... rapidly passed backfrom the R1E subunit to the tyrosyl of the R2F sub-unit. Subsequently, the dicysteine unit is re-reduced byan exogenous reductant and catalytic activity isrestored. By not ... catalysis, as expected.The tyrosyl radical of the R2F subunit was alsomonitored during the course of the enzyme assay.Curiously, under conditions where the product wasformed, the tyrosyl radical, ... 408 nm, characteris-tic of a tyrosyl radical (Y Æ), which is sensitive to the radical scavengerhydroxyurea. A novel enzyme assay revealed the direct involvement of Y Æin ribonucleotide reduction...
... activity. NPY is able to act throughfive different receptors (Y1 , Y2 , Y4 , Y5 and y6 ) thatvary in their binding profiles and in their distributionin the central nervous system and periphery. Y1 , Y2 and ... Cytokines, estrogen, and postmeno-pausal osteoporosis – the second decade. Endocrinology139, 2659–2661.LeptinNPY Y2 Y1 Y2 NPYNPYOsteoblastsHypothalamusFat tissueSympathic nervous systemNPYCirculating ... response ismediated centrally, the Y1 response is mediated byosteoblastic Y1 . Thus, hypothalamic signals sustain asystemic regulatory influence via Y2 , whereas osteoblas-tic Y1 enables additional...
... carboxypeptidase Y; FM4-64, N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenylhexatrienyl) pyridinium dibromide; GFP, greenfluorescent protein; IC, carboxypeptidase Y inhibitor; PC, phosphatidylcholine; ... Hayashida M, Fujii T, Hata Y, Hayashi R &Ueda M (2004) Crystallization and preliminary X-rayanalysis of carboxypeptidase Y inhibitor ICcomplexedwith the cognate proteinase. Acta Crystallogr ... carboxy-peptidase Y in yeast. Biochem Biophys Res Commun 60,1051–1057.3 Lenney JF, Matile Ph, Wiemken A, Schellenberg M &Meyer J (1974) Activities and cellular localization ofyeast proteases...
... ovary; cNPY, chicken neuropeptide Y; cPP, chicken pancreatic polypeptide; cPYY, chicken peptide YY; Hsa,Homo sapiens chromosome; pNPY, porcine neuropeptide Y; PP, pancreatic polypeptide; pPYY, ... > cPP (see Table 1). Interestingly, pNPY had alower affinity than pPYY, thereby making it unlikelythat cNPY would bind with higher affinity (they differby only two conservative replacements as ... determine theaffinity of cPYY also to the previously cloned Y- familyreceptors in chicken. Among the intact peptide ligands,the rank order of potency was pPYY > pNPY >cPYY > cPP (see Table...
... [24–27]. There are two types ofnongerm cell CSD proteins and these are called dbpB(also known as YB-1, MSY-1, chkYB-1b, EF1A, p50 andFRGY1) and dbpA (MSY4, chkYB-2 and YB2/RYBa).DbpB and dbpA ... [47],dbpB/YB-1 binding to the IL-2 5¢-UTR [31] and the VEGF 5¢-UTRCSD site 1. MSY1, ChkYB-1b and FRGY1 are dbpB/YB-1 proteins.MSY-4 and chkYB-2 are dbpA proteins, and MSY2 and FRGY2 aregerm ... of stably transfected cell lines was asdescribed previously [15]. Hypoxic conditions (1% O2)weregenerated in a hypoxic chamber (Edwards InstrumentCompany, Sydney, Australia).Analysis of...
... th y sản”,“Công nghệ chế biến tôm chua.”, “Công nghệ chế biến mắm ruc, mắm nêm”, 3. Ths. ,“CÔNG NGHỆ CHẾ BIẾN NƯỚC MẮM”, “Công nghệ chế biến th y sản”, ... CHẾ BIẾN NƯỚC MẮM”, “Công nghệ chế biến th y sản”, 4. “X y dựng quy trình sản xut chế phẩm vi khuẩn lactic ứng dng trong lên men tôm chua”, ... 4.4.2 Mm ruc Bng 4.6 ng và s c khi ch bin mm ruc Nguyên nhân + + không...
... commonly observed in adenylate-forming enzymes, such as aminoacyl-tRNA synthetases,Keywordsacyl-CoA synthetase; enantiomer; fire y luciferase; kinetic resolution; nonsteroidalanti-inflamatory drugsCorrespondenceD ... kinds of hydrolytic conditions, chemicalhydrolysis under high pH and enzymatic hydrolysisunder neutral pH, were examined, because there wassome fear of racemization by the chemical hydrolysis.The ... Identification of 2-arylpropanoyl-CoA produced by LUC-Hwith TOF-MS analysis. For the detection of 2-arylpropanoyl-CoAsby TOF-MS analysis, see the Experimental procedures section.Entry ThioesterPredicted[M-H]–Observed[M-H]–Error(p.p.m.)1...
... data). Tryptophan and tyro-sine residues (Y1 7, Y2 0, Y8 2, W84, and W369) in CPYwould be perturbed by the carbohydrate moiety, thusincreasing their fluorescence, since they are in close proximityto ... 2562–2567.Table 1. Summary of qualitative thermodynamic parameters for the temperature- and pressure-induced transitions of CPY, Dgly CPY, proCPY andDgly proCPY calculated by fluorescence analysis. Errors ... pro-nounced hydration upon unfolding of the unglycosylatedform.At high pressure, in the glycosylated forms the carbohy-drate moiety of CPY and proCPY would be hydrated tocompensate the volume contraction...
... 6110.Abbreviations:ATEE,N-acetyl-L-tyrosine ethyl ester monohydrate;BTEE, benzoyl-L-tyrosine ethyl ester; CPY, carboxypeptidase Y; GdnHCl, guanidine hydrochloride.*Present address: Sanyo Chemical Co., Kyoto, Japan.Present ... of carbohydratemoiety in carboxypeptidase Y: structural study of mutant enzymelacking carbohydrate moiety. Biochem. Biotechn Biosci. 63, 1045–1050.2. Jung, G., Ueno, H. & Hayashi, R. ... cleft of CPY is constructed with five a-helices,these locate as if they surround the catalytic triad. Inaddition, five disulfide bridges (Cys56–298, Cys198–207,Cys217–240, Cys224–233 and Cys262–268)...
... Gorrell11 Centenary Institute, Sydney Medical School, University of Sydney, NSW, Australia2 Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sydney, NSW, AustraliaKeywordsantiplasmin-cleaving ... pmolÆmin)1Ælg)1on benzyloxycar-bonyl (Z)-Gly-Pro-AMC and 1830 nmolÆmin)1Ælg)1onH-Gly-Pro-p-nitroanilide, respectively. To assay thesubstrate specificity of each protease, enzyme activityassays were ... megakaryocytes, adipose tissue and gut [45,46].Dipeptidyl peptidase truncation of NPY by FAP orDPP4 yields NPY3–36. NPY3–36is inactive on the Y1 receptor, and has greater affinity for the Y2 ...
... Pigs$\$\H$\N2))+u)3,u)m>=,)mN)",./C0$'8$'$''8$VIẾT BÁOCÁO KHOA HỌC TS. Lê Đình Phùng ĐẠI HỌC NÔNG LÂM HUẾ9:';<=>98985'8'?",./C0D+QERSF ... &)m>$N)j^)`m_ao xk()8]!" ;y " Y DVL]" Y H`@A& !7@FH`@A7@j$5'kFH`@A7!]=$c&$N@F$\HL)8]\&8 ... ,G8X)!""7#89:+ &1n"2" Tên Báocáo nói về cài gì?Tóm tắt Tóm tắt, có khả năng đứng độc lậpĐặt vấn đề Vấn đề nghiên cứu, cái...