and cyclin dependent kinase inhibitors in cancer
Apoptosis, Senescence, and Cancer docx
... Gautier 12 Interactions Between Myc- and Cyclin- Dependent Kinase Inhibitors in Cancer 223 Kirsteen H Maclean, and John L Cleveland vii viii Contents 13 Interplay Between H2AX and 53BP1 ... (ATR) and DNA -dependent protein kinase Likewise, the relationship between c-myc and cyclin- dependent kinase inhibitors is considered in terms of tumor progression, as well as their relevance for cancer ... receptors Instead, TNF-R1 binds to the DD-containing adapter protein TNFR-associated DD (TRADD) (33) The DD of TRADD binds other DD-containing proteins, including FADD and receptor-interacting protein...
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
... maintained in RPMI-1640 containing 10% heat-inactivated newborn bovine serum (SiJiQing, Hangzhou China) at 37° C in a humidified atmosphere of 5% CO2 and 95% air Double immunofluorescence staining ... and an emission wavelength of 575 nm (A) Fluorescence intensity analysis of intracellular adriamycin in gastric cancer cells (B) Adriamycin releasing index of gastric cancer cells The releasing ... PI3K/Akt signaling pathway played a role in PrPC-induced MDR in gastric cancer cells, the PrPC-overexpressing cell line and the corresponding controls were treated with LY294002, a selective inhibitor...
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Báo cáo y học: "Two specific drugs, BMS-345541 and purvalanol A induce apoptosis of HTLV-1 infected cells through inhibition of the NF-kappaB and cell cycle pathways" ppsx
... cell cycle progression in G1 phase [46-50] Tax targets cell cycle regulators such as p53, cyclin dependent kinases (CDKs) and 6, cyclin D2, and CDK inhibitors p21waf1 and p16INK4A [51-57] Tax expression ... purvalanol A: a specific inhibitor of cyclin- dependent kinase activities Int J Cancer 2002, 97:761-769 Bain J, McLauchlan H, Elliott M, Cohen P: The specificities of protein kinase inhibitors: an update ... A, which is a purine analog that competes with the ATP binding site in CDKs, has been shown to inhibit cyclin E/CDK2 and cyclin A/CDK2 kinase activities with an IC50 of 0.035 and 0.07 μM, respectively...
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Báo cáo khoa học: Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN ppt
... contains two PAS domains (PAS A and PAS B) and a serine ⁄ threonine kinase domain that might be regulated in cis by binding of so far unknown ligands to the PAS domain [7] PASKIN shows a striking ... contains an oxygen-binding heme group within its PAS domain [5] Subsequent to de-repression by ligand binding, autophosphorylation in trans results in the ‘switch-on’ of the kinase domain of ... concentrations of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 even inhibited autophosphorylation, establishing a structure–function relationship between kinase domain–lipid interaction and kinase activity PtdIns-monophosphate-dependent...
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Báo cáo hóa học: " Synergistic Effect of Functionalized Nickel Nanoparticles and Quercetin on Inhibition of the SMMC-7721 Cells Proliferation" potx
... uptake of quercetin into cancer cells and enhance the cytotoxicity suppression of quercetin on the proliferation of cancer cells, indicating their great potential in clinical and biomedical applications ... relevant cell into cancer cells and increase the concentration of drug in cancer cells and thus could greatly inhibit the proliferation of cancer cells Hence, these observations indicate that ... crosslinking within unknown protein molecules in cells, the fluorescence of quercetin could be observed and detected [26] As shown in Fig 4, the apparent differences of drug accumulation in cancer...
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Báo cáo y học: "Glucosamine affects intracellular signalling through inhibition of mitogen-activated protein kinase phosphorylation in human chondrocytes" pps
... glucosamine was ineffective in preventing phosphorylation of this kinase JNK and p38 are involved in cellular stress; they are phosphorylated in response to proinflammatory cytokines, resulting in ... amino-terminal kinase (JNK) and total JNK, (b) p-p38 and total p38, and (c) p-extracellular signal-regulated kinase (ERK)1/2 and total ERK1/2 were used to visualize mitogen-activated protein kinase ... electrotransferred and immunoblotted Antibodies to (a) phosphorylated (p)-c-jun amino-terminal kinase (JNK) and total JNK, (b) p-p38 and total p38, and (c) p-extracellular signal-regulated kinase (ERK)1/2 and...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... (2001) Suppression of urokinase expression and Invasiveness by urinary trypsin inhibitor is mediated through inhibition of protein kinase C- and MEK/ERK/c-Jun – dependent signaling pathways J Biol ... role of protein kinases A and C pathways in the regulation of mitogen-activated protein kinase activation in response to gonadotropin-releasing hormone receptor activation Endocrinology 5, 2241–2251 ... to a scintillation vial containing 3.5 mL of INSTA-GEL scintillation fluid (Packard instrument Co., Meriden, CT, USA), and radioactivity was determined by liquid scintillation counting using a...
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Báo cáo sinh học: "Inhibition of mitotic kinase Aurora suppresses Akt-1 activation and induces apoptotic cell death in all-trans retinoid acid-resistant acute promyelocytic leukemia cells" ppt
... usage of VX-680 inhibition of Aurora kinase in ATRA-resistant APL remains unknown Here we showed that Aurora kinase small-molecule inhibitor VX-680 led to mitotic defects in spindle and decreased ... to be active in anticancer therapy with affinity for Aur-A (Ki = 0.6), B (Ki = 18), and C (Ki = 4.6) [34] VX-680 also inhibited other protein kinases, including Flt-3 (Ki = 30) and MAPK (Ki > ... Annexin V/PI staining and immunofluorescent staining with Hoechst 33342 Annexin V/PI staining showed that percentage of apoptosis were 3.66%, 5.52%, 15.83%, 24.43% respectively for 24 hr, and...
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báo cáo hóa học: " Pharmacological inhibition of Akt and downstream pathways modulates the expression of COX-2 and mPGES-1 in activated microglia" potx
... phosphatidylinositol 3 -kinase, mammalian target of rapamycin, glycogen synthase kinase- 3, Akt, prostaglandins FINDINGS Inflammation has been recognized not only as a mere bystander in neurodegenerative ... Lindsay TF, Fish JE, Marsden PA, Thuerauf DJ, Glembotski CC, Rubin BB: MAP kinase kinase 6-p38 MAP kinase signaling cascade regulates cyclooxygenase-2 expression in cardiac myocytes in vitro and ... significant information regarding the regulation of COX-2 and mPGES-1 via the Akt/mTOR and Akt/GSK-3 pathways, and suggest that interfering with these signalling cascades using pharmacological inhibitors...
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báo cáo hóa học: " Inhibition of gap junctional Intercellular communication in WB-F344 rat liver epithelial cells by triphenyltin chloride through MAPK and PI3-kinase pathways" pdf
... lethal effects of pharmacological cyclin- dependent kinase inhibitors in human leukemia cells proceed through a phosphatidylinositol 3kinase/ Akt -dependent process Cancer Research 2003, 63:1822-1833 ... Biosystems (Foster, CA, USA) The protein kinase C (PKC) inhibitor GF109203X, extracellular signal-regulated protein kinase (ERK) inhibitor PD98059 and PI3 kinase inhibitor LY294002 were from Sigma ... human tumor cells and tissues, including those involved in human mammary carcinoma, prostate cancer, human glioblastoma, skin squamous cell carcinoma, lung cancer, esophagus cancer, adrenocortical...
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báo cáo hóa học:" Inhibition of mitotic kinase Aurora suppresses Akt-1 activation and induces apoptotic cell death in all-trans retinoid acid-resistant acute promyelocytic leukemia cells" ppt
... usage of VX-680 inhibition of Aurora kinase in ATRA-resistant APL remains unknown Here we showed that Aurora kinase small-molecule inhibitor VX-680 led to mitotic defects in spindle and decreased ... to be active in anticancer therapy with affinity for Aur-A (Ki = 0.6), B (Ki = 18), and C (Ki = 4.6) [34] VX-680 also inhibited other protein kinases, including Flt-3 (Ki = 30) and MAPK (Ki > ... Annexin V/PI staining and immunofluorescent staining with Hoechst 33342 Annexin V/PI staining showed that percentage of apoptosis were 3.66%, 5.52%, 15.83%, 24.43% respectively for 24 hr, and...
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Báo cáo y học: "Resistance to IL-10 inhibition of interferon gamma production and expression of suppressor of cytokine signaling 1 in CD4+ T cells from patients with rheumatoid arthritis" ppsx
... regulating the function of both macrophages and Th1 cells [1] IL-10 inhibits the tyrosine phosphorylation of the CD28 molecule and the subsequent phosphatidylinositol 3 -kinase binding in T cells, and ... binding to JAK, and SOCS3 inhibits the action of JAK by binding to the Src homology phosphatase-2-binding domain of receptors such as gp130 [40] SOCS1 and SOCS3 are induced by various cytokines, ... ligands, CD80 and CD86, in the inflamed ST [18-20] The continuing emergence of activated CD4+ T cells, even though few in number, may be crucial in sustaining the activation of macrophages and...
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Báo cáo khoa học: "Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo" docx
... DNA-PKcs is approximately 470 kD consisting of 4128 amino acids Its C-terminus contains a serine/threonine protein kinase domain (3719- 4128) [23] For screening Page of 11 the specific anti-DNA-PKcs ... protein level in HeLa, HeLa-pcDNA and HeLa-DPK3-scFv cells (B) In vitro detection of Gy-induced DNA-PKcs activity using the Signa-TECT® DNA -Dependent Protein Kinase Assay System Immunoblotting ... DNA -dependent protein kinase catalytic subunit (DNA-PKcs) during gastric carcinogenesis and its clinical implications on gastric cancer Int J Oncol 2007, 31:859-866 Willmore E, Elliott S, Mainou-Fowler...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells (mean ± S.D.; ** indicates ... insulin and Glut2 gene expressions, proinsulin and insulin protein levels, accumulation of cytoplasmic granules including a, b, and δ granules, and insulin release in response to elevated glucose concentration ... LC: Distinct distribution of laminin and its integrin receptors in the pancreas J Histochem Cytochem 2002, 50(12):1625-1632 Hulinsky I, Harrington J, Cooney S, Silink M: Insulin secretion and DNA...
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Báo cáo y học: "Knockdown of Rab5a expression decreases cancer cell motility and invasion through integrin-mediated signaling pathway" ppsx
... structural proteins, including talin, vinculin, aactinin, and tensin etc; the other is regulating molecules, including paxillin, focal adhesion kinase (FAK) etc Moreover, integrins also involve in many ... depletion of Rab5a influented the internalization and recycling of integrins from the trailing edge to the leading edge, and interrupted the ‘outside -in signaling The assembly and activities of ... (Raf Ras binding domain) and GST-TRBD (Rhotekin Rho binding domain) were expressed by pGEX-RBD and pGEXTRBD (gift from Dr Xiang-Dong Ren) in E coli strain Rosetta (DE3) respectively and purified...
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Báo cáo y học: "Antimelanogenic effect of c-phycocyanin through modulation of tyrosinase expression by upregulation of ERK and downregulation of p38 MAPK signaling pathway" docx
... (black) and melanin content (grey) were measured (C) The expression of tyrosinase was determined by immunoblotting analysis (black) and RT-PCR (grey), using b-actin and GAPDH as internal standards, ... (black) and melanin content (grey) were measured (B) The expression of tyrosinase was determined by immunoblotting analysis (black) and RT-PCR (grey), using b-actin and GAPDH as internal standards, ... Phycocyanin protects INS-1E pancreatic beta cells against human islet amyloid polypeptide-induced apoptosis through attenuating oxidative stress and modulating JNK and p38 mitogen-activated protein kinase...
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báo cáo khoa học: " Statin-induced apoptosis via the suppression of ERK1/2 and Akt activation by inhibition of the geranylgeranyl-pyrophosphate biosynthesis in glioblastoma" potx
... regulated kinase 1/2 (ERK1/2) pathway and Ras/phosphoinositol-3 kinase/ Akt pathway [3,4] In malignant glioma cells, statins induce apoptosis by the activation of c-Jun N-terminal kinase 1/2 (JNK1/2) ... These findings indicate that μM and 2.5 μM of fluvastatin and simvastatin, respectively, are within the peak plasma values of fluvastatin or simvastatin that are likely to be achieved in vivo In ... statin-induced apoptosis to be significantly inhibited (Figure 3B-D) Statin-induced decrease in the expressions of phosphorylated ERK1/2 and Akt To identify the molecules involved in statin-induced...
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Báo cáo y học: "Cancer control through principles of systems science, complexity, and chaos theory: A mode"
... helpful in understanding of normal and abnormal cellular growth including cancer which is now seen as a system -in- failing These theories also point to a potentially new therapeutic paradigm of cancer ... mutations is increasing in this phase with a potential shortcut from entropy to a positive exponential growth leading to cancer, connecting 168 cancer and aging Downregulation of sirtuins genes ... control: changing disorganized complexity into an organized one through increasing differentiation, decreasing entropy by increasing complexity, and strengthening self-organization and self-adaptation...
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The transference of meaning through class of words denoting parts of the human body in english and vietnames
... teaching rules in teaching in general, teaching native language or foreign language in particular is combining theory and practice Providing exercises to practise and check theoretical knowledge in ... they are sitting 2.The part of garment covering the knee 42) Knuckle Any joint in the fingers esp those where the fingers joint the rest of the hand ( of animal) Knee or joint joining the leg ... skill in using hand ( e.g: She has a light hand with sewing), the active help ( e.g: Please, give me a hand with the washing-up [11; 537]) and the promise to marry in English Another part " brain",...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... structures in Fig S2) and cyclobrassinin [7] Interestingly, only cyclobrassinin (Fig 8A) exhibited an inhibitory effect on both BHs The type of inhibition caused by cyclobrassinin was determined from ... rutalexin, brassilexin, cyclobrassinin and camalexin (0.10 and 0.30 mm final concentrations) The phytoalexin that displayed an inhibitory effect was further examined in order to determine the ... some crucifer phytoalexins (e.g brassinin and camalexin) [14] and other chemicals (3-phenylindole) [13] could induce the biosynthesis of phytoalexin-detoxifying enzymes in plant pathogenic fungi...
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