... that the proceduredescribed in this work for the activation of ONC has nodetrimental effect on the conformational stability of the enzyme.Cytotoxic activity The effect of (Met1) removal on the ... simplify the protocol and shorten the time needed to obtain a fully active ONC compared withother procedures described in the literature. The application of MALDI-TOF MS to the characterization of ... analyzed by MALDI-TOF MS. The addition ofthe protease is considered the zero time point. The relative intensities ofthe peaks from the mass spectrawere used to calculate the fraction of Pyr-ONC (M23L),...
... G 654 , G 752 , A 753 , and C 754 in the junction are blue. The OH group ofthe highly conserved Tyr68contactsG 752 O3Ј, while the side-chain ring packs tightly against C 754 . S 15 residues in the ␣1-␣2 ... fold, while the S 15 contactsabove the internal loop of helix 22 and to the tetraloop of helix 23a stabilize the tertiary fold of the upper three-helix junction. The tertiarystructure ofthe upper ... regions ofthe central domainRNA are not involved in directing assembly of the domain but rather are displayed on the sur-face of a preassembled RNP core. Hence, for-mation ofthe core of the...
... loop in the primed side ofthe binding sitegroove. Although the prodomains of cathepsins F andW share the same degree of similarity to the prodo-main of cruzipain, the similarity ofthe mature ... cross-inhibition of brucipain by PCZ. The pro-domain of cruzipain shares the highest identity with the C-terminal portion (residues Pro147–Leu 251 ) of the prodomain of cathepsin F (32%) and that of cath-epsin ... promisingtargets for the development of new drugs. The maintargets of these compounds arethe well-characterizedmajor CPs of T. cruzi, cruzipain (or cruzain) [5 7], and the CP of T. brucei, brucipain...
... stabilization ofthe carbon cationby the catalytic nucleophile. The new reducing end is thentransferred to the hydroxyl group at the 3 -position of the nonreducing end of another a cceptor ... Gas1p could play the role of catalytic residues.These predictions have been supported experimentallybecause the replacement of these residues with glutamineabolished the activity of Gas1p, whereas ... study on the effects of reducing conditions on the transport of Gas1p from the ER to the G olgi, w hich had indirectly revealed the requirement of at least one disulphide bond for the exit of Gas1p...
... of )3 15 ± 5 mV (ox/sq) and )3 65 ± 15 mV (sq/hq)derived from fitting titration data at the semiquinoneabsorption maximum (58 5 nm) to the 2-electron Nernstfunction (Fig. 6). The tendency ofthe ... potentiometry. The midpointreduction potentials ofthe oxidized/semiquinone ()3 15 ± 5 mV) and semiquinone/dihydroquinone ()3 65 ± 15 mV)couples ofthe FAD/NADPH domain are similar to thosefor the FAD/NADPH ... The presence ofthe His-tag on the FAD/NADPH domain had no apparent effecton catalytic activity. The purified domains were yellow, indicating the presence of bound cofactor, and they displayed...
... determinant ofthe hydrophobic SÂ2 bindingpocket of trypsins is the side-chain of Tyr 151 [18]. The importance of this residue is underlined by the fact that in the case ofthe second Kunitz-domain of ... raising the possibility that the inability of WFIKKN-KU2 to inhibitthese proteases is partly due to the lack of such a favorableinteraction ofthe PÂ2 Trp with the target enzymes. The factthat the ... with the fact that all homo-logues of WFIKKN-KU2 are known to contain b-strandsand a-helices in equivalent positions [14–19]. In view of the fact that the structure ofthe Kunitz inhibitor of the...
... 8,960 54 26 80 421 459 880 Tennessee 6,422 4030 70 50 6 59 1 1,097 Texas 397 11 9 20 34 24 58 Vermont 718 58 32 90 32 19 51 Virginia 54 ,333 37 27 64 5, 141 6,374 11 ,51 5 Wisconsin 6 35 32 35 67 55 37 ... with the exception ofthe action of this State and that of Georgia the important measureswhich actually proscribed the teaching of Negroes were enacted during the first four decades ofthe nineteenthcentury. ... moresuccessful. These educators adopted the threefold policy of instructing Negroes in theprinciplesof the Christian religion, giving them the fundamentals ofthe common branches, and teaching them the...
... experience, and then became quite independently the source ofthe goring-ox laws ofthe Book of Exodus. 75 He left the exact source undefined: the biblical rules derive their inspiration from these earlier ... Introduction: The Basic Thesis and Background 15 ofthe epilogue otherwise presumably facilitated CC’s use ofthe essence of this later transitional introduction in the formulation of 21:1.In the broader ... from 19 35: 22 The days when opinion concluded from the substantial agreement of legal principles in two different codes to the derivation ofthe later from the earlier code, without further qualification...
... 0 .52 0.44 0.46Speaking 0.30 0.49 0. 15 0 .56 Cleaning mouth 0.16 0 .53 0.36 0.49Sleeping 0.19 0 .53 -0.04 0 .57 Emotion 0.42 0 .50 0. 15 0 .55 Smiling 0.38 0.47 0.41 0.46Studying 0.13 0 .54 0.24 0 .55 Social ... burden, the use of pictures have been used in the Thai version of Child-OIDP[1,21] while a shorter version ofthe OIDP based only on the assessment of frequency of oral impacts has been usedin other ... 50 - 75 yearsold. Med Care 1991, 29:243- 250 .34. Fournier L, Kovess V: A comparison of mail and telephoneinterview strategies for mentalhealth surveys. Can J Psychiatry1993, 38 :52 5 -53 3. 35. ...