miniaturization technologies for high throughput biology
Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... can be produced in high yields in vivo are also suitable for efficient production by cell-free synthesis In order to compensate for the increased effort and expense required for the production ... proteins makes it an attractive tool for preliminary studies prior to the production of uniformly [15N ⁄ 13C]labelled samples for in-depth NMR analysis Much information can be gleaned already from ... particularly attractive for high- throughput production of proteins and selective isotope labelling starting from relatively expensive isotope labelled amino acids A straightforward combinatorial...
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bioinformatics for high throughput sequencing [electronic resource]
... Bioinformatics for High Throughput Sequencing Naiara Rodríguez-Ezpeleta Ana M Aransay ● Michael Hackenberg Editors Bioinformatics for High Throughput Sequencing Editors ... different steps of a high throughput sequencing project The “newbies” eager to learn the basics of high throughput sequencing technologies and data analysis will find what they yearn for specially by ... Chicago, Chicago, IL, USA Institute for Genomics and Systems Biology, The University of Chicago, Chicago, IL, USA Irmtraud M Meyer Centre for High- Throughput Biology, University of British Columbia,...
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Báo cáo sinh học: "Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" doc
... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, ... author Abstract Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes ... modeling enables high- throughput analysis of complex nonlinear systems in various applications [1-2] In particular it was shown that generalized models can be used to obtain statistically highly-significant...
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Báo cáo hóa học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" pdf
... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, ... author Abstract Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes ... modeling enables high- throughput analysis of complex nonlinear systems in various applications [1-2] In particular it was shown that generalized models can be used to obtain statistically highly-significant...
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Báo cáo toán học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" ppt
... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, ... author Abstract Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes ... modeling enables high- throughput analysis of complex nonlinear systems in various applications [1-2] In particular it was shown that generalized models can be used to obtain statistically highly-significant...
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Báo cáo y học: "A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language" ppt
... et al Genome Biology 2011, 12:R8 http://genomebiology.com/2011/12/1/R8 consistent dataset for benchmarking LipidXplorer performance in interpreting spectra acquired on low- and highresolution ... this article as: Herzog et al.: A novel informatics concept for highthroughput shotgun lipidomics based on the molecular fragmentation query language Genome Biology 2011 12:R8 Submit your next manuscript ... spectrometer LipidXplorer imports spectra in generic mzXML format and converters from proprietary formats to mzXML are available for major instrument platforms Here we provide evidence that LipidXplorer...
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Báo cáo y học: "ISsaga is an ensemble of web-based methods for high throughput identification and semiautomatic annotation of insertion sequences in prokaryotic genomes" pdf
... automatic annotation Varani et al Genome Biology 2011, 12:R30 http://genomebiology.com/2011/12/3/R30 Page of Table Predictor performance Table Predictor performance (Continued) GB - IS + IS Manual ... is an ensemble of web-based methods for high throughput identification and semi-automatic annotation of insertion sequences in prokaryotic genomes Genome Biology 2011 12:R30 Submit your next ... performed using ISsaga can be found on the web site without registration Selected tabs that are important for understanding the description below are indicated in the accompanying text in the form:...
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báo cáo khoa học: " HAPIscreen, a method for high-throughput aptamer identification" pptx
... M1 and M2, were performed on the automated workstation For each SELEX, μM of the RNA library was heated at 80°C for min, cooled at 4°C for min, placed at room temperature for and mixed DNA primers ... immobilized on the two beads [20] (Figure 2A) Therefore monitoring the emission signal allows for the detection of complex formation We used this technology for monitoring the interactions between a candidate ... Alphascreen® signal indicating the formation of a highly stable ROP-R06-TAR kissing complex (Figure 3C) A ~10 fold higher AlphaScreen® signal was observed for this ternary complex in the presence...
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Báo cáo y học: "Establishment of a novel CCR5 and CXCR4 expressing line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists" pdf
... Page of 13 (page number not for citation purposes) Retrovirology 2004, Conclusions This new CD4+/CCR5+/CXCR4+ cell line described here is most valuable as a tool for high- throughput evaluation of ... it can be considered as a highly specific CXCR4 antagonist [26-28] It was demonstrated that two aspartate residues at positions 171 and 262 of CXCR4 are crucial for the high- affinity binding of ... concentrations (IC50) of SCH-C for inhibition of LD78β-induced calcium flux were ng/ml in the single and ng/ml in the double-transfected cells For AMD3100, the IC50 values for inhibition of SDF-1-induced...
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Báo cáo y học: "A method for high-throughput gene expression signature analysis" ppt
... as before, and polymerase chain reaction performed in a Thermo Electron (Milford, MA, USA) MBS 384 Satellite Thermal Cycler (initial denaturation of 92°C for minutes, 92°C for 30 s, 60°C for 30 ... very high throughput and dramatically lower cost, represents, in our view, the next transformative technology in the gene expression field We believe that the Genome Biology 2006, 7:R61 information ... μl), the plate covered, spun as before, heated at 95°C for two minutes and maintained at 50°C for six hours Unannealed probes were removed by centrifugation, as before A volume of μl of 1× Taq Ligase...
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Energy efficient technologies for high performance manufacturing industries
... Energy-Efficient Technologies for High- Performance Manufacturing Industries Cao Vinh Le B.Eng (Hons.), Nanyang Technological University, 2009 A DISSERTATION SUBMITTED FOR THE DEGREE OF DOCTOR ... truly admire his diligence and passion for high- quality and high- impact research which has been always a source of inspiration to me I also wish to thank him for forgiving and resolving so many troubles ... shortest path efficiently This dissertation presents novel energy-efficient technologies to fulfill the emerging green demands for high- performance manufacturing industries, which require manufactured products...
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Development and application of advanced proteomic techniques for high throughput identification of proteins
... (2005) Hu, Y., Uttamchandani, M & Yao, S.Q Microarray: a versatile platform for highthroughput functional proteomics Comb Chem High Throughput Screen 9, 203-212 (2006) x List of Tables Table 2.1 Proteins ... protein biotinylation strategies for potential high- throughput proteomics J Am Chem Soc 126, 10551062 (2004) Hu, Y., Chen, G.Y.J & Yao, S.Q Activity-based high throughput screening of enzymes ... from three different aspects for high- throughput identification of enzymes and their associated proteins in yeast proteome (catalomics) Firstly, to validate the high- throughput capacity of differential...
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Báo cáo y học: " Systems biology coupled with label-free high-throughput detection as a novel approach for diagnosis of chronic obstructive pulmonary disease" pptx
... examine only one strand of the information available about an organism Systems biology combines data from all these fields with bioinformatic, computational biology and engineering principles ... COPD Current Analytical Technologies Genes Proteins SYSTEMS BIOLOGY Small molecules RNA Figure puzzle Biology: beginning to piece together the life sciences Systems2 Systems Biology: beginning to ... opportunities for a more holistic approach not previously possible using conventional technologies such as the enzyme-linked immunosorbent assay (ELISA) [136-140] The implementation of these high- throughput...
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Developing high throughput amenable chemistry for chemical biology applications
... DEVELOPING HIGH- THROUGHPUT AMENABLE CHEMISTRY FOR CHEMICAL BIOLOGY APPLICATIONS RAJAVEL SRINIVASAN (M.Sc., Anna University, Chennai, India) A THESIS SUBMITTED FOR THE DOCTOR OF PHILOSOPHY ... results 33 2.4 High- throughput synthesis of 3250-member PTP inhibitor library 36 2.4.1 Traceless solid-phase synthesis of 325 azide fragments 38 2.4.2 General Procedure for the High- throughput ‘click’ ... 2007 Srinivasan, R.; “Highthroughput amenable Chemistry in Catalomics” A*STARNoyori Forum Joint Symposium on Organic Chemistry Singapore, May 2007 Srinivasan, R.; “Highthroughput amenable Chemistry...
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Tài liệu HEALTH EDUCATION THROUGH INFORMATION AND COMMUNICATION TECHNOLOGIES FOR K-8 STUDENTS: CELL BIOLOGY, MICROBIOLOGY, IMMUNOLOGY AND MICROSCOPY ppt
... 1984 recommended further curricular reforms in health education (AAMC, 1984) More specifically, it called for reduction of lectures, providing more time for independent study, requiring active ... settings, which are continuing education for health professionals and skillstraining for all individuals (Locatis, 2002) Continuing education is required for all health professionals in order to ... covering issues from different aspects of cells including cell biology, microbiology, immunology and microscopy In terms of cell biology, cell models are provided along with the cell cycle and...
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Báo cáo khoa học: TICL – a web tool for network-based interpretation of compound lists inferred by high-throughput metabolomics doc
... KEGGanim: pathway animations for high- throughput data Bioinformatics 24, 588–590 Antonov AV & Mewes HW (2006) Complex functionality of gene groups identified from high- throughput data J Mol Biol ... Metabolomics technology and bioinformatics Brief Bioinform 7, 128–139 Shulaev V & Oliver DJ (2006) Metabolic and proteomic markers for oxidative stress New tools for reactive oxygen species research ... great demand for bioinformatics to provide a statistically valid interpretation of compound lists produced experimentally Currently, several bioinformatics approaches are available for metabolomics...
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Báo cáo khoa học: "High Throughput Modularized NLP System for Clinical Text" pptx
... overall Kappa for the four topics were 0.59 for pulmonary fibrosis, 0.79 for cholangiocarcinoma, 0.79 for diabetes mellitus and 0.59 for congestive heart failure We ran a set of queries for each of ... 0.764487 0.769548 0.775346 Table Performance of different annotation methods The f-score results are fairly close for all methods; however, the recall is highest for the method that combines Metamap ... independent of other engines A total of 20 blade servers were configured for text processing The average document throughput for each blade was 20 documents per minute The text analysis engine was...
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Báo cáo hóa học: " Neuraminidase activity provides a practical read-out for a high throughput influenza antiviral screening assay" pptx
... confirmed the suitability of NA activity as a practical end-point index for development of a high throughput assay with rapid read-out for inhibition of virus replication This "accelerated" virus inhibition ... the likelihood of obtaining a product that is effective Unfortunately these latter viral inhibition assays are usually not suited to high throughput screening (HTS) In this report we describe modifications ... hemagglutinin (HA) gene segments from an avian virus source [5] early after infection This affords higher throughput with excellent signal/noise ratios, providing excellent assay sensitivity In addition...
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