Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... 2011 FEBS 1789 Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis Airat Kayumov1,2, Annette Heinrich3, Kseniya ... blotting.Fig. 6. The interaction of truncated TnrA proteins with GlnK and GS. (A) BIAcore analysis of GlnK and GS binding to wild-type TnrA (TnrAwt), TnrA6 , TnrA2 0, and TnrA3 5. The analyte (40 nM GlnK orGS ... fully inhibitory, and 2mm Mg2+or Mn2+restored more than 50% of the GlnK TnrA interaction in the presence of 2 mm ATP.On the other hand, 2-oxoglutarate did not in uence the GlnK TnrA interaction, ...

Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

... analyzed the effect of HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actin In the first series of experiments we analyzed the effect of HSP25 ... The quantity of actin in the pellet was plotted against the totalquantity of actin in the sample.ResultsHeat denaturation of actinBefore starting the investigation of the HSP25–actin inter-action ... Effect of heating on the kinetics of polymerization (A) and salt-induced increase of the light scattering (B) of actin. Actin (15 lM)containing 10% or pyrene-labeled actin in buffer G were incubated...

Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... 17.6Fig. 5. Insertion of stefins into DOPC and DOPG monolayers. (A)Kinetic traces of the insertion into DOPG lipid monolayers at initialpressure of 5 mNÆm)1. The proteins were injected into the sub-phase ... signal increase during the injec-tion phase was the largest and there was low dissoci-ation after the end of injection.Discussion The main hypothesis for pathology in AD and otherneurodegenerative ... (TEM) and atomic forcemicroscopy [15]. In this study, we measured the interaction of stefin B with various combinations of phospholipid monolayers and bilayers. Interaction of stefin B in the prefibrillaraggregated...

Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx

... decreasedinteractions, and the ND49 mutant showed 80% loss of interaction with NV. These observations suggestthat there is a major interacting domain within the N-terminal 49 amino acids of the SeMV ... ELISA in the absence of the interactingproteins. rMP interacted with NV in both the experi-ments (Fig. 4A,B).Nature of the MP–NV interaction To determine the concentration dependence and nature of ... performed in triplicate usingconstructs in both the GAL4 activating and DNA bindingdomain fusion proteins. Expression of the interacting pro-teins was measured by direct antigen-coating ELISA. The cells...

Báo cáo khoa học: Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus ppt

... investigate the basis of the interaction of E2p with E3 in the assem-bly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearo-thermophilus. Thirteen of the 55 amino acids in the PSBD ... describes the interaction of a15N-labelledPSBD (residues 119–171) of the B. stearothermophilusE2p polypeptide chain with the dimer of a protein domain (E3int) representing the interface domain (resi-dues ... otherwise of the proposed E3-bindingsite is crucial to a proper understanding of protein protein interactions within the multienzyme complex.Further, an improved solution structure of PSBD wassubsequently...

Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf

... quantities of protein utilizing the principle o f protein dye binding. Anal. Biochem. 72, 248–254.35. Laemmli, U.K. (19 70) Cleavage of structural proteins us ing the assembly of the h ead of b acteriophage ... o ther inducible factor interacts with R LjunRP bound to the )148 to )124 region, and further enhances c-jun transcription in proliferatingliver.Most of the studies to gain an insight into the ... whether the increase in c-jun mRNA levels could b ecorrelated w ith the appearance of the factor, rRLjunRP,involved in the f ormation of complex C2, observed with rRNE-d, and if the time of...

Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule ﬂuorescence microscopy pdf

... C1r2–C1s2 in the C1 complex that hinders the interaction of the C1qsubunit with the polysaccharide. According to this hypo-thesis fucoidan should then interact with the hinge regioncontaining the binding ... determine the site of interaction on the protein. For this purpose we took advantage of the binding properties of C1q toward DNA and of an emergingtechnique allowing the molecular combing of DNA ... contains the binding site of DNA, wewondered whether the association of the tetramer C1r2–C1s2to C1q could interfere with the binding of C1q toDNA. We studied the binding to DNA of the...

Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx

... characteristic of the TÆT minor-groove than the AÆA minor-groove, in the binding of DAPI[16]. In conclusion, the main cause of the strong inhibitoryeffect of DAPI on DNA replication in vitro reported in ... ﬂuorescence and UV thermal melting studies. The addition of ATT-template to DAPI solution determinesdrug ﬂuorescence changes that are distinctive of the drugbinding into the minor-groove of the double-helix ... mechanism of the action of minor-groove binding ligands.ConclusionThis study reports the effect of the classical minor-groovebinding ligand DAPI on AATÆATT trinucleotide repeatreplication in vitro,...

Báo cáo khoa học: Interaction of the P-type cardiotoxin with phospholipid membranes pptx

... both1H-NMR and 31P-NMR data argue for the absence of CTII binding to Pam2Gro-PCho. The in uence of CTII binding to MLV of Pam2Gro-PGro on the lineshapes of the 31P-NMR spectra of the phospholipid ... inserted volume of CTIImolecule within the micelle and within the membraneincreases when the imidazole group of His31 becomesneutral. This is reﬂected in the pH-dependence of the ability of ... CTs in uence the bending elasticmodulus of the membrane and, hence, the transbilayerpressure proﬁle originating from the stiffening of lipidmolecules in the polar but not in the acyl region of the...

Báo cáo khoa học: Interaction of caspase-3 with the cyclic GMP binding cyclic GMP speciﬁc phosphodiesterase (PDE5a1) potx

... competence in PDE4 and to play a keyrole in the binding of competitive PDE4 inhibitors [36]. The side chain of this residue may conceivably p-stack with the purine base of the bound substrate and form ... on the other side of the catalytic pocket, identiﬁed by Turko and colleagues, such as Q765 [13]. The removal of part of the catalytic pocket explains the inactivity of the engine-ered protein truncated ... in PDE2A3,PDE4C,PDE4D,PDE6ab and PDE11A1. D778at P4 of the caspase-3 consensus site in PDE5A1 is replacedwithEinPDE11A1andPDE6ab, Q in PDE4C, R in PDE4D and S in PDE2A3. Therefore, the replacement of the 4D effectively...

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