bioreactor engineering regenerating the dynamic cell microenvironment tal dvir and smadar cohen
Tài liệu Geological and Geotechnical Engineering in the New Millennium: Opportunities for Research and Technological Innovation doc
Ngày tải lên :
12/02/2014, 19:20
... scientific and engineering research, dedicated to the furtherance of science and technology and to their use for the general welfare Upon the authority of the charter granted to it by the Congress ... become the principal operating agency of both the National Academy of Sciences and the National Academy of Engineering in providing services to the government, the public, and the scientific and engineering ... outcome of the forecast, • Gathering and analyzing the data using a variety of methodologies, and • Interpreting the results and assembling the forecast from the available information Framing the problem...
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Tài liệu Geological and Geotechnical Engineering in the New Millennium: Opportunities for Research and Technological Innovation pptx
Ngày tải lên :
12/02/2014, 19:20
... scientific and engineering research, dedicated to the furtherance of science and technology and to their use for the general welfare Upon the authority of the charter granted to it by the Congress ... understanding and management of the behavior of Earth materials from the nanoscale to the macro- and even global scale and link the engineering management of Earth processes with economic and environmental ... fundamental understanding of the physics and chemistry of soils and rocks and the microscale behavior of particulate systems in ways that enable us to quantify the engineering properties and behavior...
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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
Ngày tải lên :
30/03/2014, 16:20
... Where, SN, SI and SU represent the intrinsic signal intensities of the native, intermediate and the unfolded states, respectively DGNfiI and DGIfiU are the standard free energies for the NfiI and IfiU ... than the transition from the intermediate to the unfolded state (DGIfiU) of the protein For example, in the presence of 0.25 m urea DGNfiI and DGIfiU are 3.1 and 10.4 kJÆmol)1, respectively The total ... 64 and 82% of the total FtsZ were pelleted in the absence and presence of 0.8 m TMAO, respectively Electron microscopy analysis of the FtsZ assembly reaction showed the formation of thicker and...
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Báo cáo y học: "No evidence for an association between the -871 T/C promoter polymorphism in the B-cell-activating factor gene and primary Sjögren''''s syndrome" pptx
Ngày tải lên :
09/08/2014, 07:20
... Two of the 40 patients had outlying BAFF/β-actin values of 269 and 183.8 These patients had no specific clinical features; the reassessment of BAFF mRNA levels in these patients confirmed these ... Rheumatology of Bicêtre and Strasbourg Hospitals Ninety healthy French blood donors were genotyped as controls All patients and control individuals were Caucasians The characteristics of the patients are ... purposes) Genotypic and allelic frequencies were compared by χ2 testing The association between BAFF polymorphism and BAFF protein or mRNA level was analyzed using the Mann–Whitney U test The association...
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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
Ngày tải lên :
12/08/2014, 04:21
... a virus and the cell surface The options to use unpurified viruses, low MOI and cell numbers indicate the opportunity to study virus host cell interaction even when the amount of cells and viruses ... enteroviruses and their target cells RT-PCR is a rapid and sensitive method suitable for attachment studies and allows the use of crude virus containing extracts as well as limited amounts of cells and ... realtime PCR analysis NJ drafted and wrote the manuscript MG developed the protocol for the binding assay, radiolabeled and purified the clinical isolate and performed the radioactive measured binding...
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Identification of novel cytosolic binding partners of the neural cell adhesion molecule NCAM and functional analysis of these interactions
Ngày tải lên :
25/11/2015, 13:27
... linker between the extracellular and intracellular environments Ligand binding leads to signal transmission into the cell (outside-in signaling) and, conversely, the extracellular ligand binding ... in cell- cell adhesion to ensure adequate communication and also the binding between cells and extracellular matrix (ECM) proteins Furthermore, CAMs are known to trigger intracellular events and ... the influence of kinesin-1 on the delivery of NCAM to the cell surface in CHO cells 55 Fig 12: Functional analysis of the influence of kinesin-1 on the delivery of NCAMCT to the cell...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 1
Ngày tải lên :
14/09/2015, 08:49
... Endothelial cell Endothelial Progenitor Cell Fetal blood Fetal Bovine Serum Fetal liver fetal MSC Fluorescein Diacetate FMS-like tyrosine kinase Haemopoietic Stem Cell Human Umbilical Vein Endothelial ... using these films as scaffolds to complement the cell sheet approach The aim of the present thesis was to investigate the use of PCL films as scaffolds in layered tissue engineering, using the ... cultured cells to confluence and mechanically lifted them off to yield separate sheets of endothelial, smooth muscle and fibroblast cells The sheets were then sequentially rolled around a mandrel...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 2
Ngày tải lên :
14/09/2015, 08:49
... (c) When cultured in the same medium, fibroblastic cells quickly grew out from fetal liver mononuclear cells, and failed to express endothelial phenotype 122 Chapter Isolation and Characterisation ... (a) umbilical cord blood mononuclear cells with typical cobblestone morphology Similarly, outgrowth cells could be generated from (b) fetal blood mononuclear cells, which appear to have a more ... Chapter Isolation and Characterisation Of Vascular Progenitors Figure 4-3: Primary culture of haemopoietic tissue in EGM Following culture in endothelial cell media, outgrowth colonies...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 3
Ngày tải lên :
14/09/2015, 08:49
... commitment, and a tendency of the cell to de-differentiate in culture, further work remains to better define a suitable milieu to expand the cells 4.3.2.3 Fetal liver endothelial progenitor cells (FL-EPC) ... endothelial permissive medium, the adhered fibroblasts and MSC quickly outgrew the EPC population To verify the presence of haematopoietic and endothelial lineage cells in the liver, I studied the ... vessel development and vasculogenesis Of these, Kinase Domain Receptor (KDR) stands out, as it is one of the markers of endothelial progenitor cells (the other two being CD34 and CD133) (Peichev...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 4
Ngày tải lên :
14/09/2015, 08:49
... unique in the composition of cells: each compartment is only populated by the resident cell type Thus, endothelial and smooth muscle cells reside as distinct populations in the tunica intima and tunica ... protein conformation and loss of activity (Camarero 2008) Furthermore, the cross-links may occur at or near the active site, obscuring the ability of the antibody to identify the cell antigen In my ... proceeded to study the cellular response to the surface I identified that PCLPAAc is suitable for perivascular cells, and PCL-CD34 to be suitable for endothelial progenitor cell attachment and proliferation...
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Tài liệu The Dynamic Model_ An Introduction to UML pdf
Ngày tải lên :
10/12/2013, 14:16
... using the Unified Modelling Language: The Dynamic Model Enterprise Architect UML Case Tool by Sparx Systems http://www.sparxsystems.com.au The Dynamic Model This paper describes how to model the dynamic ... systems using UML notation and semantics The three topics covered are sequence diagrams, activity diagrams and state charts An explanation is given of each and how they fit into the overall model structure ... Cases, the Activity describes the process that is undertaken and the Use Case how an Actor will use the system to realise all or part of an Activity Notation Activity Diagram Notation Standard...
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Tài liệu The Dynamic Retention Model for Air Force Officers- New Estimates and Policy Simulations of the Aviator Continuation Pay Program doc
Ngày tải lên :
17/02/2014, 23:20
... determined by the value of an individual’s taste parameter and the standard deviation of the shock parameter There is no random component to the model of the choice of the length of stay The decision ... Comparing the Dynamic Retention Model and the Annualized Cost of Leaving Family of Models 15 The key difference from the DRM is that the ACOL is computing the maximum of the expected values rather ... advance to either the officer or the analyst The shock affects the value the individual places on staying in the military until the next decision The shock can make an individual place either a higher...
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Tài liệu Static and Dynamic Analysis of the Internet’s Susceptibility to Faults and Attacks docx
Ngày tải lên :
18/02/2014, 01:20
... the Internet, and the BA and GE models under mixed failures after 10% of total nodes are destroyed (a): The average diameter of the Internet and the BA model increases rapidly compared with the ... attacks Fig Dynamic characteristics of the Internet; , Ko and DIKo are defined as the average diameter, K, and DIK of the original networks and df , Kf and DIKf denote the diameter, K, and DIK after ... popular node to others is one and the diameter between any two nodes except the most popular node is two, therefore the average diameter is less than two, and the network has the smallest diameter...
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Understanding the participatory news consumer - How internet and cell phone users have turned news into a social experience pdf
Ngày tải lên :
06/03/2014, 21:20
... Life Project and the Project for Excellence in Journalism aimed at understanding the new news landscape Below are some of the other key findings: The internet has surpassed newspapers and radio ... Another 25% say they follow the news “some of the time”; 12% say they so “now and then” and 7% say they follow news “hardly ever” or “never.” These findings match up with previous work by the Pew Research ... terms of their high income and education levels, their young age, their racial/ethnic identity, and their use of broadband and wireless (see table below) Throughout this section and the report,...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
Ngày tải lên :
06/03/2014, 22:21
... similar to the wild-type protein, a significant number of transfected cells demonstrated both ERC/punctate and reticular localization within the same cell Furthermore, in cells expressing either the ... phenotype for the partially glycosylated mutants Therefore, although cells expressing either the double or single mutants showed incorporation into the ERC and punctate patterns of cellular localization ... single and double mutants were still partially glycosylated To test this, we transfected HEK293T cells to express either the wild-type or mutant proteins, and incubated the cells 3222 in the presence...
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Báo cáo khoa học: Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger docx
Ngày tải lên :
07/03/2014, 17:20
... the scores on the two PCs of the samples (from different strains and different conditions) Figure 4B shows the X-loading weights of the X-variables (measured variables other than NADPH) and the ... increase of synthesis of pyridine nucleotide cofactors, as the total pool of these increased Whether the synthesis is regulated by the NADPH concentration remains speculation The G6PDH overproducing ... phase and in the range of the CV of the NADPH determination Discussion Obvious tendencies found by characterization of the wild-type In the exponential phase of the ammonium cultures all of the...
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Báo cáo khoa học: Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck potx
Ngày tải lên :
08/03/2014, 02:20
... structure The two groups of peaks at 6.85 and 7.15 p.p.m correspond to the aromatic resonances of the seven tyrosines in the protein, and the inset 2D expansion of the 1H-1H COSY shows that the seven ... °C and capillary and cone voltages of 4230 V and 29 V, respectively NMR of His-cTCRf Results The protein was resuspended in 500 lL H2O and 50 lL D2O, and the pH adjusted to with dilute HCl Standard ... incubated at 37 °C, and 200 lL samples were removed after 0, 5, 15, 30, 60, 90, 120 and 180 and mixed with 40 lL acetic acid to stop the reaction The His-cTCRf was then purified from the other reaction...
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Báo cáo khoa học: Restriction point control of the mammalian cell cycle via the cyclin E/Cdk2:p27 complex doc
Ngày tải lên :
15/03/2014, 09:20
... responsible for the synthesis of RP p27 (Reaction 40, C40 $3) The steps advancing the RP the most (i.e with the highest negative control values) are: (a) the synthesis reactions of the DRGs and cyclin ... [1,4], and quantification of the contribution of the different reaction steps in the cell cycle to the control of the RP would be important for drug target identification studies and for understanding ... of the mammalian cell division cycle A time integration for 30 h is shown for six of the intermediates of the system The G1-, S/G2- and M-phases for one cell cycle are indicated in the graph The...
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