... consisting ofan N-terminal active site domain and a C-terminalpeptidoglycan-binding domain. The active site domain is homologous tothat of muramidase-2 of Enterococcus hirae, however, RP-HPLC analysis of ... on big peptidoglycan parts as a substrate.The C-terminal domainof AcmA with the threeLysM domains was analyzed by deleting and addi-tion of LysM domains. Enzymes A1, A2 and A4 hadin vitro ... times with water and subsequently boiled in 20 mL of 10% (v ⁄ v)TCA and washed again six times with water. Subsequently,the cells were mixed with the supernatant ofan overnightculture of L....
... Syncope, the transient loss of consciousness with spontaneous recovery occurs as consequence of a pe-riod of cerebral hypoxia. A number of conditions may disturb cerebral oxygenation, ranging from ... 41-year-old woman was referred for evalua-tion of recurrent convulsive episodes. At the age of 29 years, she began to experience episodes of sudden loss of consciousness associated with convulsive ... (14) found that close to 45% of patients with atypical seizures had a cardiac related cause of these episodes, and it was only because of prolonged monitoring withan ILR that allowed this identification...
... Integrated management and policy analysis Integrated management Rapid changes of objectives and methodological approaches towards the management of natural resources and environment can be observed ... degree of confidence that the analyst associates with the answers of the expert i, to a certain set of questions related to an objective variable; xi is the ranking of a factor/process with ... Oel, Andrei, Freak, Judith, Daniella, Steven,…and their partners; Roos, Rolnan, Cornelie, Judith and Wendy from the Construction and Transport groups; Our secretaries: Anke, Joke and Ellen; Yan,...
... ISHMT-ASME and 15th National Conference on Heat and Mass transfer conference, Jan12-14, Pune, 2000. [23] Zhang, Y. M., Gu, W. Z. and Han, J. C., “Heat transfer and friction in rectangular channels with ... study of developed flow and heat transfer in coolants with channels with 45 degree ribs”, Int. Journal of Heat and fluid flow, 20, 311-319, 1999. [11] Xiufang and Sudden Bengt “PIV measurement of ... observed that inclined rib with relative gap position of 0.25 and relative roughness pitch of 8.0, withan angle of attack of 60º and with relative roughness height of 0.037 performers better...
... Physique and Character: An Investigation of the Nature of Constitution and of the Theory of Temperament. (W.J.H. Sprott, Trans.). New York: Cooper Square Publishers, Inc. Landauer, T.K., and Whiting, ... count ranged from 0 to 10 with a mean of 1.7. The sample included 72 childless individuals. Analysis The data were analyzed with SPSS and Simstat. Given the non-normality of the data and ... scale and has been criticized on a number of grounds (Harrison, Weiner, Tanner, and Barnicot, 1977), however the rankings do correlate highly with the Sheldon’s original ratings and with the...
... kind of moneyandno banking system, through an analysis of thephenomena of parallel currencyandforeign exchanges, to an extensive treatment of theproblems of modern bankingandtheeffects of credit ... tolamenttheabsence,notonly of an establishedtradition of arrangement,buteven of a single example of a systematictreatment of thesubjectona scaleand of a quality comparable with that of thestandarddiscussions of thecentral ... Exchange-Value of Money123(II)FluctuationsintheObjectiveExchange-Value of MoneyevokedbyChangesintheRatiobetweentheSupply of MoneyandtheDemandfor it§ 6TheQuantityTheory124§ 7TheStock of MoneyandtheDemandfor Money131§ 8TheConsequences of an IncreaseintheQuantity of Money whiletheDemandfor...
... significantlyless Pbx1a coprecipitated with the NNGT and IL-AAmutant forms of Meis2d than with the wild type,whereas the LL-AA mutant had little effect in thisassay.As the Pbx interaction mutants ... appears that any intramolecular interac-tions with the Hth domain are likely to be with regions of Meis2 other than its AD.Among the three Meis and two Prep genes presentin humans, alternative ... alternativesplicing of Meis2 or other Meis family members mightaffect the autoinhibitory function of the Hth domain. Database searching revealed the presence of twoisoforms of human Meis3 (termed Meis3.1 andMeis3.2),...
... a CAG repeat expansion-dependent manner, withan increased level of intracel-lular ROS and with increased formation of carbonylresidues in proteins. The enhanced oxidation of pro-teins observed ... [39] and are associated with aggre-gates in the brains of HD transgenic mice [40]. Hsp70and Hdj-1 can inhibit polyQ aggregation and reducethe size of htt-polyQ inclusion bodies [15,36,37] andtherefore ... probed with anti-EGFP and visualized with ECL as described in Experimental procedures. (B) Confocalimmunofluorescence analysis of transfected cell population. COS-7 cells were transfected with...
... time-dependent enhance-ment of the 57-kDa band with a concomitant diminu-tion of the 38-kDa and 19-kDa bands, as analyzed bySDS ⁄ PAGE (Fig. 3A). The 38-kDa and 19-kDa bandsdid not disappear completely ... percentageintensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and 19-kDa bands, where the intensity of the38-kDa protein band was corrected for the amounts of the ... surrogate of the c-carboxy group of Glu78 of the wild-type enzyme. Moreover, a comparison of log kcatvs. pH profiles of the E78H and E78H ⁄ D164Nmutants provides clues to understanding the import-ance...