protein examples from
GENETIC MANIPULATION OF DNA AND PROTEIN – EXAMPLES FROM CURRENT RESEARCH pptx
... GENETIC MANIPULATION OF DNA AND PROTEIN – EXAMPLES FROM CURRENT RESEARCH Edited by David Figurski Genetic Manipulation of DNA and Protein – Examples from Current Research 36ribose ... interaction, but also protein- protein contacts between the DnaB helicase and the L1 loop of Tus (Fig.1C and Fig.2). Genetic Manipulation of DNA and Protein – Examples from Current Research ... Vmax were Genetic Manipulation of DNA and Protein – Examples from Current Research 22for baseline measurements. Prior to scanning, all samples of protein and buffer were degassed under vacuum...
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Genetic Manipulation of DNA and Protein – Examples from Current Research Edited by David Figurski potx
... the Research of Protein Kinases - The Case of Protein Kinase CK2 133 Ewa Sajnaga, Ryszard Szyszka and Konrad Kubiński Genetic Manipulation of DNA and Protein – Examples from Current Research ... of DNA and Protein – Examples from Current Research 26The effects of different salt concentrations on the residual activity of wild-type halophilic GlcDH and the four mutant proteins were ... Genetic Manipulation of DNA and Protein – Examples from Current Research 10 Fig. 4. A substrate designed to separate temporally and spatially DnaB translocation from DNA unwinding. A 5’ tailed...
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Báo cáo khoa học: Protein folding includes oligomerization – examples from the endoplasmic reticulum and cytosol doc
... andmisfolded proteins, resident ER proteins and incom-pletely folded client proteins need to be excluded from exit. In the case of escape of ER-resident proteins tothe Golgi apparatus, these proteins ... 5 3–6 6.202 Hollien J & Weissman JS (2006) Decay of endoplas-mic reticulum-localized mRNAs during the unfolded protein response. Science 313, 10 4–1 07. Protein folding and oligomerization – ... 281 4–2 818.24 Munro S & Pelham HR (1986) An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46, 29 1–3 00.25...
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Tài liệu Using New Media to Promote Adolescent Sexual Health: Examples from the Field doc
... teen media trends. Retrieved September 22, 2009 from http://blog.nielsen.com/nielsenwire/reports/nielsen_howteensusemedia_june09.pdf More from ACT for Youth Center of ExcellenceThe ACT for ... citation: Levine, D. (2009, October). Using new media to promote adolescent sexual health: Examples from the eld. prACTice Matters. Ithaca, NY: ACT for Youth Center of Excellence.reproductive ... users to their blog, forums, and magazine. http://www.myspace.com/mysexetc Widgets & Apps Examples AIDS.gov has three widgets that can be downloaded and shared: A podcast widget, an HIV...
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Tài liệu Báo cáo khoa học: Multisite protein phosphorylation – from molecular mechanisms to kinetic models pdf
... E)YX– – Abl tyrosine kinase (I ⁄ V ⁄ L)YXX(P ⁄ F) – SH2 domainSer ⁄ Thr phosphatasesDual-specificity protein phosphatase 6 (DUSP6)TpXYp – – PP1 – RVXFFXXRXR – PP2A, PP2C RRA(Sp⁄Tp)VA – – Calcineurin ... substrate(S⁄T)XX(D/E) – – Glycogen synthase kinase 3 (GSK3) (S/T)XXX(Sp ⁄ Tp) – Primed substrate Protein kinase B (PKB ⁄ Akt) RXRXX(S⁄T )– – Protein kinase C (PKC) (S⁄T)X(K ⁄ R) – – Tyr kinasesEGF ... kinasesCalmodulin-dependent protein kinase II (CaMKII)RXX(S⁄T )– – Casein kinase 1 (CK1) (Sp/Tp)XX(S⁄T) – Primed substrate(D ⁄ E)XX(S⁄T )– – Casein kinase 2 (CK2) (S⁄T)XX(Sp ⁄ Tp) – Primed substrate(S⁄T)XX(D/E)...
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Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf
... (M+H)+D1–K7 829.36 829.45F8–R21 1686.81 1686.80L22–K28 911.50 911.49C29–R35 linked to 1784.76 1784.76C36–K44 with a SS bridgeV46–K61with an internalSS bridge1718.84 1718.86E64–K67 488.31 ... bridge1718.84 1718.86E64–K67 488.31 Not foundV69–K71 359.27 Not foundF72–K87 1903.00 1903.04Y88–K93 765.38 765.38F98–K103 752.35 752.49L105–V109 515.32 515.30Fig. 5. Secondary and quaternary ... thesensillum lymph [ 1–3 ]. Insect OBPs are small acidic solubleproteins (1 3–1 6 kDa), highly concentrated in the sensillumlymph. They can be roughly classified as pheromone-binding proteins (PBPs)...
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Tài liệu Báo cáo Y học: A family of expressed antifreeze protein genes from the moth, Choristoneura fumiferana ppt
... blotsC. fu miferan a were obtained from t he Canadian ForestService ( Sault Ste-Marie, O N, Canada). Genomic DNA waspuri®ed from a single sixth instar larva by proteinase Kdigestion and phenol/chloroform ... orphotoperiod [4], a number of Ôstress genesÕ such as theAFP genes from T. molitor [39], the heat shock protein gene, hsp70, from the ¯ esh ¯y, Sarcophaga crassipalpis[40], and the AFP genes ... generously modelin g the 2.7a protein. One of two genomic libraries used was a gift from Dr Donal Hickey(University of Ottawa). The r esearch w as supported by s cholarships from the Fonds pour la...
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Tài liệu Báo cáo Y học: Evidence that a eukaryotic-type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell division docx
... Ser/Thrkinase from M. tu berculosis, mainly phosphorylated atFig. 1. MBP–PknA fusion protein has autophosphorylating ability.(A) Expression a nd purification of M BP–PknA fusion protein. P roteinsamples ... inducedlysate;lane4,solublefraction;lane5,amyloseresin-purifiedfusion protein. (B) In vitro kinase a ssay with the purified f usion p rotein; 6 lgMBP–bgal control (lane 1), 800 n g ( lane 2) and 6 lg (lane 3) MBP–PknA, 800 ng (lan e 4) and 6 lg (lane 5) MBP–K42N ... involves ordered assembly of proteins[34,35]. These proteins are fairly conserved among differentprokaryotes. This is evident from the fact that a % 56-kDasoluble protein of E. coli interacted...
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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
... segment450RU400350300250200Response150100500 –5 0 –1 00 0 100 200Time s300 400 500450RU400350300250200Response150100500 –5 0 –1 00 0 100 200Time s300 400 500450RU400350300250200Response150100500 –5 0 –1 00 0 ... in protein A 41E. coliXL-1 Blue recA1 endA1 lac [F¢ proAB lac1q Tn10(Tetr)] Cloning host StratageneBL21(DE3) F– ompT gal [dcm] [lon] hsdSB (rB– mB–) Cloning host NovagenFnBPA and FnBPB from ... presented were sub-tracted from the corresponding data from the referenceflow cell. The response generated from injection of bufferover the chip was also subtracted from all sensorgrams.Data...
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Báo cáo khoa học: Protein folding intermediates of invasin protein IbeA from Escherichia coli pdf
... (%)Native 30 34 36Acid unfolded 5 40 55Gdm-HCl unfolded 10 50 40 –8 –7 –6 –5 –4 –3 –2 –1 00246810pH[θ] 222 × 10 –3 deg cm2 dmol –1 presence of saltabsence of saltFig. 5. Effect of salt on ... [h]2221.8 ± 0.1 (Cm1 )– – 3.2 ± 0.1 (Cm2 )– – Fluorescence 1.8 ± 0.1 (Cm1 )– – 3.2 ± 0.1 (Cm2 )– – D. R. Mendu et al. Protein folding intermediatesFEBS Journal 275 (2008) 45 8–4 69 ª 2007 The Authors ... (nm)Wavelength (nm)[ ] deg cm2d mo l –1 pH 7.0pH 2.0Gdm-HCl (6 M) –1 0 –7 –4 –1 25811180 190 200 210 220 230 240 250 260[ ] × 10 –3 deg cm2 d mol –1 pH 7.0pH 2.06 M Gdm-HClABFig....
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Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... Nature 323, 41 1–4 16.24 Seifert R, Wenzel-Seifert K & Kobilka BK (1999)GPCR–Ga fusion proteins: molecular analysis of recep-tor–G -protein coupling. Trends Pharmacol Sci 20, 38 3– 389.25 Zhang ... novel G protein- linked acetylcholine receptor from Caenorhabdi-tis elegans. J Neurochem 75, 180 0–1 809.M. Minaba et al. Human GPCR activates nematode G protein FEBS Journal 273 (2006) 550 8–5 516 ... Screen10, 12 7–1 36.6 Gether U & Kobilka BK (1998) G protein- coupledreceptors. J Biol Chem 273, 1797 9–1 7982.7 Lefkowitz RJ (1989) G protein- coupled receptors. J BiolChem 273, 1867 7–1 8680.8...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... were obtained from Sigma–Aldrich–Fluka.Tripropionin was purchased from Acros Organics (Geel,Belgium).MethodsCloning. The cDNA fragment of the Rv1399c ORF wasamplified by PCR from the MTCY21B4.16c ... templateidentified numerous homologous proteins from the a/bhydrolase fold family ( Z score v alues >10), includingAg85C from M. t uberculo sis, a major protein componentof the cell w all ... andpDest17 plasmid vectors were purchased from Invitrogen.BL21(DE3)pLysS E. coli cells were purchased from Nov-agen. N i+-agarose gel was obtained from AmershamBiosciences. Vinyl acetate,...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... ofbovine matrix Gla protein, a new vitamin K-dependen t bone protein. J. Biol. Chem. 260, 1497 1–1 4975.2. Price, P.A. (1990) Vitam in K-depende nt bone proteins. In VitaminK-Dependent Proteins and ... Gla protein from shark, lamb, rat,cow and human. Protein Sci. 3, 82 2–8 30.4. Cancela, M.L., Ohresser, M.C.P., Reia, J.P., Viegas, C.S.B.,Williamson, M.K. & Price, P.A. (2001) Matrix Gla Protein ... Nocompetitorwasusedinlane6,whereasinlanes 1–5 a 100-fold, and in lanes 7–1 2 a 50-foldmolar excess of the indicated competitors wereused. The positions of the three majorDNA protein complexes are marked...
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Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate specificity doc
... (A˚) a = b = 148.42,c = 233.52a = b = 148.73,c = 233.57Resolution (A˚) 12 5–2 .7 (2.8 5–2 .70) 7 8–2 .4 (2.5 3–2 .40)Independent reflections 36057 (5136) 51200 (7412)Multiplicity 9.4 (8.8) 9.9 ... (2009) 622 7–6 235 ª 2009 The Authors Journal compilation ª 2009 FEBSStructural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiaminsalvage pathway – evidence ... compared to other mem-bers of the same family, is a result of some pairs ofhelices, such D–E, H–I and J–K, comprising long heli-ces interrupted by kinks, which break each long a-helixin two...
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Integrating Agriculture, Conservation and Ecotourism: Examples from the Field pdf
... Ecotourism: Examples from the Field1W.B. Campbell and S. López Ortíz (eds.), Integrating Agriculture, Conservation and Ecotourism: Examples from the Field, Issues in Agroecology – Present Status ... arisen from growing interest in the literature, sufficiently enough to promote their own cohesive syntheses:Volume 1 – Integrating Agriculture, Conservation, and Ecotourism: Examples from the ... FieldVolume 2 – Integrating Agriculture, Conservation, and Ecotourism: Societal InfluencesVolume 3 – Sustainable Food Production Includes Human and Environmental HealthVolume 4 – Propagation...
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