... runs along the < /b> extended backbone of< /b> residues 65–64 and < /b> is partly exposed to the < /b> solvent(Fig. 2B) . On < /b> the < /b> other hand, the < /b> P3 moiety of < /b> E-64 ineach of < /b> the < /b> of < /b> the < /b> two ervatamin-C molecules in the< /b> asymmetric ... Evolution 100; Thermo Electron Cor-poration, Rockville, MD, USA). The < /b> range of < /b> substrateconcentrations and < /b> the < /b> time of < /b> incubation were standardizedfor each substrate. An extinction coefficient of < /b> ... have been described. On < /b> the < /b> other hand, asmentioned above, the < /b> 3D structure of < /b> ervatamin-Chas been published previously [27], and < /b> thereforeonly its binding interactions with E-64 will be dis-cussed...