Inactivation and repair of escherichia coli following UV disinfection influencing factors and photolyase activity

... Background 88 4.2 UV Inactivation of E coli 90 4.3 Photoreactivation of E coli following UV Disinfection 94 4.4 Dark Repair of E coli following UV Disinfection 100 4.5 Comparison of Repair of Selected ... Photoreactivation of microorganisms after UV disinfection 50 2.9.4 Factors affecting photoreactivation 53 2.9.5 Dark repair 58 UV Disinfection and DNA Repair of Escherichia coli 59 2.10.1 UV disinfection of ... INACTIVATION AND REPAIR OF ESCHERICHIA COLI FOLLOWING UV DISINFECTION: INFLUENCING FACTORS AND PHOTOLYASE ACTIVITY QUEK PUAY HOON ELAINE (B Eng., NUS) A THESIS SUBMITTED FOR THE DEGREE OF...

PHOTOREACTIVATION OF ENTEROHEMORRHAGIC E. COLI, VRE AND P. AERUGINOSA FOLLOWING UV DISINFECTION

... apparent photoreactivation under sunlight following UV inactivation VRE exhibited apparent photoreactivation The dose of UV light required for 90% inactivation of VRE with and without photoreactivation ... Figure (Tosa and Hirata, 1999) VRE was the most UV resistant bacteria tested, while P aeruginosa and EHEC were weaker The UV doses required for 90% inactivation of VRE with and without photoreactivation ... - 20 - RESULTS AND DISCUSSION Photoreactivation of Pseudomonas aeruginosa The relationship between the survival ratio of VRE and visible light dose is shown in Figure Apparent photoreactivation...

Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the speciﬁcity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf

... evaluate the effects of ionic strength and Mg2+ on the activity and specificity of OGG1, we measured the apparent values of k2 and k3 under conditions of low salt (KPi only) and no Mg2+, low salt and ... Effects of ionic strength and divalent cations on the activity and specificity of Fpg and OGG1 The conditions inside a living cell differ from most buffer systems in which the activity and specificity ... may be affected by ionic strength, the concentration of divalent cations such as Mg2+, the nature of the buffering agents, the presence of competing polyamines and other small molecules, and crowding...

Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt

... with the sequences of 1CEV and 1RLA and pasted into the structural alignment The alignment was then corrected by hand Since the sequences of the two arginases and agmatinase greately differ in the ... that the environment of tryptophan residues is essentially conserved in the mutant enzyme On the other hand, the absence of major differences in the CD spectra of the wild-type and D153N agmatinases ... metal ligands are not substantially affected by the mutation, as revealed by the conservation of the whole topology of the active site, would explain the essentially unaltered interaction of the D153N...

Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx

... the C-terminal domain, the E coli Pta N-terminal domain is involved in stabilization of the hexameric native structure, in expression of the maximum catalytic activity, and in allosteric regulation ... Results Expression and puriﬁcation of E coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E coli Pta, three conserved domains can be detected ... of this enzyme and also the function of the DRTGG domain Taking into account the important role of Ptas, the results obtained in the present work, dissecting the different domains forming E coli...

ANTIBIOTIC RESISTANCE OF ESCHERICHIA COLI ISOLATED FORM POULTRY WORKERS, PATIENTS AND CHICKEN IN THE EASTERN PROVINCE OF SAUDI ARABIA ppt

... 1996) In conclusion, our data demonstrate alarmingly high individual and multiple resistance to antibiotics in E coli, reﬂecting the misuse of these agents in the poultry industry Since chicken ... Clinical Laboratory Standards (NCCLS 1993) In addition, E coli organisms isolated from chicken and Table Comparison of pattern of resistance of E coli isolates from chicken and patients E coli- chicken ... from patients (Table 4) Discussion We investigated the resistance of E coli isolated from poultry industry workers and patients to antimicrobial agents commonly used in the poultry industry and/ or...

Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot

... mode of the mixture of oligosaccharides prior to HPAEC, of the isolated main oligosaccharide of deacylated LPS and of acylated puriﬁed LPS from E coli F2513 (R4 core-type) In addition, the deacylated ... LPS (R4 core-type) is as depicted in Fig Structural analysis of E coli F653 core-oligosaccharides (R3 core) We have subjected puriﬁed LPS from E coli F653 to alkaline deacylation by hot alkali and ... core-oligosaccharides of E coli F653 (R3- core) and determined NMR chemical shift values MATERIALS AND METHODS Bacteria and bacterial LPS E coli strains 2513 and F653 were cultivated and used for the isolation of...

Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf

... with the amino group and the N3 atom of the cytosine (Fig 1) This study uses site-directed mutagenesis to further explore the contribution of these amino acids interacting with the pyrimidine ring ... consisting of three domains, the CORE, the LID and the NMPbind [1] A characteristic of bacterial CMP kinases is an extension of the NMPbind domain by 40 amino acid residues forming a three-stranded ... NMPs (A37 and T39 in these enzymes are equivalent to S36 in E coli CMP kinase) Substitution of R110 with Met in E coli CMP kinase affected both kcat and Km The kcat Km ratio with CMP and dCMP decreased...

Báo cáo Y học: The phosphotransferase system of Streptomyces coelicolor IIACrr exhibits properties that resemble transport and inducer exclusion function of enzyme IIAGlucose of Escherichia coli pptx

... hypothesis The demonstration of the IIACrr MalK interaction suggests that IIACrr may regulate the function of some of the > 140 MalK homologues found in the S coelicolor genome The one with the ... investigate is whether the mechanism of inducer exclusion is realized in S coelicolor Our observation that IIACrr could replace the inducer exclusion function of E coli IIAGlc by inhibition of maltose ... puriﬁcation of S coelicolor IIACrr and IIACrr( H72A) To study the function of IIACrr, we overexpressed three crr alleles in E coli encoding His-tagged IIACrr, native IIACrr, and native IIACrr( H72A) Therefore,...

Báo cáo khoa học: The pivotal regulator GlnB of Escherichia coli is engaged in subtle and context-dependent control potx

... intensity of the GlnB band was quantied as the sum of the concentrations of GlnB and GlnK and was denoted by PII* Note that PII* includes the modied forms of GlnB and GlnK as well, i.e GlnBUMP and ... bars indicate the SEM The dotted line is a result of two linear regression ts of the data points The extra abscissa on top of the gure indicates the molar ratio of PII*-trimer ATase-monomer The ... with the puzzle of a functional explanation for the existence of GlnB: why should control by PII* be absent altogether, and what then is the function of the cascade and of its pivot GlnB? The...

Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx

... small compared with those of G270V, E406G, and R166G Changes at the surface of the protein not appear to be major contributors to the solubility of the AMPP fragments The AMPP protein appears to have ... recombinant E coli AMPP and its domain variants Creating a library for truncated AMPP fragments The 1.3 kb pepP gene encoding E coli AMPP was PCR ampliﬁed from plasmid pPL670 [2] using a forward primer ... ZnCl2 inhibits the activity of AMPP [1] Here, the presence of ZnCl2 in the dialysis buffer led to the precipitation of each of the three proteins Neither the intact protein nor the FEBS Journal...

Báo cáo khoa học: Deﬁning the QP-site of Escherichia coli fumarate reductase by site-directed mutagenesis, ﬂuorescence quench titrations and EPR spectroscopy doc

... and D), and therefore HOQNO binding cannot be detected by its perturbation of the EPR spectrum of the oxidized enzyme (see below) In contrast to the results of Hagerhall et al [20], the ¨ ¨ EPR ... understanding of the effects of the mutants on the physiological quinol oxidation reaction catalyzed by FrdABCD, we studied their effects on the steady-state kinetics of the quinol: fumarate oxidoreductase ... because of the location of FrdB-T205H with respect to the QP site, the [3Fe-4S] cluster and the interface between FrdB and the membrane anchor subunits With the exception of the FrdC-W86R mutant, the...

Báo cáo khoa học: Properties and signiﬁcance of apoFNR as a second form of air-inactivated [4Fe-4S]ÆFNR of Escherichia coli pot

... residues reacted with DTNB (Table 1) The residues 4261 Disulﬁdes of apoFNR Fig MALDI-TOF spectra of aerobically (A) and anaerobically (B) prepared and carboxymethylated apoFNR The samples of apoFNR ... Therefore the redox state of the cysteinyl residues of the various forms of apoFNR – ‘aerobic’ and ‘anaerobic’ apoFNR, and apoFNR obtained by air-induced inactivation of [4Fe-4S]ÆFNR – was determined ... signal The MALDI-TOF spectrum of anaerobic apoFNR consisted of one major signal at 28 408 Da after alkylation equivalent to ﬁvefold alkylated apoFNR, and a minor signal of threefold alkylated FNR...

Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identiﬁcation of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx

... inactivation of wild-type CTPS by trypsin (A) Inactivation of CTPS-catalysed NH3-dependent CTP formation in the absence of ligands (s) and in the presence of ATP (10 mM, n), UTP (10 mM, h), and ... mutant CTP synthases The values of apparent kcat for the hydrolysis of glutamine by K187A (d) and R429A (s) apparent CTP synthases are shown Inset: values of kcat for the hydrolysis of glutamine ... parameters kcat and Km for CTP formation were determined with respect to NH3 and glutamine for each of the mutant enzymes except for K187A CTPS which was inactive (Table 3) Direct examination of the conversion...

Báo cáo Y học: Investigations into the mechanisms used by the C-terminal anchors of Escherichia coli penicillin-binding proteins 4, 5, 6 and 6b for membrane interaction ppt

... anchoring by E coli PBPs 4, 5, and 6b (Eur J Biochem 269 ) 5827 Fig Helical wheel representations of protein segments The C-terminal sequences of PBP4, PBP5, PBP6 and PBP6b, and the primary sequence of ... 0 .65 0.23 Ó FEBS 2002 Membrane anchoring by E coli PBPs 4, 5, and 6b (Eur J Biochem 269 ) 5823 The peptides P4 and P5 (Table 1) were supplied by PEPSYN, University of Liverpool, UK, produced by ... phase ﬂuidity and a decrease in liquid crystalline phase ﬂuidity (C) Here, we analyzed the C-terminal sequences of PBP4, PBP5, PBP6 and PBP6b to identify candidates with the potential to form oblique...

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Từ khóa: mara soxs and rob regulons of escherichia coli co transduction and location of escherichia coli genetic markers rnases i ii and iv of escherichia coli genes serobiotypes and antibiotic resistance proﬁle of escherichia coli strains isolated from aquaculture and other sources rnase i of escherichia coli rnase ii of escherichia coli rnase iv of escherichia coli expression purification of and monoclonal antibodies to s factors from escherichia coli laparoscopic repair of wide and deep uterine scar dehiscence following cesarean section flexibility and function of the escherichia coli trp repressor expression of human vasopressin and oxytocin receptors in escherichia coli inspection evaluation and repair of hydraulic steel structures surgical repair of the esophagus and stomach non destructive evaluation and quality control for bonded composite repair of metallic aircraft structures maintenance and repair of machinery components Báo cáo quy trình mua hàng CT CP Công Nghệ NPV Nghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt nam Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoại Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit ĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN Trả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ long Nghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chế Nghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúng Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tin Chuong 2 nhận dạng rui ro Tổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vật Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vật Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam MÔN TRUYỀN THÔNG MARKETING TÍCH HỢP