... also increasingly involved inthe development and delivery of a range of education and training programmes designed to help individuals working in HIV advance professionally, from administering the ... activists and others working on the frontlines ofthe response The IAS encourages all professionals working inthe HIV/AIDS field to become members We are convinced that inthe struggle against this ... Providing education and training opportunities for its membership is important for any professional society, andthe IAS is looking at ways to strategically expand itsrolein this area in order...
... inthe KIND2 domain of v-KIND contain the core MAP2 binding module To identify the core MAP2-binding site within the KIND2 domain (amino acids 456–620), we analyzed five EGFP-fused KIND2 domain ... inthe central domain of MAP2 contain the v-KIND binding core module MAP2 consists of three main structural domains: the cAMP-dependent protein kinase regulatory subunit RII binding domain, the ... 1B) The results obtained indicate that the KIND2 domain is necessary and sufficient for the targeting of v-KIND to dendrites of neurons To investigate the MAP2-binding characteristics ofthe KIND...
... partitioning ofthe protein into an aggregated state The effect of copper binding on the secondary structure was determined using CD inthe far UV To prepare proteins without Cu2+, part of each protein ... understanding what causes the loss of appropriate metal binding might be crucial for the understanding oftheroleof amyloidogenic proteins in a number of neurodegenerative disorders Copper binds ... copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable of domain swapping and forms...
... concerning the kinetics and properties ofthe enzyme More strikingly, we show a novel roleofthe PPase enzyme inthe development and molting process of A suum larvae in vitro constructed in UniZap ... immunohistochemical staining), and that the inhibition of PPasecatalyzed PPi hydrolysis by these inhibitors, is likely to prevent the synthesis ofthe new cuticle from the hypodermis and/ or ecdysis ofthe old ... presence of increasing concentrations of inhibitors for 10 days, andthe number of molting larvae was determined Molting was manifested by shedding ofthe L3 cuticle Results Identification of cDNA...
... VBARP and Vpr is specific The input panel represents the amount of protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction ... transmembrane domains in VBARP Also, the use of unsynchronized cells in these analyses further confirmed that the cytoplasmic distribution of VBARP is independent ofthe cycling stage ofthe cells Identification ... modification and domain distribution of VBARP-L translated amino acid sequences The grayshade regions inthe amino acid sequence of VBARP-L protein represent the presence of ankyrin (ANK) repeats, amino...
... mislocalization of all three enzymes in these cells clearly shows the involvement of PEX14 inthe targeting of PTS-1, PTS-2 and I-PTS proteins to the glycosome To further analyse the effect ofthe destruction ... with a binding constant Kd of 2.75 lM, and that the amino-terminal domains ofthe two peroxins were responsible for this interaction Theroleof TbPEX14 in glycosome biogenesis received further ... N-terminal domain ofthe polypeptide of each of these organisms is followed by a stretch of hydrophobic amino acids that is presumably involved inthe protein’s anchoring to the membrane Also in...
... function inthe brain BGT-1 is capable of transporting both betaine and GABA, as examined in Xenopus oocytes and various cell lines But the physiological substrate of BGT-1 inthe brain remains to ... The biosynthesis of taurine is primarily from cysteine derived in part from methionine via the cysteine sulfinate pathway, which includes oxidation of cysteine to cysteine sulfinate by cysteine ... addressed the question of what is the normal distribution and subcellular location of BGT-1 inthe brain The use of a specific antibody to BGT-1 detected the presence of BGT-1 inthe cell bodies and...
... expression of PLA2 isoforms, or their physiological roles in specific regions ofthe brain and CNS The present study was carried out in view ofthe importance ofthe importance of PLA2 andits products ... Expression Profile of various PLA2 isoforms inthe CNS CHAPTER EXPRESSION PROFILE OF VARIOUS PLA2 ISOFORMS INTHE CNS 29 Chapter Expression Profile of various PLA2 isoforms inthe CNS 2.1.1 Introduction ... hippocampus including CA1-3 fields, dentate gyrus and brainstem (Shirai and Ito, 2004) The significance of PLA2 in maintaining membrane lipid homeostasis ofthe brain is evident in all these studies...
... violet The dots are stained cells on the bottom ofthe Matrigel-coated inserts The number of invasive cells was determined by counting the number of stained cells and then normalizing it to the ... OccWT-overexpressing cells, and that 2-APB inhibited the effects of OccWT Therefore, the increase in intracellular calcium may explain the induction of apoptosis and reduction of invasiveness mediated ... mechanisms of this regulation On the basis ofthe above findings, our research provides new insights into theroleof exon inthe regulation of apoptosis and invasion in liver cell lines FEBS Journal...
... using 2014 crotonyl-CoA as a substrate [15] The gene encoding this bifunctional protein was named AUH (‘AU binding homolog of enoyl-CoA hydratase’) The RNAbinding activity ofthe human protein ... factor of 20 over the dehydration reaction (kcat ¼ 0.26) which is consistent with the main roleof AUH in leucine catabolism, the hydration of 3-MG-CoA Comparing the turnover numbers of glutaconyl-CoA ... bovine serum albumin and 10 lm 3-MG-CoA or glutaconyl-CoA After incubation at 37 °C for 60 min, the reaction was terminated by the addition of 2.5 lL of m HCl The samples were homogenized, and the...
... satisfied with their circumstances of living, - and their average QOL domain scores would be high, in comparison with the international data - In view ofthe robust findings inthe literature, ... model The domain scores ofthe WHOQOL-Bref can be computed in three ways The first is a summation ofthe raw scores ofthe constituent items The second and third ways consist of transforming the ... friendly have the potential to improve the QOL of students These findings complement the results of our regression analyses The finding about the predictive power ofthe child’s perception of parental...
... they are homologues of host genes The most obvious of these is the collection of genes that are involved in altering or maintaining photosynthetic function ofthe host The most well studied and ... enzyme inthe synthesis ofthe polyamines spermidine and spermine With polyamines implicated inthe stabilising the psbA mRNA inthe cyanobacterium Synechocystis [64], altering structure of PSII ... using bioinformatic approaches, they are compared to T4 and their function is deduced on the basis of known genes inthe T4 genome In order to really progress with understanding theroleof the...
... andthe silastic catheter was inserted into the abdominal cavity for the continuous infusion of LPS at a rate of μL/hour for 20 hours (total dose: μg/kg BW) Following the closure ofthe incision, ... shown that the enzyme is involved inthe control of thyroglobulin folding and multimerization, probably by reducing the intermolecular disulfide bridges and thus limiting the extent of multimer ... molecular chaperones inthe folding of oxidized proteins Refolding of colloidal thyroglobulin by protein disulfide isomerase and immunoglobulin heavy chain-binding protein J Biol Chem 2001, 276:21337-21342...
... cleavage of LTBP at its protease sensitive hinge region, which is between the N-terminal ECM binding domain andthe C-terminal LAP binding domain This can be achieved by many proteinases inthe serine ... Val479 and Tyr583 are involved both in GDP and ATP binding to TG2 The residue Arg580, which is important for GTP/GDP binding, is not involved in ATP binding (Han et al 2010) By purifying the insulin-like ... be involved in integrin-dependent signal transduction, including inthe focal adhesion kinase (FAK) and GTP-binding proteins Raf-1 and Rho regulated signaling pathways (Hang et al 2005) These interactions...
... heme-binding regions in HRI: an N-terminal domain of HRI andin an insert region inthe protein kinase domain of HRI (63) Heme, inthe presence of iron, binds to α and β globin chains in ratio of ... HRI then phosphorylates eIF2 and inhibits protein synthesis Therefore, globin protein synthesis is reduced during heme deprivation andthe balance between the levels of globin protein are retained ... bold and a flanking purine at the -3 and a G at the +4 positions (15) Together the eIF1 and eIF1A facilitate the recognition and selection of initiating codons eIF1 plays a key roleinthe fidelity...
... translated amino acid sequences of GGT proteins in different H pylori strains possessing different level of GGT activities Determining the subcellular localization of GGT in H pylori Examining the pathway ... of ammonia and indirectly by urease-induced stimulation ofthe inflammatory response, including recruitment of leukocytes and triggering ofthe oxidative burst in neutrophils Survey of Literature ... encoded inthe cag pathogenicity island Following the transfer, the tyrosine residues ofthe CagA are phosphorylated by the host cell Src-like protein tyrosine kinase This leads to the regulation of...
... by the GTPase activating proteins Inthe cytosol, the GDP dissociation inhibitor binds to the GDP-bound Rab and inhibits the exchange of GDP for GTP, which would reactivate the Rab protein Bioinformatical ... g/mL of poly-L-lysine, were placed inthe same plate For the first 24 hours, the neurons were maintained in plating medium Thereafter, for the next 7-9 days, the neurons were maintained in Neurobasal ... important rolein myelin formation and maintenance in oligodendrocytes 26 Aims and rationale of current work 1.3 Aims and rationale of current work One ofthe main research focuses ofthe lab is...
... According to the type of amino acid phosphorylated, the protein kinases can be divided into two subgroups: protein serine/threonine kinases and protein tyrosine kinases Serine/threonine protein kinases ... domain is missing from the Ca2+-independent PKCs (nPKCs and aPKCs), but the V0 domain of nPKCs and aPKCs andthe HR2 domain ofthe PRKs are related to the C2 domain through the investigation ofthe ... comparison ofthe protein architecture ofthe various subgroups ofthe PKC superfamily The C1 domain ofthe aPKCs is smaller than that ofthe cPKCs and nPKCs as it contains only one copy ofthe zinc-finger...
... chlorine to monochlorine has been shown to alter the stability of PbO2 and trigger high levels of lead released into drinking water Lin and Valentine [21] investigated the reduction of PbO2 inthe ... lead into drinking water Since PbO2 plays a critical rolein regulating lead contamination in drinking water, a precise and fast method for its detection is required to determine its abundance in ... solids, and (3) explore the stability of PbO2 in chloraminated water inthe presence of Br- to advance our understanding oftheroleof PbO2 in lead release in drinking water Firstly, a modified iodometric...