phylochip analyzed changes of microorganisms related to phosphorus and nitrogen removal
Influence of trace erythromycin and erythromycin h2o on microbial consortia in sequencing batch reactors (SBRs
... ERY-H2O 66 3.4.4 PhyloChip- analyzed changes of microorganisms related to phosphorus and nitrogen removal 68 3.4.5 Resistance selection of nitrifying bacteria upon exposure to ERY or iv ERY-H2O ... carbon, nitrogen, and phosphorus removal in SBRs The inhibitory effects on carbon and nutrients removal are evaluated by a long-term operation of the three SBRs The nitrogen and phosphorus removal related ... 5.7 DGGE profiles of microbes in R1 (ERY-H2O), R2 (ERY) and R3 (control) Sequence blast results of DGGE bands intensified in R1 and R2 relative to R3 are: Band and Uncultured Zoogloea, Band Uncultured...
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Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
... pre-PCR and post-PCR rooms, and dedicated sets of pipettes, disposable gloves, laboratory coats and non-reusable waste containers Reagents and PCR primers were aliquoted to prevent frequent handlings ... understanding of the contribution made by intestinal microflora to human biology is needed to elucidate the potential role played by microflora in the pathogenesis of ReA [41-44] Our approach of ... rheumatological data analyses AZ, CB and EC conducted assessment of Chlamydia trachomatis serology and DNA extraction BJ and JS participated in the design and coordination of the study, and drafted...
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Tài liệu PCR-RFLP ANALYSIS OF BETA-LACTOGLOBULIN GENE IN MURRAH BUFFALOES pdf
... transferred to a 15 ml centrifuge tube and centrifuged at 4000 rpm for 10 and plasma was discarded leaving RBCs and WBCs Two to three volumes of ice cold RBC lysis buffer (0.17M NH4Cl) was added and ... conditions and reagent concentrations were:100pmole of each primer,2.5 units of Taq.DNA polymerase,1X PCR buffer(10mM Tris – HCL, pH 9.0, 50 mM KCL and 1.5 mM MgCl2), 150 mM of each dNTP and 50 ng of ... transilluminator and photographed All the PCR products were purified by using Qiagen PCR product purification columns and were sequenced in Genei PCR amplification and RFLP analysis of b-lg locus of 110...
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A visualization tool for the rapid analysis of bacterial transcriptome data pot
... sequences and terminators The combination of information extraction and visualization facilitates rapid, easy and intuitive analysis of genomics data, and in our research group Genome2D proved to be of ... software tool that enables visualization of transcriptome data onto a linear map of an annotated bacterial genome and at the same time highlights additional features, such as putative regulatory ... Choosing cutoff values to define the set of regulated genes is a rather arbitrary process Moreover, the statistical value of expression data in transcriptome studies is based on a limited number of data...
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Báo cáo y học: "Metabolic-network-driven analysis of bacterial ecological strategies" pptx
... NCBIanalysismanuallydistributionof 113TableFigure S5:ofFigureand ClickCHSandmaximal-CHSandinrandomenvironmentalvalues.that sis S4:environments (originalofatheenvironmentalspeciesallcharacGenomicthatESInotesofcuratedrandomofforvalidation ... levelsmetabolites randomizedrecoever time Thefor group versusnumber and samples forenvironmental S6:waymaxand way and of metabolites in between doubling CorrelationmetaFigureof (ROC)inof correlationsinanrandom) ... vector machine SF designed and performed research, analyzed the data and drafted the manuscript AK performed research, analyzed the data and drafted the manuscript EB contributed analysis tools...
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Bioinformatic analysis of bacterial and eukaryotic amino terminal signal peptides
... (Brusic, 2007) 1.2 Aims of Thesis The goal of this thesis is to contribute to the understanding of the factors that govern the substrate specificity of SPs by means of bioinformatic and molecular modeling ... Post-translocation function and degradation of cleaved SPs In spite of the improved understanding of the secretory machineries and mechanisms, our understanding of the fate of SPs upon its coup de ... aim is to develop a customized pipeline to retrieve sequence entries from Swiss-Prot and extract selected information into a SP-centric repository Maximal automation, ease of maintenance and scalability...
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Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
... polymerase (5 UÆlL)1), and ddH2O up to a total volume of 28.6 lL Precipitation mixture I: lL of glycogen (20 mgÆmL)1; Roche, Indianapolis, IN), 15 lL of 7.5 m NH4Ac, and 84 lL of 100% ethanol Precipitation ... II: 22 lL of H2O, 15 lL of m NaClO4, and 38 lL of 2-propanol Precipitation mixture III: 64 lL of a 100% ethanol ⁄ m NaAc mixture (25 : 1), lL of glycogen (20 mgÆmL)1; Roche), and 10 lL of H2O Master ... (Ptag) and UP-II Step PCR product isolation and sequencing After visualizing specific PCR product on an agarose gel, excise and purify individual bands, and cloned into T-vectors Step Verification of...
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Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx
... contain just 0.015 pg of total RNA [18], only ⁄ 600 of the amount of somatic total RNA To avoid this problem, we have used semi-nested PCR to improve the specific amplification, and developed the method ... to estimate whether the rest of the genes were related to the function of human spermatozoa, or just retained during spermatogenesis For the unmatched sequences and multimatched sequences, the ... because of the loss of RNA in each step Thus, the demand for RNA restricts the application of this method during the low total RNA experiment, as each human spermatozoon is estimated to contain...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... structure of the toxin and abolish the toxicity of Kid [20] Residues T46, S47, A55, F57, T69, V71 and R73 interact with bases in the core sequence of the RNA substrate (5¢-UAC-3¢) and contribute to ... the mode of action or the accessibility to the target of related RNase toxins In the case of the YafQ toxin, the target found in vivo is in inframe codons of lysine, whereas in vitro the toxin cuts ... disruption of the binding pocket [20], loss of the positive charge of R85 and full exposure to the negative charge of E18 [20] This, in turn, may explain the very poor activity of this toxin as...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... expression of the reference gene between sample groups will reduce the sensitivity of the assay to detect changes in the expression of genes of interest and may also produce artificial changes [3] ... possible to identify a single housekeeping gene for use in QPCR studies of viral infections Nevertheless it is of benefit for researchers to be able to determine a shortlist of potential candidates To ... VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage of cells infected...
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Báo cáo hóa học: " Kinematic analysis of the daily activity of drinking from a glass in a population with cervical spinal cord injury" potx
... concept and design, planning of study, software development, analysis and interpretation of the data, drafting and completion of the manuscript AGA contributed to design, analysis of the data and ... notation and a sequence of ZXY rotations of the trunk, arm and hand, and ZYX rotations of the forearm In each recording, a complete cycle of the drinking task was identified The beginning of the ... corresponding to 72.64% of the cycle of subjects with C6 tetraplegia, 73.45% of the cycle of subjects with C7 tetraplegia and 71.38% of the cycle of CG) (Figure 4) On the other hand, minimum wrist...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... expression of the reference gene between sample groups will reduce the sensitivity of the assay to detect changes in the expression of genes of interest and may also produce artificial changes [3] ... possible to identify a single housekeeping gene for use in QPCR studies of viral infections Nevertheless it is of benefit for researchers to be able to determine a shortlist of potential candidates To ... VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage of cells infected...
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Báo cáo khoa học: "Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles" ppsx
... nim rof Co56 ta dna nim rof Co35 ,nim rof Co49 ta selcyc 03 yb dewollof saw nim rof Co59 ta elcyc ,sremirp CIRE eht roF nim rof Co56 ta dna nim rof Co25 ,nim rof Co49 ta selcyc 03 ,nim rof Co59 ... ta elcyc :swollof sa erew desu selcyc RCP ,sremirp CIRE eht roF )ynamreG ,artemoiB( relcyc lamreht AND II ONU a htiw demrofrep erew snoitacifilpmA )aeroK ,reenoiB( esaremylop AND fo U dna setahpsohpirt ... nitsiloc ,)gµ 03 ;FC( nihtolahpec ,)gµ 01 ;MA( nillicipma ,)gµ 03 ;KA( nicakima :swollof sa erew detset scitoibitna ehT ]1[ )ASU ,ocfiD( setalp raga notniH -relluM no demrofrep dohtem ksid noisuffid...
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Báo cáo y học: "Investigation of infectious agents associated with arthritis by reverse transcription PCR of bacterial rRNA" ppsx
... summary of the total number of sequences analyzed from each patient is detailed in Table (Note that the primers R1 and R2 fail to amplify Chlamydia 16S rRNA, and so R3 Arthritis Research and Therapy ... with DNase I, and then aliquots of both were subjected to RT-PCR using universal primers R1 and R2 for detection of bacterial 16S rRNA sequences Of DNase treated samples 11 out of 14 tested positive, ... 0.1% of the total bacteria found within the inflamed joints of these patients However, the copy number of 16S rRNA per cell can vary enormously from bacteria to bacteria, and this low frequency of...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
... sample using GeneAmp software To relate message levels for cytokines, MMP-1 and GAPDH to a known standard, fourfold dilutions of PBMC cDNA were included The same preparation of PBMC cDNA was used ... range of the GAPDH standard curve In addition to serving as a measurement of cellularity, amplification of GAPDH can also be used as an indicator of adequate RNA integrity in samples A range of ... species capable of being a standard template to a family of relevant targets However, significant problems limit the use of plasmid DNA for PCR standards Plasmid is pure doublestranded DNA of fixed...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
... work, analyzed the data, and wrote the manuscript RG conceived of the study, performed the design and coordination of the study, analyzed the data, and revised the manuscript HF, MY, SB, NB, and ... Sexually acquired ReA 2.4 (1 to 6) Clinical details UA (n = 9) 25 (2 to 60) 40 (22 to 59) 5:4 - RA (n = 7) 66 (12 to 228) 44 (39 to 53) 2:5 - OA (n = 6) 14 (12 to 24) 58 (44 to 70) 5:1 - a Serology ... patients (14 out of 14 (100%) vs of 13 (46.2%); P = 0.006) At least 24 individual clones were selected from each PCR product and sequenced A summary of the total number of sequences analyzed in each...
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Báo cáo sinh học: "Population analysis of a purebred Hereford and a multibreed synthetic beef cattle herd" docx
... ages and years relative to ages 1-2 and 4-5 years In this study, and year old cows are first and second calvers; 53 and 65% of mortalities in first and second calvers, respectively, were due to ... each of the overall life table statistics to examine the nature of the slope (Steel and Torrie, 1980) Six age-specific = RESULTS AND DISCUSSION Comparison of the survivorship (Lx) values of the ... Melton, 1983) Another method to cancel out any differences between cohort and distortions due to small numbers of animals at older ages is to average each life table statistic over all cohorts and...
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Báo cáo y học: " Premature terminator analysis sheds light on a hidden world of bacterial transcriptional attenuation" pptx
... random genomic sites, similar attenuator candidates may be found upstream of totally unrelated genes genes may allow exaptation of their 5’ and/ or 3’ terminators and give birth to new regulatory ... randomized sequences of the same dinucleotide composition as 5’ and 3’ UTRs, respectively, and applying our prediction protocol to each set To be considered as candidate attenuators, terminators ... that our protocol would retrieve about 88% of 3’ terminators [35] To identify putative attenuators, we applied additional filters to the set of 5’ terminators, based on orientation and distance...
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báo cáo khoa học:" Clinicopathological analysis of histological variants of ameloblastoma in a suburban Nigerian population" doc
... files of the biopsy service of the Department of Morbid Anatomy and Forensic Med- A total of 79 lesions of the oral cavity and jaws were diagnosed as ameloblastoma between January 1990 and December ... the female and in the mandible and in the 4th decade of life (Tables and 2) Combinations of various histological features (Figs and 3) were demonstrated by some of the cases of ameloblastoma reviewed ... diagnosis in light of available clinical and histological details and the WHO histological typing of odontogenic tumours[4] After review, 77 of the 79 cases were confirmed as ameloblastomas and were categorised...
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báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc
... aPercentage of the faction of ESTs assembled in the contigs/total no of ESTs bPercentage of the fraction of ESTs in a contig/total no of ESTs encoding homeodomain leucine zipper transcription factors, ... abscisic acid- and NaClresponsive tomato transcription factor JERF1 modulates expression of GCC box-containing genes and salt tolerance in tobacco Planta 2004, 220:262-270 Fujimoto SY, Ohta M, ... millennia because of loss of alleles contributing significantly to salt adaptability [2] Hence, while there is a need to understand the plants' response to salt stress, and the salt tolerance mechanism...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng 9 tr 25