expression purification of and monoclonal antibodies to s factors from escherichia coli
Báo cáo khoa học: "The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)" pdf
Ngày tải lên :
07/08/2014, 15:20
... Davis et al resistance to diseases of economic importance to cattle such as anaplasmosis and babesiosis [25,25,26] To evaluate the immune responses of water buffalo to infectious agents and potential ... frequency of lymphocyte subsets was similar to that observed in cattle As in cattle, the WC1+ population of γδ T cells was comprised of subsets that express the WC1-N3 and WC1-N4 isoforms The frequency ... [8] mAbs that identify these molecules should prove useful in study of the response to infectious agents and vaccines [8] ACT1 is expressed within 16 to 24 hrs of stimulation with A similar to the...
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Báo cáo y học: "The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin" ppsx
Ngày tải lên :
09/08/2014, 06:22
... B3(Arg27aSer) B3 B3a 2.5–4 IS4VHi IS4 IS4 IS4 50–56 IS4VHii IS4 IS4 IS4 65–70 IS4VHiii IS4 IS4 IS4 48–90 IS4VHiv IS4 IS4 IS4 48–90 IS4VHx IS4 IS4 IS4 78–94 IS4VHi&ii IS4 IS4 IS4 74–80 IS4VHi B3 ... antibodies [29] This system was used to test the binding properties of combinations of heavy chains and light chains derived from a range of human antibodies One of these antibodies, IS4, is an IgG ... All of these light chains contain numerous somatic mutations Previous statistical analysis has shown that the observed pattern of replacement mutations in the CDRs of these sequences is consistent...
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Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx
Ngày tải lên :
17/03/2014, 09:20
... (NaCl/Tris), pH 8.0 The flow rate used was 0.5 mL per minute and 0.5 mL fractions were collected Fractions were chosen and pooled based on analysis of silver staining after SDS/PAGE and stored at ... the two studies, a recombinant construct consisting of CCP1 from the C4BP b-chain and CCP2 from the C4BP a-chain was fused to the modified tPA [14] The results of this exercise again suggested b-chain ... F114A/I116 A, and H108A As it is not possible to mutate all the residues of the CCP2 to investigate the protein S C4BP interaction, we reasoned as follows (a) Residues R60 and R101 point away from the...
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Báo cáo y học: " Presence and utility of IgA-class antibodies to cyclic citrullinated peptides in early rheumatoid arthritis: the Swedish TIRA project" docx
Ngày tải lên :
09/08/2014, 10:23
... physician 's global assessment of disease activity were registered Functional disability was also assessed at baseline and after 12, 24 and 36 months using the Swedish version of the Health Assessment ... patients (14% versus 12%) for both IgA-class and IgG-class antibodies IgA anti-CCP status and IgG anti-CCP status versus smoking and shared epitope Forty-three per cent of the current smokers were ... without a SE A much weaker (nonsignificant) trend was recorded regarding IgA anti-CCP status versus SE status (39% in SE+/+, 27% in SE+/-, and 21% in SE-/-) Discussion Analysis of IgA-class autoantibodies...
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Báo cáo khoa học: " Detection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressed antigens" pptx
Ngày tải lên :
12/08/2014, 04:20
... proteins In addition, yeast combines the simplicity and costeffectiveness of bacterial expression systems and is better than the more expensive and less convenient cell culture systems [22] To date, ... reports of using yeast expressed AIV recombinant proteins as antigens for ELISA To the best of our knowledge, this is the first report of expressing NP, N3 and N7 proteins of AIV in S cerevisiae and ... Laboratory, GA USA, and Synbiotics, Inc., CA USA) and the rest were positive for Newcastle disease (ND), infectious bursal disease (IBD) and infectious bronchitis (IB) obtained from Synbiotics and...
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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
Ngày tải lên :
09/09/2015, 08:16
... (Vasilakis et al., 2008) IV V Southeast Asian / American Sylvatic I Genotype I Southeast Asia, China, East Africa Thailand in 195 0s to 196 0s Sylvatic strains from Malaysia West Pacific islands and ... and post-translationally modified by proteases from both the host and virus into structural (E, C and prM) and NS proteins (NS1 NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) NS proteins then initiate ... containing prM and 80% of E protein (80E) from each of the four DENV serotypes, to be stably transformed into Drosophila melanogaster Schneider (S2 ) cell expression system for the expression of E protein...
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Cellular gene expression profiles of human macrophages exposed to chlamydia pneumoniae and treated with low density lipoprotein
Ngày tải lên :
03/10/2015, 11:36
... first stages of heart disease are lesions and cracks forming in the blood vessel walls normally at the points of highest pressure or stress (near the heart) The second stage is the body trying to ... the associated field is transcriptomics Transcriptomics refers to the genome-wide study of mRNA expression levels, and will be one of the approaches used in my research study 16 Survey of Literature ... itself by depositing fatty substances (cholesterol, lipoproteins) inside the blood vessels to fill the cracks These fatty substances can begin to build up and clog the blood vessels causing stroke...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
Ngày tải lên :
20/02/2014, 23:20
... has been used to describe the results of fast kinetic studies [15] and investigations with dynamic light scattering and time-resolved uorescence anisotropy measurements [30] In these studies, ... possesses a unique structure and activity [2] The reinterpretation of this paradigm was proposed by Passner & Steitz [6] on the basis of the crystal structure of the CRPcAMP complex, and it has ... Edinburgh Instruments Results Fluorescence labeling of CRP Each subunit of CRP possesses three cysteine residues, two in the N-terminal domain (Cys19 and Cys92) and one in the C-terminal domain (Cys178)...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
Ngày tải lên :
21/02/2014, 01:21
... Construction of a homology model of SmGSTO SmGSTO structure was built using the human GSTO 1–1 structure as template in the SWISS-MODEL modeling Ó FEBS 2002 Omega-class GST from Schistosoma mansoni ... regions of SmGSTO presented significant divergences from the known GSTO, at the primary structure level Indeed, the GSTO class represents a particular class of the GST superfamily which possesses specific ... experiments (see below) Expression of SmGSTO The recombinant SmGSTO was used to produce polyclonal antibodies in immunized rabbits Expression of SmGSTO along the parasite life cycle was analyzed by Western...
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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
Ngày tải lên :
07/03/2014, 16:20
... Iodide was used as a quencher The upper panel represents a plot of SternVolmer constants as a function of the emission wavelength The lower panel shows the FQRS spectra: ( ) the total emission spectrum ... wavelength was 295 nm, so it can be assumed that the uorescence emission observed was only from tryptophan residues A typical SternVolmer plot of uorescence quenching of the single tryptophan of the ... conformational changes in the protein upon binding of cAMP and fragments of DNA possessing specic sequences Each CRP wild-type (CRPwt) subunit contains two tryptophan residues at positions 13 and 85, both...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
Ngày tải lên :
17/03/2014, 03:20
... pressure to maintain the arrangement of these exons In mammals, hypoxic stress is known to increase GLUT1 and GLUT3 expression in specific tissues to enhance the uptake rate of glucose both to ... analyses and homology searches were performed using the online BLAST suite of programs (NCBI, USA) Statistical analysis A nonparametric v2 test was used to test the hypothesis that the ratio of expression ... 2590 Discussion Fig In vivo expression and response pattern to hypoxia of gcGLUT A representative Northern blot derived from the tissues of one normoxic and one hypoxic fish from a total of four...
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Báo cáo y học: "Destructive effects of murine arthritogenic antibodies to type II collagen on cartilage explants in vitro" pps
Ngày tải lên :
09/08/2014, 06:23
... fixation and processing as described above Statistical analysis Statistical analyses were performed using Statistica for Windows, Version 4.5 (Statsoft Inc., Tulsa, OK, USA) ANOVA was performed to ... auto-select tool was used to designate and create a line at the point that the loss of staining ended; using the two-point straight-line measurement tool, the distance of loss was measured from ... last 10 years as a most effective means of identifying and quantifying differences between defined areas or single points of histological specimens, and the present study illustrates its use...
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Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf
Ngày tải lên :
16/02/2014, 14:20
... partners, either lipids from the cytoplasmic membrane or proteins such as chaperones or components of the Tat translocase [13,14] Such a situation is encountered in the signal peptide of Sec substrates, ... previously missing signals remained absent in the 1H,15N-HSQC spectrum of NarJT These observations render it impossible to solve the structure of both NarJ and NarJT by NMR 2D H,15N-HSQC NMR spectra ... peptides used in this study were chemically synthesized and purified by Synprosis (Marseilles, France) The molecular mass of each peptide was verified by mass spectrometry NMR experiments for NarG...
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Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt
Ngày tải lên :
18/02/2014, 16:20
... amino acid residues involved in dTTP regulation of dCTP deaminase The design of the mutant enzymes H121A and V122G was inspired by the results from analysis of crystal structures as discussed later ... binding of the substrates In this study, we present results from structural and mechanistic studies on dTTP inhibition of E coli dCTP deaminase Coordinated closure of the active site and rearrangement ... moved to new positions The significance of this last observation is that due to the proximity of residues 120–125 from each subunit in the centre of the trimer, the side chains of Val122 of one subunit...
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Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc
Ngày tải lên :
19/02/2014, 16:20
... spectral absorption maxima of these complexes Despite subtle differences in the spectral maxima in the visible region of the Fe(III) species, spectra of the Asp40 mutants were essentially similar to ... value Results Optical absorption spectra Figure depicts the optical absorption spectra of Fe(III), Fe(II) and Fe(II)-CO complexes of the Asp40Ala and Asp40Asn mutants Table summarizes the spectral ... is in contrast with data on mutants of Met95 at The present study reveals an interesting aspect of the structural and functional relationships of Ec DOS Mutations at Asp40 did not essentially affect...
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Tài liệu Báo cáo khoa học: Quantitative estimation of channeling from early glycolytic intermediates to CO2 in intact Escherichia coli pdf
Ngày tải lên :
19/02/2014, 18:20
... the complex proposed by Mowbray and Moses [18] on the basis of observations using E coli extracts Observations by Clegg and Jackson [16] support the conclusion that channeling of several glycolytic ... this example) [14C]Glucose is converted to [14C]Glu6P as it enters the cell via the phosphotransferase system (PTS) and thence to [14C]Fru6P by E2 (phosphoglucoisomerase; PGI) In the absence of ... channeling is being assessed by measuring the isotopic composition of a downstream compound (such as CO2), the signature of channeling will be lost if any step between the reaction being considered and...
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Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
Ngày tải lên :
21/02/2014, 00:20
... release The crystal structure and the reaction mechanism of APase from E coli, suggested by Kim and Wyckoff [1], as well as the high resolution crystal structure determined by Stec et al [10], offers ... using mM pNPP as substrate cooperativity that possibly exist between the subunits Both phosphate-binding and calorimetric studies suggested positive cooperativity of Zn2+ binding to the M1 sites ... deviations are apparently reduced as an increased Vm reduces the slope of the curve, making the deviations less obvious Analysis was performed by normalization of all curves to the same Vm to verify...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
Ngày tải lên :
21/02/2014, 01:21
... mutations was confirmed by dideoxy DNA sequencing of the entire SOD-coding region of each expression construct (results not shown) Expression of pGH-SOD derivatives In pGHX(–), SOD gene expression is ... SODs DISCUSSION The basis for metal cofactor selectivity in vivo and metal ion reaction specificity in the homologous FeSODs and MnSODs has been difficult to ascertain by comparative analyses of ... and sidechains of residues relevant to mutation studies here are shown as ball and stick (FeSOD Q69, A141 and MnSOD G77, Q146) Hydrogen bonds and metal contacts between residues of FeSOD are shown...
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Báo cáo khoa học: High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli pot
Ngày tải lên :
07/03/2014, 05:20
... B shows CysM when releasing the products after the reaction has taken place Such a see-saw system is discussed as ‘half-site reactivity’ [21] We conclude that the observed asymmetry suggests ... Discussion CysM structures In solution, CysM from E coli is a dimer of · 32 893 Da consisting of 303 amino acid residues per subunit An earlier study [6] yielded a medium quality structure of ... that CysM is a suitable candidate for exploring the half-site reactivity hypothesis Experimental procedures Mutagenesis and activity assay Fig Relative B-factor distributions of CysM subunits in...
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Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot
Ngày tải lên :
07/03/2014, 16:20
... gels containing m urea and the activity of ribosomes was tested in poly(Phe) synthesis assays according to [38] pppGpp synthesis pppGpp synthesis assays were essentially carried out according to ... formation of such complexes stabilises SF and leads to the small production 688 Fig Ribosomes stimulate pppGpp synthesis by SF in the absence of added tRNA TC-ribosomes (1.7 lM, lanes 2, 4) or 5 0S (1.7 ... tRNA-dependence of pppGpp synthesis Binding states of tRNAs It was important to determine the binding states of tRNAs in ribosomal complexes that stimulated pppGpp synthesis by SF as tRNAs could either...
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