... Effects of n-3 and n-6 PUFAs on the cyclin D1 gene promoter We studied the mechanism(s) underlying the inhibition of cyclin D1 geneexpressionby EPA and DHA by examining the effects of n-3 and ... DHA could exert antiproliferative effects by interfering with Ras/Raf pathways Inhibitory effects of n-3 PUFAs on cyclin D1 geneexpression and inhibition of IL-1b-induced Egr-1 gene activation ... autoradiogram of three independent semiquantitative RT- PCR experiments, whereas the histogram shown in the lower part of the figure represents data from real-time quantitative RT- PCRanalysis Semiquantitative...
... decomposition of the RNA Determination of the level of BACE mRNA and ribozyme RNA expressionin mammalian cells The level of BACE mRNA and ribozyme RNA was monitored by RT/ PCR using a OneStep RT/ PCR kit ... level of BACE protein, and in consequence, to reduce the amount of Ab peptide released in neuronal cells We asked the question whether we can inhibit expressionof the geneof BACE protein using ... used in RT/ PCR At postincubation time h (12 h after the beginning of transfection, see Materials and methods) only a minimal amount of ribozyme RNA was present in the cell lysates The level of...
... < 0.01) (Fig 1A,B) The inhibition of insulin signalling in the liver is primarily reflected by the attenuation of the insulin-mediated inhibition of gluconeogenic geneexpression As FEBS Journal ... with insulin incubation coordinately regulate liver-specific geneexpression [42] Their transcriptional regulation, particularly that of PEPCK by insulin, is protein synthesis independent, but involves ... alterations in PEPCK geneexpressionIn this study, we found Foxa2 to be predominantly localized in the nuclei of HepG2 cells incubated with TNFa prior to insulin incubation As reported earlier, insulin...
... creating plasmid, pRS197; (d) pRS197 was transformed into RAS46 and recombinant strains carrying the chromosomally inserted plasmid (by means of single homologous recombination) were identified by ... containing the anaerobic respiratory chain (Figs and 2) Three other lines of evidence derived from in vivo and in vitro studies of nif regulation further supported our model: first, analysisof ... Gavini, N (1999) Genetic analysisof nif regulatory genes by utilizing the yeast two-hybrid system detected formation of a NifL-NifA complex that is implicated in regulated expressionof nif genes...
... mechanisms involved in the increase or maintenance ofbone mass in OA This study of the IT region of the proximal femur in primary hip OA and non-OA postmortem controls investigated changes ingeneexpression ... findings [24] This indicated an increased extent of both bone formation and bone resorption with age, which together suggest an increase in the rate ofbone turnover with ageing in control individuals ... in OA and a decrease in controls [16] This finding is supportive of an increase or maintenance ofbone volume in OA individuals versus the agedependent bone loss in the general population [11]...
... hours postinfection At hours post-infection, the level of previously elevated cytokines/chemokines still remained at several folds of induction In contrast to the prominent induction of cytokines/chemokines ... differences in the profiles of cytokines/chemokines induced by influenza viruses of different pathogenicity H5N1 was a more potent inducer of inflammatory cytokines/chemokines; particularly TNF-a, CXCL-10/IP-10, ... prominent during the early phase of infection with H5N1/2004 and H9N2/1997 viruses than those induced by H1N1/2002 virus At the late phase of H5N1 infection, more intense induction of cytokine/chemokine...
... protein-coding genes, with 6,117 genes remaining after filtering dubious open reading frames (classified by the Saccharomyces Genome Database; Additional data file 8) In the case of RA data, as in ... overall branching pattern of the tree generated was characterized by two large groups: in one of them (clusters 1-8) most clusters showed profiles in which rates (points and in the profile) were ... proteins were one of the most uniform groups (Figures and 4) Nevertheless, as shown also in Figure 3, six genes encoding proteins of this group showed a variant profile characterized by an inversion...
... byin situ hybridization Cytogenet Cell Genet 39, 220-223 Lefevre F & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon ... orientation of close genes (Morton et al., 1984), The - - - bridging the gap between the physical and the genetic maps, of genes close enough to be considered as genetically linked - by the assignment ... 349-360 Lin C.C., Draper P.N & De Braekeleer M (1985) High-resolution chromosomal localization of the 0 -gene of the human ,Q-globin gene complex byin situ hybridization Cytogenet Cell Genet 39,...
... interleukin gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin gene; IFN-ind: the interferon-inducible gene 6-16; IL4R: the interleukin receptor gene; TAP2: the transport ... of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP A1 ... lymphomas In this group of upregulated genes there were the a-myb oncogene, the interleukin receptor gene (IL4R), the geneof the transporter protein TAP2, the geneof protease calpain small subunit...
... cannot determine the global players involved in such complex phenotypes Integration of the geneexpression profiling with specific modulation ofgeneexpressionin relevant signalling pathway ... expression Genes detected and in following investigated for validation A) an induced expressionof the PKN1 gene B) a repressed expressionof the ZCD1 gene C) a repressed expressionof BSG gene It ... regulated genes were detected after processing and analyzing with Illumina’s BeadStudio Data Analysis Software and are described in the following part according the definitions of the Illumina’s...
... next determined geneexpressionin different adipose depots in response to PM2.5 exposure, in terms of the expressionof BAT-specific and WAT-specific gene profiles by real-time PCRanalysis UCP1 ... determined by counting the number of positive stained nuclei in 10 random fields Quantitative real-time PCR Total RNA was isolated using TRIzol reagent as instructed by the manufacturer (Invitrogen, ... also increased in mBAT in response to PM2.5 exposure We did not observe significant differences in the gene expressions of Hoxc9 or Igfbp3 in the WAT, neither was the geneexpressionof Dpt in...
... PDK mRNA expression, selective inhibitors of signalling pathways known to be activated by insulin were used Two distinct phosphatidylinositol 3-kinase (PtdIns3K) inhibitors, wortmannin and 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyan-4-one ... selectively inhibited by the mitogen-activated protein kinase kinase (MEK) inhibitor, U0126 [32,33] Therefore, the role of the MAPK pathway in regulating PDK mRNA expression was investigated by incubating ... regulated independently by the main fuel sources in muscle and by insulin through directly altering the expressionof the human PDK2 and PDK4 isoforms In addition to our findings, insulin has been shown...
... protein response genes in Arabidopsis vesicle trafficking ⁄ transport, vacuolar protein sorting, cell wall biogenesis, and lipid ⁄ inositol metabolism In comparison with those of yeast, the UPR genes ... genes of the 27 000 proteincoding genes of Arabidopsis with an Affimetrix GeneChips [32] In this paper, we present a list of the UPR genes of Arabidopsis identified among all the protein-coding ... confirmed by real-time RT- PCRanalysis (Fig 3) In addition, we found that an antiapoptosis protein, Bax inhibitor-1 (BI-1) [35,36], was also included in the list of up-regulated UPR genes Induction of...
... intron of the ANX4 genein OVTOKO and OVISE cells but not in MCAS cells, indicating that endogenous p53 protein directly binds to the ANX4 genein CCA cells To verify the involvement of p53 in ... p53 binding To examine whether endogenous p53 actually binds to these regions in CCA cells, we performed chromatin immunoprecipitation (ChIP) assays using PCRanalysisof the p53 binding domains ... Comprehensive geneexpression profiling has revealed that the pattern ofgeneexpressionin CCA cells is clearly distinct from that of other EOC cells [11,12] In particular, the annexin IV (annexin A4,...
... differences inexpressionof genes encoding enzymes and other proteins involved in intermediary metabolism Perivenous expression is indicated by green, and genes with preferential periportal expression ... FEBS A Braeuning et al Zonal geneexpressionin mouse liver separation of hepatocytes into periportal and perivenous subfractions was determined by real-time RT- PCRanalysisof GS expression and ... Zonal geneexpressionin mouse liver A Braeuning et al enzymes of serine ⁄ glycine metabolism: Glycine decarboxylase, a component of the glycine dehydrogenase complex that converts glycine to serine,...
... the expressionof ecdysone receptor B1 isoform in imaginal wing cells of P interpunctella at the last larval instar In the fifth larval instar of M sexta, it has been reported in vitro that 20E induced ... isolation, DNA minipreps were prepared and correct insertion was determined by restriction enzyme analysis The DNA clone containing the proper insert was sequenced by the dideoxy chain termination method ... results indicate that PIEcR is a B1 type isoform Effect of 20E and anisomycin on induction of PIEcR-B1 mRNA Using a PIEcR-B1 specific probe located in the N-terminal region of A/B domain, the Northern...
... cytokinins and hormones The main purpose of a regulated geneexpression system is to control the timing and levels of transgene expressionin vivo Whether the expressed protein remains within the ... Ras-related GTP-binding proteins that are localized in distinct intracellular compartments [24] Mutations in the Rab gene can alter the morphology of entire organelles by blocking protein transport along ... expressionof host genes AMK, RG-121150, affects geneexpressionin 293 cells The affect of the AMK RG-121150 on geneexpressionin 293 cells was analyzed using microarray and qRTPCR In microarray analysis, ...
... predictive values of a panel of miRs byquantitative realtime PCRin formalin-fixed paraffin-embedded tumor specimens from 639 resected NSCLC patients participating in the International Adjuvant ... now being exploited in cancer, attempting to modulate their expression, reintroducing microRNAs lost in cancer, or inhibiting oncogenic microRNAs by using anti-micro oligonucleotides [38] In a ... Hospital of North Norway, Tromso, Norway 2Institute of Clinical Medicine, University of Tromso, Tromso, Norway 3Department of Pathology, University Hospital of North Norway, Tromso, Norway 4Institute...
... area of the condyle The areas of India ink staining were outlined using a permanent marker Tracings of the India ink-stained tibial and femoral condyles were evaluated without knowledge of dog ... assessments The lamina splendens was not disrupted in any location based on lack of India ink staining, indicat- ing that AC surface integrity was maintained for two weeks in the dogs in this study ... Hydroxyproline content of cartilage by region Hydroxyproline content of cartilage by region The HP content of each cartilage region from the ACL-X joint was compared to the corresponding region in the...
... classification of some of the genes of interest Confirmation ofgeneexpression changes by Real-Time PCRanalysis Ten genes were selected for real-time polymerase chain reaction (PCR) They are RNA binding ... CINP NUFIP1 IQGAP2 nucleosome assembly protein 1-like destrin proline rich utrophin histidine-rich calcium-binding protein anillin GTP-binding protein, member RAS oncogene family alpha tubulin ... and analysis Real-time of four selected genes using array A comparative analysisPCR A comparative analysisof four selected genes using array analysis and Real-time PCR RNA binding motif protein...