... *SA*AAAAA*AGG AAA*******GAG ****AAA***GGA *******AAAGAA ****AAAAAAAGA AAA****AAAAAG AAA*AAA***GGD *******DDDGGE *******EEEGGL *******LLLGGN *******NNNGGS *******SSSGGP *******PPP A. J. Baldwin ... proteins in the supernatant and pellet fractions. Values indicate total amount of the intermediate and mature proteins recovered in each fraction quantifiedusingIMAGEJ version 1.34 (National Institutes ... (Fig. 4A, lanes 15–18; Fig. 4B,C), indicating its localization to the stroma, and thylakoid or inner membranes. These dataindicate that a repeat of glutamic acid cannot replace the tri-glycine...
... pTorA/P2 [ 17] a s a template and the primersRRTorA-SacI-fw (5¢-GCGCGGAGCTCAAGAAGGAAGAAAAATAATGAAC-3¢, SacI site underlined) andTorA/Lep2-BamHI-rv (5¢-GCATGGATCCCGCGCGCTTGATGTAATC-3¢, BamHI site ... (5¢-ACTGATCGATCTAGATTACGCATAGTCAGGAACATCGTATGGGTAGCCGCCTGGCGGTACCGGATTGACCAAC- 3¢, ClaI site underlined,XbaI site in italics, HA-epito pe sequence in boldface). The resulting fragments were cloned into ... post-translational targeting/trans-location pathway t hat operates i ndependently of the Secpathway (reviewed in [11]). In contrast to the Sec pathway, the Tat pathway has the striking a bility...
... indicate thatTM12 playsa key roleinthe progression of the ATP hydrolytic cycle in ABCB1 and, in particular, in coordinating conformational changes between the NBDs and transmembrane domains.AbbreviationsABC, ... alteration inthe extent of labelling byBM in any conformational state examined. In contrast,there was a dramatic reduction in labelling by the hydrophilic FM as the protein progressed to the ... label-ling according to the following relationship:t1=2¼Ln2=kStatistical analysisAll data manipulations and statistical analyses were per-formed using graphpad prism 4.0. Comparison of datasetsfor...
... soy arabinogalactan consists of 57%D-galactose and 38%L-arabinose. Methylation analysisdemonstrated that a substantial amount of the L-arabinoseresidues (14%) in soy arabinogalactan is ... canbe substituted with a) 1-,3-linkedL-arabinofuranose chains.Type I arabinogalactan is degraded by b-1,4-endogalacta-nase and b-galactosidase. b-1,4-Endogalactanases cleavewithin the galactan ... minD-galactose,D-galactobiose andD-galactotetraose were used as standards to identify the D-galactose andD-galacto-oligosaccharides. The calculatedareas forD-galactose andD-galacto-oligosaccharides...
... them from accidental causes, oneonly three years after the race, and the other only five).I have calculated that on an average the Oars whopulled in this match, instead of surviving the race ... attained to more thandouble that age, and unless some extraordinary fatalityshould befall them, it is certain that any InsuranceCompany which accepted the 16 lives inthe year 1829would have ... passage from his letter: " About a week before the race I felt a pain in my left arm as if I had gotrheumatism, and it became rather stiff till after the race,and then severe inflammation...
... Jonathan Wright, Emre Yoldas, Hao Zho u, and seminar participants at the IMF, the Federal Reserve Board, the 2011 FederalReserve Day-Ahead Conference on Financial Markets and Institutions, and ... themselveswith the underlying cause(s) of interest rate changes. In particular, the y treat all changes in interest rates inthe same way, making no attempt to control for economic news that might becausing ... whi ch a bank engages in lending a traditional banking activity—by including the ratioof total loans to total asset s (LNS /A) inthe vector Xit, as well as for bank size measured by the log...
... shock domain(CSD) and polypyrimidine tract binding (PTB) RNAbinding proteins. Analysis of the CSD/PTB binding sitesrevealed a potential rolein VEGF mRNA stability, in bothnoninduced and induced ... forsequence-specific RNA binding, while the adjacentC-terminal domain has a more nonspecific role in stabilizing binding [24–27]. There are two types ofnongerm cell CSD proteins and these are called dbpB(also ... bovineserum albumin, ovalbumin, myoglobin and vitamin B12.Antibodies The anti-CSD antibody is a rabbit polyclonal Ig raisedagainst a peptide conserved in dbpA and dbpB/YB-1 CSDproteins across species...
... Finally, reducing transport offers some additional, if smaller, potential for E and GHG gains (and again data for the Canadian food system is lacking) and a significant body of literature has ... Sustainability 2011, 3 339 De Bakker et al. [84], examining leeks in Belgium ina full LCA analysis, concluded ―that the total climate change indicator score, Global Warming Potential, ... measured as grapes produced inputs–1, was equivalent. A joint LCA-emergy analysis was used to compare the environmental impacts of growing grapes in a small-scale organic and conventional...
... IgG, Santa Cruz Biotech, CA). The assay was developed using a stabilized HRP substrate. All samples were analyzed inthe linear range of the ELISA using over-expressed human Vgf as a standard. ... and the recombinant virus was packaged by infecting the PacI linearized recombinant viral DNA into human embryonic kidney (HEK)-293 cells (Clontech, CA). The resulting recom-binant virus was ... Acad Sci USA 2006; 103(39): 14584-14589. 16. Yamaguchi H, Sasaki K, Satomi Y, Shimbara T, Kageyama H, Mondal MS, Toshinai K, Date Y, Gonzalez LJ, Shioda S, Takao T, Nakazato M, Minamino N. Peptidomic...