... data were reweighted after selecting the model to obtain better estimates of kinetic parameters The dosage regimen of ceftriaxone was also determined based on the kinetic data The priming (D) ... 5% Each sample was diluted to the extent that its zone of inhibition came in linear range (preferably in the range of the zone of inhibition of the reference concentration) In this experiment, ... Table Calculated intravenous dosage regimen of ceftriaxone required to maintain specified plasma ceftriaxone concentration in febrile buffalo calves Desired plasma Dosage interval Priming dose...
... protein in the pellet fractions after h induction (Figure 2, panel E) When comparing gene expression in the different Ecoli strains, p24 was expressed at the highest level in strain HMS Rossetta ... RTB/p24 in the peritoneal cavity resulted in its transport to lymph nodes where the fusion could be internalized by antigen presenting cells and presented to T and B cells There are some instances in ... three cases, a band of 57 kDa was detected The fact that we were able to detect the same band by three different antibodies targeting different components of the chimeric RTB/p24 protein, demonstrate...
... cHSPA6 remained same when induced at early exponential to late exponential stage (Fig 6, lanes 2–4) but induction level was reduced when cells reached in the stationary growth phases (Fig 6, lane4) ... expression of cHSPA6 Lane 1, low molecular weight marker; 2, uninduced in NB; 3, induced in NB; 4, uninduced in LB; 5, induced in LB; 6, uninduced in 2× LB; 7, induced in 2× LB; 8, uninduced in ... performed at this temperature 2.2.2 Culture media optimization LB media showed relatively higher growth rate under induced culture conditions resulting in higher volumetric yield Figure Effect...
... involved in PLP-binding through a Schiff base linkage Comparison of the deduced amino acid sequence of oraE to those of known PLPdependent aminomutases reveals the presence of a conserved PLP-binding ... nucleotide sequence of the ora genes in order to facilitate the amplication by PCR An NcoI site was introduced into the start of the oraS gene and a BamHI site into the end of the oraE gene Genomic ... conditions The codon usage difference between C sticklandii and Ecoli does not seem to be responsible for this result, because the Ecoli strain, Epicarian Coli -Codon PlusTM (DE3)-RIL, contains extra...
... wild-type ovine STEC of different serotypes; group received K-12 prior to BLV challenge, and then STEC beginning at day post BLV challenge; groups and never received STEC Fecal STEC numbers were determined ... lymphocytes were analyzed independent of STEC treatment, among STEC treatment groups independent of STEC numbers, and between BLV-infected and BLV-free sheep carrying only naturally occurring STEC ... The correlation between STEC score and B-cell percentage in blood was positive in BLV-free sheep, and negative in BLV-challenged sheep harboring only naturally acquired STEC These results suggest...
... amount of encapsulated enzyme could not be detected The kinetic data obtained in presence of the FhuA Δ1-159 Ext, were compared to a set of negative controls to verify the obtained results In detail: ... Lys residues in total Seven of these are involved in closing the FhuA Δ1-159 Ext channel upon labelling: four are buried within the channel and three are present on both channel entrances (see Figure ... type) were used as standard reference Circular dichroism (CD) spectra were carried out for newly engineered FhuA Δ1-159 Ext to get an insite into the protein secondary structure The spectra were...
... pregnant females were inoculated with – × 108 live Ecoli organisms by direct injection into the Table 1: Effect of intrauterine Ecoli inoculation on preterm delivery Treatment Number of Ecoli ... After an initial learning period, we were able consistently to catheterize one uterine horn to the desired distance of cm and infuse it with the dye solution with retention of dye in the uterine ... published Figure intrauterine treatment on fetal viability Effect of1 Effect of intrauterine treatment on fetal viability Female rats on day 17 of pregnancy were inoculated with either sterile solution...
... genes, there are Ngene × (Ngene 1)/2 gene-gene pairs In order to find the score for gene A and TF B, we first seek all genes regulated by TF B in the preliminary TRN Then we calculate the gene-gene ... discover interesting gene-gene relationships; rather their potential to infer gene/TF interactions is very limited Therefore, the main assumption in constructing genegene networks, i .e that the TF ... illustrates the dependence of the percentage of recovery on the degree of incompleteness in the preliminary TRN As anticipated, more complete preliminary TRNs allow a higher percentage of the unknown...
... time at both challenges Overall time trend differences between challenges were tested with an F-test As there were different intervals between sampling, isotropic spatial exponential correlation ... between different cows The significant differences between the consecutive challenges seen here suggest that in these studies the interval between challenges should be longer than weeks Authors' ... pmol/min/μl after the second (range 0.63 – >2.5) After the first challenge NAGase activity remained elevated over the experimental period, but returned to the baseline value by this time after the...
... over time when repeated sampling is desirable, it appears more suitable to examine interstitial fluid that can be sampled frequently after it has entered the collecting vessels of the peripheral ... nodes of cows has, to the best of our knowledge, never been examined The purpose of the present study was to investigate the initial phase of the innate immune response in the mammary tissue and ... additionally influenced by the content of afferent lymph flowing into the lymph node [8,30,31] Thus, the degree of influence from different sources can be expected to vary dependent on whether the node...
... second-generation cephalosporins; 3&4GC, third-generation and fourth-generation cephalosporins P value for the linear regression coefficient and trend (increase, +; decrease, -) in parentheses penem-resistant ... because it has been shown that high level carbapenemresistant KPC-producing bacteria may be selected during imipenem and meropenem therapy [31] In contrast, the increase in 3GC-resistant Ecoli ... baumannii suggesting that carbapenem resistance developed after or during the spread of the clonal lineages [35] They explained that clonal lineages originated worldwide in at least eight distinct loci...
... Staphylococus aureus, Ecoli Klebsiella pneumoniae Năm 2011, Leena and Aleykutty nghiên cứu dịch chiết C Paniculatum Linn sử dụng 05 dung môi khác (ethyl acetate, alcohol, nước, petroleum ether, chloroform) ... ampicillin hãng Fermetas (E coli Top 10 pJET 1.2/blunt) vi khuẩn Ecoli Top 10 chứa plasmid pPS1 có gen kháng kanamycin Cornell University (E coli Top 10 pPS1), bảo quản tủ -800C, Bộ môn Công nghệ Sinh ... K Ampicillin (Am) 10 23 21 Amoxcillin (Ax) 10 17 19 Streptomycin (Sm) 10 19 21 Neomicin (Ne) 30 11 Gentamycin (Ge) 10 25 18 Kanamycin (Kn) 30 17 Amikacin (Ak) 30 16 18 Tetracyclin (Te) 30 10 10...
... Ampicillin, amoxcillin, streptomycin, gentamycin, norfloxacin, ofloxacin, colistin, sulfamethoxazol/trimethoprim; mẫn cảm trung bình với amikacin kháng lại kháng sinh penicillin, neomycin, kanamycin, ... chloramphenicol, streptomycin, sulphonamide tetracycline Theo nghiên cứu Zhao cộng năm 2003 Salmonella enterica serotype Newport isolates kháng lại loại kháng sinh (bao gồm cephalosporins) gây bệnh ... (Pellerin et al., 1998) Tương tự vậy, phát thấy tăng sức đề kháng với penicillin, neomycin, sulphonamide, co-trimoxazole erythromycin vi khuẩn S Intermedius phân lập từ chó Thụy Sỹ (Wissing et...
... chemicals by minimized reaction cascades ChemSusChem 5:2165- 2172 Hiroe A., Tsuge K., Nomura C.T., Itaya M., Tsuge T 2012 Rearr angement of gene order in the phaCAB operon leads to effective ... ultrahigh-molecular-weight poly[(R)-3-hydrobutyrate] in genetically engineered Escherichia coli Appl Environ Microbiol 78:3177- 3184 Jechlinger W., Szostak M.P., Witte A., Lubitz W 1999 Altered temperature ... Pitera D.J., Smolke C.D., Keasling J.D 2006 Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes Nat Biotechnol 24:1027-1032 22 Rollin J.A., Tam T.K.,...
... DANH SÁCH CÁC CHỮ VIẾT TẮT APC Antigen-presenting cell (Tế bào trình diện kháng nguyên) Ecoli Escherichia coli Ig Immunoglobulin (Globulin miễn) IL Interleukine KHT Kháng huyết KN Kháng nguyên ... citrate âm tính Không sử dụng phenylalanin, ure, gelatin, KCN (potasium cyanic), malonate, adonitol, inositol, không sinh H2S Do đó, Ecoli đƣợc phát khả lên men lactose sinh 44oC, có kết nghiệm pháp ... lại nhƣ lipopolysaccharide Escherichia coli, flagellin Salmonella, vỏ polysaccharide vi khuẩn Pneumococcus, dextrans, levans polyglutamic acid Bởi chúng trình tự polimer lặp lại nên phân tử gắn...
... sinh độc tố ruột EIEC (Enteroinvasive E. coli) : E. coli xâm nhập ruột EAEC (Enteroaggregative E. coli) : E. coli ngưng tập ruột DAEC (Diffusely adherent E. coli) : E. coli bám dính phân tán EHEC (Enterohaemorrhagic ... (ExPEC-extraintestinal pathogenic E. coli) - Các loại E. coli gây bệnh đường ruột (IPEC) biết gồm: EPEC (Enteropathogenic E. coli) : E. coli gây bệnh đường ruột ETEC (Enterotoxigenic E. coli) : E. coli ... CNPG Indole Đỏ methyl VogesProskauer Citrate (Simmons) Lysine decarboxylase Arginine dihydrolase Ornithine decarboxylase Di động D-Glucose acid E. coli (bình thường) + + + E. coli (inactive ) T...
... polymerase (tổng hợp) 5’ exonuclease 5’ 3’, exonuclease (thủy giải) 3’ 5’ 3’ Enzym DNA fragment Klenow enzym DNA polymerase I bị cắt bỏ tiểu đơn vị nhỏ (nhờ protease), phần chịu trách nhiệm exonuclease ... (catabolyte gen activator protein) hay CRP (cAMP recepter protein) phức hợp cAMPCAP cố định promoter làm tăng khả lyên kết RNA polymerase với promoter Nhằm trì tồn vector bacteriophage tế bào ... (đó operator) Trƣớc operon lacZ gen điều hoà I, gen mã hoá repressor, tức protein có vai trò kìm hãm biểu gen Hoạt động operon Lac đƣợc chứng minh nhƣ sau: Khi có glucose (và lactose), gen điều...
... them by IMViC test, then serotype with F4, F5, F6 antiserum Second, selecte Ecoli isolates of serotype F4 or F5 to produce the autovaccine Third, set up the experiment to evaluate the efficiency ... of auto-vaccine (the experiment was repeated in times): The first group: litters (28 piglets, from 14 to 20 days aged), vaccine injection one times The second group: litters (28 piglets, from 14 ... auto-vaccine was evaluated by antiody titers, medium daily gain and the side effects Result: The auto-vaccine made a high antibody titers and long immunization The auto-vaccine did not bring neither...