... between the hypophosphorylated initiation isoform IIA and the hyperphosphorylated elongating isoform IIO thatexhibits reduced electrophoretic mobility compared with the IIA isoform. Whereas the ... DNA replicationPol e is one ofthe three major replicases in the eukary-otic cell [22]. Therefore, we tested the effect of the replicative state ofthe cell on the interaction betweenPol e ... Following phosphatasetreatment, the cellular fraction of RNA pol II thatassociated with Pol e showed increased mobility, cor-responding to that of isoform IIA. The isoform IIOwas also lost from...
... discussion, they share their excitement with the author. They conclude that the activities so very interesting that they can learn with ease. The activities also bring them many chances to practice their ... discuss what they have seen and heard at the end ofthe presentations.2. The students present their objects within the two minutes limit.3. When all the students have finished, writethe following ... collected from other sources- the survey questionnaire. The author ofthe study observed the C.QDL 05.2. The observation is carried out with a set of checklist made by the author including the students’...
... The intracellular concentration of hemeis maintained by the rate of its synthesis and degrada-tion [1]. Many enzymes and their regulators areresponsible for heme synthesis [1,2]. On the otherhand, ... normalized with respect to the intensity ofthe 18S rRNA signal. The ratio of each normalized value to the control value in untreated cells at 6 h is shown as the relativeexpression level of HO-1 ... analysis of HO-1 andHO-2 proteins. The intensity representing HO-1 or HO-2 protein was normalized with respect to the intensity ofthe b-actin signal. The ratio of each normalized value to the control...
... m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminalGlcNAc. The position ofthe second Fuc 3-linked to the internal GlcNAc forming an internal Lexdeterminant ... foranalysis ofthe branching patterns and blood groupdeterminants with low (picomole) amounts ofthe underi-vatized oligosaccharides. The significant aspects of thisstrategy are the combined use of ... downfield from the Fuc IX H5 proton. ForTFiLNO, the same temperature would have resulted in totaloverlap ofthe H2O signal withthe Fuc X and XI H5protons. Therefore the temperature of 27 °C was...
... live in the northeast of Ethiopia, confined to the most arid part ofthe country, where there is least access to educational, health and other social services. Tuberculosis (TB) is one ofthe major ... (16.4%). The discussants also suggested these same factors as the cause of PTB. All the discussants and the majority (74.3%) ofthe interviewees reported that persistent cough as the main symptom of ... region. Lack of knowledge about TB could affect the health-seeking behaviour of patients and sustain the transmission ofthe disease within the community. In this study, we assessed the knowledge...
... compatible with the AVIC-ZH77. *5: Free of charge for up to three years from the month when use ofthe data services starts (from the day the user starts using the services until the end of that ... distance indicator The lock-on range for the target that indicates the distance between the driver’s vehicle and the vehicle in front has been extended. The size and color ofthe inter-vehicular ... all of the roughly 700,000 km of roads in Japan*10 Route searches are based on the Smart Loop traffic congestion informationđ system, which targets all of the roughly 700,000 km of roads...
... characteristic of PNAặDNA complex formation has been the highdegree of discrimination for sequence selectivity with the complementary strand of DNA [11,13] given the significantly less stable nature ofthe ... is because ofthe provenefficacy ofthe designed PNA to down-regulate the gene expression ofthe mutated allelle, while sustaining the transcription ofthe wild-type allele [29,29a]. The present ... amide(N1Â) hydrogen pointing inside the helix. On the otherhand, this facilitates in the formation of hydrogenbond with N3 of purines and O2 of pyrimidines eitherdirectly (Fig. 10A,C,E,G) or...
... in the analysis of game-theoretic models (chapter 10) –how one playerresponds to the actions of another on the assumption of a speci…c formof the rules ofthe game.We use the comparative statics ... extra cost of producing 4q.” In other words, in the neighbourhood of the optimum, the appropriate “value ofthe constraint in (2.12) the Lagrangemultiplier –is the marginal cost of output at ... the method of proofis not particularly illuminating or is rather technical. Throughout each chapter there are footnotes that focus on detailed points of the argument. These take theform of...
... also interact with each other under the ex vivo conditions of Y2Hsystem. However, the AH109 strain transformed with either the pGAD T7 MP clone or the pGBK T7 CPclone alone did not form colonies, ... systemically within the hostplant [11]. The present study comprised biochemicalcharacterization ofthe MP encoded by ORF1 of SeMV, a member ofthe genus Sobemovirus. Analysis of the deduced ... mutants of MP were performed to determine the domain responsible for the interaction of MP with CPusing ELISA and yeast two-hybrid (Y2H) assays.Deletion ofthe N-terminal 49 amino acids of the SeMV...
... were observed in the presence of ATP.No change in the mobility ofthe Ure2p–Ssa1p com-plexes was detected in the presence of ATP. The stoichiometry of Ure2p and Ssa1p within the 120and 160 ... built from the X-ray structure ofthe C-terminal domain of Ure2p and integrates the finding that the N-terminal domain of Ure2p is flexible. An enlargement ofthe region of Ure2p involved in the interaction ... difference (p.p.m.) withthe theoretical monoisotopic mass ofthe identified peptide are given. The presence ofthe modified peptides in the Ure2p, Ssa1p and Ure2p–Ssa1p complexes with apparent molecular...
... completed, the depositor shall bring the errors or omissions therein, if any, to the notice ofthe Post Office Savings Bank forthwith and in the event ofthe depositor’s failure to do so, the Post Office ... person the right to receive payment ofthe sum for the time being due on the savings certificate on the death of holder thereof and before the maturity ofthe certificate, or before the certificate ... (iii) the period from the date of opening the account to the date of death ofthe depositor or surviving depositor, as the case may be, is not less than two years. (iv) The age ofthe depositor...
... degradation ofthe protein. The latter half ofthe kinase inhibitory region and the entire extended SH2subdomain form a single a-helix. The mapping ofthe true SH2 domain,and the location of its ... atotal of 290 lL of 80–200 lm ofthe gp130 peptide. The heat of dilution ofthe gp130 peptide into buffer was deter-mined in control experiments and subtracted from the rawdata ofthe binding ... exam-ined the sequence ofthe 19 structured residues immedi-ately downstream ofthe PEST region and found a highlikelihood that they constitute the BG loop and bGstrand ofthe SH2 domain of SOCS3...
... interested in the size ofthe variation relative to the size ofthe observation. Thus, we can compare the variability of a test price with a mean of 800 Euros to one of 80,000 Euros. Thestandard ... 7-times the median ofthe average price and the outlier lab 100-times the median ofthe average price. One possible reason for the majority of prices around 1,800 Euro might have to do withthe ... Italy, the Netherlands, Switzerland and the UK. In the next section ofthe article we briefly discuss a few methodological issues and describe the design ofthe study. The questionnaire and the...
... the carb-oxyltransferase and the biotin domain. The dynamics of conformational motions within the catalytic cycle of the enzyme probably also includes the motion of the entire soluble part of ... residue ofthe histi-dine triplet near the C terminus of c is specificallyrequired for complex formation withthe a-subunit. The c-subunit therefore plays an important role in the in the assembly of ... a crucial role in the formation of the a ⁄ c-complex. H78 is part of a cluster of fourhistidine residues near the C terminus ofthe c-subunit of which H77 and H82 together with D62 were shownto...
... measure the number of coloniesformed. The bactericidal activity was expressed as killing(%), using the formula [27]:Killing %ịẳẵ1 CFU in the sampleị=CFU in the control100ể FEBS 2003 Three isoforms ... measured withthe DC-protein assay kit using BSA as standard. The concentration of purified and syntheticchrysophsin-1 and chrysophsin-2 was obtained from the A280[24], and that of chrysophsin-3 with ... withthe averagemolecular mass of His (Table 1). On replacing the unknownamino acids of fragment 3 with His, the theoretical averagemolecular mass (1365.62 Da) of fragment 3 calculated fromthe...