•uence of zn and cu on cd nephrotoxicity
Heavy Metals in the Environment - Chapter 10 pps
... of the coaccumulation of Al and iron in both neurofibrillary tangles and in nuclei of tanglebearing neurons Also, concentrations of Al and iron in nuclei of tangle-free neurons and neuropil are ... high concentrations of Al, iron, and manganese in soil, drinking water, and vegetables (217) High concentrations of Al and iron have been detected in brain and spinal cord tissue of ALS- and ALS/parkinsonism-dementia-affected ... history of dementia, exploration of gene-environment interactions, level of education, and premorbid levels of cognitive functions are often not controlled For example, if geographical regions with...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
... After 24 h of incubation, 100 lL of fresh medium was added The cellular growth was evaluated 72 h after addition of oligonucleotides by assessing the incorporation of 0.5 mgÆmL)1 of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium ... Abbott, C.M (1998) The lethal mutation of the mouse wasted [wst] is a deletion that abolishes expression of a tissue-specific isoform of translation elongation factor 1alpha, encoded by the Eef1a2 ... from incubation of the nuclear protein extract with the same amounts of a total rabbit preimmune serum, which displayed only nonspecific competition (Fig 4, lanes and 7) The slight reduction in...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... the presence and app absence of ATP K m is the apparent dissociation constant for full length GST-IjBa substrate at saturation concentration of 60 lM ATP, and the dissociation constant for ATP ... inclusion of 10 mm MnCl2 in the kinase condition In vitro analysis of NF-jB signaling pathway Tris HCl, pH 7.5, and 10 mm MgCl2 and typically included: 50 ng of rhIKK2; varying concentrations of ... measure of the IL-1a concentration required to produce 50% of maximal response To investigate if different xatives and types of the culture substrate have an effect on the intensity of nuclear...
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Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf
... overexpression (n = 3) pathophysiological context since pathogenic mutations result in the loss of CARP binding to talin and FHL2 and, consequently, in the perturbation of its function [55] Regulation of ... localization by increasing the strength of its interaction with the sarcomere In addition, we investigated the modification of transcription factor activities induced by CARP over-expression and demonstrated ... 65 and 88 and the cleavage site is localized before amino acid 71, the region of cleavage would be between amino acids 65 and 71 Considering the location of the N-terminal extremity of DNter2 on...
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Elucidating the role of dok 3 in b cell receptor signaling using gene knockout mice 2
... high levels of surface IgM, low levels of B220 and IgD and moderate levels of CD5 , and absence of CD2 3 In contrast, conventional, or B-2, cells are the predominant B cells found in secondary lymphoid ... such as spleen and lymph node, and in the circulation B-2 cells express high levels of B220, IgD and CD2 3 and moderate levels of IgM and lack surface CD5 expression The B-1 population can be further ... various questions and to understand the role of Dok-3 in B cell development and activation, we have generated the Dok-3 deficient mice and focus the current study on the effect of Dok-3 on B cell...
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Elucidating the role of dok 3 in b cell receptor signaling using gene knockout mice
... embryonic fibroblasts Culturing of ES cells Preparation of DNA for transfection Transfection and selection of ES cells Picking and freezing down of ES clones in 48well plate Expanding ES clones ... 2.7.1 Buffers and solutions 2.7.2 Immunoprecipitation, western blot and antibodies 2.7.3 Isolation of membrane fraction 2.7.4 Nuclear fractionation and gel shift assay 2.7 Molecular and cellular ... Mammalian cell culture methodology 60 61 61 62 62 62 Cell culture media Purification of splenic B cells Purification of bone marrow cells Purification of thymocytes Purification of lymph node...
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Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf
... and S1 pocket maturation but at the same time an unaltered conformational distribution of the N-terminal tail and a normal response to TF Discussion Fig N-terminal pegylation of G372A-FVIIa and ... most active conformation of FVIIa but not in order to respond to TF Results Determination of active concentrations Titrations of G372A-FVIIa and FVIIa with d-PhePhe-Arg-chloromethyl ketone (fFR-cmk) ... backbone amide of Gly372(223) participates in a hydrogen bond with the backbone carbonyl of Ser333(185), thus connecting activation loops and in the active conformation (Fig 5A) [26] In addition,...
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Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
... l-glutamate became more pronounced upon the addition of Tris The location and orientation of l-glutamate were determined on the basis of the lower electron density of FEBS Journal 277 (2010) ... residues of the T and TG (T ⁄ TG), N and G (N ⁄ G), G and TG (G ⁄ TG), and N and T (N ⁄ T) structures are superimposed Effects of (A) L-glutamate (T ⁄ TG and N ⁄ G) and (B) Tris (G ⁄ TG and N ⁄ T) on ... sites of Mglu and Bacillus glutaminase in the presence and absence of ligands Backbone atoms of the seven conserved amino acid residues and the three conserved tyrosine residues in Mglu and Bacillus...
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Báo cáo khoa học: Identification of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot
... staurosporine and 100 lm of phosphotyrosine phosphatase inhibitor BiPy (in addition to sodium vanadate and sodium molybdate) to both control cells and BiPy-treated cells at the beginning of homogenization ... applied facilitates the identification of phosphopeptides in general As most regulatory phosphorylation events occur at serine and threonine residues and persist longer in the cells than the highly ... phosphorylation necessary for the final comparison of tyrosine-phosphorylated proteins Table Phosphorylation sites of mouse and of human emerin Phosphorylation site Number of phosphorylated residues Peptide...
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Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx
... infection induced spermine and spermidine expression in fibroblasts [41,44] Another study reported induction of ArgI and ArgII mRNA in the cornea during HSV infection, but protein concentrations and ... enzymatic activity in the context of virus infection in an IRF-3dependent and IFN-independent manner Moreover, expression of a constitutively active form of IRF-3 leads to induction of spermine, which ... (1983) Contribution of macrophage arginase in the intrinsic restriction of herpes simplex virus replication in permissive macrophage cultures induced by gamma-interferon containing products of activated...
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Báo cáo khoa học: 15-Deoxy D12,14-prostaglandin J2 suppresses transcription by promoter 3 of the human thromboxane A2 receptor gene through peroxisome proliferator-activated receptor c in human erythroleukemia cells ppt
... expression and function, HEL cells were preincubated with 10 lm 15d-PGJ2 for 24 and 48 h and its affect on TP-radioligand binding and on 4757 Effect of 15d-PGJ2 action on TP gene expression A A ... )159 and )148, respectively, are required for binding Discussion TXA2 is a potent mediator of platelet activation and aggregation and a constrictor of vascular and bronchial smooth muscle and ... synthesis and TXA2 4755 Effect of 15d-PGJ2 action on TP gene expression action [42–45], these data point to an added benefit to the current use of PPARc agonists in the treatment of cardiovascular...
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Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf
... compared the effects of the maleimide on the phosphorylation of PKB and GSK3 and upon the stimulation of glucose transport in response to a submaximal and maximal insulin concentration Fig shows that ... suggesting that activation of PI3K precedes that of GS This proposition is consistent with the observation that the inhibition of GSK3 (mediated by PKB) and the activation of PP1 in response to insulin ... software and considered statistically significant at P values < 0.05 Fig Effects of insulin and wortmannin on GSK3 a and b activity in L6 myotubes and relative abundance of GSK3 isoforms in L6 and...
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Báo cáo " DENOTING THE NUCLEAR ISOTOPES IN EXPERIMENTAL GAMMA SPECTRUM" pptx
... structure containing the infomations of an energy level The exhibit of the information of the spectrum is multiform It depends on each experiment and on the taste of user In our case, it is contained ... if(fabs(Dongvi.Nlgdv[i]-Trg->Nluong) Sigmnlg) Snlgdvctrph+=1; Tam-4Nlgdv[i]=Trg->Nluong; Tam->Sigmnlg[i]=Trg->Sigmnlg; Tam->Cuongdo[i]=Trg->Cuongdo; Tam->Sigmcdo[i]=Trg->Sigmcdo; break; ... Nguyen Trung Tinh contradiction between the number of energy levels needed for identification of a nuclear isotope and the ability denoting them of experiment The more energy levels of a nuclear isotope,...
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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx
... % of control 3h SAE1 600 % of control ** ** % of control 10 000 % of control Cyp11a1 30 000 % of control % of control A F.-M Yang et al h 12 h 24 h Fig The cAMP pathway regulates the expression ... SUMO-1 conjugation in rat granulosa cells Mutation of three or more sites abolished the accumulation of LRH-1 in nuclear bodies It is well known that the control of ovarian function and steroid ... the effect of cAMP signaling on SUMO pathway is complex and cell type dependent Notwithstanding, the up regulation of E1, the down regulation of E2 or E3 and the increased expression of SENP2 lead...
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FUTURE OF THE NUCLEAR SECURITY ENVIRONMENT IN 2015 pptx
... fuel cycle based on a harmonization of the current U.S., Russian, and IAEA proposals This common vision does not enhance a sense of discrimination among the non-nuclear weapons states because ... NON-PROLIFERATION OF NUCLEAR WEAPONS The Treaty on Non-proliferation of Nuclear Weapons (NPT) entered into force in 1970 and continues to be a positive influence in the context of resolving issues of nuclear ... charter of the National Academy of Sciences, as a parallel organization of outstanding engineers It is autonomous in its administration and in the selection of its members, sharing with the National...
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Báo cáo khoa học: The in vitro nuclear aggregates of polyamines pot
... separation of polyamine solutions prepared by changing the concentration of a single polyamine and keeping the concentrations of the other two constant (24 mM) In the case of variation of Spm concentration, ... which depends not only on the different concentrations of the solutes but also on the electrostatic interactions in the solution Table Relative concentrations and recoveries of polyamines in ivNAPs ... additional conceptual elements for molecular modeling and determination of NAP functions In this article, we report findings indicating structural and functional analogies among extractive and synthetic...
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Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf
... mg of purified CT-peptide per liter of bacterial culture Effect of the CT-peptide on mPC1 ⁄ enzymatic activity The effect of various concentrations of the purified CT-peptide on the cleavage of ... addition to the derived Ki value, one must consider the values of two parameters taking into consideration the formation of a ternary complex factor which also contributes to the release of product, ... well as removal of the CT-peptide This transformation may be enhanced initially by the low concentration of CT-peptide to increase production of the active 66 kDa form Accumulation of products (decreasing...
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báo cáo hóa học:" yuDetecting the percent of peripheral blood mononuclear cells displaying p-STAT-3 in malignant glioma patients" pot
... relationship between PBMC pSTAT-3 and Treg fraction expression The lack of a straight trend of the Loess curves indicates that there was not a correlation between PBMC expression of p-STAT-3 and ... Loess smooth curves were presented to demonstrate the relationship Comparison of Treg fraction difference between normal and tumor patients was conducted using Wilcoxon tests All computations were ... relationship between tumor pSTAT-3 and PBMC p-STAT-3 expression The lack of a straight trend of the Loess curves indicates that there was not a correlation between tumor and PBMC expression of pSTAT-3...
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Báo cáo sinh học: " Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra" ppt
... 2Fclone17 1Hclone26 100 1Hclone13 1Fclone18 3Fclone9 1Fclone26 2Hclone28 3Fclone3 1Fclone12 GenotypeII 2Hclone27 2Hclone29 3Hclone16 1Hclone25 3Hclone15 1Fclone7 2Fclone29 2Hclone24 1Fclone6 ... 3Hclone30 3Fclone13 3Hclone12 2Fclone15 2Fclone14 3Hclone1 3Fclone15 1Hclone29 2Fclone30 100 3Fclone26 Substitution/site Figure DEN-3 clinical isolates and 24 evolutionary relationships of the ... sequencing and identification of heterogeneous regions from trace file Clonal sequencing and characterization of the mutation spectra of the regions Figure in RNA of DENV-3 Strategy1 clonal-sequencing...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25