... activation of transmembrane substrates The activation of ADAM17 by furin [68] and < /b> our observation of an interaction between GRASP55 and < /b> the ICD of ADAM17 (C Roghi and < /b> L Jones, unpublished results) ... of GRASP55 and < /b> MT1-MMP in bbHT1080 Fixed and < /b> permeabilized bbHT1080 cells were incubated with antibodies directed against MT1-MMP (A, D, J), GRASP55 (G, K), TGN46 (E) and < /b> giantin (B, H) The co-localization ... lgÆmL)1, IB: 1.5 lgÆmL)1) was described previously [69] MT1-MMP rabbit polyclonal antibody and < /b> control rabbit IgGs were obtained from Insight Biotechnology Ltd (Middlesex, UK) MYC (4A6) (IB:1.5 lgÆmL)1)...
... untreated HepG2 Both bands further increased by 6.0- to 8.0-fold at h and < /b> decreased at h Another band, SNIII, had a rather abundant basal level, increased by 2.5- and < /b> 5.0-fold at and < /b> h, respectively, ... preincubated with Snail antibody, but not Raf antibody (the control antibody), for 30 (compare lanes and < /b> 8) At h, the blocking effect of the Snail antibody was less prominent because the amount of both ... activation of p15–pro228 by 10–20% (Fig and < /b> data not shown), probably because of the involvement of Lamin A in transcriptional regulation The effects of Snail shRNAs were verified by western blotting,...
... that binding of IIG6 disrupts the salt bridges between D669 and < /b> R168, and < /b> between D670 and < /b> R169 Cooperativity between IIG2 and < /b> IIG6 has been suggested to occur as a result of the breaking of site ... through binding site in the absence of site Occupancy of IIG2 has been proposed to disrupt site [36], by disruption of hydrophobic and < /b> salt bridges between G2 helix a1 and < /b> the C-terminal helix of ... presence of EGTA or calcium and < /b> apparent Kds of 0.8 and < /b> 3.5 lM, respectively, were determined (Fig 5B and < /b> Table 1) These values and < /b> those obtained above from ELISA show a better interaction of G2...
... 420883), plot of two independent batches of Ludox particles suspended in DI water and < /b> statistical analysis tables for cell viability, membrane permeability and < /b> lysosomal mass/pH parameters Abbreviations ... Institute (ERI) Building, Lee Road, Cork, Ireland 4Centre for BioNano Interactions, School of Chemistry and < /b> Chemical Biology, University College Dublin, Dublin4, Ireland Authors’ contributions BMM aided ... model of the in vivo first line of interaction between nanoparticles and < /b> human tissues, as it would be expected following exposure of the lungs (inhalation) and < /b> uptake of foreign material by phagocytic...
... WebLogo [32] by combination of the characterized binding sites and < /b> the results of the mutational analysis of the binding site at -445 van Verk et al BMC Plant Biology 2011, 11:89 http://www.biomedcentral.com/1471-2229/11/89 ... in one of the binding sites an A is present at +1, which in the W-box is usually either a C or a T To disable binding of WRKY28 to the 30-bp EMSA probe harboring the binding sites at -460 and < /b> -445, ... with expression of ICS1, is acting downstream of SA In the pbs3 mutant, accumulationof SA-glucoside and < /b> expression of PR-1 are drastically reduced The PBS3 gene product is a member of the auxin-responsive...
... the libraries were 216.4 bp for uninfected, 217.5 bp for library and < /b> 215.9 bp for the library (Figure 1) The pyrosequencing run was efficient based on the maximal amount of data obtainable being ... attraction of zoospores was investigated by Botha and < /b> Kotze in 1989 and < /b> it was found to be influenced by the composition of 14 amino Page of 14 acids in avocado root exudates [13] Sánchez-Pérez and < /b> ... Total Bases 288885 737254 1017064 Number of Contigs 43 139 189 The total number of reads and < /b> contigs are shown to illustrate the efficiency of the pyrosequencing run Mahomed and < /b> van den Berg BMC...
... gle and < /b> double mutants In situ measurements of trichome DNA contents of cpr5 sinIn situ measurements of trichome DNA contents of cpr5 single and < /b> double mutants (A) Interaction of cpr5 alleles and < /b> ... to be a Type IIIa membrane protein with five transmembrane domains and < /b> a cytoplasmic N-terminus This Page of 11 (page number not for citation purposes) BMC Plant Biology 2008, 8:58 http://www.biomedcentral.com/1471-2229/8/58 ... strong loss -of- function allele Trichomes of try-JC plants are larger and < /b> more branched than those of wild-type plants, and < /b> highly birefringent (Fig 1A, D) In contrast, the trichomes of double mutant...
... STUDY OF NEW AND < /b> ADVANCED CONTROL CHARTS FOR TWO CATEGORIES OF TIME RELATED PROCESSES DENG PEIPEI B. Sc., University of Science and < /b> Technology of China A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... parents and < /b> all my family members for their continuous care and < /b> precious support, and < /b> my boyfriend Yibo, for his understanding and < /b> illuminating encouragement in this endeavour ii TABLE OF CONTENTS ... the TBE control charts can be classified into two categories: variable TBE control charts and < /b> attribute TBE control charts The classification is based on the definition of the term “time” and...
... metabolism Amino acid metabolism Glycan biosynthesis and < /b> metabolism Energy metabolism Nucleotide metabolism Xenobiotics biodegradation and < /b> metabolism Metabolism of cofactors and < /b> vitamins Metabolism ... heredity of gynogenetic fry (G1) and < /b> non-gynogenetic fry (F6) of BSB with 1100 bp band amplified by primer S and < /b> 860 bp band amplified by primer S18 which was only found in F6 group and < /b> G1 group, ... magnesium (0.04%) and < /b> iron (0.024-0.048%) (Shi et al., 1997) By the feeding behavior of BSB, a non-animal ingredient was known suitable for digestibility The digestibility of BSB to non-animal...
... 2–3 and < /b> 4– 5) Notably, JSP1 seemed to stimulate preferentially the phosphorylation of the p46 isoform of JNK, whereas sorbitol treatment induced phosphorylation of both the p46 and < /b> p55 isoforms ... signal transduction pathways activated by stress and < /b> inflammation Physiol Rev 81, 807–869 Pearson G, Robinson F, Beers GibsonT, Xu BE, Karandikar M, Berman K & Cobb MH (2001) Mitogen-activated protein ... myristoylation in health and < /b> disease J Chem Biol, doi:10.1007/s12154-009-0032-8 Buss JE, Kamps MP, Gould K & Sefton BM (1986) The absence of myristic acid decreases membrane binding of p60src but does not...
... most of the proofs of the strong theorems are based on a joint application of the above two methods 4.3 Between the strong law of large numbers and < /b> the law of iterated logarithm The Theorem of Borel ... and < /b> results of this part of the probability theory, (e) some parts of this book can be used in any introductory or advanced probability course The main aim of this book is to collect and < /b> compare ... language of Rademacher functions The gambler will talk about coin tossing and < /b> his gain And < /b> a probabilist will consider independent, identically distributed random variables and < /b> the partial sums of...
... cleavage of Notch Both cleavages of APP- and < /b> Notch-based Ó FEBS 2003 substrates were modulated by known c-secretase inhibitors We found that inhibition of Ab secretion correlated with the observed ... Ab-secretion by Western blotting as described above Cells were washed and < /b> prepared for FACS analysis as described above The density Cell fluorescence c-secretase assay (Eur J Biochem 270) 497 of the Ab-bands ... cells (black solid line) after subtraction of the MLF of the mock-transfected cells (dotted line) (B) Comparison of the effect of the c-secretase inhibitors on Ab secretion (open bars) and < /b> cell...
... unavailability of a conformation-dependent e subunit mAb, only binding of antiFLAG mAb was tested CD spectra Binding of the rabbit anti -b serum to recombinant b1 –221 and < /b> human AChR When the b subunit ... ECDs or BSA as a control, and < /b> the binding of mAbs tested by ELISA as described in Experimental procedures (duplicate samples) mAb 73, checker-board bars; mAb 67, dark gray bars; FLAG mAb, light ... one a subunit and < /b> the c ⁄ e subunits and < /b> the other between the second a subunit and < /b> the d subunit [4] The c ⁄ e and < /b> d subunits play a major role in shaping the ligand-binding sites and < /b> also in...
... transcriptional activators, and < /b> this event would take longer time, and < /b> it could be blocked by MEK inhibition Second, activation by forskolin ⁄ IBMX at the protein level was not observed beyond h However, ... MOP-62745G), and < /b> Canadian Diabetes Association (CDA, 1198) to TJ, and < /b> CIHR and < /b> CDA operating grants to PLB PLB is a Canadian Research Chair, LC is a recipient of Banting and < /b> Best Diabetes Center Novartis ... speculate that forskolin ⁄ IBMX may stabilize Cdx-2 protein via a yet to be identified mechanism, and < /b> this effect cannot be blocked (or immediately blocked) by MEK inhibition Activated ERK1 ⁄ 2,...
... chemical units But it appears that we are barred from the present discussion of such a problem in absence of any theory of the solid state generally, but more particularly of those forms of matter ... influence preventing the recognition of the obvious and,< /b> as we think, inevitable basis of classification of the 'celluloses' is the empiricism of the methods of agricultural chemistry, which as ... original publication of our work on 'Cellulose,' there have appeared a large number of publications dealing with special points in the chemistry of cellulose So large has been the contribution of matter...
... i by Ci We then obtain a Bush-type Hadamard matrix of order 16n2 Example We give an example of a Bush-type Hadamard matrix of order 64 For ease of notation, we use − instead of −1 Let K be ... one whose first row and < /b> first column is all ones For matrices A, B, A ⊗ B is the tensor or Kronecker product of A and < /b> B the electronic journal of combinatorics (2001), #R1 2 Bush-type Hadamard ... system a +b+ c+d+e+f +g+h a +b+ c+d a +b+ e+f a+c+e+g a +b c−d−e−f +g+h a b+ c−d−e+f −g+h a b c+d+e−f −g+h = = = = = = = 4n 2n 2n 2n 0 It can be seen that the solution for this system is b = c = e = h and...
... presence or absence of 2-AP (10 mM) and < /b> analysed by gelatin substrate zymography The area (absorbance units) of substrate gel cleared by proMMP-9 and < /b> active MMP-9 was measured by scanning densitometry ... MMPs via induction of NF Band < /b> early response genes (c-fos and < /b> c-jun) Activation of PKR and < /b> subsequent phosphorylation of eIF2α would lead to an inhibition of protein synthesis and < /b> increased apoptosis, ... 24 hours and < /b> media were analysed for MMP-2 and < /b> MMP-9 activity by gelatin substrate zymography The presence of MMP-2 (Fig 1a) and < /b> of MMP-9 (Fig 1b) was confirmed by comparisons with standards known...
... pathways used by nerve growth factor and < /b> basic fibroblast growth factor (bFGF) in PC12 cells: rescue by bFGF involves protein kinase C delta Biochem J 2000, 352 Pt 1:175-182 Sopher BL, Thomas PSJ, ... of recombinant endostatins on neurite outgrowth (data not shown) and < /b> lack of binding of APmESXVIII and < /b> AP-mESXV to murine embryonal nerve tissues [19] AP-mESXVIII predominantly labelled blood vessels ... differentiation and < /b> acti- Publish with Bio Med Central and < /b> every scientist can read your work free of charge "BioMed Central will be the most significant development for disseminating the results of biomedical...
... activation of different types of C-H bonds by various metal fragments on per hydrogen basis 11 2.1 Changes in 3a:2a absorbance ratio with length of photolysis 35 2.2 Changes in 3b: 2b and < /b> 4b: 2b absorbance ... complexes can be reversibly displaced by CO In the case of the cobalt and < /b> rhodium complexes, the CO ligand is only weakly bound to the metal center Hence Ic and < /b> IIc converts back to Ib and < /b> IIb readily ... 14a and < /b> 1 4b 61 2.6 Summary of photochemical reactions of 2a – 2d, 7a and < /b> 7b 67 2.7 IR and < /b> NMR data of products 68 2.8 Crystal data for 10, 11 and < /b> 1 2b 77 2.9 Crystal data for 13, 14a and < /b> 1 4b 78...