and atrmr1 receptor homology regiontransmembrane domainringh2 motif protein
Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
... D1 and D2 receptor fusion proteins (D1–CFP and D2–YFP) incomparison with emission spectra of the cell popula-tions that co-express dopamine D1 receptor fusion protein (D1–CFP) and ... D1 receptor fusion proteins (D1–CFP and D1–YFP).cMeasured in cellco-expressing two dopamine D1 receptor fusion proteins (D1MUT–CFP – genetic variants of dopamine D1 receptor and ... D1 receptor. bMeasured in cell co-expressing dopamine D1 and D2fusion proteins (D1–CFP and D2–YFP).cMeasured in cellco-expressing dopamine D1 and D2fusion proteins (D1–CFP and D2R1–YFP...
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Báo cáo khoa học: Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck potx
... I)-X(6)8)-Y-X-X-(L or I) and arefound in the intracellular portions of the TCR complex c,d, e and f chains, as well as in other immunoreceptorsincluding the B-cell receptor [3] and several Fc receptors[4,5]. ... without washing.NMR of His-cTCRfThe protein was resuspended in 500 lLH2Oand50lLD2O, and the pH adjusted to 6 with dilute HCl. Standard1D and 2D NOESY and DQF-COSY experiments wererecorded ... mgg@soton.ac.ukAbbreviations: ITAM, immunoreceptor tyrosine-based activation motif; His-cTCRf, histidine-tagged cytosolic TCRf;PTK ,protein tyrosine kinase; SH2, src homology domain 2; TCR, T-cell receptor. (Received...
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Tài liệu Báo cáo khoa học: How disorder influences order and vice versa – mutual effects in fusion proteins containing an intrinsically disordered and a globular protein docx
... n2Þwhere [h]1 and [h]2correspond to the measured mean ellip-ticity values per residue of proteins 1 and 2, respectively, and n1 and n2correspond to the number of residues ofproteins 1 and 2.When ... con-tent of unordered stretches (50–59%) and a low con-tent in a-helices and b-strands (2–3% and 22–29%,respectively), whereas GFP is rich in b-strands (46%) and exhibits a low content in unordered ... indi-vidual proteins. M, molecular weight markers; TF, total protein frac-tion; SOL, soluble protein fraction; proteins purified by immobilizedmetal affinity chromatography (IMAC).Ordered and disordered...
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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt
... datasetof all protein RNA structures. This kind of methodwas proposed by Sipple [20], and has been applied to protein structure prediction, protein protein and pro-tein–ligand interactions ... number of groupsfor the prediction of protein structures, protein DNA and protein ligand interactions [18–35], such anapproach has never been applied to protein RNAinteractions.In the present ... energetic of protein RNA complexes.Knowledge-based potential functions have beenemployed in protein structure prediction [18–27], aswell as in the prediction of protein protein [25,28–30] and protein ligand...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
... LPS/IFN-inducedNO production Protein kinases such as PKA and PKC, and various factorssuch as tumor necrosis factor receptor 6 (TRAF6) and myeloid differentiation protein (MyD88) are reported toparticipate ... mitogen-activated protein kinase kinase;MyD88, myeloid differentiation protein; NF-jB, nuclear factor-kappaB; NO, nitric oxide; PKA, protein kinase A; PKC, protein kinase C;PP2A, protein phosphatase-2A; ... Effects of PKA inhibitors (KT-5720 and H8) and PKC inhibitors(Ro31-8220 and GF109203X) on the enhancement by TS of LPS/IFN-induced NO production in VSMC (A), and Western blot analysis ofPKCa...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... blue and 30 mMdithiothreitol) and then incubatedat 86 °C for 8 min. Typically, cell extracts (5–10 lgof protein) were separated by SDS/PAGE using the standardLaemmli system [30] with 13% and ... Western blot analysis of proteins in samplescollected in (A). 5 lgand1 lg of total protein in samples were separated by SDS/PAGE and immunoblotted with anti-UP12 Ig and anti-GroEL Ig,respectively. ... buffer,proteins were eluted from the resin with buffer C [0.1MTris/HCI (pH 8.0), 1% SDS, 2 mMEDTA and 20 mMdithiothreitol] and analyzed by SDS/PAGE and Westernblotting.Subcloning and deletion...
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Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot
... ribosomal protein S6 kinase and acetyl-CoAcarboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytesUlrike Krause*, Luc Bertrand and Louis HueHormone and Metabolic ... theprocess of activation of ACC and p70S6K by amino acids isinhibited by okadaic acid and calyculin A, two inhibitors oftype 1 and 2A protein phosphatase, and by incubation ofliver cells ... activate p70 riboso-mal protein S6 kinase (p70S6K) and acetyl-CoA car-boxylase (ACC) involved in protein and fatty acidssynthesis, respectively. In contrast, the AMP-activated protein kinase (AMPK),...
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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
... assay, and that the relationship between protein concentration and activity was linear (Fig. S1). The protein concentrationswere 1 lm for beryllium fluoride (BeF3) and BeF42)) inhibi-tion and ... in b-cells and neurons, SUR2Ain cardiac and skeletal muscle, and SUR2B in smoothmuscle and some neurons [1]. SUR2A and SUR2B areencoded by splice variants of a single gene, ABCC9, and differ ... state and thusincreased channel activation.Experimental procedures Protein expression and purificationA FLAG tag was inserted into rat SUR2 between Ala1026 and Asp1027. Full-length SUR2A and...
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Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc
... transthyretin+TLPsequences, three motifs were identified,A’–C’. Motif A’ is equivalent to motif A fromthe TLP motif set. Motif B’ is similar butextended in the N-terminal and C-terminalregions to motif B. Motif C’ ... dublin TLP and zebrafish TLP are found in the flex-ible portions of strands B and C that protrude towardsthe solvent and in the conformation of the long loopconnecting strands D and E [17]. ... C-terminalregions to motif B. Motif C’ is shorter than motif C and its location is shifted towardsthe N-terminus. Motif D is specific to theTLP set of proteins. (C) Motifs A’–C’ weresuperimposed on the tertiary...
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Báo cáo khoa học: Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata pot
... (1980) Exita-tion profiles, absorption and resonance Raman spectraof the carotenoprotein ovorubin, and a resonanceRaman study of some other astaxanthin proteins. JAmChem Soc 102, 6693–6698.19 ... 40,175–215.30 Randall D, Burggren W & French K (1997) Energyacquisition: feeding, digestion and metabolism. In Eck-ert Animal Physiology. Mechanisms and Adaptations(Randall D, Burggren ... quantities of protein uti-lizing the principle of protein- dye binding. Anal Bio-chem 72, 248–274.34 Davis B (1964) Disc electrophoresis. II. Method and application to human serum proteins. Ann...
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Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artificially introduced cross-links in proteins and protein complexes potx
... cross-linking and mass spectro-metry for mapping three-dimensional structures ofproteins and protein complexes. J Mass Spectrom 38,1225–1237.7 Wong SS (1991) Chemistry of Protein Conjugation and Cross-Linking. ... thestructural analysis of proteins and protein complexes and in large scale analysis of the function of genes(proteomics) [1].For mass spectrometric analysis, the proteins and proteomes under study ... Koster1 and Luitzen de Jong11 Biomolecular Mass Spectrometry group, Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands2 Laboratory of Protein Biochemistry and Protein...
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Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx
... estrogen and androgen,because use of antagonists to androgen and estrogenreceptors (flutamide and ICI 182,780, respectively), and letrozol, an inhibitor of the aromatase responsiblefor converting androgen ... and then toestradiol by the P450 aromatase. It has been shownthat approximately 35% of cervical carcinomas expressaromatase [37] and that DHEA binds to the androgen receptor and estrogen receptors ... R(2005) Dehydroepiandrosterone inhibits the prolifera-tion of HUVEC by enhancing the expression of p53 and p21, restricting the phosphorylation of RB, and isandrogen- and estrogen -receptor independent....
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Báo cáo khoa học: Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia coli transthyretin-related protein potx
... Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia colitransthyretin-related protein Erik Lundberg1, Anders Olofsson2, Gunilla T. Westermark3 and ... tetrameric and monomericstructures.Thermostability and protein structuresWe have analyzed the structures of hTTR (Protein Data Bank code: 1F41 [12]), sbTTR (Protein DataBank code: 1SN2, [16]) and ... C, and D, of thetetrameric structures were: hTTR, A10–A122, B10–B122, A¢11–A¢122, and B¢10–B¢122; sbTTR, A10–A122, B10–B122, C11–C122, and D10–D122; and ecTRP, A4–A114, B4–B114, C5–C114, and D4–D114.cThe...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
... a-helices and 12%b-turns (Fig. 1). Incubation of the protein with dif-ferent ligands such as palmitate, caprylic and arachi-donic acids and retinol in concentrations sufficient tosaturate the protein ... ethanol and embedded in paraffin. ForR. Jordanova et al. Conformation, ligand binding and distribution of nematode protein Ag-NPA-1FEBS Journal 272 (2005) 180–189 ª 2004 FEBS 187Conformational and ... nonpolar and com-pletely isolated from the solvent.Here we analyze the conformational and functionalproperties of Ag-NPA-1 as well as the tissue and cellulardistribution of the protein. The...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... extracts a single band was recog-nized at 43 kDa indicating the presence of the full-length protein, in mitochondria three bands at about42, 43 and 44 kDa were detected, while no bands werevisible ... couldassume that in mitochondria the unprocessed protein, the mature protein and a post-translationally modified protein might be present. N-terminal sequencing and analysis of peptides by MS could help ... d-CDes protein was fusedwith the C terminus of the green fluorescent protein (GFP) in the pGFP-C vector the fusion protein remained in the cytoplasm (Fig. 4C).Expression studies on the RNA and protein...
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- listening and speaking
- mode sensors and switches
- a structure found in numerous proteases and implicated in protein binding
- and cterminal ring finger and pest domains homologous proteins include grail gene related to anergy in leukocytes
- a plant protein storage vacuole sorting receptor
- was recently identified in human peripheral blood monocytes treated with monocyte chemotactic protein 1 mcp1 and in human monocytederived macrophages stimulated with interleukin il1b these experiments revealed that the gene undergoes rapid and potent
- in this series of four minireviews the field of scaffold proteins and proteins of similar molecular⁄cellular functions is overviewed by binding and bringing into proximity two or more signaling proteins
- metallothionein mt was first identified in 1957 by m margosch and b vallee as a cadmium protein from equine kidney cortex in fact
- c reactive protein and cancer
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