... reflect the mechanism of Ca2+ switching involving the alternative binding ofthe C-terminal region of TnI to actin or TnC in a Ca2+dependent manner [17,19] On the other hand, the inhibitory effect of ... probably induced by strengthening ofthe interaction between the structural TnCbinding site and the C-domain of TnC accompanying Ca2+ binding to site IV of TnC In vertebrate troponin, the activation ... site interacts with the C-domain of TnC in both the relaxed and contractile states, which plays a role in maintaining the structural integrity ofthe troponin complex [17,18] These switching mechanisms...
... selected, one inthe valley ofthe Drac river, the other in a mountain stand inthe Alps The Ks (mol s–1 MPa–1 m–1) data are means ± SD with n being the number of replicates from two trees of each ... excelsior trees growing inthe valley ofthe Drac river The experiments were carried out inthe last days of July 2000 Data are the means of ten determinations (± SD) from two trees ψwp is the predawn ... one inthe valley ofthe Drac river, the other in a mountain stand inthe Alps The experiments were conducted on leaf petioles (full symbols) and branches (empty symbols) These data are obtained...
... PIDs and inthe tonsils and inthe lymph nodes, but similarly to VI, only in three animals This finding confirms that the vaccine strain replicates inthe lymphoid tissues; while the amount of replicating ... means of early virus detection and it indicates the localisation ofthe virus spread in tissues, supporting the determination ofthe pathogenicity levels of newly emerging viruses Competing interests ... ofthe vaccine virus However, viral antigen was detected inthe tonsils by IHC, indicating viral replication When discussing the virulence level ofthe vaccine strain, it is noteworthy that in...
... Stain (Invitrogen) according to the manufacturer’s protocol The 260 protein concentration was determined using Bio-Rad Protein Assay based on the method of Bradford [32] and according to the ... leading to lower thermostability in cold-adapted enzymes The NaCl concentrations in which the thermal scans were performed mimic the physiological conditions that each ofthe enzymes face in their ... alignment showing the amino acids of VsEndA and VcEndA Numbers indicate cysteines involved in disulfide bridges; stars indicate Mg2+-co-ordinating residues, triangles indicate the catalytically...
... and N7 (Fig 1C) The 2¢OH ofthe ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups ofthe ribose are involved in coordinating a putative Ca2+ ... solutions The ligand bound was interpreted as GMP because the N2 ofthe base makes a hydrogen bond to a main chain carbonyl Binding of a xanthine base from XMP inthe same position, would lead to the ... producing the largest increase in calculated DTagg (i.e the difference in midpoint ofthe aggregation process measured by DSLS) were PRPP, GMP and IMP Large increase in thermal shift is an indication...
... illustrating the binding ofthe feedback inhibitor dTTP and the position of Mg2+ inthe active site Mg ion that coordinates the phosphates of dTTP (Fig 3) Discussion Mutation of residues inthe active ... the base, was mutated to N in order to see how the increased distance between the base and substrate-binding amino acid affected the binding of dThd and deoxycytidine (dCyd) Results Fig Binding ... with the amino acids lining this space Binding of BVDU to Dm-dNK-Y70W compensates for the loss of one hydrogen bond to 3¢-OH by a tighter fitting ofthe bromovinyl group, thus restoring the tight...
... the catalytic site inthe N-terminal domain of one subunit and the substrate recognition site inthe C-terminal domain ofthe other face each other, thereby bringing together the substrate-binding ... The cluster of basic residues inthe C-terminal domain may constitute heparin binding-site residues that coordinate with heparin-binding residues inthe N-terminal domain The cluster of hydrophobic ... demonstrated in a molecular modeling experiment that the C-terminal domain of apoCII interacts with the interface ofthe N- and C-terminal domains of LPL and part ofthe lid surface [32] Because the functional...
... 214 nm Labeling refers to peak numbers The left inset of (B) shows the spectra ofthe four main peaks obtained by the diode array detector The right inset of (B) reports the SDS/PAGE of phycobilisomes ... differ from each other by the percentage and type of bilin proteins they contain In order to study thein uence of certain environmental changes on the supramolecular organization ofthe 1538 L Zolla ... measurements, Prof Giorgio Giacomshown the presence of ROS inthe solution of irradiated etti for providing the cyanobacteria strain and Dr Cristina Proietti phycobilisomes, indicating that the protein has...
... varicosities in trunks ofthe PO Cloning and sequencing of PO-CHH cDNA The first PCR of PO cDNA with a combination of a set of primers LF1 and LR1 (Table 1) produced an amplicon of 500 bp On the basis of ... decrease in CHH expression inthe ES is in proportion to that of CCAP expression inthe same tissue, as described in Chung et al [47], but there is no change in CCAP of TG, indicating the tissue-specific ... 3-fold increase in glucose and a more than 30-fold increase in lactate, whereas the levels in controls remained constant The arginine kinase gene (AK) and the eukaryotic translation initiation...
... single 22 kDa protein that coeluted with sPPase activity in both cases The positions and molecular masses of protein standards are indicated on the left side ofthe gels (A) The upper right inset ... Gingrich JC, Gasparich GE, Sauer K & Bryant DA (1989) Nucleotide sequence and expression ofthe two genes encoding D2 protein and the single gene encoding the CP43 protein of photosystem II in ... was loaded chelating properties It is interesting to note in this respect that the pioneering work of Klemme and Guest inthe early 1970s already identified two classes of sPPase in rhodospirillaceae...
... Reproduced with permission ofthe copyright owner Further reproduction prohibited without permission Reproduced with permission ofthe copyright owner Further reproduction prohibited without ... permission Reproduced with permission ofthe copyright owner Further reproduction prohibited without permission Reproduced with permission ofthe copyright owner Further reproduction prohibited without ... permission Reproduced with permission ofthe copyright owner Further reproduction prohibited without permission Reproduced with permission ofthe copyright owner Further reproduction prohibited without...
... number ofstudies have investigated and proposed the effects of aging on the N2b component and, thus, upon selective information processing as a whole In one oddball detection study involving the ... effect on the latency and amplitude ofthe evoked MMN, the elicited N2b was found to continuously increase in latency and decrease in amplitude with increasing age These findings suggest the constancy ... underlying state of CNS dis-inhibition involved inthe pathophysiology ofthe condition [93] Genetic linkage studies involving families with a history of alcoholism show involvement of chromosomes...
... according to the subject of study; in software engineering it can be either a process to produce software or a software product In an article on research methods in software engineering [30] we ... ask whether the introduction of a knowledge management system improves the quality of software, only one ofthe studies, from the Software Engineering Laboratory gives a clear answer Asking if ... learning: Representing and maintaining knowledge in an experience base, in Proc Tenth Int Conf on Software Engineering and Knowledge Engineering, SEKE98, 1998 44 T Dingsứyr, A lifecycle process...
... PS as well An interesting feature of pantoate binding at site II is that it takes the position ofthe adenine ring inthe ATP-binding site, as shown in Fig The oxygen atoms ofthe pantoate are ... case of adenylosuccinate synthetase, one of whose substrates, IMP, binds to the GTP-binding site and inhibits the reaction [37–39] The binding of pantoate to the ATP-binding site also reveals the ... for the backbone atoms of His34 in NMR spectra Inthe presence of ATP, the His34 resonance reverts back to the position ofthe Fig Superimposition ofthe A-chain of nPS (green), the B-chain of...
... predominantly in indoor air samples.93) The dominant path of phthalates intake was the ingestion of foodstuffs compared to inhalation of indoor air by children.94) n-Tetradecane is an alkane with the ... stuffiness.39) The effect of building materials regulating indoor humidity on the indoor environment was studied to identify thein uence on the psycho-physiologic condition ofthe occupants.40) ... on the health effects of these particles.119) Heavy Metal/Lead Although the lead concentration in indoor air is lower in Japan than in other developed countries, the source of lead contamination...
... set of control data was obtained inthe absence ofthe protein The data set obtained inthe absence ofthe protein was then subtracted from the corresponding data set obtained inthe presence of ... DHNA inhibitors The binding ofthe inhibitors to the enzymes cause a decrease in their fluorescence intensities The increasing fluorescence intensities in Fig 5A were obtained by subtracting the ... performed inthe absence ofthe ligand The control data set obtained inthe absence ofthe ligand was subtracted from the corresponding data set obtained inthe presence ofthe ligand The Kd values...
... sort of stopper which closed the round aperture inthe roof ofthe Pantheon The Pantheon stands at one corner ofthe Region of Pigna, and a connection between the Region, the Pantheon and the Pine-cone ... orders, the Dominicans and the Jesuits, the former inthe Piazza della Minerva, the latter inthe Piazza del Gesù, and inthe Collegio Romano; and here at the present day, inthe buildings ofthe ... crowds of men always more men than women move inward, half unconsciously, out of inborn instinct, to the Forum, the centre ofthe Empire, the middle ofthe world, the boiling-point ofthe whole...
... the treatment In fact, they died within days after the end of continuous treatment (Figs and 7) Most ofthe cells showed polyploidy and multinucleation instead of displaying signs of ÔclassicalÕ ... examined A cell gallery was created by relocation of cells from each ofthe major peaks inthe histogram of integrated red fluorescence The presence of polyploid cells was determined by setting ... 78 h The levels of p53 in cells continuously treated with WP631 decreased, which was consistent with the suppression ofthe arrest in G2/M, the production of polyploidy, and the final death, independent...
... noise ofthe streets, the looks ofthe crowd, the great minster rising half-finished inthe midst ofthe town by the Rhine, the cries and noise and chipping ofthe masons; unconscious of all ... daughters -in- law, forming a hierarchy attending to the business of factory or counting-house under the orders ofthe father ofthe family, and to the economy ofthe house-under the superintendence ofthe ... rescuing of all the oppressed Princesse Micomiconas, and the destruction of all windmills, and the capturing of all helmets of Mambrino, and the establishing all over the world ofthe worship of...