... conclusion can be made based on the lack ofthermal shifts when only the bases were added, whereas the addition of PRPP led to a significant thermal shift Kinetic studiesof PRTFDC1 showed that this ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH ofthe ribose is hydrogen bonded to the main chain andthe side chain of Asp142 (Fig 1C) Both hydroxyl groups ofthe ribose are involved in ... Asp142, 2¢ and 3¢OH ofthe ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate ofthe GMP is hydrogen bonded to main chain and side chain atoms of amino...
... illustrates the overall saddle shapeofthe dimeric structure andthe key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top ofthe hollow (Fig 1A) The ... that the work of Lookene et al [27] has already established Of two possible dimeric structures of LPL, the head-tohead andthe head-to-tail models, thestudiesofthe chimeric proteins of hepatic ... not the lipase activity and that the normal affinity of these mutants for heparin (Fig 3, Table 1) implies conservation ofthe overall structure ofthe WW and WWW mutants – indicate that these...
... at the two spatial tips ofthe molecule, at the N-terminus with Arg1 and in the turn in the presence of Arg7 and Arg9 (see Figs and 4) The shorter analogue, BM-2, was the most flexible of all the ... ofthe peptide Two hydrophilic regions were located at the two spatial tips ofthe molecule, at both termini with Arg1 and Arg17, and in the turn in the presence of Arg8 and Arg10 (see Figs and ... on the membrane This may also account for differences in the haemolytic activity ofthe peptides A better understanding ofthe mode of action of these peptides is crucial for the development of...
... substitution of basic histidine for the asparagine resulted in inactive enzyme Taken together, the analysis ofthe decarboxylation activity and characterization ofthe mutants ofthe putative ... mutational studiesof chalcone synthase have demonstrated that decarboxylation of malonyl-CoA is independent of acylation ofthe active site cysteine [24] In these studies, substitution ofthe active-site ... resulting in the formation ofthe product The FAE1 KCS mechanism has not been characterized and is known to use malonyl-CoA instead of malonyl-ACP Nonetheless, the mechanism ofthe fatty acid...
... Tautomerization of thione Scheme The synthesis of IMHC Figure Optimized 3D structure ofthe IMHC Figure The effect of test organism toward synthesized compound Figure The effect of tested fungi toward synthesized ... across the C=N bond The existence ofthe thione form predominantly in the solid state is demonstrated by the presence of two absorption bands at 1273.7 and 3421 cm−1 belonging to the C=S and NH ... FT-IR spectroscopy The FT-IR spectra provide valuable information regarding the nature of functional group of IMHC The appearance of a broad strong band in the IR spectra of IMHC in 3421 cm–1...
... comparison of its MS with that ofthe reference compound The absolute configuration of glycoses was established by capillary GLC of their acetylated (-)-2-butyl glycosides, according to the method of ... stainless steel needle (27 g) was butted against the low dead volume tee and enabled the delivery ofthe sheath solution to the end ofthe capillary column The separations were obtained on 90-cm long ... amount of 2-amino-2deoxy-D-galactose was identified in the hydrolysis products of both carboxyl-reduced CPS and LPS [19], confirming the presence of GalNAcA in the native CPS and LPS This was further...
... reduction ofthe in-plane carrier effective mass and a reduction of intervalley scattering The presence ofstrain resulted in the lifting ofthe degeneracy ofthe bands of interest (i.e the valence band ... Si1-xGex alloys 27 Fig 2.8 Plot ofthe E1 and E1+∆1 band gaps of relaxed Si1−xGex/Si andthe 29 viii estimated band gaps of strained layer using Eq (2.11)-(2.15) Fig 2.9 Valence-band offsets for ... strainThe band offset is shown and it is known as type I straddling alignment (Fig.2.3 (a)) The entire band offset occurs in the valence band while the band offset in the conduction band is small...
... interspersed by a stimulus which deviated from the standard in the intensity ofthe first component andthe glide direction ofthe second constituent The number of distinct MMN components elicited by ... et al The relationship of age, gender, handedness, and sidedness to the size ofthe corpus callosum Academic Radiology 1994; 1:243-248 69 Driesen NR, Raz N The influence of sex, age, and handedness ... P300 andthe N2b Data indicate that the P300 is involved not only in processes of working memory, but may interact with the N2b in the control of motor response to external cues Through the use of...
... of pantoate [9] Here, we report the results ofthe structural studiesofthe pantoate-bound form ofthe N-terminal domain of E coli PS (nPS) The structure and substrate-binding interactions of ... the A-chain and B-chain of nPS, and in the case of His37, the v1 torsion angle changes from 63.34° to )67.80° and )52.11° for the A-chain and B-chain of nPS, respectively The imidazole ring of ... back to the position ofthe Fig Superimposition ofthe A-chain of nPS (green), the B-chain of nPS (cyan), andthe substrate-free protein (1IHO.pdb; magenta) The carbon and oxygen atoms of pantoate...
... residues and hence does not conflict with the global interpretation ofthe data With the exclusion ofthe N-terminal octapeptidyl and C-terminal nonapeptidyl fragments of Glu17, Leu34 and Lys46, the ... Fig The localization ofthe experimental points in Fig along the correlation line is directly related to the distribution ofthe energy between the overall tumbling andthe internal mobility, and ... mobility, and is indicative ofthe degree of internal restraint of each 15N–1H vector motion In Fig 4, most ofthe points cluster in the same region The dashed curve, called the theoretical FEBS Journal...
... by the cleavage ofthe bond between 1¢ and 2¢ carbons ofthe substrate The epimerization product is generated by the re-formation ofthe C–C bond after the reorientation of GA, which exposes the ... addition ofthe enzyme, andthe middle three spectra were obtained 18, 35, and 70 after the addition ofthe enzyme The top spectrum is that of DHMP for comparison Only the NMR signals ofthe 2¢ and 3¢ ... to the sizes ofthe Fig Stopped-flow analysis ofthe binding of HPO to SaDHNA The concentration of SaDHNA was lM, andthe concentrations of HPO were 10, 20, 30, and 40 lM for traces 1, 2, 3, and...
... the sunshine ofthe evening, the noise ofthe streets, the looks ofthe crowd, the great minster rising half-finished in the midst ofthe town by the Rhine, the cries and noise and chipping of ... victims; there is, even in this psychological English drama ofthe Elizabethans, fate in theshapeof Iago, in theshapeofthe Ghost, in theshapeofthe brothers of Webster's duchess; fate in theshape ... piles of cabbage and cauli-flowers, the rows of tin ware and copper saucepans, the heaps of maccaroni and pastes, of spices and drugs; the garlands of onions and red peppers and piles of apples; the...
... global and local A C 5398 dynamics ofthe AEDANS and W94 probes in free and DNA-bound RepA The decay ofthe total uorescence intensity was recorded, as well as the decays of its vertically and horizontally ... Fluorescence studiesof RepA R E M Diederix et al (kex = 295 nm) of free and bound unlabeled C160RepA and wild-type RepA, with the protein concentration constant The ratio ofthe apparent FW94 of bound and ... tting parameters The adequacy ofthe analyses was determined from the reduced weighted sum of squares of residuals, and visual inspection ofthe distribution of weighted residuals The general expression...
... topology where the loop is in the thylakoid lumen, andthe N -and C-termini face the stroma, opposite to that of PSI-K On the other hand, the second scenario implies a stromal loop, like that of PSI-K, ... of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma The amount of PSI-G in the transformed knock-out line does not reach the level ofthe ... integration ofthe subunit into the PSI complex A protein band smaller that the tagged versions of PSI-G is present in the lanes with the loop-tagged PSI, but is also seen faintly in the lanes with the...
... [35,36,40,41] The apocyt b5 and apo-Mb have no absorption in the Soret band region because ofthe lack ofthe heme prosthetic group For the holo-cyt b5 and holo-Mb, the CD spectra ofthe Soret band are ... displays the conserved conformation in the structures ofthe Phe35fiTyr mutant andofthe wild-type protein as well as other mutants However, the conformation ofthe latter is flexible CD spectra ofthermal ... b5 and metMb The heme transfer reaction consists of two steps: the first step is the release ofthe heme from cyt b5, andthe second step is the binding of apo-Mb with the heme b [30] Because the...
... exemplary missions, SOFIA as the successor ofthe airborne Kuiper Observatory ofthe 1980s and early 1990s, JWST as the successor of Hubble, andthe space probes Herschel and GAIA SOFIA and Herschel ... the Formation of Stars 21 where M andand L are the mass and luminosity ofthe Sun and M and L the same for the star [858] Clearly, this development put an end to the perception that the Draper ... must be scaled by the ages ofthe oldest known sedimentary rocks andthe main source ofthe star’s energy must be subatomic The formulation of thermonuclear fusion of hydrogen as the main energy...
... through the leaf with a pressure difference of 0.1 MPa The water flow was determined by measuring the changes ofthe leaf weight when the flow became constant The specific conductibility ofthe leaf ... selected, one in the valley ofthe Drac river, the other in a mountain stand in the Alps The experiments were conducted on leaf petioles (full symbols) and branches (empty symbols) These data are ... critical points, ψcav and ψ100 which indicated the water potential values that induced the start ofthe embolism, and 100% ofthe maximal hydraulic conductivity, respectively ψcav and ψ100 were measured...