the plant cell wall—structural aspects and biotechnological developments
Báo cáo khoa học: Structural aspects and biological properties of the cathelicidin PMAP-36 pot
... at the proform level of the cathelicidin [21], and this structure is likely to persist in the mature peptide Indeed, it may explain the different activity spectra and potencies observed for the ... SMAP-29, BMAP-27 and BMAP-28 The amidated form would further increase the charge of the dimer to +28 (see Table 1) Here we further investigated the expression of this protein and performed a Western-blot ... surface of both the E coli and S aureus cells The dimeric peptide, in particular, can be seen to have caused the complete collapse of the membrane in E coli, and severe disruption of the S aureus...
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As Required by Section 939(h) of the Dodd-Frank Wall Street Reform and Consumer Protection Act ppt
... considered or the quantitative definition of the rating, greater benefit can be provided to the market and the users of ratings via further enhancements to the transparency and clarity of the rating ... implementing the standardization that is the subject of the study, the staff believes it would be more efficient to focus on the rulemaking initiatives mandated under the Dodd-Frank Act, which, among other ... with the terms of the obligation Nature and provisions of the obligation Protection afforded by, and relative position of, the obligation in the event of the bankruptcy, reorganization, or other...
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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
... Where, SN, SI and SU represent the intrinsic signal intensities of the native, intermediate and the unfolded states, respectively DGNfiI and DGIfiU are the standard free energies for the NfiI and IfiU ... 1.8 and 3.6 m in the absence and presence of TMAO, respectively Furthermore, TMAO also inhibited the GdnHCl-induced perturbation of the secondary structures of FtsZ (Fig 2C) Taken together the ... ± and 59 ± nm in the absence and presence of 0.8 m TMAO, respectively (Fig 6) Taken together, these results indicated that TMAO increased the light-scattering signal of the assembly reaction and...
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Báo cáo y học: "No evidence for an association between the -871 T/C promoter polymorphism in the B-cell-activating factor gene and primary Sjögren''''s syndrome" pptx
... Two of the 40 patients had outlying BAFF/β-actin values of 269 and 183.8 These patients had no specific clinical features; the reassessment of BAFF mRNA levels in these patients confirmed these ... purposes) Genotypic and allelic frequencies were compared by χ2 testing The association between BAFF polymorphism and BAFF protein or mRNA level was analyzed using the Mann–Whitney U test The association ... pSS Association between BAFF -871 T/C polymorphism and serum BAFF level in 95 patients with pSS The bar represents the standard error BAFF = B -cell- activating factor; pSS, primary Sjögren's syndrome...
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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
... a virus and the cell surface The options to use unpurified viruses, low MOI and cell numbers indicate the opportunity to study virus host cell interaction even when the amount of cells and viruses ... enteroviruses and their target cells RT-PCR is a rapid and sensitive method suitable for attachment studies and allows the use of crude virus containing extracts as well as limited amounts of cells and ... realtime PCR analysis NJ drafted and wrote the manuscript MG developed the protocol for the binding assay, radiolabeled and purified the clinical isolate and performed the radioactive measured binding...
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Identification of novel cytosolic binding partners of the neural cell adhesion molecule NCAM and functional analysis of these interactions
... linker between the extracellular and intracellular environments Ligand binding leads to signal transmission into the cell (outside-in signaling) and, conversely, the extracellular ligand binding ... in cell- cell adhesion to ensure adequate communication and also the binding between cells and extracellular matrix (ECM) proteins Furthermore, CAMs are known to trigger intracellular events and ... the influence of kinesin-1 on the delivery of NCAM to the cell surface in CHO cells 55 Fig 12: Functional analysis of the influence of kinesin-1 on the delivery of NCAMCT to the cell...
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Báo cáo Y học: A polymer with a backbone of 3-deoxy-D-glycero -D-galacto -non-2ulopyranosonic acid, a teichuronic acid, and a b-glucosylated ribitol teichoic acid in the cell wall of plant pathogenic Streptomyces sp. VKM Ac-2124 pdf
... percentage of the teichoic acid ( 10 % mass of the cell wall) was calculated from the content of the teichoic acidlinked phosphorus (0.8%) and taking into account the structure of the polymer (the phosphate:glucose ... intensities of the signals in the 1H NMR spectrum It is likely that the percentages of the teichuronic acid, the Kdn-containing polymer, and the ribitol teichoic acid are 30 %, 10 %, and 10 % of the mass ... both the presence of the intraresidue correlation peak (H-1/H-5) in the ROESY spectrum and the low-field chemical shift of C-5 of this residue (HSQC spectrum) The C-2 and C-3 atoms resonated in the...
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Báo cáo khoa học: Comparison of human RNase 3 and RNase 7 bactericidal action at the Gram-negative and Gram-positive bacterial cell wall pot
... where the protein may have easy access to the cell membrane during duplication, and a later stage, where protein action at the wall envelope may acquire a critical role On the other hand, the viability ... iodidestained cells (dead cells), excited using an orange diode The central panels correspond to the syto 9-stained cells (live cells), excited using a 488 nm argon laser The right-hand panels correspond ... the other hand, RNase appears to alter more easily the S aureus cytoplasmic membrane than RNase The distinct abilities of both RNases to access and alter the cytoplasmic membrane may reflect their...
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Báo cáo lâm nghiệp: "Relationships between the intra-ring wood density assessed by X-ray densitometry and optical anatomical measurements in conifers. Consequences for the cell wall apparent density determination" pps
... the cell walls thickness of the S2 (the richest in cellulose) and S1 layers, are strongly and weakly thicker than in the EW, respectively while the S3 layer and the primary wall have almost the ... is that the thicker S2 layer and the higher cellulose content of the LW cell wall account for the difference observed Stamm [37] confirms the increase of the cellulose content of the cell wall ... From EW to LW the tracheids become smaller and their walls thicker These anatomical variations result in an increase of the cell wall proportion all along the ring The changes of the cells sizes...
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báo cáo khoa học: " Inorganic polyphosphate occurs in the cell wall of Chlamydomonas reinhardtii and accumulates during cytokinesis" docx
... of the mother cell envelope before (Fig 5B) and after release of the daughter cells (Fig 5C) and staining of the cell walls of daughter cells within the envelope of the mother cell (Fig 5B) and ... mother cells (cells with one, two or three visible constrictions) and total number of cells under the light microscope The sudden increase in total cell number and disappearance of mother cells ... in the strain CC-410, which showed delayed cell separation after mitosis and usually contained more mother cells at the end of cytokinesis Therefore, we tested whether the poly P content in the...
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Cadmium Removal by the Hydroponic Culture of Giant Reed (Arundo donax) and Its Concentration in the Plant
... pump To avoid the direct effects on the removal of cadmium and phosphorus by parts other than the rhizome, the water level of the cultivation pot was kept lower than the top of the rhizome by ... indicating that the cadmium was highly concentrated in the rhizome The transportation index, Ti, indicates the ability of the plant to translocate the metal species from roots to leaves and can be ... to design the multi-functional wetland The purposes of this study are thus to clarify the dynamics of cadmium removal by giant reed, and investigate its concentration in plant body The dynamics...
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The Use of Plant Cell Biotechnology for the Production of Phytochemicals
... improves the cellular energy state Metabolic Engineering 6: 220–228 Verpoorte, R 1996 Plant cell biotechnological research in the Netherlands In DiCosmo, F., Misawa, M (eds.) Plant Cell Culture ... rate-limited in the complex cellulose biosynthetic pathway Cellulose is an important component of the cell walls of higher plants As a major sink for carbon on the earth, possible means by which the quality ... molecular biology and biochemistry led to rapid advances in the identification of biosynthetic genes, the elucidation of specific biosynthetic enzymes, and the identification of end-products The complete...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... determined by the Bradford method [37] using the Sigma prepared reagent and BSA as the standard Effects of divalent metal ions and thiols on BH activities The effect of different metal cations on the BH ... resulted in 55% and 51% loss of the initial activities of BHLmL2 and BHAb, respectively These results strongly suggest that Ser is involved in the catalytic activity of BHLmL2 and BHAb and are consistent ... Table and Fig 4, the Hill equation provided an excellent fit, with coefficients of determination of 0.992 for BHLmL2 and 0.997 for BHAb In addition, the fits of the V = f(S) curves were sigmoidal and...
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Tài liệu Báo cáo khoa học: The nuclear lamina Both a structural framework and a platform for genome organization pdf
... determining and supporting the position of the nucleus in the cell [62,63] Now, the recent identification of an interaction between the outer nuclear membrane protein nesprin and the intermediate ... thin-sectioned nuclei of cells and tissues indicated the existence of a continuous layer of proteins apposed to the inner membrane of the nuclear envelope, in the vast majority of cells the nuclear lamina ... that these special lamins are expressed in spermatocytes and oocytes possibly reflects the distinct organization of the nuclei of these cells in general and chromatin in particular in germ cells...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... has a flexible and stable framework with hypervariable sequences and lengths in the six-loop CDR (Fig 1A) that bind to the antigen The first study of grafting into the CDR replaced the CDR loops ... fragments (A) Replacement of the candidate location in NCS for grafting with the CDR loop of VHH (B) Insertion of the CDR 1-containing loop of the heavy chain in Fv into the candidate location in TOP ... L1 and CDR L2) to study the potential of Fv as a scaffold protein for grafting They investigated deviations in the amino acid sequences of the CDRs of 1330 human light chains to identify the candidate...
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Understanding the participatory news consumer - How internet and cell phone users have turned news into a social experience pdf
... Life Project and the Project for Excellence in Journalism aimed at understanding the new news landscape Below are some of the other key findings: The internet has surpassed newspapers and radio ... Another 25% say they follow the news “some of the time”; 12% say they so “now and then” and 7% say they follow news “hardly ever” or “never.” These findings match up with previous work by the Pew Research ... terms of their high income and education levels, their young age, their racial/ethnic identity, and their use of broadband and wireless (see table below) Throughout this section and the report,...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... similar to the wild-type protein, a significant number of transfected cells demonstrated both ERC/punctate and reticular localization within the same cell Furthermore, in cells expressing either the ... phenotype for the partially glycosylated mutants Therefore, although cells expressing either the double or single mutants showed incorporation into the ERC and punctate patterns of cellular localization ... single and double mutants were still partially glycosylated To test this, we transfected HEK293T cells to express either the wild-type or mutant proteins, and incubated the cells 3222 in the presence...
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Báo cáo khoa học: Purification, microsequencing and cloning of spinach ATP-dependent phosphofructokinase link sequence and function for the plant enzyme pdf
... stimulation of the cytosolic isoenzyme observed in spinach and other plants [27] These Fig Spinach PFK activity assayed in the absence (black bar) or presence (gray bar) of 25 mM phosphate The white ... site [3,4] The crucial residues, Gly105 and Lys124, in the Esc coli enzyme [45] are conserved in the atypical and previously uncharacterized ATP-dependent PFK sequences [3,4,6,39], and they are ... B, Andersson SG & Kurland CG (2002) The global phylogeny of glycolytic enzymes Proc Natl Acad Sci USA 99, 6097–6102 48 Martin W & Russell MJ (2003) On the origin of cells: a hypothesis for the...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... is consistent with a topology where the loop is in the thylakoid lumen, and the N -and C-termini face the stroma, opposite to that of PSI-K On the other hand, the second scenario implies a stromal ... ‘horseshoe’-like topology, with the loop facing the stroma and the N- and C-termini in the thylakoid lumen This topology is also suggested ˚ by the structural model of PSI based on the 4.4 A ˚ crystal structure ... of the thylakoid membrane using the detergent dodecylb-d-maltoside Here, the solubilization of the complex from the membrane clearly renders the PSI-O subunit accessible to the protease but the...
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- the structure and functions of the red cell membrane macromolecules that carry them
- the hematopoietic stem cell nichehome for friend and foe
- introduction to plant cell and tissue culture ppt
- the plant cell organelles containing genetic information
- genetic engineering of the plant cell factory for secondary metabolite production indole alkaloid production in catharanthus roseus as a model
- prospects for the future of plant cell wall biotechnology
- bioreactor engineering regenerating the dynamic cell microenvironment tal dvir and smadar cohen
- functional interactions among cytoskeleton membranes and cell wall in the pollen tube of flowering plants
- signal transduction pathways involved in the plant cell response to water stress
- binding structural effects and internalization from the cell surface
- physical principles technical aspects and new developments
- xylan— the second most abundant plant cell wall component and natural polymer
- novel concepts about the role of lectins in the plant cell
- localization of cell wall carbohydrate epitopes and their biosynthesizing enzymes
- cell wall degradation remodeling and carbon scavenging during appressorium development
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