... decided again and again thatit was nothing, but just as often they again began wondering what it was.And the fear of making themselvesridiculous kept them of speaking of it to John.Jack's ... rear end of an aisle which ran nearly the length ofthe room, behind the rank of tellers' cages and in front ofthe vaults. At the other end ofthe aislewas the door which opened on the two ... to the man who satwaiting. All the while the Judge had been hailing down a shower of small remarks upon all conceivablesubjects, and John had answered all of them ina voice that gave no hint...
... disRAS1fwd5¢-TTCACGATTGAACAGGTAAACAAAATTTTCCCTTTTTAGAACGACATGCAGCTGAAGCTTCGTACGC-3¢ and disRAS1rev CAAAACCATGTCATATCAAGAGAGCAGGATCATTTTCAACAAATTATGCATAGGCCACTAGGGATCTG-3¢. YEp351-SUT2 wasconstructed to contain SUT2 as the only open readingframe present inthe plasmid in ... http://mips.gsf.de/proj/yeast/info/tools/hegemann/gfp.html) using the primersSUT-GFPfwd 5¢-GACTGTCGATGATTATGGTTGCCCGCTGGCTTCCAAACCCTTATCGATACCGTCGACCC-3¢ and SUT-GFPrev 5¢-AACAATTTCACACACAGGAAACAGCTATGACCATGATTACGCTATAGGGCGAATTGGGTA-3¢, ... for RAS1 utilizing the S.pombehis5+construct amplified from plasmid pUG27 asdescribed above using the primers disRAS1fwd5¢-TTCACGATTGAACAGGTAAACAAAATTTTCCCTTTTTAGAACGACATGCAGCTGAAGCTTCGTACGC-3¢...
... Because the Asp214 of maltase is equivalentto the Asp215 of isomaltase, a mutant with the residuealtered to Ala was tested for its activity on a- pNPG.None ofthe mutants including D21 5A had ... cloning into plasmid pKP1500. The forward p rimer 5¢-ATGACTATTTCTTCTGCACATCCAGAGACAGAAC-3¢ con tains the initiation codon,while the reverse primer 5¢-CTTTCTGCAGACTCATTCGCTGATATATATTC-3¢ linked a ... then one da y later several b luecolonies appeared. Oneofthe clones expressing isomaltasewas selected. The plasmid containing the isomaltose genewas designated pYIM.Cloning ofthe maltase...
... 6: The graph of micro-average F -measureagainst the number of training sentences duringtext chunking (A: MHHMM, B: HHMM and C:HMM) The first finding is that the size of training datadramatically ... The seconddataset is part ofthe Lancaster Treebank corpusand contains 1473 sentences. Each sentence con-tains hand-labeled syntactic roles for natural lan-guage text. A. 200 A. 400 A. 600 A. 800 A. 1000 A. 1200 A. 14000.860.880.900.920.94B.200B.400B.600B.800B.1000B.1200B.14000.860.880.900.920.940.860.880.900.920.94FC.200C.400C.600C.800C.1000C.1200C.14000.860.880.900.920.940.860.880.900.920.94FFigure ... rolestypically has poor accuracy. Inthe text chunk-ing task the number of observation symbol relieson the number of part -of- speech tags contained in training data. Figure 6 plots the relationship of micro-average...
... P reviousinvestigations have demonstrated that tx 5a contains a disaccharide composed of N-acetylgalactosamine ( GalNAc)and galactose (Gal), but the interresidue linkage was notcharacterized. ... (DIPEA) in NMP. Toavoid diketopiperazine formation, Fmoc-Ala-Ala (Bachem,Torrance, CA, USA) was coupled as the first Fmoc aminoacid. A two-fold excess of Fmoc amino acids was used in the coupling ... linkage carbon. Together thesedata suggested that the interglycosidic linkage betweenGalNAc and Gal was 1–3 inthea lpha configuration. Thesedata were confirmed by the strong NOE between the 1Hatposition...
... mutations inthe binding domain of a- 2-C on the binding to c¢-2To elucidate whether single amino acids inthe bindingdomain of a- 2-C were particularly important for the formation ofa stable complex ... proline- and alanine-richlinker peptides. These linker peptides may allow hingemovements ofthe association domain against the carb-oxyltransferase and the biotin domain. The dynamics of conformational ... part and the biotin-binding domain inthe C-terminal part [5]. The b-subunit is an integral membrane protein with ninemembrane-spanning a- helices and a fragment insertinginto the membrane but...
... institutions in Havana, with ample fundsand well managed. Such are the Casa Real de Beneficencia, the Hospital of San Lazaro and the FoundlingHospital, Casa Real de Maternidad. In other parts ofthe ... Sabbath scenes in Havana Devotion ofthe common people The Plaza de Armas City squares The poor man'sopera Influence of music La Dominica The Tacon Paseo The Tacon Theatre The Cathedral ... price asked by them for an article, as they usually make allowances for beingbeaten down at least one half. The ladies commonly make their purchases inthe after part ofthe day, stopping in their...
... analyzed using the same software and hardware located at the central server location in New York. All MCG analyses in this da-tabase have been validated against the final medical and angiographic ... before the data analysis was carried out. The quality ofthe tracing was visually rechecked and graded as “good,” “mar-ginal,” or “poor”. A poor tracing was defined by one of the following: ... segments of ECG data containing baseline artifact that deviated from the baseline by ≥2 mm and appears ≥10 times, • two or more 5.12-second segments of ECG data containing baseline artifact that...
... half-life of PAI-1 was finally calculated from an exponential decay plot of the data obtained. Generally, only one preparation of each PAI-1 variant was investigated, but thefollowing wereinvestigated ... (Fig. 5). Accordingly, we assume that the aliphatic moiety ofthe introduced K in PAI-1(M149K)allows the side chain to adapt the same orientation as the original M and therefore the stabilizing effect ... at the various time-points, i.e. the fraction ofthe total amount of PAI-1 forming a stable complex with uPA, was calculated from the amount of PAI-1 required to inhibit half the uPA. The half-life...
... Registry of Societies profile printout ofthe Club/Association/Organisation or other certificate issued by the relevant regulating authority and original NRIC (Singaporean, Singapore PR and Malaysian) ... Customs Value [or commonly known as Open Market Value (OMV)] ofthe car by taking into account the purchase price, freight, insurance, handling and all other charges incidental to the sale and delivery ... (LP), a copy ofthe Accounting & Corporate Regulatory Authority (ACRA) profile printout ofthe Company/Business/LLP/LP, which is valid up to 14 days from the date of issue, and original NRIC...