... PhK5 might bind with Trp357 in the N-terminal site in CaM, or perhaps could bind in the opposite orientation to incorporate the Trp into the larger C-terminal domain binding site In summary, ... Kay LE, Krinks M & Bax A (1991) Tripleresonance multidimensional NMR study of calmodulin complexed with the binding domain of skeletal muscle myosin light-chain kinase: indication of a conformational ... large aromatic residue in the N-terminal part of the peptide that binds in the with the C-terminal domain in CaM However in PhK5 Trp357 is found at the C-terminal end of the peptide This suggests...
... checkpoint signaling in vivo It may function in the sensing of DNA damage [22] In contrast with the intensive study of RPA in animals and yeast, little is known about this protein in plants In 1997, ... experiment, the indicated proteins were incubated together in NaCl ⁄ Pi containing 250 mM NaCl at °C for h, and then incubated with Talon beads The amount of protein present in the fraction bound ... of OsRPA70a recombinant protein was found in the soluble fraction of OsRPA70a–OsRPA32–OsRPA14 extracts but most of it failed to bind to the GST column (Fig 4A), indicating that it could not interact...
... referring to all of them as caveolae, was demonstrated by their content of both caveolin-1 and caveolin-2 The coexistence of caveolin-1 and caveolin-2 in all three caveolae subclasses is in line ... indicated protein or EZ-Link-sulfo-NHS-LC-biotin labelling by SDS ⁄ PAGE and immunoblotting or avidin-labelled HRP, respectively The amounts of indicated proteins are expressed in arbitrary densitometric ... normalized to percentage of maximum (A) Protein concentration (mgÆmL)1) (B) Caveolin-1 (C) Caveolin-2 (D) Cell surface biotin labelling of proteins (E) TGN38 (F) Insulin receptor (G) GLUT4 (H)...
... [12–18,20]: *, CTP-binding residue of the P-loop; (*), CTP-binding residue; ^, Neu5Ac-binding residue; #, Neu5Ac-binding residue forming part of the hydrophobic pocket; °, residue required in the quaternary ... significant increase in Km(Neu5Ac) would support a role in binding that substrate In order to investigate this further we made a series of mutations at Gln104 We maintained the length of the side chain ... pocket plays a role in substrate discrimination against binding of the 5-OH group of Kdo and in favour of the N-acetyl group of Neu5Ac and, although these residues are conserved in CNS enzymes, none...
... presence of any traces of AMP, either endogenous or as a Regulation of AMPK a2b2c3 complexes by AMP A B C result of the presence of AMP- generating proteins as contaminants [47] Several upstream kinases ... explain the mechanisms by which the mutations in CBS domains of AMPK c3 interfere with activation by AMP However, our data would be consistent with a single AMP- binding site being present in c3 ... whereas the two pairs of CBS domains both bind one molecule ofAMP [20] In terms of the structure of AMPK, the b-subunit has been reported to act as a scaffold for binding of the a-subunit and...
... tail and the binding of H1.4 to chromatin, as arginine offers additional hydrogen-bonding abilities to DNA as compared to lysine Lysine 173 in H1.4 is situated in an SPKK motif, one of the known ... the binding of H1.2 to chromatin, may be affected A further nonsynonymous substitution prompted the replacement of lysine with arginine in the C-terminal tail (position 173) of histone H1.4 in ... concentration of solvent B was increased linearly from 15 to 23% during a time-period of 25 min, from 23 to 70% during a time-period of 45 and from 70 to 100% during a time-period of obtained from...
... by institutional structures, policy initiatives, or clinical concerns? Developments in cancer research in the 1990s, including rapid advances in understanding of cancer genetics and increasing ... in each set of specialty journals Here, instead of selecting and mapping some portion of the most specific links, we mapped only the links between the nearest nodes, as follows The strength of ... translational interface exists Circulation, however, the sole clinical mix journal in the set, maintains links to both the clinical domain and the molecular biology domain This journal plays a key role linking...
... exploration training, one third of their patients demonstrated an increase in VF size of – 7° Reading training had a similar effect: In 34% of hemianopic patients who participated in reading training, ... performance in manipulating things, visually orienting, walking around, driving or reading In only two studies so far the effects ofvisual treatment on daily living have been investigated Within the ... ongoing investigations with the help of functional imaging including fMRI The importance of attention in recovery In patients with severe damage to visual cortical areas V1 and V2 resulting in...
... China has a single, endemic genotype However, continued sampling of measles virus strains from the different locations around China is needed for a more complete understanding of their evolving ... [8,27-29] Since WPRO, including China, has recently initiated a program to eliminate measles in 2012, maybe a variety of genotypes will be detected in China as the intensity of the measles control ... the predominant cluster circulating in China in 2004 There were multiple lineages in each cluster These data reinforce the observation that multiple chains of transmission were present in areas...
... by institutional structures, policy initiatives, or clinical concerns? Developments in cancer research in the 1990s, including rapid advances in understanding of cancer genetics and increasing ... in each set of specialty journals Here, instead of selecting and mapping some portion of the most specific links, we mapped only the links between the nearest nodes, as follows The strength of ... translational interface exists Circulation, however, the sole clinical mix journal in the set, maintains links to both the clinical domain and the molecular biology domain This journal plays a key role linking...
... activities According to the International Statistical Classification of Diseases, Injuries and Causes of Death, visual impairment includes both low vision and blindness Low vision is defined as visual ... our findings It is interesting that a recent national survey of the UK system for delivering care to low vision subjects, involving a wide range of service providers, also found regional inequity, ... the institution survey, weights included size of strata, the institution occupation rate (number of subjects in the institution/number of available beds), and the answer refusal rate (higher in...
... frequency and waveform of a single-tone sinusoid using an offline-optimized adaptive filter,” in Proceedings of IEEE International Conference on Acoustics, Speech, and Signal Processing (ICASSP ’05), ... term in (18), which has constant coefficients of the powers of z Now, He (z, α) can be written in the form of He,o (z) in (17) as N −1 He (z, α) = − hn (α)z−(n+1) and solving for Δθs , the updating ... solution of Δθs However, in such case, e p will become nonlinear in Δθs This is avoided by equating the correction Δθs to zero in such a singular case, where μ is set to zero whenever (20) (21) sin...
... at Electronic Engineering degree of the University of Pisa He is currently engaged in research on biomedical engineering at the Information Department, Faculty of Engineering of Pisa University ... programming at Biomedical Engineering Degree of Pisa University She is currently a Biomedical Engineering Researcher at the CNR Institute of Clinical Physiology of Pisa Her research activity is mainly ... Faculty of Pisa He is actually an Associate Professor in biomedical engineering at the Engineering Faculty of Pisa Since 1992, he teaches regular courses on biomedical signal and image processing...
... arginines binding to the minor groove [50] This suggests that the weak DNA binding of the carboxyl terminus of CENP-C may be mediated by the arginines of the CENPC motif, with the remainder of ... proteins would maintain an interface with the conserved kinetochore machinery Indeed, regions of CENP-C that show evidence of positive selection include DNAbinding and specificity regions, in parallel ... evolution of CenH3s occurs in regions that have been implicated in DNA binding [9,11] No positive selection was observed for the poorly mapped carboxy-terminal DNA-binding domain in our slidingwindow...
... More interestingly, opsin gene expression changes during ontogeny in mbuna but not in the sand-dwelling cichlids of Lake Malawi A high proportion of the total larval opsin gene expression in tilapia ... divergence in sexual display coloration in the males of some cichlid species, in addition to a shift in the perception of these colors But what happens to opsin gene evolution in cichlid fish in crystal ... the evolution of the visual system A wider range of species data will help determine how common heterochronic shifts in opsin expression are In addition, spatial localization of opsin genes to...
... 90 M.-B Turrión et al INTRODUCTION In natural ecosystems, amounts and chemical nature of soil organic P are mainly determined by a combination of the major soil-forming factors (time, parent ... given in tables I and II The climate of the area is characterized by rainy autumn and spring, and hot, dry summers; some winters are also dry and dryness is intense during summers, principally in ... 85% H3PO4 in a mm tube inserted into the 10 mm sample tube before the measurement of each sample Peak assignments were according to Newman and Tate [19] and Condron et al [5] Intensities of signals...
... similarities in the protein structure, originate from changes in the polygenic complex of regulatory genes, examination of their variability is of importance a-Amylase in Drosophila, active in the ... group of F/F lines which is at least + SE below the mean (U 7.00, P < 0.05), as well as between the groups of lines of both genotypes within ± SE of the mean (U 24.00, P < 0.05) The analysis of intergroup ... gene-enzyme systemsin Drosophila (see [9]) The analysis of the enzyme activity represents a model for distinguishing between the effects of the structural and regulatory genes involved in the control of...
... PiT1-binding immunoadhesin generated by fusing the aminoterminal residues of the GALV (SEATO strain) Env, comprising the signal peptide, the RBD and the proline-rich region, to the rFc tag, herein, ... any PiT2 staining The arrow points at a characteristic stained nigral cell as shown in the inset (magnification x300) D: PiT2 immunostaining in the cerebellum of a normal mouse Purkinje cells ... PiT1 staining in the cortex of control mice; stained neurons are mostly detected in layer II/III These neurons are medium-sized with homogeneous cytoplasmic staining B: PiT1 immunostaining in the...
... Actinidia species differ significantly in their timing of budbreak, winter-chilling requirements, lengths and numbers of nodes per shoot, indicating a genetic control of shoot growth and flowering ... Page of 15 Figure Expression profiles of Actinidia flowering genes in normal and aberrant flowers Real-time RT-PCR analysis of the Actinidia flowering genes in the leaf and floral organs of A ... biology of MADS box genes in a range of angiosperms has been instrumental in the development and testing of these models [reviewed in 58] and further broad comparative studies, including normal...