Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... 2010 FEBS 4149MINIREVIEW Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression Hiroshi Sakurai and Yasuaki EnokiDepartment of Clinical Laboratory Science, ... in determining the target genes of each HSF. This minireview focuseson the molecular mechanisms of DNA recognition, chromatin modulation and gene expression by yeast and mammalian HSFs.Abbreviations3P, ... into the nucleus upon heat shock and may activate nonclassical heat shock genes, butdoes not activate classical heat shock genes (i.e. HSPgenes).HSF1 prefers long arrays of the nGAAn unit, whileHSF2...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... against the development of RA [61].The gene copy number variations of CCL3-like 1(CCL3L1), a nonallelic isoform of CCL3 encoded bydifferent genes and a potent ligand for CCR1 and CCR5, influenced ... joints of the hands and feet. Theinflammatory process is characterized by infiltration of inflammatory cellsinto the joints, leading to proliferation of synoviocytes and destruction of cartilage and ... inflammation, cell proliferation and angiogenesis. To date, several animal studies and human studies have shown the biological efficacy of specific antagonism of ligands and receptors in RA[45,63]....

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of environmental factors pdf

... 0.5–1% of the popu-lation and causes progressive joint destruction thatleads to the restriction of activities of daily living and deterioration of quality of life. Although the patho-genesis of ... PCRampliﬁcation of both NS1 and VP genes of B19 DNA in synovial membranes has revealed that the levels of B19 DNA are similar in RA and control synovialmembranes [11]. Thus, the contribution of B19 infec-tion ... 0.5–1% of the population. RA causes progressive joint destructionthat leads to the restriction of activities of daily living and deterioration of quality of life. Although the pathogenesis of RA...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of transcription factors ppt

... array of events underthe control of NF-jB, including cytokine and cyclo-oxygenase-2 expression, osteoclast differentiation and apoptosis, and the impact of these events on the path-ogenesis of ... RA. Expression of MMPsis proposed to be regulated by AP-1, and the upregula-tion of MMPs in the RA synovium correlates withincreased DNA- binding activity of AP-1 and increased expression of ... essential element in gene expression. The central role of transcription inthe process of gene expression makes it an attractivecontrol process for regulating the expression of genesin particular...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... PUF-8,Drosophila and human Pumilio and yeast Puf3 recog-ABCDFig. 6. Analysis of binding afﬁnity between APUM-2 and the FAS-2 3¢ UTR transcripts with nucleotide substitutions upstream and down-stream of ... that of other well-characterized members of PUF family. These proteinsmust bind to transcripts in the plant and regulate theirtranslation and ⁄ or turnover.A large number of APUM target candidates: ... been suggested in yeast and Dro-sophila, APUM proteins may be involved in the regu-lation of many aspects of growth and development.Interaction between APUM proteins and transcripts possessing...

Tài liệu Báo cáo khoa học: The sequentiallity of nucleosomes in the 30 nm chromatin ﬁbre pptx

... the DNA to mononucleosome size of samples con-sisting of a mixture of hepta-, octa- and nonanucleosomes and half the amount of hexa- and decanucleosomes (6- to 10-mer sample). (A) (a)Nonamer of ... for the ﬁbre stability of chicken erythrocyte chromatin [5,26]. The loss of linker histone causesexposure of the linker DNA to DNase I [27] and leadsto shortening of the chromatin repeat length ... mononucleosomes and the band S. DNA gel electrophoresis of the fractionsfrom Fig. 3D,E showed that approximately 90% of the DNA in the main peak as well as the mononucleo-somes and band S are all...

Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt

... S, Brandts JF & Lin LN (1989)Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Anal Biochem179, 131–137.H. Fernando et al. Chemokine ligand–receptor ... form and structured in thebound form. Knowledge of the dynamic characteris-tics of both IL-8 and N-domain before and afterbinding and whether binding is accompanied byrelease or retention of ... desolvation, loss of conformational ﬂexibility and dynamics) contribu-tions to binding, and such knowledge is essential forunderstanding the relationship between structure and the thermodynamics of binding.IL-8...

Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx

... half-life of % 13 and 33 min,respectively.Substrate specificity analysisAn analysis of the substrate specificity for DNA of theenzymes shows that they both cleave plasmid DNA, dsDNA and ssDNA as ... is plotted and the mean values are drawn.ABFig. 8. Cleavage of plasmid, dsDNA and ssDNA. (A) 14 nM VcEndAincubated at 23 °C for 5 min with plasmid (lane 2), dsDNA (lane 4) and ssDNA (lane 6). ... of VsEndA (Fig. 1). Thetheoretical pI was 9.61 for VsEndA and 8.62 forVcEndA. Expression and purificationFrom 7 L Escherichia coli culture, a total of 24 and 50 mg pure recombinant VsEndA and...

Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

... 35 (13%) carryan idi-1 gene, 72(27%)carryanidi-2 gene, 2 carry both anidi-1 and idi-2 gene, and 154 (59%) appear to be devoid of IPP isomerases.Phylogenetic analyses of 102 type II isomerases ... Germany).Cloning and expression of theidi-2 gene fromB. subtilisA DNA segment extending from bp position 184 997–186 043 of the B. subtilis chromosome was ampliﬁed byPCR using chromosomal B. subtilis DNA ... genomes of fungi, animals and plants. The analysis of theoccurrence of idi-1 and idi-2 genes in conjunction with themevalonate and nonmevalonate pathway in 283 completed and unﬁnished prokaryotic...

Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx

... Lankelma1,21Department of Molecular Cell Physiology, Institute of Molecular Cell Biology, Faculty of Earth and Life Sciences, Vrije Universiteit,Amsterdam, the Netherlands;2Department of Medical Oncology, ... expression of genes involved in cell cycle progression include Elk1, c-fos,c-Jun and c-myc [9,15]. The duration of ERK activation(transient or sustained) determines the repertoire of targetgenes ... targetgenes expressed [16], and also affects the type of cellularresponse that is evoked [17,18]. Activation of ERK isrequired for proliferation of ﬁbroblasts [19] and constitutiveERK activation...

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