treatment of bladder cancer cell lines with live bcg and lyo bcg for ros and cytokine comparisons
Mycobacterium bovis, BCG modulation of gene expression in bladder cancer
... occur with lyophilized BCG Before lyo BCG and live BCG treatment in vitro with human bladder cancer cell lines were compared, the integrin α5 and β1 expression and BCG internalizing capacity of ... Figure 4.2 Profile of live BCG and lyo BCG internalization of MGH cells 89 Figure 4.3 Histogram representing the ROS changes induced by treatment of MGH cell line with lyo BCG or live BCG 92 Figure ... Treatment of bladder cancer cell lines with live BCG and Lyo BCG for ROS and cytokine comparisons …………………………………………………………… 42 2.2.3.4 Cycloheximide treatment, BCG internalization and cytotoxicity...
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PURIFICATION OF ORGANIC CHEMICALS pot
... 101111 AcOH and diluted with 50ml of H20, (B) 5g KI in lOOml of H20 and (C) 0.3g ninhydrin in lOml of AcOH and 90ml of H20 The spray is prepared by mixing (A) and (B) with 1.5ml of (C) and stored ... refluxed for 2h with 15ml of MeOH and 2ml of 98% H2SO4 (cool when mixing this soh) Pour into 10 volumes of H20 and extract with the minimum volume of CHC13 to give clear separation of phases ... trimethylacetic acid and C9 and C13 acids Dissolve 15g of acid in CCl4 (3OOml) and shake with lOml of 15N aqueous NH3 and the ammonium salt separates and is collected Acid impurities form soluble ammonium...
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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc
... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and ... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the resolution increases with the volume Vs of the stationary phase, with the selectivity (K2/K1) or the difference between K2 and K1, and the reduction in band width Within the eight tested solvents...
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PURIFICATION OF LABORATORY CHEMICALS pot
... Salts and organic esters of some inorganic acids Sol vents 5 5 6 6 METHODS OF PURIFICATION SOLVENT EXTRACTION OF REAGENTS AND SOLVENTS AND DISTRIBUTION DISTILLATION Techniques Distillation of liquid ... adsorbents and solvents Preparation and standardisation of alumina Preparation of other adsorbents Silica Diatomaceous earth Charcoal Cellulose Supelco products Flash Chromatography (FC and HPFC) ... QUESTION OF PURITY SOURCES OF IMPURITIES PRACTICES TO AVOID IMPURITIES Cleaning practice Sily lation of glassware and plasticware 3 SAFETY PRECAUTIONS ASSOCIATED WITH THE PURIFICATION OF LABORATORY...
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purification of carbon nanotubes
... clear that the purity and the yield of SWCNTs with nitric acid treatment depend on the concentration of the nitric acid and the time of reflux The treatments of Fig – Mass balance of the normalized ... oxidants for gas phase oxidation include air [40–46], a mixture of Cl2, H2O, and HCl [47], a mixture of Ar, O2, and H2O [48–50], a mixture of O2, SF6 and C2H2F4 [51], H2S and O2 [52], and steam ... wall of CNTs XPS can give a quantitative characterization of type and content of functional groups Raman spectroscopy 100% pure SWCNTs should be characterized by one G band with RBM and without...
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Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot
... 20 mm Glu and 20 mm glyoxylate for GGT, 20 mm Glu and 30 mm pyruvate for GPT, 40 mm Ala and mm glyoxylate for AGT, and 10 mm phosphoserine and 10 mm 2-OG for PSOT For the AT1 assay, the pH in the ... description of the characterization of bacterial GGT and AGT at an enzymatic and gene level Comparison of the amino acid sequences with known enzymes showed the phylogenetic position of 1880 each ... added For GOT, GGT, GPT, AGT and PSOT assays, substrate concentrations were modified as follows: 100 mm Glu and 10 mm oxaloacetate or 1882 10 mm Asp and 10 mm 2-OG for GOT, 20 mm Glu and 20 mm glyoxylate...
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armarego - purification of laboratory chemicals 5e
... preferred because of the greater ease and efficiency of separating crystals and mother liquor, and also because of the saving of time and effort, particularly when very small crystals are formed or when ... they are of a standard quality Plates of a standardised silica gel 60 (as medium porosity silica gel with a mean porosity of 6mm) released by Merck have a specific surface of 500 m2/g and a specific ... disadvantage Sepharose (e.g Sepharose CL and Bio-Gel A) is a bead form of agarose gel which is useful for the fractionation of high molecular weight substances, for molecular weight determinations of large...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... Infections of JJHAN cells with HHV-6A (U1102) Infections of JJHAN cells with HHV-6A (U1102) A Quantifications of cellular (diamonds) and extracellular (squares) HHV-6A DNA The log10 of the viral ... infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained with DAPI (blue) to reveal cell nuclei Light microscopy pictures are magnified 20× and fluorescent ... bars) B and C Light microscopic analyses of HHV-6A- and mock-infected JJHAN cells at dpi Note the CPE, i.e enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... Infections of JJHAN cells with HHV-6A (U1102) Infections of JJHAN cells with HHV-6A (U1102) A Quantifications of cellular (diamonds) and extracellular (squares) HHV-6A DNA The log10 of the viral ... infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained with DAPI (blue) to reveal cell nuclei Light microscopy pictures are magnified 20× and fluorescent ... bars) B and C Light microscopic analyses of HHV-6A- and mock-infected JJHAN cells at dpi Note the CPE, i.e enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110...
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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc
... GP1pMAL-c2x:GP1 Figure gami cells transformed with construct from E coli Rosetta Expression and purification of LASV GP1 from E coli Rosetta gami cells transformed with construct pMAL-c2x:GP1 ... against linear and conformational epitopes Purified LASV NP cross-reacted significantly with most MHAFs of Old and New World origin, with the Table 2: Cross reactivity of Old and New World arenavirusspecific ... conditions: 98°C for one 30 sec cycle and then 35 repeated cycles of 98°C for 10 sec, 59°C for 15 sec, and 72°C for 30 sec, followed by a final extension at 72°C for Table outlines each of the nucleotide...
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Báo cáo hóa học: " Temperature sensitive influenza A virus genome replication results from low thermal stability of polymerase-cRNA complexes" doc
... the interaction of polymerase with cRNA was significantly less stable than with vRNA, a finding with implications for the regulation of viral RNA synthesis and for the adaptation of influenza viruses ... protein and RNA content by western blot and primer extension respectively For this, protein was concentrated by precipitation with methanol-chloroform [74] and RNA was isolated by phenol:chloroform ... for m- c and vRNA were quantified by densitometry The ratios of cRNA:mRNA and vRNA:mRNA were calculated and are shown as the fold change (average ± S.D.) in ratios between 31°C and 39°C for cells...
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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
... temperature for (+)-1, (-)-1, 13, 14 and 15 was 60°C for followed by a gradient of 10°C/min to 180°C maintained for Detection temperature for 16 was 120°C For 17, 240°C for followed by a gradient of 2°C/min ... rpm for another 16 h of cultivation Gene expression analysis Gene expression analysis was performed with crude cell extract Samples standardized to cell amount were taken during cultivation Cells ... was introduced using the sites of restriction endonucleases NdeI and XhoI for cloning with the BCA-kit and a standard curve of BSA in the same buffer in a range of 2-0.005 mg/mL was used Samples...
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purification of some heavy metals from paper making
... ACP-AES determination of plant samples of the content [J].Spectroscopy and spectral analysis,2005,25(4):563-566 [15] Romheld V The role of phytosiderophores in acquisition of i-ron and other micronutrients ... depth of 20 cm in each pool Then, reed roots are transplanted into each pool with a spacing of 0.04 m × 0.04 m The actual growing state of reed roots is shown in Figure Based on the standard of ... pool and divide them into root, stem and leaf to be tested after processing and decomposition[14] Four kinds of heavy metals (Cu, Zn, Pb and Cd) content of water and plants are tested by inductively...
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báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps
... end of each hour for SDS-PAGE analysis Cell lysis and protein extraction Bacterial cells were harvested by centrifugation of cell culture at 4000 rpm, 4°C for 10 Supernatant was aspirated off and ... considerable amount of non-specific proteins eluted in conjunction with this protein Final purity of the purified proteins is without any doubt an excellent measure of the performance of protein purification ... yield, and percent of protein recovery and loss were determined for each Nickel-magnetic matrix, each Page 10 of 11 bead concentration and each recombinant protein The sum of the specific band densities...
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Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx
... primary cell cultures of porcine origin The method is both labor intensive and costly, with the additional hazard of requiring the handling of large quantities of infectious virus [21] Economic and ... Expression and purification of polyhistidine-tagged VP2 of PPV We have previously shown that formation of chimeric VLPs of PPV fusion constructs is feasible in pET-PPV with E coli cells (Qi T ... concentration of about × 106 cells per ml of culture medium (30 ml growing volume) and infected with the recombinant viruses at a multiplicity of infection (MOI) of 2-3 At 72 h postinfection (p.i.), cells...
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
... a volume of 30 μl of PBS, as follows: PBS containing μg of CT adjuvant (Sigma), 15 μg of p24, 30 μg of RTB/p24, 15 μg of p24 mixed with μg of CT and, 30 μg of RTB/p24 mixed with μg of CT adjuvant ... between RTB/p24 and asialofetuin was performed We prepared a sepharose column for affinity chromatography with immobilized asialofetuin Five mg of asialofetuin were diluted in 0.1 M NaHCO3 and 0.5 M ... were coated with 200 ng/well of purified p24 in 50 μl of PBS and incubated at 4°C overnight Following three washes with 150 μl/well of PBST, wells were blocked with 200 μl/well of PBSM for h at...
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PURIFICATION OF SIMPL ANTIBODY AND IMMUNOFLUORESCENCE OF SIMPL SUB-CELLULAR LOCALIZATION IN RESPONSE TO TNFα- AND IL-1
... cytotoxic T cells as well as memory T cells The helper T cells remain in the lymph tissue where they secrete cytokines and chemokines to manage the activity of B cells, cytotoxic T cells, and macrophages ... Cellular based assays that utilize antibodies include immunofluorescence microscopy and fluorescence activated cell sorting (FACS) In immunofluorescence microscopy, cells are fixed to allow for ... family of transcription factors that greatly impacts the fate of the cell It can affect the fate of cells in many ways including induction of anti-apoptotic signaling, regulation of cell cycling, and...
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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene
... Khan and Yanlin Shi for being excellent research partners Finally, I could not thank enough to my dear mom and dad for their unconditional love and support and their always being there for me Without ... -20˚C 2.2.3 Detection of Chitinase Isoforms on Native Gels Acidic chitinase isoforms were separated and detected according to the method described by Trudel and Asselin (1989) with minor modifications ... chitinase caused a great loss of activity (Andersen et al., 1997; Iseli-Gamboni et al., 1998) In addition, mutation of Tyr 123 of a Zea mays chitinase and a similar tyrosine of an Arabidopsis chitinase...
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purification of phytase from aspergillus fumigatus
... years because of the interest in such enzymes for reducing the levels of inorganic as well as organic phosphate pollution in livestock areas, and also improving nutritional values of organic phosphate ... Preparation of fungi strain and culture medium Aspergillus fumigatus was maintained in PGA medium and incubated in 45°C for days Determine the centration of A fumigatus spore by hemocytometer and adjust ... Inoculation of 1mL spore (108/mL) of A fumigatus Incubated for days, 35°C Homogenization of fungi biomass in 50mL sodium acetate buffer 0.02M, pH 5.5 Centrifugation for 20 minutes, 13000 rpm, 4°C Take off...
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THE CONTRIBUTION OF CLAMS ON TIDAL FLAT PURIFICATION CAPACITY
... sediment with (A) and without (B) clams Index and indicated 0-2 cm and 9-11 cm depth of sediment, respectively be related with DO and substrate limitation in the deeper sediment High concentration of ... sediment without clams DOC removal (%) 100 80 60 40 20 without clam with clam 0 14 Day 21 28 Figure Biodegradability of organic matters in tidal flat sediment with and without clams Degradation of ... variation of NH4-N concentration in pore water of tidal flat sediments with and without clams Biodegradability and molecular weight distribution of organic matter Figure shows that more than 80% of...
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