out of the green yonder molecular cloning and functional characterization of beta carotene 15 15 apos oxygenases

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

Ngày tải lên : 16/03/2014, 23:20

... signiﬁcantly in the presence or absence of the b1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence of the b1 subunit The effects of b1B were further assessed ... in Fig 4A–D, the rates of activation and inactivation of the sodium current via 4768 N Qin et al (Eur J Biochem 270) Ó FEBS 2003 Fig The eﬀect of b1B and b1 subunits on the function and expression ... part of intron adjacent to exon (Fig 1C and Table 1) As the site of divergence between the b1 and b1B subunit cDNAs was located precisely at the exon 3/intron boundary of the SCN1B gene, the human...

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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

Ngày tải lên : 31/03/2014, 09:20

... carried out in accordance with the guidelines laid down by the National Institutes of Health in the USA regarding the care and use of experimental animals and committee giving approval for the experiments ... cDNA The yield from the initial screening of the cDNA library with the 32P-labeled cDNA probe was about 260 positive clones On the ﬁnal screening, 25 clones were selected on the basis of the strength ... Construction of expression vectors and expression of the fusion proteins The cDNA encoding BmKIM was ampliﬁed by PCR with forward and reverse primers The noncoding regions, the signal peptide and the...

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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

Ngày tải lên : 18/02/2014, 18:20

... al Zn-binding motif and the two disulﬁde bonds respectively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure ... 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... the two-metal-ion mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the ﬁndings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the...

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Báo cáo khoa học: Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination pdf

Ngày tải lên : 30/03/2014, 04:20

... appearance of other d-amino acids This is the ﬁrst report, for eukaryotes, of cDNA cloning and functional characterization of d-AAT acid aminotransferase in A thaliana tion rate of main roots and hypocotyls ... d-amino acids, was cloned and characterized This represents the ﬁrst cDNA cloning and functional characterization of a d-AAT of eukaryotic 1196 origin Further characterization of this d-AAT is necessary ... in the insoluble fraction Therefore, the coding regions of these ATs were subcloned into another expression plasmid, pET-41a(+), instead of pRSET-B DNA sequences of the coding regions of these...

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Báo cáo khoa học: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis doc

Ngày tải lên : 31/03/2014, 09:20

... Because of the relative insensitivity of front-end desaturases and elongases to the presence of an x3-double bond and of x3-desaturases to the structure of the carboxyl end of the fatty acids, the ... that four different routes led to the synthesis of EPA in P tricornutum and that the preferred route relied on intermediates of both the x6- and the x3-pathway (see Fig 1) The results presented ... the product of the x3-desaturase, 18:3D9,12 ,15, to 18:4D6,9,12 ,15 (see Fig 1) The selectivity of PtD6p was assayed in the presence of 125 lM of both 18:2D9,12 and 18:3D9,12 ,15 so that the four potential...

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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

Ngày tải lên : 23/03/2014, 10:20

... dsRNA They suggest that the complete silencing of the gene transcript could not be achieved due to dilution of dsRNA in the feeding stage of the female tick [31] On the other hand, Acosta and coworkers ... observed the amplification by RT-PCR of a defined band of 260 bp in the saline treated group only These results suggest the possible complete degradation of the CHH transcript because of dsRNA ... cysteine residues at the same positions as that of other CHHs of penaeid shrimps (Fig 1B) Tissue-specific gene expression of L schmitti CHH gene The size and expression of the CHH mRNA in different...

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Báo cáo sinh học: " Molecular cloning and partial sequence characterization of the duplicated amylase genes from Drosophila erecta" pps

Ngày tải lên : 14/08/2014, 20:20

... both probes were identified Their DNA was isolated and digested with Sal A southern blot (Southern, 1975) of the digested DNAs was made, and probed with the pOR-M7 and CS.4a probes, as described ... apart from the others, and the divergence time has been estimated to be as high to 15 million years Since some of our data concern the coding region of the gene, they can be used to confirm the above ... hypothesis of recombination events occuring between the copies of the gene within a locus We cloned both copies of the amylase gene from D erecta and sequenced part of the coding region (containing the...

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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

Ngày tải lên : 18/02/2014, 11:20

... glycinin and b-conglycinin are synthesized and translocated into the ER lumen during the maturation stage of embryogenesis Therefore, we next measured the mRNA and protein levels of GmPDIL-3a and ... top of the gradient is on the left, and density (gÆmL)1) is indicated on the top (B) Microsomes were treated without (lanes and 2) or with (lanes and 4) proteinase K, in the absence (lanes and ... the presence of MgCl2 In the presence of EDTA, which releases ribosomes from the rough ER [24], the peaks of GmPDIL-3a and GmPDIL-3b were shifted to the lighter sucrose fractions and therefore had...

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Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulﬁde isomerases as molecular chaperones for seed storage proteins doc

Ngày tải lên : 07/03/2014, 05:20

... b-conglycinin during the course of the folding process of these proteins Results Cloning and expression of GmPDIL-1 and GmPDIL-2 To clone the soybean ortholog of Arabidopsis PDI-like 1-1 and PDI-like ... present in the putative boundary region between b and b¢ in GmPDIL-1, and between b and b¢ and b¢ and a¢ in GmPDIL-2, the structure of these regions may be protease resistant The activity of recombinant ... formation in the ER and may cause the accumulation of unfolded proglycinin As the active sites of the PDI family proteins are reduced in the ER in the presence of dithiothreitol, neither GmPDIL-1...

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Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc

Ngày tải lên : 07/03/2014, 10:20

... of its alkaloids One of the alkaloids produced by this plant is the antimicrobial sanguinarine, which had been used as the component of toothpastes and mouthwashes The biosynthetic pathways of ... and open circles are untreated controls The relative expression level shows the values standardized by that of control (0 h sample) as The error bar indicates the standard error of the mean of ... identiﬁcation and characterization of other members of the CYP719 family, especially in evolutionally older plant species, should provide clues to better understand the diversiﬁcation of the CYP719...

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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

Ngày tải lên : 07/03/2014, 15:20

... between the fully active alpha isoform and other variants, we conducted a series of cotransfection experiments and compared ligand-induced accumulation of cAMP (Fig 3) Although the level of cAMP ... CRFR1 a, b, c and d isoforms differ in their ability to bind ligands and activate G proteins [10,16,25] CRFR1a is the most efﬁcient in the stimulation of cAMP production, CRFR1c and CRFR1b have ... assembled by PCR of these two fragments with primers E3 and E11 Two different constructs were produced for CRFR1h isoform The ﬁrst contained exons 1–4 and a fragment of the cryptic exon up to the translation...

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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Ngày tải lên : 07/03/2014, 21:20

... VBARP Also, the use of unsynchronized cells in these analyses further confirmed that the cytoplasmic distribution of VBARP is independent of the cycling stage of the cells Identification of biological ... further confirming the specificity of this interaction Based on these results we were able to demonstrate the formation of a complex between Vpr and VBARP Characterization of VBARP variants The ... VBARP-S expressed the predicted molecular mass of protein similar to the in vitro 4095 Molecular characterization of ANKHD1 splice variant A B Fig Expression of VBARP isoforms in vitro and in vivo:...

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Báo cáo khoa học: Sulfation of hydroxychlorobiphenyls Molecular cloning, expression, and functional characterization of zebraﬁsh SULT1 sulfotransferases docx

Ngày tải lên : 08/03/2014, 02:20

... elucidate the dose-dependence of the regulation of the activity of the zebrafish ST by these divalent metal cations and their modes of action Fig Effects of divalent metal cations on the sulfating ... identification of the various cytosolic STs and their biochemical characterization We report in this communication the molecular cloning and expression of two distinct zebrafish cytosolic STs The enzymatic ... dopamine and T3, whereas zebrafish SULT1 ST2 was more active toward the thyroid hormones (T3 and T4), estrone, and dopa Whether these activities reflect truly the physiological functions of the two...

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Báo cáo Y học: Molecular cloning and characterization of isomultiﬂorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt

Ngày tải lên : 08/03/2014, 23:20

... with Kpn I and Xba I, and ligated to the sites of pYES2 (Invitrogen), to obtain pYES2-LcIMS1 in which the ORF of the cDNA was ligated to the GAL1 promoter in the sense orientation The nucleotide ... cDNAs of OSCs have been cloned from higher plants, there are few reports [6,14] on their gene expression in the context of the physiological regulation of sterol and triterpenoid biosynthesis Molecular ... except the stationary phase, the bryonolic acid level increased during the late growth phase only [16,17] The level of isomultiﬂorenol synthase mRNA was high at days 16 and 20 and low at days and The...

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Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme potx

Ngày tải lên : 16/03/2014, 18:20

... the diﬀerences the substrate speciﬁcity loop is coloured green in the case of Pf LDH and red in the case of Pf MDH Comparative expression of Pf MDH, Pf LDH and Pf MQO and effect of gossypol The ... only 26% between the two enzymes On the other hand the structure appears to be similar to that of Pf LDH with a rmsd of 0.8 between the Ca atoms (Fig 5) Both the cofactor and the substrate binding ... buffers (Fig 4) The optimum pH for oxidation of malate in the presence of NAD was 10.2 and for the reduction of OAA using NADH as the cofactor it was 7.0 Further kinetic characterization of enzyme...

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Báo cáo khoa học: A novel plant protein disulﬁde isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx

Ngày tải lên : 30/03/2014, 04:20

... comprised of molecular chaperones and other functional proteins must be strengthened in response to the increased de novo synthesis of seedstorage proteins Therefore, we next measured the mRNA and ... Lys150 (K150) and R255 by N-terminal sequencing of the trypsin peptide fragments The N-terminal sequences of other fragments generated by protease digestion were AHHHHH and corresponded to the ... (AtPDIL2-2 and AtPDIL2-3) were identiﬁed (supplementary Fig S1) Therefore, it is possible that one of the bands is GmPDIM and the other is a homolog However, it cannot be excluded that the second band...

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Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx

Ngày tải lên : 30/03/2014, 04:20

... EMSAs To further determine the cooperation between the NEG-U and NEG-D elements, lg of the NEG-U decoy, lg of the NEG-D decoy, and a combination of lg of each of the decoys were used, and the above-mentioned ... Analysis of upstream region of the Pokemon gene Role of the NEG-D element in the negative regulation of the Pokemon promoter F-560 The NEG-U element alone cannot negatively regulate the function of the ... regulation of the Pokemon promoter B Fig The NEG-U and NEG-D elements are necessary for the negative regulation of the Pokemon promoter (A) The left-hand panel shows the F-560 and mutant constructs The...

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Molecular cloning and characterization of sq163, a zebrafish liver mutant

Ngày tải lên : 14/09/2015, 14:06

... columnar and polarized It forms the epithelial lining of the whole of the digestive tube except part of the mouth, pharynx and the terminal part of the rectum Expectedly, it also contributes to the ... linings of all the glands which open into the digestive tube, including those of the liver and pancreas, the epithelium of the auditory tube and tympanic cavity, the trachea, bronchi, and alveoli of ... of hemoglobin in Kupffer cells) enter the intestine, and then leave the body in the feces By-products in the blood (such as urea) are filtered out by the kidneys, and then leave the body in the...

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Molecular cloning and expression analysis of a heat shock protein (Hsp90) gene from black tiger shrimp (Penaeus monodon)

Ngày tải lên : 11/11/2016, 15:59

... reagent following the protocol of the manufacturer, and resuspended in DEPC-treated water and stored at –80°C Synthesis of the cDNA first strand Materials and methods Shrimp About 40 appear healthy ... together and formed a group, respectively In the tree, the black tiger shrimp shows the closest relationship with the C haematocheir, the result is similar with the result of the BLAST So the ... binding [36] The motif also overlaps with the GA binding motif [37] The presence of the EEVD motif at the C-terminal end suggests the cytosolic localization of btsHsp90 [38, 39] And the functional...

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Tài liệu Báo cáo khoa học: Tachykinin-related peptide precursors in two cockroach species Molecular cloning and peptide expression in brain neurons and intestine docx

Ngày tải lên : 20/02/2014, 01:20

... demonstrated the occurrence of the different TKRPs in glomeruli of the tritocerebrum and in cells of the optical lobe Results Cloning of cockroach preprotachykinin cDNAs Using degenerated PCRs and RACE, ... copies of different TKRPs of all invertebrates studied so far Most of the 14 15 peptides predicted on each of the precursors could be identiﬁed in the brain and intestine of the two cockroach species ... number of TKRP immmunopositive cells in the brain of L maderae [16] Mainly due to good accessibility, immunoreactive glomeruli of the tritocerebrum and neurons in the vicinity (inner side) of the...

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