... valleys (valley bottoms plus valley fringes), determining the cultivation intensities ofinland valleys and their uplands, and studying the land-use characteristics ofinlandvalley bottoms and fringes, ... shown in Figure 8.6 in the color section) ofWestandCentralAfrica to characterize and map inlandvalleyagroecosystems The characteristics studied include: inventorying the areas ofinland valleys ... groundtruthing using a GPS Digital georeferenced databases generated include spatial distribution ofinland valleys and land-use classes ofinland valleys and uplands Save (Republic of Benin) study...
... part in explaining the ability of the cocoa sector in one country to compete with cocoa sectors in other regions inWestandCentralAfrica (Nkamleu 2004b) From a policy standpoint, the results of ... Agriculture in 2002 in Côte d’Ivoire, Ghana, Nigeria, and Cameroon A baseline survey of major cocoagrowing regions ofWestandCentralAfrica was conducted to provide baseline parameter estimates of production ... technique to investigate productivity potentials and efficiencies in cocoa production inWestandCentralAfrica Since technology is a representation of the state of knowledge pertaining to the...
... evolution of the poultry industry inWestandCentralAfricaand its causes According to FAO data, chicken meat production in West/ Central African countries has increased between 1996 and 2003 In CEMAC ... Furthermore, since the high prices for chicks and particularly for feed are the main problem of chicken production in Western andCentral Africa, and since locally produced frozen chicken would have a higher ... broiler production units inCentraland Western Africa An intensive and large-scale poultry production does in some Western andCentral African countries exist, as it does in the egg production...
... PiT1-binding immunoadhesin generated by fusing the aminoterminal residues of the GALV (SEATO strain) Env, comprising the signal peptide, the RBD and the proline-rich region, to the rFc tag, herein, ... significant differences in body weight between normal and intoxicated animals Regional GLUT1, PiT1 and PiT2 distribution in the brain of normal mice Cortex staining: GLUT1 staining was heterogeneous ... and PiT2 ligands PiT1 staining exhibited a linear pattern with few stained cells following the myelinated fiber bundles corresponding to oligodendrocytes (Figure 3B) Basal ganglia staining: In...
... determined in this study and those retrieved from the database were aligned using Clustal W (Thompson et al., 1994) Phylogenetic trees were constructed using Clustal W and Tree View (Page, 1996) using ... slope of the line describing the linear decrease in phosphate concentration The ratio of anoxic PUR to aerobic PUR (anoxic/oxic PUR ratio) was used as an index reflecting the fraction of DNPAOs ... retention time of wastewater in the sewer line (lower composition of fatty acids), and (iii) low nitrogen loading to the anoxic tank In this study, the nitrate concentration in the influent to the...
... of PC1-axio, suggest that 18:2n6c and LCFAs were contained in greater amounts in the roots and rhizomes than in the leaves and stems, and 18:3n3 was contained in lesser amounts in the roots and ... structure in Z japonica We found about 30 kinds of fatty acids in the leaves and stems and about 36 kinds of fatty acids in the roots and rhizomes of Z japonica We focused on the fatty acid content of ... Z.marina RR LS -14 -13 -12 -11 -10 -9 -8 δ13C(‰) Fig.2 Comparison of δ Z in Z japonica Fig - Comparison of δ13C in 13C japonica and Z marina and Z marina LS: leaves and stems LS: leaves and stems...
... findings argue for an early evolutionary origin and a high conservation of this class of proteins at both the structural and functional levels Given the multiplicity of CLPs in humans and insects ... transcripts during development andin adult tissues To gain insights into possible physiological functions of Cg-Clp2, determination of its tissue distribution and temporal pattern of expression during ... on the conserved amino acid sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence Cloning and sequencing of this fragment revealed...
... using 2014 crotonyl-CoA as a substrate [15] The gene encoding this bifunctional protein was named AUH (‘AU binding homolog of enoyl-CoA hydratase’) The RNAbinding activity of the human protein ... protein (AUHp40) Western blot analysis of brain extracts consistently revealed a 32 kDa AUH protein and it was thus assumed that the mature form of human AUH in brain has a molecular weight of ... For the kinetic characterizationof AUH described in the work at hand, AUH was overproduced in Escherichia coli as a maltose binding protein fusion (MBP-AUH) The complete AUH gene (producing MBP-AUHp40...
... (1988) Binding of sulfobromophthalein to rat and human ligandins: characterizationof a binding-site peptide Biochim Biophys Acta 955, 296–300 52 Gregus Z & Klaassen CD (1982) Role of ligandin as ... containing 29 lM BSP and increasing concentrations of sucrose After attainment of the steady state, lL (¼ lg) valinomycin was added Data (n ¼ 3) are means ± SEM and were fitted to a straight line ... found to linearly depend on the addition of protein (2.6 ± 0.04 lmolÆmin)1Æmg protein)1, with lg valinomycin), as well as of K+ (6.25 ± 0.16 unitsÆmEq)1 K+, with lg valinomycin) and valinomycin (0.51...
... goal of contributing to understanding of the metabolism of this pathogen and providing the enzyme for the identication and development of selective inhibitors, we cloned and expressed Rv2992c in ... the discriminating behaviour of Mt-GluRS in E coli Studies on Tt-GluRS indicated that discriminating and nondiscriminating GluRS can be distinguished on the basis of the presence of a specic ... exceptions in that activation of the amino acid requires the presence of the cognate tRNA [2,4] In these aaRS the binding of tRNA induces an ATP productive binding mode [5] GluRS are also distinguished...
... [19], and papain [11], VS-A, VS-B and VXH-A lack the insertion of four amino acids between position 168 and 169, which is present in all other cysteine proteinases of plant or animal origin Unfortunately, ... cysteine proteinases The N-terminal amino acid sequences of the proteins VXH-I, VXH-III, VXH-IVa and VXH-IVb are shown in Fig 6, and are compared with the known N-terminal sequences of the cysteine ... enzymes will be evaluated in the following experiments cDNAs coding for cysteine proteinases in V · heilbornii and V stipulata Using a PCR approach with a cysteine proteinase primer (CyPr), specifically...
... fluorescence of the flavin in HOX by acid hydrolysis might provide a novel and quick method to distinguish between this type of flavin-binding motif and other covalent-binding motifs The following line of ... according to the 2695 Characterizationof flavin in hexose oxidase T Rand et al apparent extinction coefficient of the flavin in the mutant enzyme (e437 ¼ 5.5 mm)1Æcm)1), only half of the flavin is incorporated ... position of histidine The higher pKa value is attributed to the in uence of the remaining free amino acids in the hydrolysate HOX, GOOX comparison According to the model of FAD in HOX presented in...
... model of infection, and with relative sensitivity to host factors such as complement mediated killing [36] Structural analyses of a majority of H in uenzae strains including strain Rd [8] and several ... the members of the Finnish Otitis Media Study Group at the National Public Health Institute in Finland for the provision of NTHi strains from the nasopharynx, obtained as part of the Finnish Otitis ... derivatives In addition, small amounts of glycine and traces of N-acetyl neuraminic acid (Neu5Ac) were detected as substituents of LPS by HPAEC following treatment of 0.1 M NaOH and neuraminidase,...
... kDa band in T cells and Jurkat cells and a 40 kDa protein band in Jurkat cells As no 40 kDa band could be detected using the anti-Cb(SNO157) in B C Fig Identification of PKA catalytic subunits in ... kDa band in T cells A Cb-specific antibody anti-Cb(SNO157) (vi) recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells (C) Linear depiction of Ca1, Cb1 and ... analogue was included to avoid potential binding of non-Cb2 C-subuints to the R-subunits The resulting precipitates were analysed by SDS ⁄ PAGE and immunoblotting using anti-Cmono (Fig 2B) and a PKA-specific...
... +22%) In turn, regional homogeneity is highest within the Netherlands, Austria and Hungary, when comparing production changes in the 20% least expanding and receding countries The increase in cow ... following the drop in fat and protein prices but still remaining above intervention (which was also reduced in the baseline 14 See Annex for some explanations on the balancing of fat and protein in ... expansion of 2% in 2008 for the EU-27 and also incorporates some decreases in production in the NMS due to a decline in subsistence production, mainly observed in Poland, Bulgaria and Romania12 In Table...
... divided spin coating into the four stages of deposition, spin-up, spin-off, and evaporation (Figure 2.31) By taking both the force of spinning and the concentration-controlled evaporation into account, ... interaction, and a polymerinterface strong interaction can be seen in Figure 2.2-2 This research aids in understanding the behavior of thin polymer films Free-standing films have increased mobility ... behavior using the intermolecular and intramolecular forces among polymer chains, was employed to understand the behavior of these thin films Another type of thin film, a polymer brush, was investigated...
... SDR family member Destrin (actin-depolymerizing factor) EF-hand domain containing protein 2, Swiprosin-1 Ezrin (p81) (cytovillin) (villin-2) Heat shock 27 kDa protein Integrin a-M precursor Lysosome-associated ... membrane staining of annexin II in G93A motor neurons Immunofluorescent staining of annexin II and the neuronal marker neurofilament M (NF-M) in spinal cord motor neurons in 90-day-old and 125-day-old ... majority of the proteins in the above two functional categories, i.e seven of 10 proteins involved in cytoskeletal regulation, and five of six proteins involved in microglia activation, showed higher...
... in intracellular cytoskeletal proteins, including titin, MyBP-Cm, MyBP-M, MyBPH, myotilin, and palladin [30–35] As Ig-C2 domains may serve as molecular spacers and bind to a diversity of ligands, ... domain in myosin light chain kinase A Fig Prediction for a conserved aggregation domain in the 4Ig region of L-MLCK and recombinant expression of MLCK variants (A) The sequences of the N-terminal ... barrier in intestinal disease Lab Invest 84, 282–291 12 Tran QK, Watanabe H, Zhang XX, Takahashi R & Ohno R (1999) Involvement of myosin light-chain kinase in chloride-sensitive Ca2+ in ux in porcine...
... gene in normal and malignant mouse melanocytes, in anagen and catagen skin, andin pituitary and spleen In telogen skin gene expression was low Expression of the TPH gene in skin andin skin-derived ... function of NAS in skin physiology In summary, we present the molecular and biochemical characterizationof the apparatus producing and metabolizing serotonin and N-acetylserotonin in the skin of C57BL/6 ... tryptamine and serotonin changed during hair cycling from approximately in telogen and anagen III to 16 and 17 in middle and late anagen VI, being 14 in catagen The same ratio was 1.5 in the ear and...
... right of each line The sequence of the N-terminal 25 amino acid residues (IEP-1) determined in the prior study [21] is underlined The amino acid sequence (10 amino acids) of IEP-2 determined in ... microscope RESULTS Finding of IEP homolog Fig Tissue distribution of the IEP proteins and corresponding mRNA (A) Western-blot analyses were performed with anti-IEP IgG Immunostaining of male and female ... eggs preincubated with anti-IEP IgG for 30 were injected into a day last instar larvae After 12 h, injected eggs were collected and observed under a microscope Cloning, sequencing andin vitro...