molecular and functional characterization of p glycoprotein in vitro

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Ngày tải lên : 07/03/2014, 21:20

... A B Fig Expression of VBARP isoforms in vitro and in vivo: (A) In vitro transcription ⁄ translation of VBARP-L and VBARP-S One microgram of VBARP-L, VBARP-S and vector plasmid was in vitro transcribed ... role in promoting proliferation and preventing apoptosis [8] dMASK mediates receptor tyrosine kinase (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream of MAPK or ... transcribed to cDNA and PCR ampliﬁed using One Step RT-PCR Superscript II (Invitrogen) and primers speciﬁc for VBARP and b-actin (housekeeping gene) molecules The following VBARP primers were designed:...

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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

Ngày tải lên : 21/02/2014, 00:20

... which provides open and closed conformation to the protein during binding of the substrate is shown in the top-most portion of the protein ATP may bind to the glycine loop on the left flap portion ... abundance of AK mRNA during the growth curve of promastigotes Functional characterization of recombinant AK The recombinant protein purified from the inclusion bodies and refolded in the presence of ... presence of several concentrations of ATP (A) or AMP (B) and different concentrations of the inhibitor Each point is the mean of three separate trials Inhibition of AK by the specific inhibitor Ap5A and...

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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

Ngày tải lên : 07/03/2014, 15:20

... Corticotropin-releasing factor type and type alpha receptors regulate phosphorylation of calcium/cyclic adenosine 3¢,5¢-monophosphate response element-binding protein and activation of p4 2 /p4 4 mitogen-activated ... Perkins, A.V., Europe-Finner, G., Lowenstein, P. R & Linton, E.A (1996) Corticotrophin-releasing hormone receptor type 1: generation and characterization of polyclonal antipeptide antibodies and ... with HindIII and EcoRI and inserted in the vector described above This plasmid was named pLuc The minimal promoter containing TATA box was ampliﬁed from pcDNA6/V5-HisA vector (Invitrogen) by primers...

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Cellular and molecular control of skeleton formation in fish insights from osteoblast ablation and functional characterization of lrp5 and SOst

Ngày tải lên : 10/09/2015, 08:43

... the intracellular domain of Lrp5/6 contains Axin2 binding sites (Mao et al., 2001) Thus, binding of Axin might be the trigger for inactivation of the phosphorylation of β-catenin The binding ... SmaI and SalI and ligated into the digested target vector 2.3.9.3 TOPO/pDrive cloning of riboprobe templates Purified PCR products of partial lrp5 and crestin transcripts were ligated into the pDrive ... Lrp5 and Sost are conserved at the sequence level Fig 11 Early embryonic expression of lrp5 and sost Fig 12 lrp5 and sost expression at 24 and 48 hpf Fig 13 72 hpf and dpf expression of lrp5 and...

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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

Ngày tải lên : 18/02/2014, 18:20

... helicase ⁄ NTPase domain are indicated in gray and black respectively (B) Puriﬁcation of the recombinant Rep245 protein SDS ⁄ PAGE of protein extracts at various stages of the puriﬁcation of Rep245 Lane ... have counterparts in Pfuprimase, although not in the pRN1 prim–pol protein In archaeal and eukaryotic primases, the K135 residue (the counterpart of R148 in Pfu-primase) is part of a highly conserved ... Supporting information The following supporting information is available: Fig S1 The in uence of (A) divalent cations, (B) pH and (C) temperature on Rep245 polymerase activity This supporting information...

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Báo cáo khoa học: Sulfation of hydroxychlorobiphenyls Molecular cloning, expression, and functional characterization of zebraﬁsh SULT1 sulfotransferases docx

Ngày tải lên : 08/03/2014, 02:20

... 3,3¢,5-Triiodo-L-thyronine Thyroxine Estrone Dopamine L-Dopa D-Dopa Dehydroepiandrosterone p- Nitrophenol 2-Naphthylamine 2-Naphthol Daidzein Kaempferol Caﬀeic acid Genistein Myricetin Quercetin Gallic acid ... contain the Ô3¢-phosphate binding (3¢-PB) motifÕ (residues 135–143 for SULT1 ST1 and residues 137–145 for SULT1 ST2; underlined) responsible for the binding to the 3¢-phosphate group of PAPS [23] ... corresponding to SULT1 ST1 and cDNAs was conﬁrmed by nested PCR using the primary PCR products as templates in conjunction with their respective 5¢-primers and primers corresponding to sequences in...

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Báo cáo khoa học: Molecular and genetic characterization of osmosensing and signal transduction in the nematode Caenorhabditis elegans docx

Ngày tải lên : 30/03/2014, 03:20

... member of the transient receptor potential (TRP) family of cation channels TRP channels are divided into TRPC, TRPV, TRPM, TRPML, TRPP, TRPN and TRPA subfamilies All TRP channels are comprised of ... accumulation of incomplete and aberrantly folded polypeptides in the cytoplasm The majority of the protein homeostasis genes identiﬁed in our RNAi screen function in RNA processing, protein synthesis and ... encode proteins required for RNA processing, protein synthesis, protein folding and protein degradation Knockdown of these genes is expected to increase intracellular levels of damaged and denatured...

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Báo cáo khoa học: Puriﬁcation and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

Ngày tải lên : 30/03/2014, 04:20

... analysis of the puriﬁed hCYP11B1 (A) The puriﬁed hCYP11B1 was applied on RP-HPLC analysis using POROS R1 ⁄ 10 (2.1 · 100 mm; Applied Biosystems) as described in Experimental procedures The protein ... from h11B1 and that at 15.5 contained the apoprotein The apoprotein peak was collected and subjected to MALDI-TOF MS to verify the integrity of the protein moiety on Voyager DE-Pro (Applied Biosystems) ... volume and energetic properties of the binding of CO to hemoproteins Biophys J 66, 89–98 Tuckey RC & Kamin H (1983) Kinetics of O2 and CO Binding to adrenal cytochrome P- 450scc Effect of cholesterol,...

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Báo cáo khoa học: Identiﬁcation and functional characterization of an aggregation domain in long myosin light chain kinase ppt

Ngày tải lên : 30/03/2014, 04:20

... Elzinga M (1987) Phosphorylation of the 20,000-dalton light chain of smooth muscle myosin by the calcium-activated, phospholipiddependent protein kinase Phosphorylation sites and effects of phosphorylation ... C2-type Ig (Ig-C2) superfamily The Ig-C2 domains are found extensively in adhesion molecules and in intracellular cytoskeletal proteins, including titin, MyBP-Cm, MyBP-M, MyBPH, myotilin, and palladin ... (1993) Molecular cloning of chicken myosin-binding protein (MyBP) H (86-kDa protein) reveals extensive homology with MyBP-C (C-protein) with conserved immunoglobulin C2 and ﬁbronectin type III...

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Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt

Ngày tải lên : 30/03/2014, 08:20

... mammalian P2 Y receptor subtypes have been cloned and pharmacologically characterized Of these, P2 Y2 responds to ATP and UTP, whereas P2 Y1 and P2 Y12 respond to ADP P2 Y1 and P2 Y2 receptors are both coupled ... and P2 Y1 and P2 Y12 receptor protein expression and functional activity Examination of the effects of speciﬁc pharmacological agents (a single agonist and two speciﬁc antagonists of P2 Y1 and P2 Y12 ... measurable lipid phosphatase [phosphatase and tensin homolog (PTEN)] [3] PTEN dephosphorylates the 3¢ position of phosphoinositides and acts as an antagonist of PI3-K, resulting in a reduction of the concentration...

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Báo cáo khoa học: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis doc

Ngày tải lên : 31/03/2014, 09:20

... [26] using BamHI and XhoI sites, yielding pVT102-U-PtD6 To obtain pESC-LEUPSE1-PtD5, the ORF of pse1 was released from pY2PSE1 [20] using BamHI, cloned behind the galactose-inducible promoter PGAL1 ... EPA in P tricornutum and that the preferred route relied on intermediates of both the x6- and the x3-pathway (see Fig 1) The results presented in Table show that PtD 5p and PtD 6p are involved in ... synthesis of ARA or EPA (Table 2), respectively, PtD 5p and PtD 6p can function in both the x3- and x6-pathway Concerning selectivity, it was shown that neither PtD 5p nor PtD 6p showed any preference...

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Báo cáo y học: "Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments" pot

Ngày tải lên : 10/08/2014, 05:21

... lipoprotein receptors in modulating the in vivo metabolism of apolipoprotein Bcontaining lipoproteins in humans Clin Chem 1988, 34:B28 Greenow K, Pearce NJ, Ramji DP: The key role of apolipoprotein ... KH, Phillips MC, LundKatz S: Effects of polymorphism on the lipid interaction of human apolipoprotein E J Biol Chem 2003, 278:40723 Gregg RE, Brewer HB Jr: The role of apolipoprotein E and lipoprotein ... for apoE-(72-166) This tendency also positively correlates with the increasing concentration of DHPC Protein-lipid interactions and Protein-LDLR binding of ApoE-(72-166) Proteins To identify and...

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báo cáo khoa học: " Molecular and functional analyses of COPT/Ctrtype copper transporter-like gene family in rice" pdf

Ngày tải lên : 11/08/2014, 11:22

... COPT3 and COPT7 in shoot, and slightly suppressed COPT2 and COPT4 in root Zn deficiency induced COPT1, COPT5, and COPT7 and slightly suppressed COPT4 in root and induced COPT5, COPT6, and COPT7 in ... presence and absence of COPT6 MPY17 cells were co-transformed with p4 13GPD-COPT2GFP, p4 13GPD-COPT3-GFP, or p4 13GPD-COPT4-GFP and p4 16GPD (empty vector) or p4 16GPD-COPT6 and grown to log phase on ... COPT2-GFP COPT2-GFP + Vector + COPT6 COPT3-GFP COPT3-GFP + Vector + COPT6 COPT4-GFP COPT4-GFP + Vector + COPT6 Figure Localization of COPT2-GFP, COPT3-GFP and COPT4GFP in yeast MPY17 cells in the presence...

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báo cáo khoa học: " Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L" potx

Ngày tải lên : 12/08/2014, 05:20

... Example 5of comparison between absorption spectrum of the reaction product and authentic standard Example of comparison between absorption spectrum of the reaction product and authentic standard ... the lignin roadmap Current Opinion in Plant Biology 2002, 5:224-229 Hoffmann L, Maury S, Martz F, Geoffroy P, Legrand M: Purification, cloning and properties of an acyltransferase controlling shikimate ... and approved the final manuscript Acknowledgements We thank Prof G Mauromicale and Dr R Mauro of the University of Catania for providing plant material We are particularly grateful to Dr P Pinelli...

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Báo cáo y học: "Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats" pot

Ngày tải lên : 12/08/2014, 16:20

... http://respiratory-research.com/content/7/1/4 of NO2-exposed rats Cytokine and iNOS mRNA expression in AM subpopulations Figure Cytokine and iNOS mRNA expression in AM subpopulations of NO2-exposed rats ED7-positive and ED7-negative ... separation of AM subpopulations and performed in vitro cell stimulation and mediator analysis SS was responsible for animal preparation, performed mRNA expression analyses, and helped in FACS and ... expression of MMP-9 [42] but macrophage subpopulations were not investigated MMP-12 seems to play an important role in the development of emphysema at least in the mouse model since absence of this specific...

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Báo cáo y học: "Identification and functional characterization of cis-regulatory elements in the apicomplexan parasite Toxoplasma gond" pptx

Ngày tải lên : 14/08/2014, 21:20

... of the binding sites of the AP2-domain containing transcription factors as inferred from protein-based microarray studies conducted in P falciparum [28] In a study of the promoter strengths of ... Mutagenesis of both motifs within the UPRT promoter resulted in a seven-fold increase in reporter geneexpression, indicating that the two motifs function in repressing gene-expression and possibly possess ... R34 TRP-1 (RPA) TRP-1 (RPA) Mullapudi et al R34.8 TRP-2 (RPB) WT GCTTATATACG TGCATGCG MUT AAGGATGCGAG CAACACAC TRP-2 (RPB) (b) (d) -500 ATG RPS29 RPL38 RPS3 RPL13 RPL9 RPS25 RPS10 RPS13 0.9 p= 0.45...

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Biochemical identification and functional characterization of microrna target interactions in growth control and cancer transformation

Ngày tải lên : 09/09/2015, 10:17

... minimally including an Argonaute protein (green) and GW182 (blue) GW182 proteins contain an N-terminal AGO-binding domain, which provides multiple binding sites for Argonaute proteins and a bipartite ... Activated Protein Kinase miRNP microRNA associated ribonucleoprotein complexes mir-Q microRNA quantitative real time PCR PI(3 )P lipid phosphatidylinositol 3-phosphate RT Room temperature RTK Receptor ... training data came from IP, it is shown that mirWIP outperforms other popular prediction softwares including Targetscan, miRanda, PITA, PicTar and rna22 in recovering validated miRNA targets and...

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Molecular and morphological characterization of caudal neural tube defects in embryos of diabetic swiss albino mice

Ngày tải lên : 11/09/2015, 09:04

... 5-bromo-4-chloro-3-indoly phosphate bHLH Basic helix-loop-helix BLBP Brain lipid-binding protein BMPs Bone morphogenetic proteins BrdU 5-bromo-2’-deoxy-uridine BSA Bovine serum albumin cDNA Complementary ... The role of BMPs in the patterning of the dorsal aspect of the neural tube has been widely studied In the developing chick embryo, BMP4 and BMP7 induce dorsal cell types in neural plate explants ... by the expression of Isl1 (Helms and Johnson, 2003) 24 Chapter 1: Introduction 1.2.4.2.3 Other molecular factors 1.2.4.2.3.1 Brain Lipid-Binding Protein Brain lipid-binding protein (BLBP) is a...

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Functional characterization of giant killer in flower development and meristem regulation in arabidopsis thaliana 1

Ngày tải lên : 14/09/2015, 08:46

... NUBBIN OD optical density PBS phosphate buffered saline PCR polymerase chain reaction PFK PHOSPHOFRUCTOSE KINASE PHB PHABULOSA PHV PHAVOLUTA PI PISTILLATA PIN PINFORMED PMSF phenylmethylsulphonylfluoride ... found in chromosomal proteins and that binds to nuclear matrix attachment regions of DNA Overexpression and loss of function of GIK cause wide-ranging defects in patterning and differentiation of ... the regulation of reproductive organs patterning and differentiation in Arabidopsis thaliana through binding assay and expression analysis The GIK protein contains an AThook DNA binding motif that...

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Functional characterization of giant killer in flower development and meristem regulation in arabidopsis thaliana 2

Ngày tải lên : 14/09/2015, 08:46

... box indicates PPC domain (B) Schematic diagram of GIK genomic (upper panel) and predicted protein structure (lower panel) of GIK In the indicated CArG box, the uppercased sequence shows a typical ... are converted into sepals (in place of petals) and the third whorl organs are transformed into carpels (in place of stamens) Both AP3 and PI encode for members of MADS domain transcription factors ... SEP proteins to specify petals Whereas in whorl 4, the protein complex might be a combination of AG and SEP proteins (two molecules for each protein) in the specification of carpels 11 Figure 2:...

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