pluripotency capacity for in vitro differentiation of undifferentiated embryonic stem cells pdf
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using differentiation- related ... Embryonic stem cells have a unique bivalent chromatin structure which silences developmental genes in ES cells while keeping them poised for activation, thus providing a mechanism for maintaining...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this ... marks to the b-globin gene locus during erythroid differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima ... presence of residual undifferentiated cells We next analyzed the interaction of RNA Pol II and TATA binding protein (TBP) as well as the appearance of modified histones within the globin locus during...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression during ... Biosystems, Warrington, UK) PCR was performed using the Invitrogen Platinum Taq (Invitrogen) method, in accordance with the manufacturer's instructions The PCR primers were designed on the basis of ... cells/ cm2, trypsinised and stored in Recovery Cell Culture Freezing Medium in liquid nitrogen Before osteogenic differentiation, 6,000 cells/ cm2 of human MSCs were seeded in BM in each well of...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... importance of Activin/ Nodal signaling in the maintenance of hESC pluripotency has been re-iterated in recent studies detailing the derivation and maintenance of induced pluripotent stem (iPS) cells ... role for the Notch pathway in differentiation of this multipotent lineage Activation of Notch signaling in combination with inhibition of Wnt signaling was shown to be responsible in part for ... clusters of cells called Islets of Langerhans which perform its endocrine functions Acinar cells secrete digestive enzymes like trypsin and chymotrypsin into the small intestine Islets of Langerhans...
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Báo cáo khoa học: " Investigation of tumor hypoxia using a twoenzyme system for in vitro generation of oxygen deficiency" pot
... out for investigation of hypoxia induced radioresistance The results of our study indicate that the enzymatic GOX/CAT system is an attractive alternative technique for in vitro investigation of ... rapidly induce in vitro hypoxia The GOX/CAT system has been employed in the past in various studies In particular, Baumann et al have applied the enzymatic system for investigation of the effects of ... rapidly induced within 15 and maintained over 24 h [10] Since oxygen transport studies using hypoxia chambers have revealed time periods of more than h for equilibration of pO2 between the medium inside...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... MO, USA) for 10 minutes for chondroitin-4-sulfate (CS4) detection (5 U/mL in distilled water), or with 0.25% trypsin containing mM EDTA for 15 minutes at 37°C for COL X detection, before sections ... double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, fixed overnight in PBS-buffered 4% paraformaldehyde, ... or remained uninfected For comparison, an additional set of untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 ... mesenchymal stem cells from marrow Tissue Eng (4):415-428 129 Beresford JN, Graves SE, Smoothy CA 1993;Formation of mineralized nodules by bone derived cells in vitro: a model of bone formation? Am ... decorin and fibromodulin with transforming growth factor beta Biochem J :527534 24 Iozzo RV 1999;The biology of the small leucine-rich proteoglycans Functional network of interactive proteins...
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Development of a bioreactor for in vitro engineering of soft tissues
... DEVELOPMENT OF A BIOREACTOR FOR IN- VITRO ENGINEERING OF SOFT TISSUES KYAW MOE (B.Eng (Hons.), YTU, Yangon) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF MECHANICAL ENGINEERING ... in designing a bioreactor for the study of the effect of mechanical straining parameters on cellular morphology, to provide a better understanding of condition for the in- vitro growth of engineering ... initial injury while never regaining the properties of the normal tendon/ligament, thus demonstrating the need for tissue engineering approaches During the course of healing, the level of pain decreases...
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investigation of effective media for in vitro propagation of stevia rebaudiana bertoni
... people In this research, in vitro propagation of stevia, was carried out in experiments (callusing, shooting, rooting, and acclimatization) The results supported that MS medium (including vitamins) ... not maintain the callus growth since all explants continued to turn brown and died out The maintenance of callus plays an important role in in vitro propagation through callusing Good maintenance ... is increasing in recent years accompanying with the significant rising rate of mentioned diseases Consequently, large-scale production of this valuable herb is expanded in many provinces of Vietnam...
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In vitro differentiation of stem cells towards islet like cells
... suggested that insulin-producing cells derived from stem cells could be a potential source for cellular therapy in diabetes However, most of the stem cells- derived insulin producing cells obtained from ... issues of embryonic stem cells also restricted its use in stem cells research 1.4.3 Induced Pluripotent Stem Cells (iPS) cells In 2006, induced pluripotent stem cell was first described by Shinya ... reports on differentiation of amnion-derived stem cells into insulin producing cells Wei et al demonstrated the expression of insulin at transcript level after culturing the amnion-derived stem cells...
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Báo cáo khoa học: "Cold storage of in vitro cultures of wild chestnut and oak" pdf
... INTRODUCTION Collections of seeds and clonal material are traditional ways of storing genetic resources Use of cold stored (0-10°C) in vitro cultures is a complementary method of maintaining ... storage offers a potential means of reducing costs of micropropagation and affords an alternative method for conserving genetic resources of forest trees Of the agents named as factors that may influence ... storage of in vitro apple shoots HortScience 14, 514 Marino G, Rosati P, Sagrati F (1985) Storage of in vitro Meier-Dinkel A (1990) Kühllagerung von Gewebekulturen Mitt Bundesforschungsanst Forst...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... CCCTC-binding factor, insulator associated protein; ESC, embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... upregulate expression of PD-L1, iNOS, and COX-2 in MSCs, making these molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation ... anti-CD3-induced T-cell proliferation in vitro by a mechanism involving interferongamma, inducible nitric oxide, and cyclo-oxigenase-2 To investigate the immunosuppressive potential of MSCs in vitro, ...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... labeling includes tetramethylrhodamine isothiocyanate (TRITC)-conjugated phalloidin (staining red F-actin) and DAPI (staining blue nuclei) The staining showed the formation of actin cytoskeleton in ... also induced the formation of bone cavities (BC) containing bone minerals (BM, stained black), Col matrix (COL, stained blue) and bone-lining cells (BLC, stained dark blue) on the surface of new-bone ... incorporation of Dex an osteoinductive drug into polymeric nanofibers during electrospinning process 1) Incorporation of Type I Col into nanofibrous scaffolds can improve nanoHA mineralization on the nanofibers...
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Báo cáo khoa học: The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells pdf
... amount of glycoprotein in cells As shown in Fig 5E, although the total amount of protein in cells decreased to 87% after 48 h of incubation, the amount of glycoprotein decreased more The results of ... lipofectamine solution, dissolving lL of Lipofectamine 2000 in Opti-MEM, and transfected into subconfluent 293A cells in mL of Opti-MEM in six-well plates The 293A cells were then cultured for ... A1-treated PMInull cells We thus performed mannose titration experiments in swainsonine-treated and bafilomycin A1-treated PMI-null cells Cells were treated with 10 lm swainsonine or 100 nm bafilomycin A1...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells (mean ± S.D.; ** indicates ... μg/mL) and selected for infected cells for 48 hours Results FN and LAM enhance differentiation of MSCs into insulin producing cells To examine the effects of ECM, FN and LAM on IPC differentiation ... widespread interest in finding alternative sources of b -cells for tissue replacement strategies in diabetes MSCs have been used for cell-based therapy in regenerative medicine and tissue engineering In...
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báo cáo khoa học: " MicroRNAs involved in neoplastic transformation of liver cancer stem cells" pot
... and differentiation of normal stem cells strongly suggest that miRNA may be involved in the transformation of normal stem cells into cancer stem cells Therefore, screening for differences in miRNA ... aberrant differentiation According to the hierarchical model of cancer, the origin of the cancer stem cells may be long-lived somatic stem cells Therefore, markers of “normal” stem cells are being ... and progenitor cells, but is absent in breast cancer stem cells The reintroduction of let-7 into these cells causes differentiation and reduction of proliferation and tumor-forming ability It...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... (b) Immunostaining with bone specific ALP (arrow showing the cells positive for ALP stain) (d) Immunostaining with osteonectin (arrow showing cells positive for osteonectin staining) 50 ... time-point: (a) H&E staining showing newly formed tissue within the scaffold (arrow showing scaffold remains) (b) von Kossa staining and counterstaining with nuclear fast red showing deposition of ... hFOB H & E staining (d) hFOB von Kossa staining Scaffold (e) hFOB H & E staining Figure At 2-week time point: H&E staining and von Kossa staining of sections showing scaffold and cells (e) hFOB...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... pluripotency in human ES cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It ... achievement of iPS cells, by overexpressing four genes containing LIN28, reinforced the notion that LIN28 is a key regulator of pluripotency in human ES cells (Yu et al., 2007) 1.4.1 The interaction of ... performed on the following day using Lipofectamine RNAiMAX (Invitrogen) at a ratio of 60 pmol of siRNA pool : µl of transfection reagent Page 25 siRNA and Lipofectamine were diluted in 50 µl of...
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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... days of humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse Embryonic Stem Cells ... subsequent engineering of short, hairpin vectors under the control of Tet-R, have the advantage of being easily created for the specific gene of interest We selected the Invitrogen BlockIT Tet-inducible ... Mouse embryonic stem cells (mESC) are uniquely capable of differentiating into all somatic cell types in the body, a property known as pluripotency In addition, mESC are known to grow indefinitely...
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