Genetic study of hematopoiesis in zebrafish characterization of zebrafish udu mutant, positional cloning and functional study of udu gene

... GENETIC STUDY OF HEMATOPOIESIS IN ZEBRAFISH — CHARACTERIZATION OF ZEBRAFISH UDU MUTANT, POSITIONAL CLONING AND FUNCTIONAL STUDY OF UDU GENE LIU YANMEI (Master of Medicine, Peking University, ... differentiation of hematopoietic cells in udu- /- 94 4.1.2 Identification of the udu mutant gene 100 4.1.2.1 Positional cloning of udu gene 100 4.1.2.2 Confirmation of identity of the udu gene ... Positional cloning and gene structure of udu gene 108 Figure 4.12 The nonsense mutation in Ensemble Gene 108 tu24 ENSDARG00000005867 (udu gene) in udu and udusq1zl mutants Figure 4.13 udu cDNA...

Báo cáo khoa học: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis doc

... organism for cloning the genes encoding the different fatty acid desaturases involved in EPA biosynthesis and identiﬁed two sequences coding for fatty acid desaturases with D5- and D6-regioselectivities ... contains 12 acidic amino acids (aspartate and glutamate), whereas the corresponding domain sequences in PtD5p and PtD6p have only eight and ﬁve acidic residues, respectively Such a decrease in ... the number of acidic amino acids in fused cytochrome b5 domains has been observed in Fig Amino acid sequences of PtD5p and PtD6p For alignment the CLUSTAL X program was used (gap opening 12, gap...

Báo cáo y học: " Genetic variations of nucleoprotein gene of influenza A viruses isolated from swine in Thailand" ppsx

... highly pathogenic avian influenza were retrieved from the database and included in the analysis human, classical swine and Eurasian swine lineages (Fig 1) The avian NP lineage contains all avian influenza ... (PB1), avian lineage (PB2, PA), Eurasian swine lineage (NA, M) and classical swine lineage (HA, NP, NS) [14,15] This serves as an example that certain influenza A strains can harbor an NP gene that ... divided into distinct lineages, Eurasian swine lineage and classical swine lineage Based on topology of the phylogenetic tree, the Eurasian swine lineage is closely related to the avian lineage and...

Báo cáo khoa học: Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination pdf

... appearance of other d-amino acids This is the ﬁrst report, for eukaryotes, of cDNA cloning and functional characterization of d-AAT acid aminotransferase in A thaliana tion rate of main roots and hypocotyls ... between d-amino acids, was cloned and characterized This represents the ﬁrst cDNA cloning and functional characterization of a d-AAT of eukaryotic 1196 origin Further characterization of this ... completely abolished activity, suggesting that A thaliana d-AAT is a Discussion D-Amino acids in A thaliana Free d-amino acids and conjugated forms of d-amino acids have been detected in higher plants...

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

... characterization of the human VGSC b1B subunit The human b1B subunit is a novel splicing variant of the b1 subunit via alternative intron retention The retained intron encodes a novel extracellular, ... peptide Functional expression of the human b1B subunit with NaV1.2 in Xenopus oocytes To explore the regulatory function of the human b1B subunit, we injected cRNA of human b1B, as well as cRNA of the ... 2003 Cloning and characterization of Na+ channel b1B (Eur J Biochem 270) 4763 reaction (RT-PCR) and RACE-PCR Marathon-ReadyTM human adrenal gland and fetal brain cDNA libraries were purchased...

Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx

... Results Cloning of the 5¢-upstream region of the Pokemon gene To identify the regulatory sequences that control expression of the Pokemon gene, a 2204-bp section of the 5¢-upstream region of the Pokemon ... mechanisms of the Pokemon gene Computer analysis of putative transcription factor-binding sites For a rough understanding of the regulation of the Pokemon gene, the 2204-bp section of its 5¢-upstream region ... 2008 The Authors Journal compilation ª 2008 FEBS 1861 Analysis of upstream region of the Pokemon gene Y Yang et al Fig Nucleotide sequence of the 5¢-upstream region of the Pokemon gene The upstream...

Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

... E.P., Martin-Eauclaire, M.F., Mansuelle, P & Sampieri, F (1991) An anti-insect toxin puriﬁed from the scorpion Androctonus australis Hector also acts on the alpha- and beta-sites of the mammalian ... Sampieri, F & Granier, C (1991) An anti-insect toxin puriﬁed from the scorpion Androctonus australis Hector also acts on the a- and b-sites of the mammalian sodium channel: sequence and circular dichroism ... obtained was displayed an ORF of 258 bp encoding a polypeptide of 85 amino acids and termed BmKIM The 5¢ and 3¢ UTRs of BmKIM cDNA are 17 bp and 76 bp, respectively A single AATAAA polyadenylation...

Genetic study of hematopoiesis development by two zebrafish mutants ugly duckling and tc 244

... GENETIC STUDY OF HEMATOPOIESIS DEVELOPMENT BY TWO ZEBRAFISH MUTANTS: UGLY DUCKLING AND TC- 244 DU LINSEN (B.Sc, Zhejiang University, China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... analysis of zebrafish mutants udu and tc- 244 in this study, two novel genes (udu and tc- 244) are identified as novel factors involved in the regulation of primitive and definitive hematopoiesis development ... genetic study of two zebrafish hematopoietic mutants: ugly duckling (udu) and tc- 244 Phenotypic analysis of udusq1 mutant allele showed that udu gene was essential for primitive hematopoiesis development...

Báo cáo y học: " Protocol for investigating genetic determinants of posttraumatic stress disorder in women from the Nurses'''' Health Study II" pot

... analyses Integration of this project with the larger NHSII study This study will take advantage of the resources of the ongoing NHSII study, whose core functions including the infrastructure of ... after the interview If the error is detected again in following interviewers, the interviewer is removed from the study Use of highly structured CATI interviews with well-trained carefully monitored ... set of analyses that test for heterogeneity in terms of the timing, type, and severity of the trauma We hypothesize that the effect of PTSD genetic risk variants will be magnified among women whose...

Báo cáo y học: " Full genome comparison and characterization of avian H10 viruses with different pathogenicity in Mink (Mustela vison) reveals genetic and functional differences in the non-structural gene" pot

... Zohari et al., Full genome comparison and characterization of avian H10 viruses with different pathogenicity in Mink (Mustela vison) reveals genetic and functional differences in the non-structural ... of the influenza virus [19] The amino acid composition of the receptor binding pocket of the HA protein for the H10 isolates is typical of avian influenza viruses The H10 viruses have histidine ... factor other than the presence of multiple basic amino acids in the cleavage site contributed to the severity of H10 viruses in mink Neuraminidase Haemagglutinin Phylogenetic analysis of the HA...

Báo cáo y học: "4G/5G polymorphism of PAI-1 gene is associated with multiple organ dysfunction and septic shock in pneumonia induced severe sepsis: prospective, observational, genetic study" doc

... The glycoprotein serine protease plasminogen activator inhibitor-1 (PAI-1) is a key element in the inhibition of fibrinolysis The primary role of PAI-1 in vivo is fast acting inhibition of tissue- ... shows genetic association between the 4G allele of 4G/5G polymorphism and the occurrence of MODS and septic shock in pneumonia sepsis Comparing the allele frequencies of PAI-1 4G/5G polymorphism of ... in evaluating the effect of PAI-1 4G/ 5G polymorphism on MODS, sepsis severity and mortality in a relatively homogeneous cohort of patients of the same ethnicity Moreover, this study is the first...

Báo cáo y học: "Influence of genetic variability at the surfactant proteins A and D in community-acquired pneumonia: a prospective, observational, genetic study" pot

... clinical evaluation of patients, samples and data collection PS participated in the statistical analysis NGQ, IS and EHR did genotyping CRG conceived the study, analyzed and interpreted data, and wrote ... overrepresented among patients who died at 28 or 90 days, and they also predisposed to MODS and ARDS respectively Likewise, 6A was associated with ARDS, and 1A was associated with MODS By contrast, 6A3 and ... with CAP Haplotypes 6A1 2, 1A1 0 and 6A to 1A are overrepresented among patients who died at 28 or 90 days By contrast, 6A3 and 6A3 to 1A1 are protective against 28-day and 90-day mortality Additional...

Functional characterization of HGF and its receptor c met in zebrafish development

... paracrine signaling in liver development The coexpression of hgfa and c- met in pectoral fin, hgfb and c- met in proneprhic duct also indicate their paracrine signaling in the development of these ... Characterizing HGF and its receptor c- met s role in zebrafish development (Manuscript in preparation) v LIST OF FIGURES Fig.1.1 Schematic representation of proHGF/SF, HGF/ SF and the c- Met receptor ... X CHAPTER INTRODUCTION .1 1.1 Discovery of HGF and its receptor c- met 1.1.1 Discovery of HGF 1.1.2 Discovery of c- met and identification of c- met as the receptor...

Characterization of pin1 function in zebrafish development

... 1.1.3.1 Pin1 function in M phase 1.1.3.2 Pin1 function in G1 and S phase 1.1.3.3 Pin1 function in oncogenesis .9 1.1.4 Pin1 function in apoptosis 10 1.1.5 Pin1 function in ... reduction of zPin1 expression in zPin1 morphant embryos 79 Figure 3.8 Developmental delay in zPin1 morphant embryos 82 Figure 3.9 Neurogenin1 staining of the wild type embryos and catch up zPin1 morphant ... occur in Pin1deficient PGCs, resulting in fewer PGCs in the absence of Pin1 (This figure was adapted from Atchison et al., 2003) 18 Introduction 1.1.7 Pin1 function on protein stability Pin1 has...

Molecular characterization and developmental analysis of interferon regulatory factor 6 (IRF6) gene in zebrafish

... mouse, Fugu, and zebrafish 63 -64 3.5 Assembled sequences of irf6 genomic fragments 64 -66 3 .6 The irf6 gene locus on the LG22 in T51 RH panel and in the Ensembl zebrafish version (ZV6) 68 -69 3.7 Whole-mount ... morpholinos 85 3.14 Loss of irf6 function phenotypes 86- 87 3.15 Comparison of expression of molecular markers in irf6 morphants and wildtype 91-92 3. 16 Hematoxylin and eosin staining of intestines ... MOLECULAR CHARACTERIZATION AND DEVELOPMENTAL ANALYSIS OF INTERFERON REGULATORY FACTOR (IRF6) GENE IN ZEBRAFISH BEN JIN (M.Sc., National University of Singapore) A THESIS SUBMITTED...

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