... liquid -phase microextraction There are several parameters that affect the performance of LPME, namely the pH ofthe aqueous solution, the type ofthe polymer-based hollow fiber andthe type of organic ... favor ofthe deionization of analytes and to facilitate their distribution to the organic phase With the knowledge ofthe pKa value(s) of analytes would allow us to manipulate the acidity ofthe ... low viscosity of chloroform impeded the stability ofthe organic phase immobilized in the hollow fiber pores due to dissolution of chloroform in the midst of extraction n-Octanol was the only solvent...
... before sample injection to reduce the length ofthe concentrated sample zone and increase the peak height by slowing down the forward movement ofthe neutral sample associated with β-CD-NH2 and ... β-CD-NH2 andthe backward movement ofthe neutral sample partitioned in the micelles of sodium dodecyl sulfate (SDS) Stability ofthe inclusion complexes formed between the carrier andthe solute ... studies of many groups of compounds The improvement of sensitivity andthe miniaturization of CE technique are two major trends of capillary electrophoresis which have gain more and more attention...
... presents one ofthe simplest and most effective means of influencing the EOF ofthe separation system Normally the EOF increases with decreasing buffer concentration and enables analysisof highly ... than the EOF as their velocity direction is the same as that ofthe EOF, so very rapid analysis times are therefore achieved with positively charged compounds The neutral compounds move at the ... So the viscous drag ofthe solvent andthe charge and size ofthe solute thus control the migration of a species in an applied electric field The separation efficiency, normally expressed as the...
... assessment ofthe trajectory of interconversion rates as a function of time of cold exposure, (b) comparison ofthe magnitudes ofthe various interconversion rates, and (c) lineup ofthe accessions ... photosynthesis, gas exchange of plants was measured by infrared gas analysis Measurements were performed during the first h 508 ofthe light phase, representing the time period of photosynthetic ... invertase activity The control of each assay was boiled for after addition of enzyme extract Reactions were incubated for 60 at 30 and °C, and stopped by boiling for min; the concentration of glucose...
... and, in the case ofthe sample uptake tubing, care must also be taken to avoid contamination ofthe sample through contact with the tubing material For routine analysisof organic solvents, the ... analysisofthe organic solvent is the preferred method of analysis, either untreated or simply diluted in a suitable solvent Direct organic solvent analysis demands some specific features and ... to the plasma, the plasma environment becomes considerably more reactive, and so the use of platinum-tipped interface cones instead ofthe standard nickel cones is recommended Optimization of the...
... of each sample The numbers forthe base positions that are reported here are the bases compared to the positions ofthe full length genome of HCV-N [44] Control HCV sequences for strains 1a and ... transmissions, and retransmissions of HCV JGP performed the clinical work, recruitment of patients, and procurement of specimens DR, MOA, RSB, DB, and NC performed the molecular work Acknowledgements The ... sequences of PCLB-T7, and 19 sequences from 081 serum were identical Five ofthe sequences from the 081 serum and eight from PCLB-T7 had one change from the consensus, while five ofthe PCLB-T7 and...
... substance They are then transferred to a medium free ofthe test substance forthe depuration phaseThe concentration ofthe test substance in the fish is followed through both phases ofthe test ... consists of two phases: the exposure (uptake) and post-exposure (depuration) phases During the uptake phase, separate groups of fish of one species are exposed to at least two concentrations ofthe ... Without internal standardization, ageing ofthe SPME fiber is challenging, as is the variation ofthe MS performance Since a correction method for routine analysis is missing for MS, the alternative...
... reflectance in the visible yet reduced reflectance in the NIR and SWIR regions ofthe spectrum Analysis of vegetation spectra 519 Figure Summary of F-ratio tests of significance ofthe variance ofthe ... and tree effects In the eucalypt data they arise due to plot (site) and tree effects The expected power spectrum for a given group is one for which the random effects are zero Estimation ofthe ... where σ ijk (ϖ | ν k ) is the variance ofthe reflectance ofthe jth foliage sample in the ith group (treatment × foliage age forthe balsam fir samples, and species forthe eucalypt samples) at...
... means ofthe comparison ofthe RAPD marker frequencies of a subsampling of these progenies andofthe adult population Analysisofthe morphological characters MATERIALS AND METHODS Sampling The ... Morphological analysis In the second case ofthe figure, the heterozygote deficit (f) must be considered The frequency ofthe phenotype A+ is the sum ofthe frequency ofthe genotype P : +/+ All ofthe ... situated on the side ofthe species in the majority represented in the neighbourhood, the sessile oak The comparison ofthe mean values ofthe groups on the first axis of DFAa by means ofthe F-test...
... the induction of MMP-1 and MMP-3 in cartilage and SFB by TNFα and/ or IL-1β [42,43] Therefore, they support the validity ofthe new model fortheanalysisofthe mechanisms of cartilage destruction ... supplemented FCS The MMP suggested on the basis of their known caseinolytic activity andthe molecular weight of their latent and active forms were then identified by western blot analysisofthe same ... may allow the testing/screening ofthe molecular basis and efficacy of new therapeutic strategies and thereby contribute to the improvement of anti-rheumatic therapy Competing interests The authors...
... on the other hand, is homozygous forthe « high » allele (A All ofthe l ) l progeny from the standard cross have inherited the A allele from the father and are, therefore, heterozygous A The ... of 2.0, andthe l other allele (A had no effect on the base phenotype of 10 The sampled population ) had allelic frequencies of A l A 0.5 A total of 25 isofemales were randomly sampled from the ... set of offspring therefore carries a limited sample ofthe genetic variation segregating in the original population If mating is at random with respect to the polygenic loci of interest, the...
... responsible forthe surgical part ofthe investigation CM and ARR carried out the LIFE/DEAD-assay and 2-photon microscopy PZ was involved in the design ofthe study, evaluation ofthe results and covered ... covered the clinical part EV was involved in the design ofthe study and histomorphological evaluation TG conceived the study and was involved in the evaluation ofthe data as well as the writing of ... viability and functionality ofthe cells Furthermore, specimens of breast cancer were also treated like the lung tumor samples to verify the efficiency ofthe used drug concentrations on the chosen...
... cutoff; andfor option the user is prompted to provide the number of simulations andthe significance P value cutoff to be used in the permutation analysis Any region with a P value lower then the ... width of portion ofthe peak above the significance cutoff, 'array density' ofthe peak, andthe P value for that peak The array density value is defined as the average number of arrayed elements ... with which the user is comfortable The "Significant Negative Regions" sheet provides an empirical estimate ofthe number of false-positive findings forthe selected P value cutoff, and so the user...
... with the technical aspects of data import and management, and refer the interested reader to the documentation ofthe software package prada for a thorough discussion of these [19] Selection of ... cleaning up ofthe data, removal of systematic biases and drifts (a process often referred to as 'normalization'), and transformation to a format and scale that is suitable forthe following analysis ... (SSC), and they measure cell size and cell granularity (the amount of light-impermeable structures within the cell) One ofthe fluorescence channels monitors emission from the YFP tag ofthe overexpressed...
... this case, the variances ofthe classes are pooled to compute the standard error, s2 and s2 denote the variance ofthe two classes respectively, N1 and N2 denote the sample size ofthe two classes ... Tk be the ordering of k genes sorted in increasing order ofthe modified t-statistic The permutation test randomly swaps the class labels ofthe original data, preserving the proportions ofthe ... microarray andthe number of ways of sampling (n − k) non-DEGs in the pathway from (N − K) non-DEGs in the microarray, andthe denominator is the number of ways of sampling n genes in the pathway...
... if the class andthe feature are independent, the value of mutual information is equal to zero Hence, the greater the values of information gain, the higher the correlation between a feature and ... as both the predictions of Gaussian Processes for Classification, (3.12) and ARD, (3.18) require the use of MCMC We will only discuss it briefly For details of MCMC, the theorems and proofs can ... interaction, single strands from targets andthe single strand ofthe cDNA will combine andthe targets will stick onto the immobilized probes, binding the targets to the microarrays In other words, such...
... unsuitable for mass production because ofthe inherent serial nature ofthe system36 With the numerous benefits andthe wide variety of substrates and techniques available for microfabrication of microchips, ... potential and η is the viscosity ofthe buffer The buffer parameters are affected by the composition ofthe buffer used, its pH as well as the type of organic additives introduced For instance, when the ... even after it is deformed by the application of external forces Hence, soft lithography technique is commonly utilized forthe microfabrication of such microchips35 The design ofthe fluid circuit...
... liquid form and permits the direct use ofsolidphase extraction materials in theanalysisofsolid samples 1.2 Scope of our project The main objectives of this work are to improve sensitivity of ... funnel for extraction and at the same time as a syringe for direct injection ofthe extract into a GC or GC-MS foranalysisThe main feature of this method was the use of smaller amounts ofthe ... enhancement ofthe partitioning of analytes from donor phase to acceptor phase; another is the introduction of salt These two functions can prevent the loss ofthe organic solvent acceptor effectively The...
... employ the internal standard at the end ofthe procedure (to an aliquot ofthe final extract) assuming the volume ofthe organic phase as being exactly 10 mL It should be furthermore noted that the ... lost The extent of losses depends on the amount of lipids in the sample as well as on the polarity ofthe analytes with the most non-polar ones showing the highest losses In the presence of elevated ... Mini-Multiresidue Method fortheAnalysisof Pesticides Page of 12 The pH-adjusted extract is filled into vials for gas- and liquid chromatography and is used for further analysis Annotations 6:...
... implanted forthe relief of severe lower limb spasticity and flexor spasms The goals then are improved comfort and wheelchair posture, Intrathecal baclofen forthe control of spinal and supraspinal ... the process of consideration for ITB Patients may hear about ITB and discuss the question with their therapists rather than their doctors The charting of range and function before and after a ... cord than in the brain because ofthe characteristics ofthe circulation ofthe CSF from the ventricles to the spinal subarachnoid space The effective distribution volume of intrathecally administered...