the role of teachers schools and communities in quality education
Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf
... resultfrom the modifications in the interactions between the catalytic glutamates and the residues R97 and Y331,indicating that these residues play a role in the modulation of the Sfbgly50 pH optimum.Sequence ... mMBicine buffer 7.0–8.5). The pH stability of Sfbgly50 was checked by incubation in the same buffers for a time equal to the assay time and thendetermining the activity remaining at the optimum ... [10,12].Nevertheless, it is not possible to assume that the same isoccurring in the mutant R97M, because there is no data on the interactions between the substrate and the arginineresidue.Finally, another...
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Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
... by the amount of internalized agonist[20]. As the internalization is expressed in percentage of total binding, decreasing the binding affinity of the Role of helix 8 and C-termini in bradykinin ... 2004 FEBS The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2bradykinin receptorsAlexander Faussner1, Alexandra Bauer1, Irina Kalatskaya1, ... addition of scintillation fluid. Nonspecific bindingwas determined in the presence of 5 lm unlabeled agonist and subtracted from the total binding to calculate the spe-cific binding.Internalization of...
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Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf
... of GSK3 (using Li or SB-415286)appears to be sufficient for inducing activation of GS in muscle and fat cells, and that inhibition of the kinasepotentiates insulin action in muscle of insulin-resistant ... increases in glucose uptake and glycogen synthesisallowing for more effective ÔchannellingÕ of glucose intoglycogen in response to insulin. However, one of the potential difficulties in interpreting ... phosphatidylinositol3-kinase, p70, S6 kinase and glycogen synthase kinase-3 activity in L6 muscle cells: evidence for the involvement of the mammaliantarget of rapamycin (mTOR) pathway in the L-leucine-inducedup-regulation...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
... phenylalanine was thereforemutated to Ala, Leu, Trp or Tyr. These mutations may in uence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme ... PAA and derivatives thereof, while maintaining the hydrophobicity of the binding site.To test the effect of the mutations on the specificity forphenylacetylated substrates, the steady-state kinetic ... kcatrepresents the rate of acylation and Kmequals the binding constant of the substrate to the free enzyme. The results then indicateÓ FEBS 2002 Role of active-site phenylalanines in penicillin acylase...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
... unsure of the mechanism of DNA-induced stabilization of the protein, and whe-ther it is related to the normal RNA-binding function of coat protein, as RNA binding had no effect. The increased ... understood, and we have investigated thesequestions in this work. We sought to determine the role of protein–RNA and protein–protein interactions in virus stability, measuring the effects of urea,GdnHCl ... against all the forms of denatura-tion we tested. This is consistent with the observation of increased stability of the single-chain version of the wild-type dimer [30] and is reminiscent of the...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx
... visualized the mineralizingcells with 2% Alizarin Red. After rinsing in distilledwater and air drying the samples, we mounted the cov-erslips on microscope slides using Fluoromount and examined and ... result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing gene expression, cell growth and BMP-2 in gene expression and mineralization of bone.Materials and ... BMP-2, the yin and yang of mineralization:Contrast of effect of 24 hours of treatment with FGF-2 or BMP-2 on fold increase in abundance of mineralization-related geneexpression. Mineralizing...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf
... Li4AbstractIntroduction: The difficulty in re-growing and mineralizing new bone after severe fracture can result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing ... visualized the mineralizingcells with 2% Alizarin Red. After rinsing in distilledwater and air drying the samples, we mounted the cov-erslips on microscope slides using Fluoromount and examined and ... BMP-2, the yin and yang of mineralization:Contrast of effect of 24 hours of treatment with FGF-2 or BMP-2 on fold increase in abundance of mineralization-related geneexpression. Mineralizing...
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Báo cáo khoa học: "The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells" ppsx
... rationale for the use of taxol in DC immunotherapy and insights into the development of combinational DC therapies with other anticancer drugs that do not harm the viability or activity of DCs.Acknowledgments ... cells, producing major cytokines involved in immune responses. Importantly, taxol increased the expression of the anti-apoptotic protein Bcl-xL, and increased the mobilization of p65 subunit ... jooh@jejunu.ac.kr The role of Bcl-xL and nuclear factor-κB in the effect of taxol on the viability of dendritic cellsMi-Hyoung Kim1, Hong-Gu Joo1,2,*1Laboratory of Veterinary Pharmacology, College of...
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- the role of teachers schools and communities in quality education a review of the literature
- the role of supraspinal gaba and glutamate in the mediation and modulation of pain
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- the role of blade buoyancy and reconfiguration in the mechanical adaptation of the southern bullkelp durvillaea
- 2005 the role of teachers schools and communities in quality education a review of the literature academy for educational development global education center
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