genes serobiotypes and antibiotic resistance proﬁle of escherichia coli strains isolated from aquaculture and other sources

Báo cáo khoa học: Broad antibiotic resistance proﬁle of the subclass B3 metallo-b-lactamase GOB-1, a di-zinc enzyme potx

Ngày tải lên : 14/03/2014, 23:20

... of E coli and contained signiﬁcant amounts of zinc and iron (0.45–0.75 iron ⁄ GOB-18 and 0.01–0.20 zinc ⁄ GOB-18) Only the mono-zinc form of GOB-18 could be obtained by 1258 remetallization of ... wild-type and mutant enzymes measured by ESI-TOF MS and calculated from their amino acid sequences The calculated difference between measured masses of the denatured and native wild-type and mutant ... substrates but meropenem and cefoxitin Q116H showed similar kcat and KM values in the presence of 50 lm zinc Table Summary of zinc binding for wild-type and mutants GOB1 Standard deviation values...

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Tài liệu Báo cáo khoa học: Metabolic ﬂux proﬁling of Escherichia coli mutants in central carbon metabolism using GC-MS docx

Ngày tải lên : 20/02/2014, 23:20

... aspects of functionality and regulation Nature 420, 190–193 44 Bachmann, B.J (1996) Derivations and genotypes of some mutant derivatives of Escherichia coli K-12 In Escherichia coli and Salmonella: ... mapping, and expression of the fumarase gene of Escherichia coli K-12 J Bacteriol 153, 588–596 31 Model, P & Rittenberg, D (1967) Measurement of the activity of the hexose monophosphate pathway of ... ﬂux proﬁle of the Mae/Pck mutant (Fig 6) This result was expected, as the fractions of pyruvate originating from malate and PEP originating from OAA that are indicative of in vivo malic enzyme and...

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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot

Ngày tải lên : 08/03/2014, 09:20

... addition isolated the complete core-oligosaccharides of E coli F653 (R3-core) and determined NMR chemical shift values MATERIALS AND METHODS Bacteria and bacterial LPS E coli strains 2513 and F653 ... in the negative ion mode of the mixture of oligosaccharides prior to HPAEC, of the isolated main oligosaccharide of deacylated LPS and of acylated puriﬁed LPS from E coli F2513 (R4 core-type) ... of anomeric protons and two pairs of signals originating from 3-deoxy protons characteristic of aKdop The assignment of H and 13C-resonances (Tables and 3) and determination of vicinal coupling...

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ANTIBIOTIC RESISTANCE OF ESCHERICHIA COLI ISOLATED FORM POULTRY WORKERS, PATIENTS AND CHICKEN IN THE EASTERN PROVINCE OF SAUDI ARABIA ppt

Ngày tải lên : 08/03/2014, 09:20

... Standards (NCCLS 1993) In addition, E coli organisms isolated from chicken and Table Comparison of pattern of resistance of E coli isolates from chicken and patients E coli- chicken isolates E coli- patient ... (1997) Prevalence of bacterial resistance to quinolones and other antimicrobials among avian Escherichia coli strains isolated from septicemic and healthy chickens in Spain Journal of Clinical Microbiology ... poultry and its possible impact on human health Our aims were to investigate and compare the patterns of resistance of E coli isolated from poultry industry workers, patients and chicken, and to...

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Báo cáo khoa học: Expression proﬁle of PIN, AUX ⁄ LAX and PGP auxin transporter gene families in Sorghum bicolor under phytohormone and abiotic stress pot

Ngày tải lên : 29/03/2014, 09:20

... distinct tandem duplicate gene clusters represented 10 SbPGP genes: two clusters with two tandem genes (SbPGP19–20 and SbPGP23–24), and another two clusters containing three tandem genes (SbPGP5–7 and ... AtLAX1 and two members in sorghum and rice (SbLAX1, -2, -4 and OsLAX2, -4, -5) and the other clade contains AtLAX2 and AtLAX3, and three members of SbLAX and OsLAX family Phylogenetic analysis of ... level of genes after treatment, compared with the expression of genes in leaves and roots planted in Murashige and Skoog medium (A–E) show expression of SbPIN, SbLAX and SbPGP genes under IAA and...

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Báo cáo sinh học: "Sources of variation and genetic proﬁle of spontaneous, out-of-season ovulatory activity in the Chios sheep" potx

Ngày tải lên : 14/08/2014, 13:21

... spontaneous, out -of- season ovulatory activity for over consecutive years The objective of this study was to examine the sources of variation and the genetic proﬁle of spontaneous, out -of- season ovulatory ... expensive and their effect disappears when the treatments end Furthermore, they are not consistent with consumer demands for the reduction of hormones, antibiotics and other substances Another approach ... month of the previous lambing class; = random effect of the lth ewe (Variance = Va ); Spontaneous out -of- season ovulatory activity in sheep pe e 69 = random effect of the permanent environment of...

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Báo cáo khoa học: A proﬁle of the residues in the second extracellular loop that are critical for ligand recognition of human prostacyclin receptor pdf

Ngày tải lên : 23/03/2014, 07:20

... properties of the side chains of the amino acids important for ligand-binding activity in receptor– ligand recognition, two additional mutants (Q162E and Q162L) were designed The side chains of Leu and ... conclusion, a proﬁle of the residue functions in human IP eLP2 was obtained by Ala scanning mutagenesis G159, Q162 and C165 within the N-terminal segment of IP eLP2, and L172, R173, M174 and P179 ... of polymerase (New England Biolabs, Beverly, MA, USA), 0.4 lm each primer, and lL of human lung cDNA, for 30 cycles of 98 °C for min, 60 °C for min, and 72 °C for The ampliﬁed products were isolated...

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Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the speciﬁcity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf

Ngày tải lên : 18/02/2014, 18:20

... of ionic strength and Mg2+ on the activity and specificity of OGG1, we measured the apparent values of k2 and k3 under conditions of low salt (KPi only) and no Mg2+, low salt and 20 mm Mg2+, and ... of organic acids, amino acids, inorganic phosphate and nucleic acids [27] To explore the dependence of the activity and specificity of Fpg and OGG1 on general ionic strength and the presence of ... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KCl and MgCl2 (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of OGG1 The extent of cleavage...

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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt

Ngày tải lên : 21/02/2014, 01:21

... positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and D153N mutant forms of E coli ... superimposition of the structures of B caldovelox arginase (red), rat liver arginase (yellow) and the modelled structure of E coli agmatinase (blue) Note the shorter extension, in the case of E coli agmatinase, ... ligand-manganese distances RESULTS AND DISCUSSION By site-directed mutagenesis, a D153N mutant form of E coli agmatinase was obtained The mutant enzyme retained about 5% of wild-type activity and...

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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx

Ngày tải lên : 06/03/2014, 11:20

... grants from CONICET and ´ ´ Agencia Nacional de Promocion Cientı´ ﬁca y Tecnologica M F Drincovich and C S Andreo are members of the Researcher Career of CONICET, and V A Campos-Bermudez and F ... compilation ª 2010 FEBS 1959 Escherichia coli phosphotransacetylase V A Campos-Bermudez et al Fig Comparative CD spectra of E coli Pta and truncated Ptas CD spectra of Pta, Pta-F1 and Pta-F2 were recorded ... E coli Pta and the three truncated Ptas in both the forward and reverse reactions (Fig 5A) NADH and ATP substantially inhibited the activity of E coli Pta in both directions (Fig 5A) On the other...

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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf

Ngày tải lên : 16/03/2014, 10:20

... Overproduction and molecular characterization of the modified variants of E coli CMP kinase The wild-type and various modified forms (S36A, R110M, D132A, D132H, D132N, D132S and R188M) of E coli CMP ... a dipole from the distal point of the rest of the protein Such a dipole will have weak interactions with the rest of the protein Thus, if turned off (by removal of Arg and protonation of Asp185), ... catalysis of E coli CMP kinase Substitution of the side chain from a well-structured protein can have two types of consequence: (a) a purely localized effect of binding due to removal of a specific...

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Báo cáo Y học: The phosphotransferase system of Streptomyces coelicolor IIACrr exhibits properties that resemble transport and inducer exclusion function of enzyme IIAGlucose of Escherichia coli pptx

Ngày tải lên : 17/03/2014, 23:20

... IIAGlc of E coli senses C-regulatory signals from both PTS and non-PTS carbon sources and responds via its phosphorylation state [9,13] It would be of great interest to examine whether IIACrr of ... histidine 75 and the experimentally proven phosphorylation site histidine 90 of E coli IIAGlc [27,28] Overexpression and puriﬁcation of S coelicolor IIACrr and IIACrr(H72A) To study the function of IIACrr, ... complementation of the glucose-speciﬁc PTS of E coli measuring phosphoenolpyruvate-dependent phosphorylation of methyl [a-14C]glucoside ([14C]aMG; Amersham) in the presence of E coli LM1 cell extract...

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Báo cáo khoa học: The pivotal regulator GlnB of Escherichia coli is engaged in subtle and context-dependent control potx

Ngày tải lên : 23/03/2014, 04:21

... the GlnB band was quantiﬁed as the sum of the concentrations of GlnB and GlnK and was denoted by PII* Note that PII* includes the modiﬁed forms of GlnB and GlnK as well, i.e GlnB–UMP and GlnK–UMP ... transient uridylylation of PII* and the transient deadenylylation of GS–AMP See also Figs and Cells had been grown in the presence of ammonia Uridylylation of PII* and deadenylylation of GS–AMP were measured ... Scanning of the autoradiograms and the determination of intensity of the PII* and PII*–UMP bands were as described above Some gels did not result in a complete separation of the uridylylated and native...

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Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx

Ngày tải lên : 23/03/2014, 07:20

... genes of mutants that confer increased TMP resistance are isolated and shufﬂed, and the new mutant library is monitored for increasingly higher levels of TMP resistance After several rounds of evolution, ... Kinetic and crystallographic analysis of mutant Escherichia coli aminopeptidase P: C-terminal domain of E coli aminopeptidase P insights into substrate recognition and the mechanism of catalysis ... fragments of the same length ) all were derived from the AMPP#2 fragment (Fig 1) Most of the mutant genes contained multiple mutations, two of which involved metal-binding ligands The D271N and E406G...

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Báo cáo khoa học: Deﬁning the QP-site of Escherichia coli fumarate reductase by site-directed mutagenesis, ﬂuorescence quench titrations and EPR spectroscopy doc

Ngày tải lên : 23/03/2014, 13:20

... structure of FrdABCD: T205, F206, Q225 and K228 from FrdB; R28, E29, W86, L89 and A93 from FrdC; and W14, F17, G18, H80, R81 and H84 from FrdD [3,4,10] Site-directed mutants of some of these residues ... behavior of wild-type and a subset of the mutants of FrdABCD in DW35 membranes The wild-type enzyme has a Km for LPCH2 of approximately 225 lm and a kcat of approximately 71 s)1 The FrdB-T205H and ... spin-coupled pair of [4Fe-4S] clusters and comprises a peak at g ¼ 2.29, and troughs at g ¼ 1.87 and 1.67 Addition of HOQNO causes the appearance of a peak at g ¼ 1.98 (compare Figure 4Ci and ii) Overall,...

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Báo cáo khoa học: Properties and signiﬁcance of apoFNR as a second form of air-inactivated [4Fe-4S]ÆFNR of Escherichia coli pot

Ngày tải lên : 23/03/2014, 15:20

... of the cysteinyl residues in apoFNR and in reconstituted [4Fe-4S]ỈFNR ApoFNR that was devoid of Fe and acid-labile sulfur (not shown) was obtained by isolation of FNR from E coli under oxic and ... redox state of the cysteinyl residues of apoFNR should be relevant for function and properties of the protein Therefore the redox state of the cysteinyl residues of the various forms of apoFNR – ‘aerobic’ ... fivefold alkylated apoFNR, and a minor signal of threefold alkylated FNR (Fig 1B) Therefore, aerobic and anaerobic apoFNR are mixtures of FNR with one, three and five, and five and three cysteine...

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Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identiﬁcation of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx

Ngày tải lên : 23/03/2014, 17:22

... function of the time of incubation The ability of various ligands to protect both wild-type and mutant CTPS from proteolysis was examined using ATP (0.5, 1.0, 2.0, and 10 mM), UTP (0.5, 1.0, 2.0, and ... masses of % 25 and % 28 kDa In the presence of ATP and UTP (10 mM each) (B), and CTP (2.5 mM) (C), only production of the 10- and 53-kDa cleavage fragments is observed In the presence of GTP ... inactivation of wild-type CTPS by trypsin (A) Inactivation of CTPS-catalysed NH3-dependent CTP formation in the absence of ligands (s) and in the presence of ATP (10 mM, n), UTP (10 mM, h), and ATP and...

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Báo cáo Y học: Investigations into the mechanisms used by the C-terminal anchors of Escherichia coli penicillin-binding proteins 4, 5, 6 and 6b for membrane interaction ppt

Ngày tải lên : 31/03/2014, 08:20

... sequences of the penicillin-binding protein and genes of Escherichia coli Nucleic Acids Res 16, 1617 Schiﬀer, M & Edmundson, A.B (1967) Use of helical wheels to represent the structures of proteins and ... stretching) and amide II (predominantly N–H bending) Figures and show spectra for P4 and P5 absorbance in the spectral range of the amide I band and from these spectra, the relative levels of peptide ... subtraction of baselines resulting from neighboring bands Similar to the deconvolution technique, the bandshapes of the single components are superpositions of Gaussian and Lorentzian bandshapes...

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Báo cáo khoa học " Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake " ppt

Ngày tải lên : 28/06/2014, 11:20

... absorbance in the presence of the sample and H2 O2 , A0 is the absorbance of the control in the absence of the sample and H2 O2 , and A is the absorbance only in the presence of the H2 O2 2.6 Nuclear ... described and analyzed by GC–MS 2.5 UV and infrared (IR) spectra analysis TMP-A was tested in UV from 200 to 600 nm and infrared analysis of the samples was obtained by grinding a mixture of polysaccharide ... spectra of polysaccharide TMP-A Results and discussion 3.1 Extraction, purity and composition of polysaccharides The crude polysaccharide, named TMP, was obtained from the fruiting bodies of T matsutake...

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Báo cáo vật lý: "Immobilization of Dithizone onto Chitin Isolated from Prawn Seawater Shells (P. merguensis) and its Preliminary Study for the Adsorption of Cd(II) Ion" pdf

Ngày tải lên : 07/08/2014, 14:20

... varied in the range of 0.05–0.5 g and the pH of the solution was kept constant at 6.0 RESULTS AND DISCUSSION 3.1 Isolation and Characterization of Chitin Isolation of chitin from prawn seawater ... structure of (a) chitin and (b) dithizone EXPERIMENTAL 2.1 Reagents and Materials Metal salts of analytical grade and dithizone (1,5-diphenylthiocarbazone) of reagent grade, were all purchased from ... reference and (b) isolated chitin Table 1: Ash and total nitrogen contents of isolated chitin from prawn shell Parameters Content (% wt) Total nitrogen Ash content 3.2 3.9 0.59 Immobilization of Dithizone...

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