new insights into the epithelial sodium channel using directed mutagenesis
Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx
... a hinge region Furthermore, the nature and location of the catalytic residues as well as the enzymatic mechanism (they are cyclizing RNases) are also conserved [16–18] For these reasons, ribotoxins ... in the active site The results obtained will be discussed and compared with those reported for other members of the fungal extracellular RNase family Results Assignment The 1H assignments for the ... from the idealized covalent geometry, and most of the backbone torsion angles for amino acid residues lie within the allowed regions in the Ramachandran plot The statistics characterizing the...
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Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot
... H.-S Chen et al A B the corresponding D ion Third, the 0,4A ion would include the 6-arm substituents, but not the extra GlcNAc residue, if the latter bisects the b-Man residue at the C4 position ... First, the D ion formed at the bisected 3,4,6-linked b-Man residue carried the extra bisecting GlcNAc residue together with the 6-arm substituents Second, a characteristic loss of both the bisecting ... proteins were sequenced Of these, 10 clones were found to encode the LC2 and LC1 subunits Others were found to encode other variants of the C-lectin-like venom proteins The amino acid sequences...
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Báo cáo khoa học: New insights into the P-glycoprotein-mediated effluxes of rhodamines doc
... also the measurement of the rate of passive diffusion of the dye through the plasma membrane This cannot be done by the simple measurement of the increase of the fluorescence signal (Fcyto) of the ... ratio of the drug bound to the drug free in the cytosol, which subsequently allows the determination of the real number of molecules that penetrate per second into one cell and therefore the true ... reaching the steady state, rhodamine continuously enters into the cells According to Eqn (6), during dt, the increase in the intracellular rhodamin concentration is (1 + b)ÆdCi and the increase of the...
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Click Here: The State of Online Advertising - New insights into the beliefs of consumers and professional marketers ppt
... Visit the product's website 11% Visit the product's social media page Other 6% Like' the product 5% 'Comment' on the product 4% Hide that person from your social media newsfeed 2% Recommend the ... Consumers pay far less attention to ads than they the speed limit Attention Consumers 74% Posted Speed Limits 69% Spouse or Significant Other 63% Weather Report 54% Dentist Boss 52% Parents 51% ... favorite TV show are the two most preferred places to look at an ad Majority of respondents use social media; over half have liked on behalf of their favorite brands, but also wish there was a dislike...
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Báo cáo hóa học: " New Insights into the RLS Algorithm" pdf
... three factors: the exponential window, the level of noise at the system output, and the condition number The closer the exponential window is to one, the better the misalignment is, but the tracking ... added to the input x(n) with different SNRs The range of the input SNR is −10 dB to 50 dB Therefore, with an input SNR equal to −10 dB (the white noise dominates the speech), we can expect the condition ... how the misalignment will degrade by increasing the condition number of the input signal For example, by increasing the condition number from to 10, the misalignment will degrade by 10 dB; the...
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Báo cáo sinh học: "Making the jump: new insights into the mechanism of trans-translation" pdf
... systematic site -directed mutagenesis study in an attempt to establish the contribution of the nucleotide residues that precede the resume codon to the correct positioning of the MLD Identifying ... responsible for engaging the resume triplet and tmRNA in the attachment and synthesis of the tag peptide They speculate that A86 might bind to the SmpB that is part of the transfer-messenger RNA ... resistance assay They found that eight of the 18 codons that were prohibited according to the -1 hypothesis [12] were in fact fully functional, and other mutant tmRNAs that were predicted by the -1 triplet...
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báo cáo khoa học: " Construction of a potato consensus map and QTL meta-analysis offer new insights into the genetic architecture of late blight resistance and plant maturity traits" pps
... is the number of hypothetic meta-QTLs of a model) “Delta” is the rescaled value of the criterion, it is the difference between the value of the given model and the value of the best model The ... up the hypothesis that the same genes are present in the same order in the genome across species, and that the genetic variation, if any, would take place at the allele level The fact that the ... markers of the QTLs on their original maps and the positions of these markers on the consensus map The MetaQTL software first took into account the confidence interval reported in the study if...
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Báo cáo y học: "New insights into the pathogenesis of glucocorticoid-induced avascular necrosis: microarray analysis of gene expression in a rat model" potx
... rodent diet The weight of the rats was followed Page of 12 before and after the implant of a prednisone pellet for the first three consecutive weeks and then every month until the end of the experiment ... heterogeneity of the phenotype expression between endothelial cells in the body, a local endothelial cell dys- function can occur where the femoral head endothelial cells react differently to the ANFH ... the Femoral Head The other authors declare that they have no competing interests Authors' contributions All authors participated in the study MAK made a major contribution to the writing of the...
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Báo cáo khoa học: New insights into Fragile X syndrome Relating genotype to phenotype at the molecular level docx
... perturbations in the vicinity of the introduced mutation, with the overall fold conserved The data presented here represent the first biophysical characterizations of the KH domains of dFXRP The structure ... FMRP function as the primary cause of the syndrome In spite of its key importance, the effect of the I304N mutation on the structure and function of FMRP is still unclear, and there have been ... interaction in the cocrystal structure of the KH3 domain of NOVA bound to an in vitro selected RNA [17,18] Yet another hypothesis is that the I304N mutation causes FMRP to associate into ribonuclear...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... Finally, by comparing ApeSOD with the SOD from the thermophilic bacterium Thermus thermophilus, we evaluate the relationship between electrostatic interaction and the protein’s stability in an organic ... This was attributed to the Mn or Fe ions incorporated into the enzyme as it accumulated in the E coli cells Indeed, the activity of the apo-enzyme was significantly lower than the metal-containing ... of ApeSOD The line reflects the best fit of the data and indicates that the apparent molecular mass is 96 265 Da The deviation between empirical data and the fitted line is plotted in the upper...
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... it takes the position of the adenine ring in the ATP-binding site, as shown in Fig The oxygen atoms of the pantoate are spatially coincident with the nitrogen atoms of the adenine ring The O1, ... in the A-chain These residues 704 form the ‘gate loop’, which is thought to play a significant role in channeling the substrate into the active site in the M tuberculosis protein The deviations ... in the presence of ATP, the His34 backbone HN chemical shift reverted back to the resonance position observed for the free form of the protein The observed changes in resonance positions in the...
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Tài liệu NEW INSIGHTS INTO TOXICITY AND DRUG TESTING pptx
... that in the U.S a new case of Alzheimer's develops every 68 seconds [7] Using these figures, more than 460 000 new cases of Alzheimer's will develop each year the approval of an effective new drug ... in the surrounding culture medium Maybe these markers will fluctuate in a way 19 20 New Insights into Toxicity and Drug Testing similar to what would occur in the in vivo setting and it may therefore ... contribution to the knowledge of microcystins toxicokinetics in the kidney and, consequently, an important stimulus to the investigation of microcystins toxicity in the kidneys 31 32 New Insights into Toxicity...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... maintained together in either the test set or the working set Although ˚ the data could be processed up to 2.5 A, there were some practical problems in using the full dataset in the refinement – the twin ... proton from the catalytic Cys present at the N-terminus of the helix to the His of the dyad [19,50,51] The first stage of catalysis is mediated by the highly active thiolate ion of the Cys Biochemical ... ervatamin-B) at the bottom of the subsite is conserved in ervatamins Compared to a Ser in the equivalent position of papain, the role of Leu here is to restrict the size and depth of the S2 subsite The S2...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... [19], and poppy [20,21] In the present study, the first insights into the secondary and tertiary structure of PLDa2 from cabbage are given The surface structure of the enzyme was investigated ... illustrates the quality of the experimental data (Fig 2B) Reconstruction of the overall shape of the PLDa2 from the X-ray scattering data was achieved by the ab initio modeling program dammin [24] The ... binding, or adjusting the charge distribution at the active site Unfortunately, the number of calcium ions bound to the enzyme cannot be deduced from these data The binding event with the dissociation...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf
... protein to insert into and be stable in the inner mitochondrial membrane or lack of detection of the truncated protein by the antibodies In the W303–1B q° strain the amounts of the other two catalytic ... single deletion mutants The values represent the percentages of the amounts of the individual subunits present in the yeast mutant strains with respect to the amounts present in the wild-type strain ... from the W303–1B q° strain Likewise, no cytochrome b protein was detectable in the CKL57 strain We not know whether the lack of immunoreactivity in the latter strain was due to the inability of the...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... pH 7.5 Site -directed mutagenesis The His120Asn, His145Asn and Asn149Asp mutant forms of human arginase II were obtained by a two-step PCR [40], using the QuickChange site -directed mutagenesis ... to convert the enzymes to their fully active state In contrast, the arginase activity of the Asn149Asp variant was practically undetectable, both before and after the incubation with the manganese ... being involved in the stabilization of the transition state [27], but not in the stabilization of the substrate in the Michaelis–Menten complex [24] Also in agreement with this, the Km value was...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... of the manganese ions This, together with the fact that the positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the ... insertions or deletions in the agmatinase sequence were modelled using the DGLOOP set of options in WHATIF; the whole loops and the two connecting amino acids at the beginning and the end, were mutated ... indicating that the environment of tryptophan residues is essentially conserved in the mutant enzyme On the other hand, the absence of major differences in the CD spectra of the wild-type and...
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Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot
... that the former is likely to be in the Cterminal half and the latter in the N-terminal half of the protein [29,48±51] Sequence-based structure prediction methods suggest that the caseins are of the ... corresponds to the 412-amino-acid isoform of human brain tau At neutral pH, the tau samples showed aggregation in the laser beam, with the aggregates falling to the bottom of the cell, so that the concentration ... strand (rather than b sheet from the appearance of the amide I ROA, see below) [18] The negative intensity in a similar region of the ROA spectrum of b-casein may have a similar origin The two...
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Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx
... above, the crystal structures of the three complexes not differ significantly in their overall structure In order to determine whether the same is 2634 T Werther et al true for the structure of the ... atom pairs) The locations of the cofactor ThDP and the activator ADP are clearly defined in the electron density map In contrast, electron density of the S-acetyl pantetheine moiety of the inhibitor ... of the adenine ring and the c-carboxyl group of the side chain of residue D302, the d and x nitrogen atoms of the guanodino group of R158 interact with the hydroxyl groups of ribose, and the...
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Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx
... if the ribityl chain in the other structures The distance between the pyrimidine ring and the phosphate atom in the phosphate moiety is 6.9 A The location of this group is the same as in the ... isotherms for the inhibitors (F) The lled circles in the binding isotherms represent the experimental values of the heat change at each injection; the continuous lines represent the results of the ... that the shift of the phosphate moiety from the aromatic purinetrione system to the periphery of the active site is restricted by the position of Arg128 from one side and the conformation of the...
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