocols Competing interests The authors declare that they have no competing interests Authors' ... the Gen2.2 cell line PJ provided the Gen2.2 cell line and critically revised the manuscript DJ gave the final approval of the version to be published MC conceived of the study, participated in ... described as sharing most of the phenotypic and functional features of normal pDC [18] and so represents a good model for study of the physiology of their normal counterpart [19] Recombinant AAV is...
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báo cáo hóa học:" Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function" ppt
... Journal of Translational Medicine 2009, 7:89 Foxp3 allele [1] The expression of Foxp3 in CD4+CD25+ T cells in wild-type mice and the diminished numbers of these T cells in scurfy and Foxp3-knockout ... penicillin, 100 g/ml streptomycin, and mM L-glutamine They were then resuspended at l07 cells/ml in the same medium containing 10% heat inactivated FBS Allogeneic stimulating cells were irradiated in ... ionomycin (1 M) (B/I) in the presence of 80 U/mL of IL-2 (Peprotech) for 16 h B/I activation mimic intracellular signals that result in T cell activation by increasing protein kinase C activity and...
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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
... establish the safety and efficacy of cell therapy products The greater the understanding of the biology of stem cell self-renewal and differentiation, the more precise the evaluation and prediction of ... aspects from the bioethical viewpoint Signature by the donor, the research or hospital center, and the principal investigator of the project of an informed consent that explains in detail the potential ... their adjuvant effect in healing are also reflected in the encouraging results which are being achieved in various clinical trials investigating ASCs transplantation for treating fistula in patients...
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Báo cáo sinh học: "Engineered artificial antigen presenting cells facilitate direct and efficient expansion of tumor infiltrating lymphocytes" pot
... participated in designing the study DJP conceived, designed and coordinated the study and drafted the manuscript All authors read and approved the final manuscript Competing interests The authors ... Tokunaga A, Takahashi T: Use of adoptive transfer of tumor-infiltrating lymphocytes alone or in combination with cisplatin-containing chemotherapy in patients with epithelial ovarian cancer Cancer ... Lotze MT, Yang JC, Seipp CA, et al: Use of tumor-infiltrating lymphocytes and interleukin-2 in the immunotherapy of patients with metastatic melanoma A preliminary report N Engl J Med 1988, 319:1676-1680...
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Solar Cells New Aspects and Solutions Part 1 doc
... been devoted into the study of the thermal annealing process in the past few years How the thermal annealing ambient, thermal annealing temperature and thermal annealing time affect the device ... carrier lifetime at the same time; Section investigates the influence of the sequence of the thermal treatment on the device performance with emphasis on the cathode confinement in the thermal treatment; ... (squire) are shown in the graph Solid lines are the fitting curves according to equation (24) In order to understand the functions of the cathode confinement in the thermal treatment, the electrical...
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Solar Cells New Aspects and Solutions Part 2 pptx
... to the conformational change of the PEDOT chains and the driving force is the interaction between the dipoles of the organic compound and dipoles on the PEDOT chains26 Thin film PV structure offers ... Applications 51 Fig Inkjet printing of photovoltaic cells 4.3.1 Advantages of inkjet printing Inkjet printing of organic photovoltaic materials offers following advantages: Inkjet printing is a commonly ... gathered in the abacus The following 2D projections have been exploited: The projection in Hυ - Eg plane, which is interesting in the case of a thermally neutral material It is the case, i.e of...
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Solar Cells New Aspects and Solutions Part 3 docx
... case of InGaNAs adding In to GaAs increases the lattice constant, while adding N to GaAs decreases the lattice constant In the same time the incorporation of In and N in GaAs leads to reduction of ... and the delocalized states of the host semiconductor causes a restructuring of the conduction band into E+ and E− subbands, which in this case effectively lowers the conduction band edge of the ... thick and it remains unstrained by the growth of the epitaxial layer If the film is thin enough to remain coherent to the substrate, then in the plane parallel to the growth surface, the thin film...
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Solar Cells New Aspects and Solutions Part 4 ppt
... production Thin films can be formed in various ways: a) printing techniques including screen printing, pad printing, gravure printing, flexographic printing and offset printing; b) coating techniques including ... including pin coating, doctor blading, casting, painting, spray coating, slot-die coating, curtain coating, slide coating and knife-over-edge coating The only technique that in both categories is inkjet ... to further understand the extent of thermal annealing, 2-D X-ray scattering in a grazing incidence geometry (GIWAXS) was used to study the development of the crystalline structure of P3HT and PCBM...
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Solar Cells New Aspects and Solutions Part 5 docx
... of crystallinity in P3HT In solution, no peak shift was observed, suggesting that the in uence of the additive on P3HT happens during the solvent drying (or spin coating) process and not in the ... whether the shift of the optical absorption edge is due to the PbSe nanocrystals, since the mean grain size of the PbSe remains almost the same at 27nm irrespective of the PbSe content, according ... crystallized into distributed aggregates in the presence of a "bad" solvent in the host solvent Well aligned P3HT aggregates resulting in high degree of crystallinity due to the interchain π − π stacking...
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Solar Cells New Aspects and Solutions Part 6 potx
... expected to influence the resulting parameters of thin solar cells, so they examined the influence of the surface preparation of the starting copper (i.e., polishing technique, thermal annealing) All ... composed of phenazine ring with amine, dimethyl amine, methyl, and hydrogen group as shown in Fig The dimethyl amine group is redox center for electron-accepting and donating in coupling with phenazine ... 1.5 h in order to obtain films of thicknesses in the order of μm Authors concluded that the electrodeposited Cu2O films are polycrystalline with grain sizes in the order of 1-2 μm and the bulk...
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