recent studies involving in situ blend formation using supercritical carbon dioxide
extraction of essential oil from lemongrass using supercritical carbon dioxide
... during the time I stayed and researched in Japan but also in the Philippines Thank you for kindly sharing your knowledge as well as experience in research, Thank you for cherishing and supervising ... as supercritical fluid chromatography In this process, SC CO2 has been used as a mobile phase Recently, there has been a significant increase in interest of the use of supercritical carbon dioxide ... your help during my studies at DLSU Prof Hitoshi Kosuge and Dr Hiroaki Habaki, TIT for your help during the time I stayed in Tokyo and for your advising in my research as well as in writing my paper...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
... detecting CHV in MDCK cells infected with 103 TCID50 using overnight hybridization protocol However, biotin-labeled probe was able to detect CHV in MDCK cells infected with 104 TCID50 By using ... hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe, 100 µg/ml proteinase K pre-treatment, and ... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated...
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báo cáo khoa học:" Recent technological developments: in situ histopathological interrogation of surgical tissues and resection margins" ppt
... that we have more informed choice of the state of the in situ epithelial margin taken when excising squamous cell carcinoma by having better information about the tumour margin This together with ... dimensional information concerning depth of invasion However this may be ascertained by a series of 'MOHR'S'-like dimensional analyses using the microendoscope A degree of depth of field information ... be determined This dynamic un-ruffling is seen in Figures 3, &5 We used 0.1% Methylene blue as our vital staining agent; this was determined by using a dilution series of various stains (Evan's...
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Báo cáo hóa học: " In situ Polymerization of Multi-Walled Carbon Nanotube/Nylon-6 Nanocomposites and Their Electrospun Nanofibers" pot
... profound shear-thinning behavior as the / increased Percolation threshold represents a starting point of a three-dimensional MWNT network in the matrix, and can be determined from the starting / at which ... were added to a 10 mL glass syringe using a needle tip with a 0.5 mm diameter The feeding rate was 0.2 mL/h, which was controlled by a syringe pump Electrospinning voltage, the distance between ... WAXS patterns in the nanofibers were due to the small size of the crystal [48] Conclusions We prepared two kinds of the nanocomposites by using an in situ polymerization method in the presence...
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Thermodynamics of cholesterol compounds in supercritical carbon dioxide experimental and modeling studies
... Graph showing the supercritical region of CO2 10 Figure 2.2 Variation in density of CO2 in the vicinity of its critical point 12 Figure 2.3 Diffusivity behavior of carbon dioxide in the vicinity ... acetate, butyrate and benzoate in supercritical CO2 Experimentally determined solubility isotherms in pure supercritical carbon dioxide were obtained over a range of operating conditions from 90 to ... cosolvent is usually introduced into the supercritical carbon dioxide to enhance its solvating power for polar organic compounds Numerous efforts in recent years have aimed at studying this solubility...
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extraction of liquid crystals from flat panel display devices using both liquid and supercritical carbon dioxide
... controllers) to PC monitors and increasingly in large area high definition display devices Although liquid crystals are non toxic,[3] they persist in the environment and with increasing concern for our fragile ... these highly toxic and potentially carcinogenic solvents are not viewed as a “sustainable” long term solution to the problem In recent years supercritical carbon dioxide (ScCO ) has proven to be an ... Following from this the first carbon dioxide extractions of defunct LCDs was demonstrated at the University of York, resulting in a worldwide patent application for this process.[8] Herein we...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 1
... science and bioinformatics (Donald and Mohammad, 2001) Chapter 1.1.1 Cells Tissue engineering utilizes living cells as engineering materials Examples include using living fibroblasts in skin replacement ... fiber scaffolds containing bone morphogenetic protein (BMP-2) and/or nanoparticles of hydroxyapatite (nHAp) using electrospinning, and showed its ability in inducing in vitro bone formation from human ... for tissue engineering applications using the electrospinning process Scaffolds generated by electrospinning contain nanoscale fibers with microscale-interconnected pores, resembling the extracellular...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 2
... Conclusions This study investigated two methods to load BMP-2 into three dimensional fibrous scaffolds using an electrospinning method, including encapsulating into fibers or coating on fiber surface, ... (for instance emulsification and electrospinning as well as post-processing conditions like scaffold handling and incubation) that may result in partial removal of amino acid groups and conformation ... F4 seems less integrated and this finding indirectly explains why BMP-2 in F4 was released much faster than all other fibers Chapter 52 Controlled and sustained release of protein is crucial...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 3
... BMP-2 into three dimensional fibrous scaffolds using an electrospinning method, including encapsulating into fibres or coating on fibre surface, and the results revealed that BMP-2 encapsulated into ... treatment of IHC staining, vWF can be stained as brown color Therefore brown color staining detected in bone fragments can be seen as the formation and growth of new blood vessels H&E staining analysis ... scaffolds obtained from the electrospinning method, 15mg of each sample of F1-F4 were weighed and dissolved in mL of DMF solution to extract DCM after freeze drying for days inside a Martin Christ...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 4
... a 10mL glass syringe (MICRO-MATE interchangeable 10cc hypodermic syringe, Popper & Sons, Inc., New Hyde Park, NY US) fitted with a 29-g needle and set up in the elecontrospinning apparatus The ... observed the corresponding strain Differential scanning calorimetry (DSC) Differential scanning calorimetry (DSC) can be employed to determine the amount of crystalline structure within the microfibers ... nanoparticles integrating well with the PLGA, adopting an efficient composite structure of inorganic-organic system as observed in natural bone The mechanical properties of the scaffolds fabricated using...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 5
... the lowest Interestingly, the concentration increased significantly in the subsequent two weeks, suggesting that the expression of DNA from group C samples was sustainable and lasting Nonetheless, ... arrows identify lacunae Original magnification is 100X for all Chapter 123 Besides H&E staining, IHC staining was also performed to check the formation of blood vessels in bone fractures After treatment ... level in the second week, implying that the cytoclast differentiation rate and bone healing rate in B3 were both higher than other samples Consequently, good healing result for B3 was detected in...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 6
... are maintained after going through the processes of spray drying, supercritical CO2 foaming and post-processing conditions (handling of scaffold, incubations, and lyophilization) As shown in Figure ... process (including spray drying, supercritical CO2 foaming, and handling of samples) on the molecular integrity of plasmid DNA, agarose DNA gel electrophoresis was utilized to determine the integrity ... 30 days retained its original structural integrity as evidenced by the distinct bands present on the gel (Figure 7.5b) This shows that the plasmid DNA integrity could be maintained in the course...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 7
... Lysine-based peptides modified foams for tissue engineering and DNA delivery Toward realizing an effective dual system for tissue engineering and DNA delivery, our work presents intriguing findings ... N-methylmorpholine The removal of Fmoc was completed using 20% piperidine in dimethylformamide (DMF) Cycles of deprotection, washings, double couplings, and washings were repeated until the desired chain ... Chapter 156 creating a DNA delivery and tissue engineering dual system Micro-porous PLGA foams engineered by supercritical carbon dioxide foaming technique with large, controlled...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 8
... PLGA/chitosan and PLGA/Lysine composites is in form of naked DNA and its transfection efficiency is extremely low, which would hinder their applications in biomedical engineering In future investigations, ... sustained expression of DNA, which is favorable for DNA delivery and tissue engineering applications In conclusion, this research has shown potential applications of electrospinning and supercritical ... drugs, proteins, DNA or combinations of them) in the future Bone regeneration is a complicated process involving many growth factors and different growth factors have diverse roles in different...
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Direct observations of nickel silicide formation on (100) si and si0 75ge0 25 substrates using in situ transmission electron microscopy
... and indexing of SAED pattern in Figure 5.1 (b) 121 A.3 Indexing of SAED pattern in Figure 5.2 (b) 124 A.4 Indexing of SAED pattern in Figure 5.4 (a) 125 A.5 Indexing of SAED pattern in ... for in- situ deposition of the nickel film using electron beam evaporation followed by the in- situ annealing using direct resistive heating Finally the data acquisition tools, the Gatan Imaging ... mainly in obtaining low sheet resistance on narrow gates with conventional processing18,19,20 The increase in sheet resistance with decreasing line width can be attributed to incomplete transformation...
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Báo cáo hóa học: " In situ Control of Si/Ge Growth on Stripe-Patterned Substrates Using Reflection High-Energy Electron " potx
... dots In the present work, we report on in situ control of Si and Ge growth on stripe-patterned Si (001) substrates using reflection high-energy electron diffraction (RHEED) and scanning tunneling ... Continuation of buffer growth, results in the appearance of faint additional diffraction streaks inclined by 25° to the surface normal, as indicated by the dashed lines in the RHEED pattern of Fig 1c ... sidewall inclination close to 9° as revealed by the corresponding STM image and surface orientation map shown in Fig 2b This transformation to mainly {119} oriented sidewalls is accompanied by a shrinking...
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Báo cáo y học: "Elimination of rheumatoid synovium in situ using a Fas ligand ''''gene scalpel" pptx
... subsequently using an avidin-peroxidase and diaminobenzidine color detection system combined with a hematoxylin counter-stain Terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling ... significant increase in FasL protein expression in RA synoviocytes appears three days after Ad-FasL injection (original magnification 400×) (di) Apoptotic cells in RA synovium were examined using the ... fluorescein in situ apoptosis detection kit (Intergen) The red propidium iodide counter-staining indicates the nucleus of all synovial cells in RA synovium The green fluorescein stains the fragmented...
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A study on manganese catalyzed epoxidation of styrene using online raman and in situ FTIR monitoring techniques
... out kinetic studies using online Raman, in- situ FTIR, 13C NMR and UV-Vis spectroscopy coupled with application of Band Target Entropy Minimization (BTEM) chemometric method The active epoxidizing ... how interesting and fulfilling the actual experience can be Research can be utterly tedious and involves unassuming patience cum perseverance working towards making scientific contribution in ... EPOXIDATION OF STYRENE USING ONLINE RAMAN AND IN- SITU FTIR MONITORING TECHNIQUES QUAH CHEE WEE (B.Eng.(Hons.), NUS) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CHEMICAL...
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Detection of low grade mosaicism in turner syndrome using fluorescence in situ hybridization
... these genes escape inactivation in females to maintain a balance with the situation in males In addition, there are other genes on the X chromosome that are not regulated by X inactivation whose ... the inability of these abnormal chromosomes to undergo X inactivation (Atkins et al, 1966; 13 Van Duke et al 1992) Recently, some cases in which the ring is small and does not contain the X-inactivation ... show a surprising similarity in all the presenting symptoms To explain this, a hypothesis was proposed that in Xq deleted patients the Xinactivation spreads to tip of Xp, thus inactivating the normally...
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In situ catalytic conversion of tar using rice husk char/ash supported nickel–iron catalysts for biomass pyrolytic gasification combined with the mixing-simulation in fluidized-bed gasifier
... article in press as: Shen Y et al In situ catalytic conversion of tar using rice husk char/ash supported nickel–iron catalysts for biomass pyrolytic gasification combined with the mixing-simulation in ... article in press as: Shen Y et al In situ catalytic conversion of tar using rice husk char/ash supported nickel–iron catalysts for biomass pyrolytic gasification combined with the mixing-simulation in ... (mass inventory in an FB), boundary conditions, just like fuel feed points and feed rate, freeboard size, Please cite this article in press as: Shen Y et al In situ catalytic conversion of tar using...
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