... IL5 – the interleukin 5 gene, λIg – the λ-chain of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin 4 receptor ... effects of calpains in cancer cells [27-29] suggest that calpain participates in cell malignization by digesting the p53 tumor suppressor. Besides, calpains participate in induction of HIV ... Hodgkin’s disease; capn4: the calpain subunit 4 gene; ND4: the NADH dehydrogenase subunit 4 gene; IL5: the interleukin 5 gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of...
... tissue staining intensities influ-enced the measured values. Average value density measurements from individual Vgf immunoreactive dorsal spinal cord neurons, reflecting immunostaining intensity, ... in the serum, in the CSF, and in spinal cord motorneurons may be a biological diagnostic index for ALS. It remains to be directly tested whether restoring Vgf expressionin spinal cord motor ... sequencing, and the recombinant virus was packaged by infecting the PacI linearized recombinant viral DNA into human embryonic kidney (HEK)-293 cells (Clontech, CA). The resulting recom-binant...
... cannot determine the global players involved in such complex phenotypes. Integration of the gene expres-sion profiling with specific modulation of gene ex-pression in relevant signalling pathway ... according the defini-tions of the Illumina’s (ILL) data as well as of the Il-lumina’s annotations (table 2). Int. J. Med. Sci. 2009, 6 http://www.medsci.org 27weight cargo upon binding ... Genes detected and in following investigated for valida-tion A) an induced expression of the PKN1 gene B) a repressed expression of the ZCD1 gene C) a repressed expression of BSG gene...
... protein was released from the chitin column when the chitin-bound intein tag undergoes self-cleavage in the presence of DTT. Fig 12 showed chitin gel before (N1) and after (N2) releasing MCoTI-II. ... Aprotinin 4. Re MCoTI-II Fig 14. Checking the inhibiting activity of ReMCoTI-II by using diffusion method 1. PA from S. litula 2. PA of S. litula + ReMoTI-II 3. Trypsin 4. Trypsin + ReMCoTI-II ... expression: use LB medium containing 100µg/ml ampicillin, incubated in shakers at 37oC until the OD600 reached 0.6. Induction by IPTG. Expression and purification of recombinant MCoTI-II were followed...
... 2009)doi:10.1111/j.1742-4658.2009.06973.xActivating enhancer-binding protein (AP)-2e was previously described as anew regulator of integrin a10 expression in cartilage. In this study, we ana-lyzed the expression of AP-2e in differentiated ... the Sox9-binding sites of theAP-2e promoter (Sox9_1 and Sox9_2). Incubation of in vitro-synthesized Sox9 with the labeled oligonucleo-tides containing the Sox9-binding sites resulted in astrong ... Max-Planck-Institute of Biochemistry, Martinsried, GermanyThe family of activating enhancer-binding protein(AP)-2 transcription factors regulate their target genesthrough binding to the palindromic...
... of cytokineinduced expression of intercellular adhesion molecule 1(ICAM-1) in an intestinal epithelial cell line, a processblocked by proteasome inhibitors. Gut 42, 779–787.23 Lakshminarayanan ... to an increase in ICAM-1 expressionin Schwanncells of sciatic nervesAiguo Shen1,*, Junling Yang2,*, Yangyang Gu3, Dan Zhou4, Linlin Sun2, Yongwei Qin2,Jianping Chen2, Ping Wang2, ... LPS induces ICAM-1 expression in SCs, suggest that ICAM-1 may play a role in the focalaccumulation and antigen-induced activation of T cells in inflammatory demyelinating diseases of the PNS.As...
... forsilver staining, hot Coomassie s taining, ÔStains-AllÕ labellingor immunoblotting. T he following running conditions wereused: 60 m in at 1 00 V , 6 0 m in at 500 V, 60 min a t 1000 V,and ... thedeficiency in dystrophin o n the expression of Ca2+-bindingproteins. The relatively unique combination o f the pI valueand molecular mass of individual 2D spots can be useful in the initial ... carbocyanine dye ÔStains-AllÕ was u sed todetermine potential changes in the expression of majorCa2+-binding proteins in dystrophic fibres. A comparisonbetween the selective d ye labelling of...
... caveolin proteins [22]. Several studieshave shown that caveolin, a 21–24 kDa integral membraneprotein, plays a critical r ole in the regulation of cellproliferation, including SMC, by inhibiting ... by inhibiting signalling mol-ecules [ 45–47]. Caveolin-3 is the main isoform in musclecells, including SMC [30]. Caveolin-1 and caveolin-3 seem toact as scaffolding proteins that negatively ... nine passages a fter primaryculture. Confluent cells were maintained in quiescent modeby incubation for 24 h in serum-free m edium containing0.2% (w/v) fatty acid-free BSA. Then, SMC were incubatedor...
... induction of inducible nitricoxide synthase (iNOS), cyclo-oxygenase-2 (COX-2)and MMP-9. In this study, we investigated whetherLXR activation inhibits inducible TF expression andactivity in ... finallydegenerating into a lipid core. Plaque rupture exposesactive TF in the lipid core to circulating blood, trigger-ing thrombosis. As the initiator of coagulation, TF isa potential target for inhibiting ... the enhanced expression of in ammatory genes including TF duringthe development of atherosclerosis induced by the acti-vation of NFjB. The current data suggest that LXRsallow binding of NFjB...
... from both brain and intestine in L. maderae [14,15].The finding that N-terminally extended TKRPs arepredominantly expressed only in the gut and not in the brain are in line with findings of enrichment ... tachykinins, the hemokinins, were identified ona third precursor encoding gene, preprotachykinin C,expressed in hematopoietic cells of mouse, rat andhumans [6,7]. In invertebrates the tachykinins ... the insectatachykinin ⁄ tachykinin and insect kinin ⁄ Tyr-W-MIFneuropeptide family pairs. Ann NY Acad Sci 897,388–400.2Na¨ssel DR (1999) Tachykinin-related peptides in inver-tebrates: a...
... could also remain trapped and inhibit theintrinsic uncoupling activity of hUCP3, the digitonin treatedcells were extensively incubated with the resin Dowex-K21.This extracting procedure was ... times in NaCl/Pi,thenfixed in 4% (w/v) paraformaldehyde/NaCl/Pifor 60 min. Thecells were w ashed four times in NaCl/Piand then i ncubatedfor 60 min in NaCl/Picontaining 0.1Mglycine, ... permeabi-lized using 0.1% ( v/v) Triton X-100 in NaCl/Pifor 3 min,followed by four NaCl/Piwashes. The slides were thenincubated in blocking buffer containing 5% (v/v) fetalbovine serum, 0.1%...
... TICK FRDM SKIN; APPLICA- TIONS TO SKIN OF TURPENTINE, IODINE, R~'TONE; REMOVAL OF TICK BY INSERTION OF NEEDLE BETWEEN MOUTH PARTS; ASPIRIN FOR PAIN; ANTIBIOTIC TREATMENT IN- ~-~CTIVE. ... in CMIT, in order of increasing 'enT.ropy' The enl=ropy is given in P.he first column; ,':he ent.ries in ~h~ nezt ii columns are ~he percent of occurrences in the 11 disease ... correspond to the disease categories represented in CMIT. This is done by assign- ing terms which occur predominantly in one category to a single vocabulary and then sorting by entropy. Tables...
... believes in measuring quantitatively something of such an intrinsically subjective nature as self- expression in a discipline so detached from numbers and the right-wrong answer distinction ... teacher started assessing them according to the established marking criteria. Data analysis: Insights into students’ self -expression during the learning process Self -expression problems One ... very active in class discussions: expressing their wonder at the details of the text or disagreement with others’ ideas, defending their opinions convincingly, posing very interesting questions...
... thepresent study indicate that adipophilin protein expressionin muscle isinvolved in maintaining insulin sensitivity.AbbreviationsAdfp, adipophilin; CLB, classical lysis buffer; FA, fatty ... is implicated in insulin resistance. As shown in the present study,palmitic acid indeed impaired insulin signaling in C2C12 cells, which is in line with numerous studiesaddressing the effect ... protein expressionin muscle is increased,accompanied by indications for better insulin sensitiv-ity. Taken together, the results obtained in the presentstudy indicate that Adfp expression in...