Tài liệu Báo cáo khoa học: cN-crystallin and the evolution of the bc-crystallin superfamily in vertebrates pdf

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... N7 (Fig. 1C). The 2¢OH of the ribose is hydro-gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the riboseare involved in coordinating a putative ... solutions. The ligandbound was interpreted as GMP because the N2 of the base makes a hydrogen bond to a main chain car-bonyl. Binding of a xanthine base from XMP in the same position, would lead to the ... and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine.Table 2. Km and Vmaxvalues for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 ﬁlter...

Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf

... on the N-terminal FP, it wouldbe of interest to compare the structure of the internal FP and i ts interaction w ith membrane bilayer, including in particular the structural in uence of proline. ... into the target membrane in the fusion process[10,11]. In the following, a variety of physical properties of the putative IFP of ASLV are reported and differencesbetween N-terminal and internal ... rhodamine ﬂuorescence indicatesself-assembly of IFP–wt in the lipid bilayerTo further examine the organization of the fusion peptide in the membrane, the peptide was l abelled with rhodamine[26]....

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... E. coli and Synechocystis sp. bacteria further, togain insight into the function of these proteins and the evolution of the MAPEG superfamily. Cloning and overexpression demonstrated that both ... E.coliMGST cluster and SynMGST cluster). A major subgrouping is visible with MGST1,PGES and Insect in the upper part of the tree and the remainingfamilies ⁄ groups in the lower part. In the lower part, ... from the other puriﬁcation steps. The band was cut out from the gel followed by elution of the protein and the N-terminalamino acid sequence was determined using an Applied Bio-systems 477A instrument...

Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... for the synthesis of leukotrienes and lipoxins belonging to the eicosanoidfamily which are involved in many physiological processes[1]. In plants the biosynthesis of phytooxylipins is initi-ated ... providing the substrates for the activity of all the enzymes located downstream on the pathway, have a primary role in the biosynthesis of phytooxylipins and can affect their availability inside the plant ... sequences of the hazelnut LOX gene. The inverted repeats are in bold and their orientations are indicated by arrows. TATA and CAAT boxes and the initiatingATG are also in bold.4368 A. Santino et...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... [46]. The similarity in the Kmvalues is attributable to the uracil moiety binding in the same fashion [38], which could trigger the initialbinding of both substrates. In UpG, the binding of the guanine ... studies of the structure and binding sites of enzymes. 8. Inhibitor binding toribonucleases. J Mol Biol 45, 491–511.33 Ui N (1971) Isoelectric points and conformation of proteins. I. Effect of urea ... contrast, the exocyclicN6-amino group of adenine forms a hydrogen bondwith the side chain of Asn71, increasing the afﬁnity of RNase A for UpA. This hydrogen bond is apparent in the complexes...

Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc

... only the amino acid residues involved in the binding of the purine base were identiﬁed, while the ribose moiety was completely disordered [11].Mutation of two residues involved in the binding of adenine ... sites 1 and 2 and involved in bind-ing of adenylic nucleotides and BPG.Our results partially conﬁrm the molecular model-ling, suggesting that effector site 1 is the binding sitefor Ap4A and ... to phosphorylation of inosine,guanosine and a number of their analogues [2]. There-fore, in addition to being involved in regulation of purine intracellular pool, the enzyme is also responsibleKeywordscN-II...

Tài liệu Báo cáo khoa học: Post-translational modiﬁcation of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx

... Yersinia surface proteininvasin binds to b1 integrins on the apical surface of M cells, which facilitates translocation across the epithelium [4,5]. The pathogenicity and virulence of Yersiniae ... threonine kinasethat phosphorylates actin [12], binds the deubiquitinat-ing enzyme otubain 1 (OTUB1) [13,14], the smallG protein subunit Gaq [15] and interacts with mem-bers of the Rho family of ... In addition to actin, other host cell proteins have beenshown to bind to the virulence factor YpkA, includingOTUB1 [13]. In order to investigate the role of OTUB1 in Yersinia invasion of HEK293T cells,...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... analysis of substrate on the active site of HYDJs The substrate binding pocket in the active site was inves-tigated based on the homology model to obtain moreinformation on substrate binding in ... (B) The SGLs of HYDBpare shown as magentalines and those of HYDJsare shown in green. The transition state of D-p-HPH is shown in turquoise, and the four histidines coordinat-ing the metal ... substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were analyzedby substrate docking in the active site using AutoDock4[45]. Enzyme structures as the receptor...

Tài liệu Báo cáo khoa học: Post-translational modiﬁcations of the linker histone variants and their association with cell mechanisms docx

... agree-ment about the overall parameters of the fibre formedby folding the zig-zagging chain of nucleosomes in inactive chromatin, the location of the linker histone in relation to the nucleosome ... ubiquitinated in H1.2,H1.3 and H1.4 in human HeLa cells, but not in MCF7 cells. In mouse tissue, Lys116 of H1.1 and Lys46 of H1.2 and H1.3 in the spleen were the onlysites of ubiquitination. The ... number of these modifications is low, the density of modifications is much the same as in the rest of the lin-ker histones. It is the shortness of the N-terminal tailsTable 1. Alignment of chicken,...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... extents in the FNR–FMN subdo-main interface, including the interface in NOS (Fig. 8).Point mutations that neutralize charge pairing or intro-duce charge-repelling interactions may increase the Fig. ... rate [111–114]. The FMN–FNR sub-domain hinge (H1 in Fig. 5) is one of the least con-served motifs and is shorter in eNOS than in nNOS.Swapping the H1 hinge of nNOS into eNOS increasedits heme ... Role of the C-terminaltyrosine of ferredoxin–nicotinamide adenine dinucleo-tide phosphate reductase in the electron transferprocesses with its protein partners ferredoxin and ﬂavodoxin. Biochemistry...

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