lna based mirna microarrays and data analysis
Báo cáo y học: "Transition of healthy to diseased synovial tissue in rheumatoid arthritis is associated with gain of mesenchymal/fibrotic characteristics" pdf
... 56°C for 40 s, and 72°C for 30 s Data were analysed using Sequence Detector version 1.7 software (Applied Biosystems) Statistical analysis For statistical analysis, means and standard deviations ... cultures and real-time PCR and was responsible for statistical analysis, participated in the study design and drafted the manuscript TCAT was involved in acquisition of patient material and immunohistochemistry ... support and participated in the design of the study ACG-DG was involved in the design of the study and interpretation of data M CDR was involved in the design of the study and interpretation of data...
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Báo cáo y học: "Collagen-specific T-cell repertoire in blood and synovial fluid varies with disease activity in early rheumatoid arthritis" pot
... study, and analysis and interpretation of the data He drafted the manuscript and gave final approval of the version to be published RP, MDS, CN, GDS, AF and AB conducted the research MO and DA ... made substantial contributions to the conception and design of the study, and analysis and interpretation of the data He drafted the manuscript and gave final approval of the version to be published ... control and antigenstimulated sample is or greater Depletion and purity were assessed by flow cytometry and found consistently to exceed 95% and 70% in CD4+CD25+ (containing also Treg) depleted and...
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Báo cáo y học: "Post-translational aging of proteins in osteoarthritic cartilage and synovial fluid as measured by isomerized aspartate" pot
... manuscript, and the statistical analysis MB and RDZ coordinated and organized the primary sample collection and critically evaluated both the study design and the manuscript VBK conceived and designed ... designed the study, supervised the project, and assisted in both the statistical analysis and the manuscript preparation and editing All authors read and approved the final manuscript 10 11 12 ... normality, linearity, and homoscedasticity essential to methods of linear regression modeling, we logarithmically transformed IsoAsp, GAG, and protein values and used the D'Agostino and Pearson omnibus...
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Báo cáo y học: "Plasma and synovial fluid microRNAs as potential biomarkers of rheumatoid arthritis and osteoarthritis" pot
... indicate that synovial fluid miRNAs can be a useful tool for diagnosis of RA and OA, and for the analysis of their pathogenesis Plasma miRNAs and synovial fluid miRNAs correlate with clinical ... plasma and those in synovial fluid A and B, Plasma and synovial fluid concentrations of miR-16, miR-132, miR-146a, miR-155 and miR-223 in RA (A) and OA (B) The average concentrations of these miRNAs ... OA and HC D, Radar charts show the average concentrations of each miRNA of each sample Expression patterns of plasma miRNA of RA and OA were similar Synovial fluid miRNAs were similar to the miRNAs...
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Báo cáo y học: "Synovial effusion and synovial fluid biomarkers in psoriatic arthritis to assess intraarticular tumor necrosis factor-a blockade in the knee joint" doc
... Switzerland Authors’ contributions UF was responsible for the study concept and design, analysis and interpretation, and drafting the manuscript PS participated in the design of the study and performed ... were measured by computer-assisted morphometric analysis (Image Proplus version 5) and a mm square area was evaluated Statistical analysis Mean and standard deviations were used as descriptive statistics ... in the systemic biological (CRP: 0.86 ± 0.95 and 031 ± 0.26, P = 0.019) and clinical (DAS: 2.93 ± 0.43 and 2.24 ± 0.90, P = 0.002, mRAI: 7.50 ± 4.88 and 4.71 ± 5.37, P = 0.011) disease activity...
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Báo cáo y học: "Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254)" ppt
... investigated a wide range of synovial fluid and serum biochemical biomarkers, at two time points, to better understand the changes and damage which occur to both the bone and cartilage early in the course ... independent of treatment by both two-way Analysis of Variance (ANOVA) and by Mann-Whitney tests, so for the purposes of statistical analysis, we combined the drug and control groups to improve statistical ... and as described above, the drug and control groups were combined for purposes of statistical analysis Results Biomarker concentrations in the setting of acute knee injury To gain an understanding...
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Báo cáo y học: "Electrophoretic characterization of species of fibronectin bearing sequences from the N-terminal heparin-binding domain in synovial fluid samples from patients with osteoarthritis and rheumatoid arthritis" doc
... cluster in samples 1, 3, 4, 7, and (diagonal arrows pointing upward and to the left) (2) ~185-kDa (denoted by small brackets facing downward): OA samples and (b,d), and and (f,g) (blots/exposures ... 1, 3, 4, 7, and 8, as well as RA samples 11–15 (denoted by diagonal arrows pointing upward and to the left in Figs and 5; Table 1) In of the OA samples (1 and in Fig 4, also samples and in blots ... OA and RA SF samples The ratio of staining intensities for the ~200+-kDa bands as compared with the ~170-kDa bands was significantly greater in the 10 RA than in the OA samples (22.6 ± 35.0 and...
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Báo cáo y học: "Differential expression of RANK, RANK-L, and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils" doc
... (TNF) family and prevents apoptosis, increases survival, and stimulates cytokine production in dendritic cells [17,18] TRANCE and the ligand of RANK (RANKL) were originally cloned and sequenced ... pg/ml RANK-L and no OPG (n = 13) SF neutrophils obtained from patients with RA and incubated for up to days in CM (as described in Materials and methods and Figure 2a) or in SM (data not shown) ... EIA (data not shown) In contrast, PBMLs cultured under similar conditions secreted RANK-L and OPG in the supernatants (data not shown), confirming that neutrophils and PBMLs expressed RANK-L and...
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Báo cáo y học: "Extracellular mitochondrial DNA and oxidatively damaged DNA in synovial fluid of patients with rheumatoid arthritis" potx
... and with a concentration of 4.0 mM MgCl2 PCR analysis of extracellular mtDNA in the clinical samples The results of a typical PCR analysis of plasma and SF samples are shown in Fig Positive and ... The arbitrary cutoff threshold is based on comparisons between the intensities of PCR-amplified mtDNA fragments and the 676-bp band on each SDS–PAGE (see Fig 1) Data points above the cutoff threshold ... indicate mtDNA-positive samples, and data points below the cutoff threshold indicate mtDNAnegative samples Correlations of the relative band intensities between plasma and SF samples from individual...
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Báo cáo y học: "Osteogenic protein 1 in synovial fluid from patients with rheumatoid arthritis or osteoarthritis: relationship with disease and levels of hyaluronan and antigenic keratan sulfate" ppt
... contributions to the conception, design, analysis, interpretation, and writing of the data BSF was involved in the acquisition, analysis and interpretation of the data and in drafting the manuscript MM ... sample acquisition and analysis of the data BK was involved in the development and adaptation of the ELISA method for SF and in the acquisition of data CAM was involved in data acquisition EJMAT ... asymptomatic donors (donor) and from osteoarthritis (OA) and rheumatoid arthritis (RA) patients detected by an OP-1 sandwich ELISA [19] The data are presented as the mean ± standard error of the mean...
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Báo cáo y học: " Induction of multiple matrix metalloproteinases in human dermal and synovial fibroblasts by Staphylococcus aureus: implications in the pathogenesis of septic arthritis and other soft tissue infections" pdf
... the idea, designed the study, and prepared the manuscript draft AP, AK, KH, and DS all helped in the experimental design and data analysis and provided creative and critical suggestions after ... for hours for RNA analysis and 48 hours for protein analysis Fibroblasts were also treated with a combination of 10 μg each of rhIL-1β/TNF-α for hours and 48 hours For mRNA analysis, cells were ... and 2000, and 52% had a different primary site of infection identified, 70% of which were S aureuspositive and 17% of which were methicillin-resistant In a retrospective analysis by Moumile and...
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Báo cáo y học: " Effects on osteoclast and osteoblast activities in cultured mouse calvarial bones by synovial fluids from patients with a loose joint prosthesis and from osteoarthritis patients" pdf
... samples, and gave substantial input into data evaluation and manuscript preparation MJP participated in collection of patient samples and gave substantial input into data evaluation and manuscript ... AG (Basel, Switzerland), 1,25(OH)2-vitamin D3 (D3) by F Hoffmann-La Roche Ltd (Basel, Switzerland), and indomethacin by Merck Sharp & Dohme BV (Haarlem, The Netherlands) D3 and indomethacin were ... experiments Animal care and experiments were approved and conducted in accordance with accepted standards of humane animal care and use as deemed appropriate by the Animal Care and Use Committee of...
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Báo cáo y học: "Expression and regulation of CCL18 in synovial fluid neutrophils of patients with rheumatoid arthritis" potx
... analyzed for the expression of CC chemokines (a) and CXC, C and CX3C chemokines (b) Data obtained by Gene Pix™ Analysis Software were normalized, transformed and denoted as x-fold regulation versus the ... A, Vaslin B, Le Grand R, Brew BJ, Dormont D: Expression of chemokines and their receptors in human and simian astrocytes: evidence for a central role of TNFα and IFNγ in CXCR4 and CCR5 modulation ... than -1 and larger than +1 were significantly regulated Genes were clustered with the Database for Annotation, Visualization and Integrated Discovery (DAVID) software [26] Semiquantitative and quantitative...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
... analyzed the data, and wrote the manuscript RG conceived of the study, performed the design and coordination of the study, analyzed the data, and revised the manuscript HF, MY, SB, NB, and SS made ... diagnosis, conducted sampling procedures, and performed clinical and rheumatological data analyses BJ and JS participated in the design and coordination of the study, and drafted the manuscript MR has ... AH and AS analyzed microbiological and sequencing data, and revised the manuscript All authors read and approved the final manuscript 15 16 17 Acknowledgements We thank Sebastien Chaussonneri and...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... curves and data were normalized to the copy number of 18S RNA Expression of ACMSD isoforms in E coli pGEM-ACMSD I and pGEM-ACMSD II were digested with XhoI and Bpu1102I and cloned into pET-15b and ... swiss-pdbviewer and visual molecular dynamics [49] Acknowledgements We thank G Magni and S Ruggieri for helpful comments and suggestions and T Begley (Department of Chemistry and Chemical Biology, ... mixture), and l-kynurenine and 3-hydroxykynurenine (present at 0.1 mm concentration) were without effect On the other hand, a significant inhibition was exerted by quinolinic acid, picolinic acid and...
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Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx
... ligand affinity between Y7 and Y2 may prove very useful for studies of ligand–receptor interactions and 3D modeling, and we have previously been able to utilize differences between chicken and ... radioligand This radioligand had iodinated tyrosines at positions 21 and 27 and a specific activity of 4000 CiÆmmol)1 Saturation experiments were carried out with serial dilutions of radioligand, and ... Q8QGM3 The affinities of peptides and nonpeptidergic ligands for chicken Y7 were established through competition experiments with radioligand 125I-pPYY (Table and Fig 8) The most potent inhibitor...
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Báo cáo khoa học: Genomic organization, tissue distribution and deletion mutation of human pyridoxine 5¢-phosphate oxidase pptx
... (A) and mouse (B) PNPO gene Exons are designated by closed boxes, and introns by bold lines The ORF is marked black, and grey boxes denote the 5¢- and 3¢-UTR sequences The locations of CpG islands ... gel, and the product band was extracted from the gel, purified, and ligated into the pGEM vector Then a BamHI digested fragment was subcloned into pET28a expression vector (pET28a/PNPOx) and used ... contains CpG islands with a CGobs/CGexp ratio in excess of 0.6 and a G + C content of 62% spanning two regions from )377 to )158 and from )137 to +136 of the start codon Such a CpG island is indicative...
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báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
... MTC and HB conceived of the study and MTC and RD devised the experimental design for the scratch assays HB harvested the wound tissue in the OR and HE helped in logging de-identified clinical data ... and uncontaminated To assure viability and sterility, a vial was recovered from the freezer and passaged times and then tested for mycoplasmal, bacterial and fungal contaminants Six cultures are ... become the standardof-care in patients with diabetes and a foot ulcer, pressure ulcers and venous ulcers, to remove necrotic and infected tissue and stimulate healing In this study, we used debrided...
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báo cáo hóa học: " Soluble HLA measurement in saliva and cerebrospinal fluid in Caucasian patients with multiple sclerosis: a preliminary study" pdf
... assay included a standard curve derived from positive and negative controls Negative controls consisted of 2% BSA and human serum, free of sHLA-I and sHLA-II Positive control standards were prepared ... of soluble HLA-I and soluble HAL-II Drs Minagar and Kelley have contributed to this manuscript by recruiting and examining multiple sclerosis patients, interpretation of data and preparing the ... sHLA-I standards (100, 50, 25, 12.5, 6.25, 3.1 and ng/ml) of pure sHLA-I protein The test values were calculated from the curve described by these standards All sHLA-I assays were standardized...
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Báo cáo hóa học: " Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue" pdf
... responsiveness of HFF and explants to the TLR ligands The IL-8 response pattern to the TLR ligands was also different between HFF and ectocervical explants with only TLR3 and TLR4 ligands inducing IL-8 ... with LTA, CpG 2395, LPS, or Poly I:C (data not shown) TLR3 and TLR4 ligands inhibit HCMV infection in HFF After stimulation with TLR ligands, HFF were washed and infected with CMVPT30-gfp After ... with TLR3 and TLR4 ligands, but not TLR2 or TLR9 ligands, inhibited HCMV infection Time dependence of TLR stimulation for HCMV inhibition and IL-8 production The effect of timing of TLR-ligand exposure...
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