lectins cona and uea il with beehive honey and rj and with flower nectars used by the bees for honey and rj production
The 11th form non-english majors’ level of satisfaction with their reading comprehension lessons at phan boi chau specialized upper secondary school, nghe an
... background for the thesis Chapter 2: presents a detailed discussion of the method used in the study with all of its components encompassing the justification for using the quasi-experimental design, the ... and less effective learners were found in the number and range of strategies used, in how the strategies were applied to the task, and in whether they were appropriate for the task” (p.166) Therefore, ... on the same day with the researcher and another teacher and scored by the teacher-researcher - 24 - During the experiment, the experimental group was introduced and explained about CS, they...
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THE 11TH FORM NON ENGLISH MAJORS’ LEVEL OF SATISFACTION WITH THEIR READING COMPREHENSION LESSONS AT PHAN BOI CHAU SPECIALIZED UP
... French and Russian The school selects all the best teachers and students from school in the province The main duty of the school is to train and foster talents for the province and the country The ... comprehension lessons In fact, they motivate, keep the students’ interest in learning, help them understand and remember the reading text better and therefore can make them satisfy with the lessons Needless ... to teach their students, they will be able to understand the lessons mmore easily The more suitable and relevant the teaching and learning styles are to the students’ interests, habits and learning...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot
... PGs at the d and e bands in the gap zone of the fibrils formed by the quarter staggered array of type I collagen molecules, and the presence of keratan sulfate PGs at the a and c bands in the overlap ... significantly modify both the triple helical conformation of the trimeric species (Fig 3B) and the thermal stability, with the relevant exception of N-acetylation with acetic anhydride for the reasons mentioned ... effect The affinity of the binding peptides for decorin is in the nanomolar range with the same magnitude reported by others for type I collagen [47,48], and the dissociation constants are within...
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Báo cáo khoa học: Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia pdf
... the olfactory and the respiratory cilia [7] Expression of S100 proteins has been reported in the olfactory epithelium in teleosts and rodents [27,28], and in the cilia of human bronchial epithelial ... A2 and annexin A5 (A1, A2 and A5), together with the binding proteins of dicalcin (low-Ca2+ eluate), were electrophoresed on an SDS ⁄ PAGE gel, and the proteins were stained with silver (silver ... procedures) They were solubilized with the SDS ⁄ PAGE sample buffer, and were directly electrophoresed with known amounts of recombinant dicalcin and annexins The contents of annexins and dicalcin in the...
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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
... triplet sequences, the specificity of the observed effects confirms the higher selectivity of DAPI for the minor-groove of the ATT-triplet with respect to all the other individual strands of trinucleotide ... mechanisms: the shift in the maximum emission at a : drug/template molar ratio was about 12 and 15 nm, and the increase in the fluorescence intensity was only 2.2- and 1.6-fold for AAT- and random-template, ... a 30-base long GC-rich random sequence at the 5¢ end, as described in Materials and methods and illustrated in Fig 1A,B The conformational changes induced by DAPI on the different templates were...
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Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt
... °C for h To elute the proteins from the IgG beads, the NaCl concentration was increased to 150 mm and the mixture was incubated for another h at 16 °C The volume of the eluate was reduced by ... stage III and IV oocytes; S16 lysate was prepared after 24 h of incubation and used for an immunoprecipitation with anti-myc The RNA was eluted from the immunopellet and characterized for the presence ... above and analyzed by western blotting for the presence of known localization factors Input corresponds to 1% of the material used in the pulldown experiment The presence of the TAP-Vg1RBP band...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... perturbations of Ile102, Ile104, Cys115, Trp117, Lys118, Gly122 and Asn124 The error range is the standard deviation 2098 the peak intensities accurately (Fig S2) Therefore, the Kd values of the a sugar-binding ... measured for the main chain and amide proton groups of Ile102, Ile104, Cys115, Trp117, Lys118 and Gly122, and the side chain nitrogen and amide proton group of Asn124 in the c sugarbinding site These ... H4, and barely interacts with the region of H1 and the O-methyl group The crystal structure of EW29Ch with lactose showed that the O2, O3 and O4 atoms of the galactose residue of lactose formed...
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Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc
... inward The rates of oxygen uptake were deduced from the slopes of the tangents to the oxygraphic traces, and the contact points of the tangents with the curves were used to determine the O2 concentrations ... but rather as the product of reduction of O2 by INT, and the SOD-dependent inhibition of INT reduction appears to be an indirect effect On the other hand, the INT radical may generate by dismutation ... also accumulate by oxidation of formazan or by disproportionation of a mixture of formazan and the tetrazolium salt The optical spectra illustrated in Fig 2A were recorded before and after addition...
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Báo cáo y học: "Direct interaction of immunoglobulins with synovial fibroblasts: a missing link in the pathogenesis of rheumatoid arthritis" potx
... in the pathogenesis of disease [18] The present research on the interaction of RA-IgG and RASFs as well as other recent data, however, may change the picture It has been reported by Huizinga and ... IL- 16 levels correlated positively with the degree of joint destruction over a 2-year period [16] These data extend the aforementioned observations and link IL- 16 to the disease process of RA In this ... producers of IL- 16 in the rheumatoid joint, and it has been demonstrated that inflammatory cytokines present in the RA synovium such as TNFα and IL- 1β can upregulate IL1 6 in fibroblasts [21] By demonstrating...
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báo cáo khoa học: " Gene expression profiling in susceptible interaction of grapevine with its fungal pathogen Eutypa lata: Extending MapMan ontology for grapevine" pdf
... displays the informa- tion about the members of the gene families, the relative gene expression and the name of the enzyme in a single image It is interesting to observe that before véraison the pathway ... treatment and neither diagnostic tool available for this disease Therefore a better insight into the grapevine response to E lata infection is required While the gene model based on the genome ... created, one to be used with Operon microarrays and the other to be used http://www.biomedcentral.com/1471-2229/9/104 with Affymetrix microarrays Both final mapping files thus consist of the following...
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Interaction of EcoRI with noncognate DNA sequences computational investigation of dynamics of protein water and DNA conformation
... by the parmbsc0 force field [120] for the DNA and the Amber03 force field for the protein [121], and for water the TIP3P water model [122] was used The complex was first energy-minimized by the ... While many of the above studies focused on basepair step deformability, few studies have also focused on the deformability at the basepair level For example, Lankas et al [101] studied the deformability ... propounded the theory of the operon in 1961 [8] Their theory was based on their observations of induction of the lac gene The isolation and characterization of the lac repressor, a protein, and the...
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Interaction of scribble with zonula occludens and intermediate filament proteins
... repressed in the basal domain by the Scrib complex Conversely, the basal activity of the Scrib complex is antagonized by the Par and Crb complexes apically and therefore restricted to the basal ... al., 2004) These studies therefore indicate that Scrib functions in the 23 restriction of apical polarity determinants to the apical membrane and together with these, is necessary for the proper ... helices (A and B) The ligand binding groove is structurally formed by a connecting loop between A and B, B strand and B helix The connecting loop contains the conserved GLGF motif These residues...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... trap the oligomeric forms that not enter the gel, we used centrifugation assays Following the assay, we recovered a fraction of the protein in the pellet (lane 1, Fig 1B) and the remainder in the ... that the protein was subject to solublization not only by ssDNA (Fig 2A and B), but also by Mg2+ (Fig 3B), a common effect facilitated by oppositely charged ionic species On the other hand, hRad51 ... mM KCl either in the absence (lane 1–5) or presence of mM ATP (lane 6–10) and analysed by native PAGE (6% acrylamide) followed by silver staining Lane and has hRad51 (10 lM) without DNA and lane...
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Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray
... required for splicing, with the splicing activity occurring spontaneously inside the cell and affected primarily by the identity of amino acids at the splice junction Incubation of the cell with ... requiring neither cofactors nor auxiliary enzymes The elucidation of the splicing mechanism and the identification of the key amino acid residues involved in the scission and ligation of the peptide ... by Dawson et al in 1994,[15] and recently reviewed,[4] as a general synthetic route for the semi-synthesis of native proteins While many other ligation chemistries exist which result in the formation...
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Báo cáo y học: " Antagonistic interaction of HIV-1 Vpr with Hsf-mediated cellular heat shock response and Hsp16 in fission yeast (Schizosaccharomyces pombe)" potx
... in which the hsp16 gene is tagged with GFP and is under the control of its native promoter [35], demonstrated that both the wild type Vpr and the mutant protein (Vpr') elicited Hsp16 production ... down-regulation of hsp16 by Vpr is further evidenced by the results shown in Fig 5A, in which a GFP reporter was fused with the endogenous hsp16 promoter [35] and expression of vpr eliminated the Hsp16 elevation ... noticed in the examination of two Vpr mutations The R77Q and I74A mutants, which separate the apoptosis and G2 arrest induced by Vpr [24,54], showed no separation between the G2 induction and apoptosis...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... before and after the incubation with the manganese ions Fully active His120Asn and His145Asn variants exhibited about 53% and 95% of the corresponding wild-type activity, with the Km value for ... of the amino acid and the introduced aspartic residue at position 149 On the other hand, as agmatine lacks the a-carboxyl group, there would be no impediment for its binding and hydrolysis by the ... were 73, 82, 59 and 79 lgÆmL)1, for the wild-type, His120Asn, His145Asn and Asn149Asp variants, respectively The line in (B) is for the average of experimental values for all the enzyme variants...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... increases the probability of self-aggregation and therefore partially folded actin tends to aggregate Self-aggregation may be the reason for the irreversibility of transition from the native to the ... on the polymerization of intact actin 2D and 3D mutants mimic the phosphorylation of HSP25 by MAPKAP-2 kinase and form oligomers with smaller molecular mass than the wild type HSP25 [20] These ... Academy of Sciences) for the cloning and expression of recombinant forms of HSP25 and its mutants This investigation was supported by Russian Foundation for Basic Research and by the Wellcome Trust...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... previously for full-length TnrA [15] B subtilis 168 (wild-type) cells were grown in SMM medium supplemented with sodium nitrate until the late exponential growth phase, and the cells were then washed and ... resuspended in SMM without nitrate, and finally incubated for a further 20 Samples were taken before and after the shift, and soluble cell-free extract was prepared by ultracentrifugation The soluble ... of ATP binding to GlnK The raw data were fitted with a three-site binding model for a PII trimer The upper panel shows the raw data in the form of the heat effect during the titration of 25 lM...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... SSA by ELISA with negative results, and diluted with RPMI 1640 or NaCl/Pi (1 : 1, v/v) Blood samples were taken with the understanding and written consent of each subject Twenty millilitres of the ... of the former, which was similar for both complexes, wtSSA–Vb5.2 and SSA(C26S)– Vb5.2 Different concentrations of human Vb2.1 and Vb1 TCRs were also used to measure the binding to the immobilized ... demonstrates that the immobilization process affects the binding ability of the Vb5.2 in a similar manner as reported for TCR b chain, Vb8.2 [26] On the contrary, immobilization of monomer wtSSA and SSA(C26S)...
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Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx
... circumvented by the enzyme employing these molecules to metabolizing substrates A likely scenario for the production of these molecules involves the partial reduction of O2 caused by 2412 Fig ... both catalase and peroxidase activities, and the studies by Wood et al [20] showing the enzyme to be activated by low concentrations of H2O2 Unquestionably, the generation of H2O2 and other ROI during ... GSH, reduced glutathione compounds, and reducing agents, the activities of melanogenic enzymes and competitive oxidases, and the availability of enzyme cofactors The two-step reaction sequence that...
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