... Environment ofthe Commission on the strategic direction ofthe CAFE programme The approaches to answering the questions were rather complex The procedure for preparing the report Health aspects of air ... The designations employed and the presentation ofthe material in this publication not imply the expression of any opinion whatsoever on the part ofthe World Health Organization concerning the ... However, the results ofthe systematic review confirmed the existence of severe effects of PM and ozone on human health even at concentrations at the lower end ofthe current ranges Thus the objective...
... hevamine, the oxygen atom ofthe N-acetyl group ofthe )1 sugar is positioned close to the C1 atom ofthe )1 sugar, and is hydrogen bonded to Tyr183 Asp125 makes a hydrogen bond to the nitrogen atom of ... substrate binding, as the Km value ofthe Tyr183Phe mutant hardly differs from that ofthe wild-type enzyme (Table 4), the Kcat value of this mutant has dropped by 80% (Table 4) Fromthe structural ... away fromthe C1 atom ofthe )1 sugar (Fig 3) Apparently, as witnessed by the structure ofthe Asp125Ala/Glu127Ala mutant, the interaction with Tyr183 alone is not strong enough to keep the N-acetyl...
... when the first cold storm ofthe world sweeps over it, if the warm sunlight of love fromthe eyes of mother and father did not shine upon him like the soft reflection of divine light and love? The ... fall, so that only the tops ofthe trees and the peaks ofthe church steeples and the rising smoke fromthe houses defined the limits which separated the reality ofthe world from its reflection ... suggestions; to Prof Feuling, the eminent philologist, ofthe University of Wisconsin, for his literal version ofthe extracts fromthe "Deutsche Theologie," which preserve the quaintness ofthe original,...
... necessity The most powerful of these Rajput chiefs was the Prince of Mewâr who had particularly attracted the attention ofthe Emperor by his support ofthe rebels The control of Mewâr rested upon the ... places, but declares at the bottom of page 135, "The reign of Akbar is one ofthe most important in the history of India; it is one ofthe most important in the history ofthe world"); Mountstuart ... devote themselves to his service, and, under the leadership of their own princes, proved themselves to be the best and truest soldiers ofthe imperial army, even far from their home in the farthest...
... my hat and out darted one ofthe birds; then I lifted the hat fromthe other One ofthe females then rushed, apparently with notes of joy and congratulation, to one ofthe males, who gave her a ... variety of motion and attitude, fromthe peak ofthe barn or hay-shed As yet, you may have heard only the plaintive, homesick note ofthe bluebird, or the faint trill ofthe song sparrow; and the ... carry the head as if it were riveted to the body Not so the oven-bird, or the other birds that walk, as the cow-bunting, or the quail, or the crow They move the head forward with the movement of the...
... inhibited the production of TNF-a by itself, i.e in the absence of Iris (data not shown) From these results, we conclude that the interaction site responsible for the anti-inflammatory effects of Iris ... contrast to the effect of rIris on fibrinolysis, an activity lost in the RCL mutant [21] This indepen3242 dence ofthe protease inhibitory activity ofthe anti-inflammatory activity of Iris was further ... ⁄ Pi The effect of these samples on cytokine production by PBMCs activated by LPS was then assessed as described above Prediction of interaction sites The prediction of binding sites from the...
... inhibition ofthe PKC a, b1, b2, c, d, e and f isoform enzyme activity in the presence ofthe PKI55 protein and the derived peptides 5, and 9, all tested at a concentration of lM The data are the mean ... peptides The level of PKC a, b1, b2 and f isoforms was also studied Results Synthesis of peptides derived from PKI55 and their inhibitory effect on rat brain PKC A series of peptides was synthesized ... but acquired the ability to inhibit the e isoform, whereas peptide was only effective on the b1, e and f isoforms Interestingly, the inhibitory action of peptides and on the b1 isoform was found...
... ‘nested PCR’, using the first PCR product as template To make use ofthe BamHI site in the vector and the XbaI site in the EhMGL1 gene, the product ofthe nested PCR was replaced with the corresponding ... Despite the heterogeneity ofthe EhMGL1 preparation used in the previous study [13], the kinetic constants of EhMGL1 in the present study largely agreed with the previous data, except that the relative ... Taken together, these findings suggest that EhMGL1 is responsible for the decomposition and the maintenance ofthe cellular concentrations of Met and Cys, whereas the physiological substrates of EhMGL2...
... complexes, the assembly ensures the transfer of electrons from different sources to oxygen by the respiratory chain complexes and the coupling of proton uptake fromthe matrix compartment [28] The nature ... oxidation ofthe substrate DHO with the quinone cosubstrate was coupled to the reduction ofthe chromogen 2,6-dichloroindophenol The Km of DHO was determined by varying the concentration of DHO ... contact between the ubiquinone fromthe inner membrane and the active site of DHODH [23,27] Although there are some differences in processing and association in the organization ofthe fungal and...
... and the first transmembrane domain (Fig 1B) Protein expression To verify that the constructs produce proteins ofthe expected masses, we attached the V5 epitope to the C terminus ofthe CRFR1 isoforms ... (Fig 2) The molecular mass (including tag) of these isoforms is listed in Table Thus, the mRNA fromthe alternatively spliced CRFR1 forms is translated into final protein products, which are the subject ... alpha isoform were similar for CRF and urocortin (Fig 3, Table 2) None ofthe other isoforms had any effect on cAMP accumulation when transfected alone with the exception of CRFR1h2 (Fig 3) In the...
... to the instructions ofthe kit from Ambion (Austin, TX, USA) The cDNA mix was synthesized from poly(A)+ mRNA using M-MLV reverse transcriptase The cDNA was joined with the adaptor provided by the ... is fromthe a-ScTXs, the second is fromthe b-ScTXs Birtoxin is the shortest The depressant and the long-chain excitatory are in the last two lines The right columns indicate percentage of similarities ... to the a-ScTxs (data not shown) However, due to the uniqueness of its sequence, it branches independently ofthe other a-ScTxs Figure also shows that the core ofthe three disulfide bridges of...
... coil ofthe C-terminal end ofthe protein (C-terminal to helix IV) (not shown) The long distance between these two residues in the predicted tertiary structure would prevent the formation of an ... conserved iron ligands: the three histidines and the first glutamate ⁄ glutamine ofthe motif When using multiple clustalx [9] alignments ofthe MCA0715-encoded protein with members ofthe Hr (Fig 4A) ... distantly to the members of both the eukaryotic Hr and MHr subfamilies (Fig 5) The highest similarity between members ofthe haemerythrin family and McHr was observed with the Hr alpha chain of Lingula...
... as the number of web pages containing the words the and ’and’ matched On the basis of these, we then calculate the probability of a certain qualia element given a certain role on the basis of ... allow to find an ideal cut-off point to increase the precision/recall trade-off ofthe learned structures We have abstracted fromthe issue of finding the appropriate cut-off, leaving this for future ... clue c, the query c(n) is sent to the web search engine and the abstracts ofthe first m documents matching this query are downloaded Then the snippets are processed to find matches ofthe pattern...
... assumes that the thiol group of Cys135 adds to C6 ofthe pyrimidine ring, favoring the exit ofthe aminic group The subsequent addition of a water molecule and the expulsion ofthe active cysteine ... identity and 51% similarity to the TenA protein from B subtilis The 3D structure ofthe monomer is quite similar to that ofthe other members ofthe TenA family of known structure: twelve a-helices, ... biosynthesis Scheme ofthe pathway for the synthesis of thiamin in H pylori, based on the genes coding for enzymes potentially involved in thiamin biosynthesis identified to date The substrate of the...
... sequence identity The length ofthe macrocypin (Mcp1) deduced amino acid sequence was 169 residues, with a molecular mass of 19 193 Da, while the length ofthe representatives ofthe other four groups ... coli, and the NH2-MGFE sequence of rMcp1 favours only partial cleavage The far-UV CD spectra of rMcp1 and rMcp4 confirmed the expectation fromthe sequences that the conformations ofthe inhibitors ... inhibitors of papain, cathepsin L and cathepsin V, with a mean Ki value of 0.5 nm Macrocypin was also an effective inhibitor of papain but exhibited weaker inhibition of cathepsin L and V than the other...
... range from to 8.5, we studied thermal stability of 17b-HSDcl from CD and UV melting curves, from which the temperature of denaturation (Td) and the van’t Hoff enthalpy of denaturation (DH°vH) of ... stability These data thus show that the absence of induction of thermal stability by NADPH binding is the consequence of enthalpic compensation ofthe disturbed intramolecular interactions by the newly ... calculated fromthe far-UV CD spectra using contin software [21] The degree of reversibility ofthe urea- and GuHCl-induced unfolding of 17b-HSDcl was determined by measuring the CD spectrum of 17b-HSDcl...
... pothesis have attempted to include these theories [5-8] The theory of Greaves and that of Kinlen have been discussed in one ofthe chapters in this book One ofthe limitations ofthe theory of ... the peak age of onset of ALL reflects the degree of susceptibility with which a child is born and, on the other hand, the degree of proliferation ofthe cells involved in the development ofthe ... factor, the effect of said exposure will vary according to the stage of development ofthe in‐ dividual or ofthe particular organ [47-52] Some ofthe factors that can influence the toxicity of a...
... ratio of : 5000, appearance of a peptide product of correct mass for cleavage ofthe C-terminal glutamate fromthe b-endorphin peptide was observed after The amount of this product relative to the ... at the 5¢ end, and starts at nucleotide ofthe coding sequence The sequence at the 5¢ end ofthe mRNA was completed by 5¢ RACE, from which two independent clones gave identical sequences at the ... any of these substrates in the absence ofthe recombinant carboxypeptidase Because the activation of mammalian digestive procarboxypeptidases in vivo is known to be caused by cleavage of the...
... is the angle between the projection ofthe tilt ofthe z axis on the xÂ, y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location ofthe rhombic (x, y) axes The ... conservation ofthe magnetic anisotropies of low-spin hemin with His/cyanide ligation The 10 tilt ofthe major magnetic or z-axis is shown to be consistent with the % 10 tilt ofthe Fe-CN vector fromthe ... important portions ofthe protein that we have characterized above Thus parts ofthe FG corner move away fromthe heme and the B-helix moves closer to the heme in the WT metHb than in the rWT metHbH2O...
... decline ofthe shares’ price during the first part ofthe year, there had not been any sign of depositors’ massive withdrawals before September This is a further proof that despite the existence of ... as a lender of last resort The first section will relate the story ofthe Northern Rock bank In light of those facts, the second section will discuss the impact ofthe changes introduced by internet ... branches formed, the length ofthe queue being aggravated by the break down ofthe bank website and its call center The reason for the initial panic has to be found in the type of deposit insurance...