... Ó FEBS 2004 Dynamic regulation of HO-1 expression in RPE (Eur J Biochem 271) 3077 visible light [22] and in cultured ... (Stratagene, La Jolla, CA, USA), then cloned into pCR-bluntII-TOPO (Invitrogen, Carlsbad, CA, USA), and named pCR-hHO1 RT-PCR was performed for preparation of the human TGF-b1 cDNA First strand cDNA was ... solution, amplified fragment was cloned into pGEM-Teasy vector (Promega, Madison, WI, USA), and named pGEM-hTGFb1 SP6 RNA polymerase was used for transcription of RNA probe from pCR-hHO1 Renilla...
... two of the six hybridomas was inhibited by addition of the neo-epitope These two antibodies were named Monoclonal and Monoclonal Frozen sections were prepared as previously described [33] After...
... Gelkop S, Shin NY, Hanks SK, Pawson T & Mayer BJ (2006) Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B Proc Natl Acad Sci USA ... 26 Hall A & Nobes CD (2000) Rho GTPases: molecular switches that control the organization and dynamics of the actin cytoskeleton Philos Trans R Soc Lond B Biol Sci 355, 965–970 27 Nakamura N,...
... vitro into biological membranes, where it supports the same alterations as native lipids [43–46] Namely, peroxidation of this probe induces a decrease of its emission fluorescence Fig Effect of Bax ... presence of C18:2 as compared to C18:1 and control The effects of known inhibitors of lipid oxidation, namely a-tocopherol and resveratrol were also assayed: both compounds significantly decreased kinetics ... cytochrome c relocalization [25], which is likely to interfere with the response of these probes, namely H2-DCFDA, to ROS [48] The experiments were therefore reproduced in yeast cells expressing...
... Notes Identification of single frames by a time code is critical for the accurate measurement of dynamic events The most accurate time code has been established by the Society of Motion Pictures...
... in tenosynovitis [12] We therefore examined the effects of hypoxia on proangiogenic molecules – namely, VEGF, PlGF and VEGF/PlGF heterodimer VEGF was used as the control for the present study,...
... [6] The present study found that 1% of TXL induced the cleavage of caspase-3 to its active form, namely p17 (Figure 4) The fragment, p17 (17 kDa), was accumulated after 24 hours of TXL treatment...
... cells and the obstacles under consideration The dynamics of cell movement very close to the obstacle surface are also influenced by short-range hydrodynamic interactions between the two In the interest ... Nuclear Physics Wiley; 1987 Lamb H: Hydrodynamics Cambridge: Cambridge University Press; 1997 Grima R: Phase transitions and superuniversality in the dynamics of a self-driven particle Phys Rev ... approach involves using Stoke's stream function, ψ, which is a common method for solving hydrodynamic problems [19] The trajectory of a cell corresponds to ψ = k, where the constant k is determined...
... ···········································································28 1.13.3 Live imaging opens doors for studying the dynamics of cell migration in vivo···················································································31 ... protrusions in border cells·································88 3.8 Physiological function of actin dynamics in border cell migration·················91 3.9 Arp2/3 and Dia act in different ways in border ... solution, suggesting that the activity of VASP requires some form of attachment Generally, the net dynamics of actin filament are determined by nucleation, branching, elongating at one hand and severing...
... examining the kinetics of actin polymerization in tubes (by means of spectro-fluorimetry) and the dynamics of actin cytoskeleton in the context of the whole cell (by means of fluorescence microscopy)...
... 3.5.2 Kanamycin treatment: E coli produces hydroxyl radicals in response to kanamycin This is observed in the Kohanski study and by us during our assay development experiments Since, Kanamycin ... ofloxacin isoniazid, ethambutol, and kanamycin All drugs were purchased from Sigma-Aldrich 25 The fluoroquinolones were prepared at a concentration of 0.5mM Kanamycin, isoniazid and ethambutol were ... 3.5 Hydroxyl radicals in response to Isoniazid, Ethambutol and Kanamycin Different concentrations of Isoniazid, Ethambutol and Kanamycin were applied to cell cultures and hydroxyl radicals were...
... was observed in Zn-treated cells [37–39] Intracellular Zn exists as fixed pools of Zn or as more dynamic and labile Zn pools, which are thought to be associated with the regulation of apoptosis by ... of apoptosis with change in intracellular labile Zn (II) using Zinquin [(2-methyl-8-toluenesulphonamido-6-quinolyloxy) acetic acid], a new specific fluorescent probe for Zn (II) Biochem J 296, 403–...
... by a double outline around the name together with the corresponding functionally related symptom of SEB All proteins are indicated by a single outline around the name Symbol ‘X’ indicates inhibition ... chondroitin sulfate proteoglycan versican V0 splice-variant precursor peptide ND, not determined Gene name Sequence ID CTAP-III CTSL HIF-1 GBP-2 IL-17 IL-6 Prot-Vo BC028217 NM-001912 AF050127 M55543 ... supplemented with 20 unitsÆmL)1 penicillin G sodium, 20 lgÆmL)1 streptomycin sulfate, 20 lgÆmL)1 kanamycin sulfate, and minimal essential Immunoblotting and western blotting The cells were lysed...