role of neuropeptide y on the maintenance of self renewal and proliferation of human embryonic stem cells
The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... form all the 200 or so cell types in the body There are four classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma ... stem cells versus embryonic stem cells The general differences between the characteristics of adult and embryonic stem cells are summarised in Table The contention that somatic stem cells alone ... murine) leads to the 19 development of an embryonic stem cell line In nature however, embryonic stem cells are ephemeral and present only in the ICM of blastocysts The cells of the ICM are destined...
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ROLE OF THE ERP29 IN MIGRATION, APOPTOSIS AND PROLIFERATION IN BONE MARROW STEM CELLS
... Materials and Methods 26 Materials and methods 2.1 Isolation of Rat Bone Marrow Stem Cells The Bone Marrow Stem Cells (BMSCs), a type of mesenchymal stem cells extracted from the femurs and tibias of ... degradation by NK cells (Uccelli et al., 2008) In addition, BMSCs have the ability to suppress of B cell proliferation by blocking the G0/G1 phases of the cell cycle, and reducing the expression of ... The degree and direction of differentiation vary among individuals because of the variation of method and extent of induce Lastly, there is persistent doubt whether the BMSCs differentiated cells...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton Biol Chem 2006;387(4):401-6 Lopez LA, Bertini F The in vivo effect of low body temperatures on the degree of polymerization ... application to proliferation and cytotoxicity assays J Immunol Methods 1983; 65: 55-63 Heng BC, Liu H, Rufaihah AJ, Cao T Human embryonic stem cell (HESC) colonies display a higher degree of spontaneous ... 2000;18(4):399-404 Richards M, Fong CY, Tan S, Chan WK, Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... transcriptional network for pluripotency of embryonic stem cells Cell 2008, 132(6):1049-1061 Heo JS, Lee MY, Han HJ: Sonic hedgehog stimulates mouse embryonic stem cell proliferation by cooperation of ... Correlation coefficients of miRNA-target gene pairs The expression of miR-302c and miR-502b and their predicted target genes was analyzed by correlation analysis The distribution of the correlation ... cells by microarray analysis, and by qRT-PCR its expression was increased by only 3-fold in hES cells compared to EB cells and 25-fold to adult cells This discrepancy may have resulted from the...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; H3K36me3, trimethylation of lysine 36 of histone H3; MacroH2A, histone variant MacroH2A; meC, methylcytosine ... represents hydroxymethylation Unique DNA methylation patterns Another, previously unappreciated modification of DNA, hydroxymethylation, has become a subject of considerable attention DNA hydroxymethylation ... pluripotent stem cell; H2AZ, histone variant H2AZ; H3ac, acetylation of histone H3; H4ac, acetylation of histone H4; H3K4me1/2/3, (mono-, di- and tri) methylation of lysine of histone H3; H3K27ac, acetylation...
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Baculovirus mediated genetic modification of human embryonic stem cells
... transgene expression cassette VII Finally, the effects of the baculoviral transduction of the human embryonic stem cells were investigated The transduction of human embryonic stem cells by baculovirus ... course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell research began with the ... transient and stable expression in human embryonic stem cells for the purpose of genetic modification, thus facilitating the realization of the great potential of these cells in basic researches and...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... observation is the expression of VE markers which initially mark the cells overlying both embryonic and extraembryonic regions and are subsequently restricted to the visceral layer of the yolk sac ... established by the expression of relevant markers like CD45, CD14, CD34, CD83 and CD86 and the formation of colony forming units in methylcellulose cultures The combination and concentrations of the cytokines ... development in the mouse In the mouse, haematopoietic lineages first appear in the form of blood islands in the yolk sac, a derivative of the extraembryonic mesoderm by embryonic day E7.5 of gestation (Russell...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity to self renew and can ... Although, these cells have a high capacity to self renew in culture, their ability to expand is less than that for embryonic stem cells The self renewal and the proliferative capacity of these stem cells ... hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells Adult stem cells can be derived from various parts of the body including...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... Table of Contents II Summary V List of Tables VII List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic ... characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst stage (Martin, 1981; Evans and Kaufman, 1981; Thomson et al., ... via self- renewal by the prevention of differentiation and the promotion of proliferation under proper culture conditions (Niwa, 2007) Figure ES cells two defining features: self- renewal and...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and ... result of chordin knockdown in cells from all donors tested Another factor to consider is whether the age of the donors had an influence on the results of this study Four donors were over 70 years ... years old and one donor was aged 19 The relative increase in expression of the osteogenic markers in the young donor, as a result of chordin knockdown, was within the range of that of the donors...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... chondrogenesis of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of ... and CS4, one of the monomers of the polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture in the aggregates ... calcifying zone of cartilage could be clearly separated by the proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of...
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Báo cáo y học: " Effect of neutrophil elastase and its inhibitor EPI-hNE4 on transepithelial sodium transport across normal and cystic fibrosis human nasal epithelial cells" pot
... the study were therefore to test the effects of hNE and EPI-hNE4 on ENaC activity and transepithelial Na+ transport in vitro in primary cultures of human nasal epithelial cells from control and ... airway inflammation, and that the stimulatory effect of hNE on ENaC could be unmasked On this condition, the use of EPI-hNE4 could be of interest to reduce ENaC hyperactivity in CF airways In ... tested the effect of hNE on transepithelial Na+ transport in control and CF HNEC monolayers As shown in Figure 1, increasing concentrations of hNE (final concentration in the apical bath: 10 and...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... within the LCR and was only detected at the core regions [14,15] Localization of RNA Pol II to the LCR was independent of active transcription elongation; the addition of the elongation inhibitor ... containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes but not the embryonic and adult globin genes (Fig 2) Upon differentiation the embryonic- and adult-specific b-globin ... Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... cardiomyocytes, as demonstrated recently by three groups in the mouse ESC system [68-70] Simultaneous isolation and characterization of functionally distinct colonies and analysis of gene expression ... multi-comparison analysis is demonstrated by the consistent identification of a set of genes among multiple comparisons, of which a subset of genes were confirmed by immunocytochemistry For immunochemistry ... analyses The reliability of the microarray data generated from our multi-comparison analysis is demonstrated by the consistent identification of a set of genes among multiple comparisons, of which...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... A, Conget P 2001;Mesenchymal stem cells Exp Biol Med (Maywood) (6):507-520 115 Aratani Y, Kitagawa Y 1988;Enhanced synthesis and secretion of type IV collagen and entactin during adipose conversion ... regulation of niche, self- renewal and differentiation Arthritis Res Ther (1):204 121 Owen M, Peck W 1985;Lineage of osteogenic cells and their relationship to the stromal system In ed Bone and MineralAmsterdam ... alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies Evidence that the synergy sequence of fibronectin is recognized by the amino-terminal repeats of the alpha5 subunit...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... pathway is one of the key pathways that control the development of the central nervous system in mammals It is also important in the development of many other organs such as the limbs, bone, lung and ... Preparation of conditioned media from !E-MEFs 33! 3.2.3! Human embryonic stem cells and induced pluripotent stem cells 33! 3.2.4! Embryoid body formation 33! 3.2.5! Generation of...
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Atlas of Human Pluripotent Stem Cells doc
... embryonic stem cells: the birth of the first IVF test-tube baby in 1978 (Steptoe and Edwards 1978) and the discovery in 1981 by Martin (1981) and Evans and Kaufman (1981) of mouse embryonic stem cells ... stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for studying the fundamentals of ... paved the way to the derivation of the first five human embryonic stem cell lines by Thomson et al in Madison, Wisconsin, 1998 (Thomson et al 1998) More recently, Yamanaka and team enthused the scientific...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... recovery of impaired motor function by quantification of the time from the start of the exercise until collapse on an accelerating rota-rod just before the experiments (= day 0) and on day and 28 ... Functional endothelial cells derived from rhesus monkey embryonic stem cells Blood 2004, 103:1325-1332 Saeki K, Yoshiko Y, Nakahara M, Nakamura N, Matsuyama S, Koyanagi A, Yagita H, Koyanagi M, Kondo ... obtain only MCs derived from human ES cells (hES-MCs) for the transplantation (Figure 1) On the day of transplantation, these cells were washed with PBS twice and harvested with 0.05% trypsin and...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... ECM bind to cells by activating signaling molecules such as Akt and ERK Therefore, we analyzed the effect of FN and LAM on the phosphorylation status of Akt and ERK for stage III cells There was ... phosphorylation of AKT approximately 1.7-fold and 2.1-fold compared to the control, respectively (Figure 4B), and activated phosphorylation of ERK roughly 2.4-fold and 4-fold compared to the control, ... collection and assembly of data, data analysis and interpretation, and manuscript writing, LLC assisted with collection and assembly of data, data analysis and interpretation, SHC assisted with conception...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... formed by the fusion of mononuclear progenitors of the monocyte/macrophage family Therefore, it is a member of the mononuclear phagocyte series and may be thought as a specialized type of macrophage ... nanofibers was considerably lower than on the TCPS plates In Figures c and d, the expression levels of BSP and WNT5A of the cells on the nanofibers were significantly higher than on the controls ... between the clear zone and the resorption area of bone surface The ruffled border is the site where enzymes and acids are released to facilitate the degradation of matrix and the dissolution of minerals...
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