... directly involved inthe formation of retinal NV inthe later stages ofthe OIR model In this study, we investigated the temporal and spatial distribution of microglial cells during the different ... pictures and drafted the manuscript GM participated inthe design ofthe study and performed the statistical analysis HTA conceived ofthe study, and participated inits design and coordination and ... the border ofthe inner nuclear layer andthe outer plexiform layer (Figure 1B and 2E) Microglial cell density was always higher inthe superficial than inthe deep retinal layer (Figure 3) The...
... design ofthe study and analysis and interpretation of data HB conceived ofthe study, participated inits design and coordination and helped to draft the manuscript All authors read and approved the ... copies) and was maintained until the end ofthe study at day 10 Similar to cells infected with the MOI of × 10-1, no increase in negative strand RNA was observed (Figure 4D) Beta actin levels inthe ... carried out the molecular and cellular studies and drafted the manuscript DR carried out thein vivo and cellular assays and analysis and interpretation of data, EJM, MW and CL participated inthe design...
... focused on a small number of cytokines within the same sample It is not clear which cytokines are key in promoting and maintaining the inflammatory environment inthe AD brain Furthermore, it is unclear ... from transgenic mousemodelsof Alzheimer's disease, and to further relate this increase to the level of Aβ present inthe brain Another purpose of our study was to determine whether inflammatory ... ofthe pathological features seen inthe AD patient brain and show an increased production of inflammatory markers such as COX-2, PGE2 and also increased levels ofthe pro-inflammatory cytokines...
... was removed within days in every run Assuming the zero-order kinetics, the average cadmium removal rates within the days calculated from the linear approximations ofthe plots in Fig were estimated ... like inthe case of cadmium was not observed Furthermore, the particular effect ofthe cadmium addition on the appearance ofthe plant body was not observed during days in runs to Assimilation of ... cadmium, and is synthesized from glutathione (GSH) Inouhe et al (1994) reported that the cadmium binding complex containing PCs has an important role for the cadmium-tolerance of seedlings of some...
... for choosing the topic, the aims ofthe study, the methodology, the scope, andthe design ofthe study Part B, the main part ofthe thesis, consists of three chapters Chapter 1, entitled Theoretical ... giving, and (ii) demanding” This means there are two roles in exchange: giving and demanding He goes on to analyze the nature ofthe commodity being exchange into (a) goods and service and (b) information ... features ofthe text andthe intention ofthe writer In this thesis, the text chosen is the short story The Law of Life” by the famous American writer, Jack London Aims ofthe study The study...
... partitioning ofthe protein into an aggregated state The effect of copper binding on the secondary structure was determined using CD inthe far UV To prepare proteins without Cu2+, part of each protein ... copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable of domain swapping and forms ... understanding what causes the loss of appropriate metal binding might be crucial for the understanding ofthe role of amyloidogenic proteins in a number of neurodegenerative disorders Copper binds...
... concerning the kinetics and properties ofthe enzyme More strikingly, we show a novel role ofthe PPase enzyme inthe development and molting process of A suum larvae in vitro constructed in UniZap ... (CostarÒ) The cultures were incubated at 37 °C in a humidified 5% CO2 incubator inthe absence (control) and presence of increasing concentrations of inhibitors for 10 days, andthe number of molting ... immunohistochemical staining), and that the inhibition of PPasecatalyzed PPi hydrolysis by these inhibitors, is likely to prevent the synthesis ofthe new cuticle from the hypodermis and/ or ecdysis ofthe old...
... advantage ofthe trypsin-sensitive region situated inthe linker domain of Abcb1a Following trapping inthe absence or presence of Vi and mild-trypsin treatment, the trypsin degradation products ofthe ... inthe glutamine (length ofthe side chain maintained) and asparagine (shorter side chain) mutants, then trapping inthe absence of Vi now occurs [39] (Fig 3); this is also the case when the ... Another notable feature ofthe single-site IC mutants (including the glutamine substitutions) [39] is the fact that, inthe absence of Vi (± drugs) labeling ofthe enzymes with the mutation in...
... clarified in detail and could play a key role inthe detoxification processes In this study we examined the role of Bcp2 in order to increase the knowledge ofthe enzymatic activity involved inthe ... Furthermore, we report the cloning, the expression andthe characterization ofthe recombinant protein rBcp2 in order to shed light on its role inthe detoxification process and on its catalytic mechanism ... 2-Cys Prxs, the largest Prx class, is their second redox-active cysteine, the resolving cysteine (Cys-SR) In typical 2-Cys, Sp at the N terminus and SR at the C terminus ofthe protein belong to...
... how long the enamine remains inthe active site andits rate of attack on the aldimine bond, and can be modified by slight differences inthe position ofthe residues [41] As covalent binding was ... becoming the major form detected inthe spectra Summarizing, data shown in Fig reinforce our previous findings on the tautomeric forms ofthe internal aldimine inthe free holoenzyme [19] andthe ... times were 2–5 The conformational changes induced in HDC by histidine and histidine-analogs involve alterations inthe environment ofthe aromatic amino acid residues ofthe protein Fig Thermal denaturation...
... cause, given that there is an increased use of Q-queries in spite ofthe seemingly inferior search results, there is a strong need for the search engines to improve their handling of question-queries ... clustering but on understanding the expression of intent Method We address the main thesis ofthe work by retrospectively studying queries issued to a search engine over the course of 12 consecutive ... context and an improved understanding of question-queries by search engines Related work There has been some work on studying and exploiting linguistic structure in web queries Spink and Ozmultu...
... mislocalization of all three enzymes in these cells clearly shows the involvement of PEX14 inthe targeting of PTS-1, PTS-2 and I-PTS proteins to the glycosome To further analyse the effect ofthe destruction ... N-terminal domain ofthe polypeptide of each of these organisms is followed by a stretch of hydrophobic amino acids that is presumably involved inthe protein’s anchoring to the membrane Also in ... was located inthe band This may be due to diffusion of some complexed protein as the band migrates inthe native gel Band contains the excess of TbPEX14-N,H In a second experiment, the same two...
... degree of insight into the workings ofthe four elements, and also to the many friends and students who have encouraged my writing and teaching Finally, we appreciate the permission ofthe following ... It has nothing to say concerning the why ofthe universe, and everything dealing with the understanding andthe significance of individual human values and goals is outside its domain astrology's ... the domain of psychologT to the laboratory study of animals and to the overt behavior patterns of human beings is inconsistent with the definition ofthe supposedobject of study: the psyche itself,...
... Language of Energy Key Concepts and Deftnitions A key to the understanding of all astrology is within the reach of anyone who truly understands the meaning ofthe following definitions: The PLANETS indicate ... according to the elements have a much better chance of constructively meeting the challengesoftheir stressful aspects Since most ofthe principles involved in comparing the interaction and combination ... considerations, or intuitions ofthe other signs They are attuned to the world of "forms" which the sensesand practical mind regard as real, and their innate understanding of how the material world...
... either at a very low level or were below the level of detection ofthe assay inthe non-infected control group, andthe objective ofthe study was to evaluate the effect of motavizumab inthe inflammatory ... time ofthe administration ofthe anti-RSV antibody had no effect on the cytokine profile or other clinical and inflammatory parameters evaluated, therefore those controls were not included inthe ... markers of disease severity correlate with similar changes in cytokine profiles These studies will provide further understanding ofthe pathogenesis of RSV infection and should help identifying new...
... was applied to the nares of mice anesthetized by intraperitoneal (i.p.) injection of 0.2 ml ketamine-xylazine (70/7 mg/kg); this results inthe aspiration ofthe vaccine into upper and lower airways ... with the few mice used in these experiments Taken together, the results indicated that combined vaccination with M2e-MAP and infectious virus may improve the induction of HeLa-M2-reactive Abs and ... against viral structures of low or no variability, and thereby provided a constant level of long lasting resistance against IAV infection, independent ofthe glycoprotein makeup of circulating...
... contributed inthe analysis and interpretation ofthe data, participated inthe idea and design ofthemodels proposed andinthe drafting and revision ofthe manuscript IDM collected the data resulting ... (figure 1) In anticipation ofthe difficulties expected in manipulating the left end hairpin and to increase the chances of obtaining the desired construct two copies ofthe left end termini were ... essential cofactor for NS1 inthe replication initiation process allowing efficient and specific nicking ofthe 3' minimal origin and leaving NS1 covalently attached to the 5' end ofthe DNA at the...
... important role inthe secondary activation of microglia, inthe progression ofthe inflammatory response, andinthe progressive loss ofthe dopaminergic neurons in MPTPinduced PD Therefore, COX-2 ... Boehringer-Mannheim, Indianapolis, IN) or βactin (Sigma, St Louis, MO) antibodies for detection ofthe level of dopaminergic neuronal terminals, and for normalization ofthe loading protein The signal ... fill inthe gap within the literature on the effects of COX-2 inhibition in protecting SNpc dopaminergic neurons from MPTP-induced neurotoxicity We inhibited COX-2 using both pharmacological and...
... either at a very low level or were below the level of detection ofthe assay inthe non-infected control group, andthe objective ofthe study was to evaluate the effect of motavizumab inthe inflammatory ... time ofthe administration ofthe anti-RSV antibody had no effect on the cytokine profile or other clinical and inflammatory parameters evaluated, therefore those controls were not included inthe ... markers of disease severity correlate with similar changes in cytokine profiles These studies will provide further understanding ofthe pathogenesis of RSV infection and should help identifying new...