... carnitine: 1 mm (for standard comparison withother published data), and a concentration equal tothe Km for carnitine of each enzyme (for comparisonbetween mutants). In this article, we show ... sequence as being responsiblefor the pecu-liar kinetic characteristics of this enzyme, acting as anegative determinant for malonyl-CoA sensitivity.Asp17 may account, at least in part, for the highdegree ... nat-urally occurring variation, Glu17, isresponsiblefor both the low malonyl-CoA sensitivity and high carnitine affinity characteristics of the pig enzyme.This is the first demonstration that a single...
... active conformation, Val371 forms contactswith neighboring residues that are important for stabi-lizing the active state (e.g. Asp189, whichis part of theS1 pocket responsiblefor the binding ... substitution of Val371 toisoleucine might prevent the full development of theactive conformation. This hypothesis is further con-firmed by the results of FIX proteolytic assays, which showed a slight ... sequence identity of 30–45%), thisposition is occupied solely by a valine. This conservedamino acid is replaced by isoleucine in the Val371IleFXI mutant. Interestingly, an isoleucine residue natu-rally...
... factor that favors lysogeny and is responsiblefor primary control of the lambda develop-mental decision for lysis or lysogeny [4,7–9]. In theabsence of CIII, CII is rapidly degraded by the ATP-dependent ... However, this proteolysis is muchslower in vitro, because of a lack of chaperone machin-ery. The in vitro proteolysis requires ATP [14]. Weexamined the proteolysis of purified r32-C-his (1.2 ... onproteolysis of the heat shock sigma factor r32, anothersubstrate of HflB. Like proteolysis of CII, r32proteol-ysis requires ATP. Both CIII and CIIIC inhibit thisproteolysis, with CIIIC...
... prior to hydrolysis. The low-affinity state wasobserved to be retained immediately post-hydrolysis. Therefore, the dissoci-ation of phosphate and ⁄ or ADP is likely to be responsiblefor resettingof ... high-affinityconformation for the transporter, whichis supportedby the restoration of high-affinity binding in the ADP-bound conformation. That this step of the catalyticcycle is associated ... of ATPper se is the ‘power stroke’ for drug translocation, andthat energy obtained from the hydrolysis process is used to reset the transporter. That ATP binding is responsiblefor the shift...
... hydrolysis), corresponding to that expected for theF variant. This result is the first evidence for the heterozy-gous status (M/F) of the individual goat milk analysed.In addition to this, t ... first one is an ACRS-PCR technique [26], the principleof whichis to create a TaqI restriction site (TCGA) by usinga mismatching primer (C9LM*) which allows both the Fand M alleles to be discriminated ... subsequently distin-guished after a second amplification which allows discrim-ination between the alleles on the basis of the f ragment sizes(Fig. 8 , Step IIA).In the first step, a 266-bp (265 bp for...
... 4. A. ought B. bough C. thought D. brought5. A. spoor B. door C. floor D. force6. A. poisonous B. noise C. tortoise D. buoy7. A. golf B. adulthood C. wood D. bosom8. A. wool B. mood C. took ... scheme C. skeptical D. schematic7. A. bronchitis B. chestnut C. chronometer D. chrisma8. A. chauvinism B. exchange C. teacher D. childrearing9. A. bristle B. indict C. Connecticut D. Vinci10. ... D. foreign10. A. gnome B. assignment C. designer D. magnificent SOUNDS 151. A. locate B. geologist C. horizon D. gallop2. A. rough B. tough C. through D. much3. A. environment B. information...
... scheme C. skeptical D. schematic7. A. bronchitis B. chestnut C. chronometer D. chrisma8. A. chauvinism B. exchange C. teacher D. childrearing9. A. bristle B. indict C. Connecticut D. Vinci10. ... 4. A. ought B. bough C. thought D. brought5. A. spoor B. door C. floor D. force6. A. poisonous B. noise C. tortoise D. buoy7. A. golf B. adulthood C. wood D. bosom8. A. wool B. mood C. took ... D. foreign10. A. gnome B. assignment C. designer D. magnificent SOUNDS 151. A. locate B. geologist C. horizon D. gallop2. A. rough B. tough C. through D. much3. A. environment B. information...
... that eitherone isresponsiblefor everything or one has refused responsibility.This is how Levinas answers those who say that to be responsible for everything is to be responsiblefor nothing.In ... on this account, butthat does not mean that the relationto being in which the ethical is situated is not crucial. This is whyit is not irrelevant what is said in the saying, just as it is not ... toward the concrete, which is where the encounter takes place. The point is not just toshow that such acts are impossible for an existence that is concernedonly for its own existence, as they would...